Partial purification of the template-active fraction of chromatin: A preliminary report (1974)
Gottesfeld, J. M., Garrard, W. T., Bagi, G., Wilson, R. F., Bonner, James
A fraction of rat-liver chromatin that is transcriptionally active in vivo has been purified 6- to 7-fold over whole chromatin. This was accomplished by selectively shearing chromatin with DNase II...
George, J B, Li, S, Garrard, W T
The immunoglobulin kappa gene locus encodes 95% of the light chains of murine antibody molecules and is thought to contain up to 300 variable (V kappa)-region genes generally considered to comprise...
Fishel, B R, Sperry, A O, Garrard, W T
Chromatin becomes reorganized during mitosis each cell cycle. To identify genes potentially involved in these supramolecular events, we have used a colony-color assay to screen temperature-sensitive...
DNase I-hypersensitive sites represent "nucleosome-free" regions in chromatin where the underlying DNA sequence is highly accessible to trans-acting proteins. Here we demonstrate that it is possible...
Positive DNA supercoiling generates a chromatin conformation characteristic of highly active genes.
During transcription, positive DNA supercoils generated ahead of RNA polymerase could theoretically uncoil the negative DNA supercoils associated with nucleosomes and thereby decondense the chromatin...
To address the role of transient torsional stress in transcription, we have utilized the regulated expression of HO endonuclease in yeast to create double-strand breaks in DNA templates in vivo at...
Nitrate Reductase and Soluble Cytochrome c in Spirillum itersonii
Gauthier, Dorothy K., Clark-Walker, G. D., Garrard, W. T., Lascelles, June
The addition of nitrate to cultures of Spirillum itersonii incubated under low aeration produced a diauxic growth pattern in which the second exponential phase was preceded by the appearance of...
Treatment of Spirillum itersonii with tris(hydroxymethyl)aminomethane (Tris)-ethylenediaminetetraacetate (EDTA) results in the quantitative release of alkaline phosphatase and ribonuclease into the...
Pommier, Y, Cockerill, P N, Kohn, K W, Garrard, W T
We demonstrate that the simian virus 40 genome contains a single MAR (matrix association region) that maps within a large T-antigen coding region (nucleotides 4071 to 4377). This region contains...
Sperry, A O, Blasquez, V C, Garrard, W T
A family of A + T-rich sequences termed MARs ("matrix association regions") mediate chromosomal loop attachment. Here we demonstrate that several MARs both specifically bind and contain multiple...
Reudelhuber, T L, Ball, D J, Davis, A H, Garrard, W T
Electrophoresis fractionates nucleosomes which possess different protein compositions. We report here a procedure for transferring the DNA components of electrophoretically resolved nucleosomes to...
The active immunoglobulin kappa chain gene is packaged by non-ubiquitin-conjugated nucleosomes.
Huang, S Y, Barnard, M B, Xu, M, Matsui, S, Rose, S M, Garrard, W T
To elucidate the molecular features of active chromatin, we have mapped, by two-dimensional electrophoresis, the protein composition of nucleosomes that package the immunoglobulin kappa chain gene of...
Points of contact between histone H1 and the histone octamer.
Boulikas, T, Wiseman, J M, Garrard, W T
The topography of the interaction between histone H1 and the histone octamer has been investigated. Bovine thymus nuclei or enzymatically fragmented chromatin were treated...
Basal-level expression of the yeast HSP82 gene requires a heat shock regulatory element.
McDaniel, D, Caplan, A J, Lee, M S, Adams, C C, Fishel, B R, Gross, D S, ...
Previous studies have shown that heat shock factor is constitutively bound to heat shock elements in Saccharomyces cerevisiae. We demonstrate that mutation of the heat shock element closest to the...
The potential Z-forming sequence (dT-dG)n . (dC-dA)n is an abundant, interspersed repeat element that is ubiquitous in eucaryotic nuclear genomes. We report that in contrast to eucaryotic nuclear...
5-methylcytosine is localized in nucleosomes that contain histone H1.
Ball, D J, Gross, D S, Garrard, W T
We have developed a procedure for purifying highly specific polyclonal antibodies against 5-methylcytosine. These antibodies were used to probe the distribution of 5-methylcytosine among fractionated...
Partial Purification of the Template-Active Fraction of Chromatin: A Preliminary Report
Gottesfeld, J. M., Garrard, W. T., Bagi, G., Wilson, R. F., Bonner, James
A fraction of rat-liver chromatin that is transcriptionally active in vivo has been purified 6- to 7-fold over whole chromatin. This was accomplished by selectively shearing chromatin with DNase II...
Utilizing yeast strains containing promoter mutations, we demonstrate that transcription of the HSP82 gene causes nucleosomes toward the 3'-end to become DNase I sensitive and 'split' into structures...
George, J B, Li, S, Garrard, W T
The immunoglobulin kappa gene locus encodes 95% of the light chains of murine antibody molecules and is thought to contain up to 300 variable (V kappa)-region genes generally considered to comprise...
Fishel, B R, Sperry, A O, Garrard, W T
Chromatin becomes reorganized during mitosis each cell cycle. To identify genes potentially involved in these supramolecular events, we have used a colony-color assay to screen temperature-sensitive...
DNase I-hypersensitive sites represent "nucleosome-free" regions in chromatin where the underlying DNA sequence is highly accessible to trans-acting proteins. Here we demonstrate that it is possible...
Positive DNA supercoiling generates a chromatin conformation characteristic of highly active genes.
During transcription, positive DNA supercoils generated ahead of RNA polymerase could theoretically uncoil the negative DNA supercoils associated with nucleosomes and thereby decondense the chromatin...
To address the role of transient torsional stress in transcription, we have utilized the regulated expression of HO endonuclease in yeast to create double-strand breaks in DNA templates in vivo at...
Nitrate Reductase and Soluble Cytochrome c in Spirillum itersonii
Gauthier, Dorothy K., Clark-Walker, G. D., Garrard, W. T., Lascelles, June
The addition of nitrate to cultures of Spirillum itersonii incubated under low aeration produced a diauxic growth pattern in which the second exponential phase was preceded by the appearance of...
Treatment of Spirillum itersonii with tris(hydroxymethyl)aminomethane (Tris)-ethylenediaminetetraacetate (EDTA) results in the quantitative release of alkaline phosphatase and ribonuclease into the...
Pommier, Y, Cockerill, P N, Kohn, K W, Garrard, W T
We demonstrate that the simian virus 40 genome contains a single MAR (matrix association region) that maps within a large T-antigen coding region (nucleotides 4071 to 4377). This region contains...
Sperry, A O, Blasquez, V C, Garrard, W T
A family of A + T-rich sequences termed MARs ("matrix association regions") mediate chromosomal loop attachment. Here we demonstrate that several MARs both specifically bind and contain multiple...
Reudelhuber, T L, Ball, D J, Davis, A H, Garrard, W T
Electrophoresis fractionates nucleosomes which possess different protein compositions. We report here a procedure for transferring the DNA components of electrophoretically resolved nucleosomes to...
The active immunoglobulin kappa chain gene is packaged by non-ubiquitin-conjugated nucleosomes.
Huang, S Y, Barnard, M B, Xu, M, Matsui, S, Rose, S M, Garrard, W T
To elucidate the molecular features of active chromatin, we have mapped, by two-dimensional electrophoresis, the protein composition of nucleosomes that package the immunoglobulin kappa chain gene of...
Points of contact between histone H1 and the histone octamer.
Boulikas, T, Wiseman, J M, Garrard, W T
The topography of the interaction between histone H1 and the histone octamer has been investigated. Bovine thymus nuclei or enzymatically fragmented chromatin were treated...
Basal-level expression of the yeast HSP82 gene requires a heat shock regulatory element.
McDaniel, D, Caplan, A J, Lee, M S, Adams, C C, Fishel, B R, Gross, D S, ...
Previous studies have shown that heat shock factor is constitutively bound to heat shock elements in Saccharomyces cerevisiae. We demonstrate that mutation of the heat shock element closest to the...
The potential Z-forming sequence (dT-dG)n . (dC-dA)n is an abundant, interspersed repeat element that is ubiquitous in eucaryotic nuclear genomes. We report that in contrast to eucaryotic nuclear...
5-methylcytosine is localized in nucleosomes that contain histone H1.
Ball, D J, Gross, D S, Garrard, W T
We have developed a procedure for purifying highly specific polyclonal antibodies against 5-methylcytosine. These antibodies were used to probe the distribution of 5-methylcytosine among fractionated...
Partial Purification of the Template-Active Fraction of Chromatin: A Preliminary Report
Gottesfeld, J. M., Garrard, W. T., Bagi, G., Wilson, R. F., Bonner, James
A fraction of rat-liver chromatin that is transcriptionally active in vivo has been purified 6- to 7-fold over whole chromatin. This was accomplished by selectively shearing chromatin with DNase II...
Utilizing yeast strains containing promoter mutations, we demonstrate that transcription of the HSP82 gene causes nucleosomes toward the 3'-end to become DNase I sensitive and 'split' into structures...