Phosphorylation and nuclear localization of the varicella-zoster virus gene 63 protein.
Stevenson, D, Xue, M, Hay, J, Ruyechan, W T
The protein encoded by varicella-zoster virus open reading frame 63 and carboxy-terminal deletions of the same were expressed either as fusion proteins at the carboxy terminus of the maltose-binding...
Shelton, L S, Albright, A G, Ruyechan, W T, Jenkins, F J
The UL37 and ICP8 proteins present in herpes simplex virus type 1 (HSV-1)-infected-cell extracts produced at 24 h postinfection coeluted from single-stranded-DNA-cellulose columns. Experiments...
Perera, L P, Mosca, J D, Ruyechan, W T, Hayward, G S, Straus, S E, Hay, J
Accumulating evidence indicates that the product of the putative immediate-early gene ORF62 (IE62) activates varicella-zoster virus (VZV) genes thought to represent all three kinetic classes, namely,...
Sabella, C, Lowry, P W, Abbruzzi, G M, Koropchak, C M, Kinchington, P R, Sadegh-Zadeh, M, ...
The IE62 protein, the primary regulatory protein of varicella-zoster virus (VZV) and the major component of the virion tegument, was an effective immunogen in the guinea pig model of VZV infection,...
The varicella-zoster virus immediate-early protein IE62 is a major component of virus particles.
Kinchington, P R, Hougland, J K, Arvin, A M, Ruyechan, W T, Hay, J
Varicella-zoster virus (VZV) open reading frame (ORF) 62 potentially encodes a protein with considerable amino acid homology to the herpes simplex virus (HSV) immediate-early regulatory polypeptide...
Modification of the herpes simplex virus type 1 major DNA-binding protein (ICP8) with reagents and conditions specific for arginine, lysine, and tyrosine residues indicates that surface lysine and...
Transcription from varicella-zoster virus gene 67 (glycoprotein IV).
Ling, P, Kinchington, P R, Sadeghi-Zadeh, M, Ruyechan, W T, Hay, J
Three transcripts map to the varicella-zoster virus (VZV) open reading frame (ORF) 67, which encodes glycoprotein IV (gpIV). All of these transcripts are polyadenylated and are transcribed from left...
Kinchington, P R, Ling, P, Pensiero, M, Moss, B, Ruyechan, W T, Hay, J
Many characteristics of the putative protein encoded by varicella-zoster virus (VZV) open reading fram (ORF) 14 indicate that it is a glycoprotein, which has been designated gpV. To identify the...
Inversion and circularization of the varicella-zoster virus genome.
Kinchington, P R, Reinhold, W C, Casey, T A, Straus, S E, Hay, J, Ruyechan, W T
The genome of varicella-zoster virus (VZV) is a linear, double-stranded molecule of DNA composed of a long (L) region covalently linked to a short (S) region. The S region is capable of inverting...
In vitro characterization of a thermolabile herpes simplex virus DNA-binding protein.
Ruyechan, W T, Chytil, A, Fisher, C M
The major herpes simplex virus DNA-binding protein, ICP8, was purified from cells infected with the herpes simplex virus type 1 temperature-sensitive strain tsHA1. tsHA1 ICP8 bound single-stranded...
Kinchington, P R, Remenick, J, Ostrove, J M, Straus, S E, Ruyechan, W T, Hay, J
A strain variation of varicella-zoster virus that maps to the UL region of the genome was found to be due to different copy numbers of a high GC 42-base-pair repeat. DNA sequence analysis of this...
Haffey, M L, Stevens, J T, Terry, B J, Dorsky, D I, Crumpacker, C S, Wietstock, S M, ...
The herpes simplex virus type 1 (HSV-1) (strain 17) DNA polymerase gene has been cloned into an Escherichia coli-yeast shuttle vector fused to the galactokinase gene (GAL-1) promoter. Genes...
Kinchington, P R, Inchauspe, G, Subak-Sharpe, J H, Robey, F, Hay, J, Ruyechan, W T
We have identified, in varicella-zoster virus (VZV)-infected cells, the product of the gene predicted to code for the VZV analog of the herpes simplex virus major DNA-binding protein. The open...
The major herpes simplex virus DNA-binding protein, designated ICP8, binds tightly to single-stranded DNA and is required for replication of viral DNA. The sensitivity of the DNA-binding activity of...
Holmes, A M, Wietstock, S M, Ruyechan, W T
A novel DNA primase activity has been identified in HeLa cells infected with herpes simplex virus type 1 (HSV-1). Such an activity has not been detected in mock-infected cells. The primase activity...
The interaction of the herpes simplex virus type 1 (HSV-1) major DNA-binding protein, infected-cell polypeptide 8 (ICP8), with nucleic acids has been examined by a filter-binding assay and electron...
Casey, T A, Ruyechan, W T, Flora, M N, Reinhold, W, Straus, S E, Hay, J
A strain variation in the internal and terminal repeats which bind the short unique sequence of varicella-zoster virus (VZV) DNA was found to be due to an insertion or deletion of DNA sequences at a...
DNA-binding proteins present in varicella-zoster virus-infected cells.
Roberts, C R, Weir, A C, Hay, J, Straus, S E, Ruyechan, W T
DNA-binding proteins present in varicella-zoster virus-infected cells were identified by DNA-cellulose chromatography of radioactively labeled cell extracts. Seven virus-specific proteins, ranging in...
Properties of the major DNA-binding protein found in herpes simplex virus-infected cells were investigated by using a filter binding assay and electron microscopy. Filter binding indicated that the...
Electron microscopic study of equine herpesvirus type 1 DNA.
Ruyechan, W T, Dauenhauer, S A, O'Callaghan, D J
Electron microscopic studies of equine herpesvirus DNA revealed that single strands that were allowed to reanneal formed single-stranded loops with double-stranded stems only at one end of the...
Structure of varicella-zoster virus DNA.
Straus, S E, Aulakh, H S, Ruyechan, W T, Hay, J, Casey, T A, Vande Woude, G F, ...
Varicella-zoster virus (VZV) DNA was prepared from nucleocapsids and from enveloped virions of a laboratory strain (Ellen) and directly from the vesicle fluids of patients with zoster infections. VZV...
Green, B A, Neill, R J, Ruyechan, W T, Holmes, R K
Escherichia coli SA53 produces a new enterotoxin that has a biological activity similar to that of E. coli heat-labile enterotoxin (LT) but is not neutralized by antiserum against LT or cholera...
Regulation of varicella-zoster virus gene expression in human T lymphocytes.
Perera, L P, Mosca, J D, Ruyechan, W T, Hay, J
Varicella-zoster virus (VZV), a neurotropic alphaherpesvirus, is the etiologic agent of chicken pox and shingles (zoster) in humans. Using an in vitro transient expression assay, we have evaluated...
Molecular cloning and physical mapping of varicella-zoster virus DNA.
Straus, S E, Owens, J, Ruyechan, W T, Takiff, H E, Casey, T A, Vande Woude, G F, ...
Varicella-zoster virus (VZV) DNA was cleaved with restriction endonuclease EcoRI, and most of the resulting fragments were successfully cloned in the phage vector lambda gtWES . lambda B. Double...
Knipe, D M, Ruyechan, W T, Roizman, B
We have transferred a genetic locus determining resistance to phosphonoacetic acid (PAAr) from one herpes simplex viral genome to another by two methods of marker transfer. One method requires...
Phosphorylation and nuclear localization of the varicella-zoster virus gene 63 protein.
Stevenson, D, Xue, M, Hay, J, Ruyechan, W T
The protein encoded by varicella-zoster virus open reading frame 63 and carboxy-terminal deletions of the same were expressed either as fusion proteins at the carboxy terminus of the maltose-binding...
Shelton, L S, Albright, A G, Ruyechan, W T, Jenkins, F J
The UL37 and ICP8 proteins present in herpes simplex virus type 1 (HSV-1)-infected-cell extracts produced at 24 h postinfection coeluted from single-stranded-DNA-cellulose columns. Experiments...
Perera, L P, Mosca, J D, Ruyechan, W T, Hayward, G S, Straus, S E, Hay, J
Accumulating evidence indicates that the product of the putative immediate-early gene ORF62 (IE62) activates varicella-zoster virus (VZV) genes thought to represent all three kinetic classes, namely,...
Sabella, C, Lowry, P W, Abbruzzi, G M, Koropchak, C M, Kinchington, P R, Sadegh-Zadeh, M, ...
The IE62 protein, the primary regulatory protein of varicella-zoster virus (VZV) and the major component of the virion tegument, was an effective immunogen in the guinea pig model of VZV infection,...
The varicella-zoster virus immediate-early protein IE62 is a major component of virus particles.
Kinchington, P R, Hougland, J K, Arvin, A M, Ruyechan, W T, Hay, J
Varicella-zoster virus (VZV) open reading frame (ORF) 62 potentially encodes a protein with considerable amino acid homology to the herpes simplex virus (HSV) immediate-early regulatory polypeptide...
Modification of the herpes simplex virus type 1 major DNA-binding protein (ICP8) with reagents and conditions specific for arginine, lysine, and tyrosine residues indicates that surface lysine and...
Transcription from varicella-zoster virus gene 67 (glycoprotein IV).
Ling, P, Kinchington, P R, Sadeghi-Zadeh, M, Ruyechan, W T, Hay, J
Three transcripts map to the varicella-zoster virus (VZV) open reading frame (ORF) 67, which encodes glycoprotein IV (gpIV). All of these transcripts are polyadenylated and are transcribed from left...
Kinchington, P R, Ling, P, Pensiero, M, Moss, B, Ruyechan, W T, Hay, J
Many characteristics of the putative protein encoded by varicella-zoster virus (VZV) open reading fram (ORF) 14 indicate that it is a glycoprotein, which has been designated gpV. To identify the...
Inversion and circularization of the varicella-zoster virus genome.
Kinchington, P R, Reinhold, W C, Casey, T A, Straus, S E, Hay, J, Ruyechan, W T
The genome of varicella-zoster virus (VZV) is a linear, double-stranded molecule of DNA composed of a long (L) region covalently linked to a short (S) region. The S region is capable of inverting...
In vitro characterization of a thermolabile herpes simplex virus DNA-binding protein.
Ruyechan, W T, Chytil, A, Fisher, C M
The major herpes simplex virus DNA-binding protein, ICP8, was purified from cells infected with the herpes simplex virus type 1 temperature-sensitive strain tsHA1. tsHA1 ICP8 bound single-stranded...
Kinchington, P R, Remenick, J, Ostrove, J M, Straus, S E, Ruyechan, W T, Hay, J
A strain variation of varicella-zoster virus that maps to the UL region of the genome was found to be due to different copy numbers of a high GC 42-base-pair repeat. DNA sequence analysis of this...
Haffey, M L, Stevens, J T, Terry, B J, Dorsky, D I, Crumpacker, C S, Wietstock, S M, ...
The herpes simplex virus type 1 (HSV-1) (strain 17) DNA polymerase gene has been cloned into an Escherichia coli-yeast shuttle vector fused to the galactokinase gene (GAL-1) promoter. Genes...
Kinchington, P R, Inchauspe, G, Subak-Sharpe, J H, Robey, F, Hay, J, Ruyechan, W T
We have identified, in varicella-zoster virus (VZV)-infected cells, the product of the gene predicted to code for the VZV analog of the herpes simplex virus major DNA-binding protein. The open...
The major herpes simplex virus DNA-binding protein, designated ICP8, binds tightly to single-stranded DNA and is required for replication of viral DNA. The sensitivity of the DNA-binding activity of...
Holmes, A M, Wietstock, S M, Ruyechan, W T
A novel DNA primase activity has been identified in HeLa cells infected with herpes simplex virus type 1 (HSV-1). Such an activity has not been detected in mock-infected cells. The primase activity...
The interaction of the herpes simplex virus type 1 (HSV-1) major DNA-binding protein, infected-cell polypeptide 8 (ICP8), with nucleic acids has been examined by a filter-binding assay and electron...
Casey, T A, Ruyechan, W T, Flora, M N, Reinhold, W, Straus, S E, Hay, J
A strain variation in the internal and terminal repeats which bind the short unique sequence of varicella-zoster virus (VZV) DNA was found to be due to an insertion or deletion of DNA sequences at a...
DNA-binding proteins present in varicella-zoster virus-infected cells.
Roberts, C R, Weir, A C, Hay, J, Straus, S E, Ruyechan, W T
DNA-binding proteins present in varicella-zoster virus-infected cells were identified by DNA-cellulose chromatography of radioactively labeled cell extracts. Seven virus-specific proteins, ranging in...
Properties of the major DNA-binding protein found in herpes simplex virus-infected cells were investigated by using a filter binding assay and electron microscopy. Filter binding indicated that the...
Electron microscopic study of equine herpesvirus type 1 DNA.
Ruyechan, W T, Dauenhauer, S A, O'Callaghan, D J
Electron microscopic studies of equine herpesvirus DNA revealed that single strands that were allowed to reanneal formed single-stranded loops with double-stranded stems only at one end of the...
Structure of varicella-zoster virus DNA.
Straus, S E, Aulakh, H S, Ruyechan, W T, Hay, J, Casey, T A, Vande Woude, G F, ...
Varicella-zoster virus (VZV) DNA was prepared from nucleocapsids and from enveloped virions of a laboratory strain (Ellen) and directly from the vesicle fluids of patients with zoster infections. VZV...
Green, B A, Neill, R J, Ruyechan, W T, Holmes, R K
Escherichia coli SA53 produces a new enterotoxin that has a biological activity similar to that of E. coli heat-labile enterotoxin (LT) but is not neutralized by antiserum against LT or cholera...
Regulation of varicella-zoster virus gene expression in human T lymphocytes.
Perera, L P, Mosca, J D, Ruyechan, W T, Hay, J
Varicella-zoster virus (VZV), a neurotropic alphaherpesvirus, is the etiologic agent of chicken pox and shingles (zoster) in humans. Using an in vitro transient expression assay, we have evaluated...
Molecular cloning and physical mapping of varicella-zoster virus DNA.
Straus, S E, Owens, J, Ruyechan, W T, Takiff, H E, Casey, T A, Vande Woude, G F, ...
Varicella-zoster virus (VZV) DNA was cleaved with restriction endonuclease EcoRI, and most of the resulting fragments were successfully cloned in the phage vector lambda gtWES . lambda B. Double...
Knipe, D M, Ruyechan, W T, Roizman, B
We have transferred a genetic locus determining resistance to phosphonoacetic acid (PAAr) from one herpes simplex viral genome to another by two methods of marker transfer. One method requires...