Huang, W, Johnston, WA, Hayes, MA, De Voss, JJ, Gillam, EMJ
Cytochrome P450 (CYP) enzymes involved in mammalian xenobiotic metabolism are attractive targets for the engineering of biocatalysts since they have broad and overlapping substrate and reaction...
A shuffled CYP1A library shows both structural integrity and functional diversity (2007)
Johnston, WA, Huang, W, De Voss, JJ, Hayes, MA, Gillam, EMJ
The cytochrome P450 enzymes (P450s) that mediate mammalian xenobiotic metabolism are highly versatile monooxygenases, which show wide and overlapping substrate ranges but generally poor catalytic...
Extending the diversity of cytochrome P450 enzymes by DNA family shuffling (2007)
Rosic, NN, Huang, WL, Johnston, WA, De Voss, JJ, Gillam, EMJ
The cytochrome P450 enzymes involved in xenobiotic metabolism are an excellent starting point for the directed evolution of novel biocatalysts due to their wide substrate specificity. A shuffled...
Johnston, WA, Stewart, M, Lee, P, Cooney, MJ
DO-transient nutrient controllers use the dissolved oxygen signal to attempt acetate threshold tracking during fed-batch cultivation of recombinant E coli. Here we apply DO-transient control to the...