Walter Kolch

Computational modelling of cancerous mutations in the EGFR/ERK signalling pathway (2009)

Orton, Richard J, Adriaens, Michiel E, Gormand, Amelie, Sturm, Oliver E, Kolch, Walter, Gilbert, David R

Abstract Background The Epidermal Growth Factor Receptor (EGFR) activated Extracellular-signal Regulated Kinase (ERK) pathway is a critical cell signalling pathway that relays the signal for a cell...

Dynamics of receptor and protein transducer homodimerisation (2008)

Vera, Julio, Millat, Thomas, Kolch, Walter, Wolkenhauer, Olaf

Abstract Background Signalling pathways are complex systems in which not only simple monomeric molecules interact, but also more complex structures that include constitutive or induced protein...

REVIEW ARTICLE Computational modelling of the receptor-tyrosine-kinase-activated MAPK pathway (2008)

Richard J. Orton, Oliver E. Sturm, Vladislav Vyshemirsky, Muffy Calder, David R. Gilbert, Walter Kolch

The MAPK (mitogen-activated protein kinase) pathway is one of the most important and intensively studied signalling pathways. It is at the heart of a molecular-signalling network that governs the...

Full Title Computational Modelling of the Receptor Tyrosine Kinase Activated MAPK Pathway Authors Names (2008)

Richard J. Orton, Oliver E. Sturm, Vladislav Vyshemirsky, Muffy Calder, David R. Gilbert, Walter Kolch, ...

The Mitogen Activated Protein Kinase (MAPK) pathway is one of the most important and intensively studied signalling pathways. It is at the heart of a molecular signalling network that governs the...

Investigating the correspondence between transcriptomic and proteomic expression profiles using coupled cluster models (2008)

Rogers, Simon, Girolami, Mark, Kolch, Walter, Waters, Katrina M., Liu, Tao, Thrall, Brian, ...

Motivation: Modern transcriptomics and proteomics enable us to survey the expression of RNAs and proteins at large scales. While these data are usually generated and analyzed separately, there is an...

A database of naturally occurring human urinary peptides and proteins for use in clinical applications (2007)

Petra Zürbig, Joshua Coon, Hartwig Bauer, Georg Behrens, Mohammed Dakna, Anna Dominiczak, ...

Owing to its availability, ease of collection and correlation with (patho-) physiology, urine is an attractive source for clinical proteomics. However, the lack of comparable datasets from large...

The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity (2006)

Gloeckner, Christian Johannes, Kinkl, Norbert, Schumacher, Annette, Braun, Ralf J., O'Neill, Eric, Meitinger, Thomas, ...

Mutations in the leucine-rich repeat kinase 2 gene (LRRK2) have been recently identified in families with autosomal dominant late-onset Parkinson disease (PD). The LRRK2 protein consists of multiple...

D.: When kinases meet mathematics: the systems biology of MAPK signalling (2005)

Muffy Calder, David Gilbert, Walter Kolch, Walter Kolch

The MAPK/ERK pathway regulates fundamental cellular function such as cell proliferation, survival, differentiation and motility, raising the question how these diverse functions are specified and...

The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity (2005)

Gloeckner, Christian Johannes, Kinkl, Norbert, Schumacher, Annette, Braun, Ralf J., O'Neill, Eric, Meitinger, Thomas, ...

Mutations in the leucine-rich repeat kinase 2 gene (LRRK2) have been recently identified in families with autosomal dominant late-onset Parkinson disease (PD). The LRRK2 protein consists of multiple...

Modelling and simulation of intracellular dynamics: Choosing an appropriate framework (2004)

Olaf Wolkenhauer, Mukhtar Ullah, Walter Kolch, Kwang-hyun Cho

Abstract--- Systems biology, that is, mathematical modelling and simulation of biochemical reaction networks in intracellular processes has gained renewed interest in recent years. For most...

Experimental design in systems biology, based on parameter sensitivity analysis using a Monte Carlo method: A case study for the TNFα-mediated NF-κB signal transduction pathway (2003)

Kwang-hyun Cho, Walter Kolch, Olaf Wolkenhauer

Mathematical modeling and dynamic simulation of signal transduction pathways is a central theme in systems biology and is increasingly attracting attention in the postgenomic era. The estimation of...

Mathematical modeling of the influence of RKIP on the ERK signaling pathway (2003)

Kwang-hyun Cho, Sung-young Shin, Hyun-woo Kim, Olaf Wolkenhauer, Brian Mcferran, Walter Kolch

Abstract. This paper investigates the influence of the Raf Kinase Inhibitor Protein (RKIP) on the Extracellular signal Regulated Kinase (ERK) signaling pathway through mathematical modeling and...

The role of MAPK pathways in the action of chemotherapeutic drugs (2002)

Boldt, Simone, Weidle, Ulrich H., Kolch, Walter

In this study we have investigated the role of mitogen-induced and stress-activated MAP kinase pathways in the cellular response to taxol, etoposide and ceramide in three different human cancer cell...

Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP (1999)

Yeung, Kam, Seitz, Thomas, Li, Shengfeng, Janosch, Petra, McFerran, Brian, Kaiser, Christian, ...

Raf-1 phosphorylates and activates MEK-1, a kinase that activates the extracellular signal regulated kinases (ERK). This kinase cascade controls the proliferation and differentiation of different...

Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP

Yeung, Kam, Seitz, Thomas, Li, Shengfeng, Janosch, Petra, McFerran, Brian, Kaiser, Christian, ...

Raf-1 phosphorylates and activates MEK-1, a kinase that activates the extracellular signal regulated kinases (ERK). This kinase cascade controls the proliferation and differentiation of different...

Mechanism of Suppression of the Raf/MEK/Extracellular Signal-Regulated Kinase Pathway by the Raf Kinase Inhibitor Protein

Yeung, Kam, Janosch, Petra, McFerran, Brian, Rose, David W., Mischak, Harald, Sedivy, John M., ...

We have recently identified the Raf kinase inhibitor protein (RKIP) as a physiological endogenous inhibitor of the Raf-1/MEK/extracellular signal-regulated kinase (ERK) pathway. RKIP interfered with...

Raf Kinase Inhibitor Protein Interacts with NF-κB-Inducing Kinase and TAK1 and Inhibits NF-κB Activation

Yeung, Kam C., Rose, David W., Dhillon, Amardeep S., Yaros, Diane, Gustafsson, Marcus, Chatterjee, Devasis, ...

The Raf kinase inhibitor protein (RKIP) acts as a negative regulator of the mitogen-activated protein (MAP) kinase (MAPK) cascade initiated by Raf-1. RKIP inhibits the phosphorylation of...

Activation of the Epstein-Barr Virus Transcription Factor BZLF1 by 12-O-Tetradecanoylphorbol-13-Acetate-Induced Phosphorylation

Baumann, Matthias, Mischak, Harald, Dammeier, Sascha, Kolch, Walter, Gires, Olivier, Pich, Dagmar, ...

BZLF1 is a member of the extended AP-1 family of transcription factors which binds to specific BZLF1 sequence motifs within early Epstein-Barr virus (EBV) promoters and to closely related AP-1...

Regulation of Raf-1 activation and signalling by dephosphorylation

Dhillon, Amardeep S., Meikle, Sharon, Yazici, Zihni, Eulitz, Manfred, Kolch, Walter

The Raf-1 kinase is regulated by phosphorylation, and Ser259 has been identified as an inhibitory phosphorylation site. Here we show that the dephosphorylation of Ser259 is an essential part of the...

Cyclic AMP-Dependent Kinase Regulates Raf-1 Kinase Mainly by Phosphorylation of Serine 259

Dhillon, Amardeep S., Pollock, Claire, Steen, Helge, Shaw, Peter E., Mischak, Harald, Kolch, Walter

The Raf-1 kinase activates the ERK (extracellular-signal-regulated kinase) pathway. The cyclic AMP (cAMP)-dependent protein kinase (PKA) can inhibit Raf-1 by direct phosphorylation. We have mapped...

A Raf-1 Mutant That Dissociates MEK/Extracellular Signal-Regulated Kinase Activation from Malignant Transformation and Differentiation but Not Proliferation

Dhillon, Amardeep S., Meikle, Sharon, Peyssonnaux, Carole, Grindlay, Joan, Kaiser, Christian, Steen, Helge, ...

It is widely thought that the biological outcomes of Raf-1 activation are solely attributable to the activation of the MEK/extracellular signal-regulated kinase (ERK) pathway. However, an increasing...

Regulation and Role of Raf-1/B-Raf Heterodimerization†

Rushworth, Linda K., Hindley, Alison D., O'Neill, Eric, Kolch, Walter

The Ras-Raf-MEK-extracellular signal-regulated kinase (ERK) pathway participates in the control of many fundamental cellular processes including proliferation, survival, and differentiation. The...

Computational modelling of the receptor-tyrosine-kinase-activated MAPK pathway

Orton, Richard J., Sturm, Oliver E., Vyshemirsky, Vladislav, Calder, Muffy, Gilbert, David R., Kolch, Walter

The MAPK (mitogen-activated protein kinase) pathway is one of the most important and intensively studied signalling pathways. It is at the heart of a molecular-signalling network that governs the...

Mechanism of Suppression of the Raf/MEK/Extracellular Signal-Regulated Kinase Pathway by the Raf Kinase Inhibitor Protein

Yeung, Kam, Janosch, Petra, McFerran, Brian, Rose, David W., Mischak, Harald, Sedivy, John M., ...

We have recently identified the Raf kinase inhibitor protein (RKIP) as a physiological endogenous inhibitor of the Raf-1/MEK/extracellular signal-regulated kinase (ERK) pathway. RKIP interfered with...

Raf Kinase Inhibitor Protein Interacts with NF-κB-Inducing Kinase and TAK1 and Inhibits NF-κB Activation

Yeung, Kam C., Rose, David W., Dhillon, Amardeep S., Yaros, Diane, Gustafsson, Marcus, Chatterjee, Devasis, ...

The Raf kinase inhibitor protein (RKIP) acts as a negative regulator of the mitogen-activated protein (MAP) kinase (MAPK) cascade initiated by Raf-1. RKIP inhibits the phosphorylation of...

Activation of the Epstein-Barr Virus Transcription Factor BZLF1 by 12-O-Tetradecanoylphorbol-13-Acetate-Induced Phosphorylation

Baumann, Matthias, Mischak, Harald, Dammeier, Sascha, Kolch, Walter, Gires, Olivier, Pich, Dagmar, ...

BZLF1 is a member of the extended AP-1 family of transcription factors which binds to specific BZLF1 sequence motifs within early Epstein-Barr virus (EBV) promoters and to closely related AP-1...

Regulation of Raf-1 activation and signalling by dephosphorylation

Dhillon, Amardeep S., Meikle, Sharon, Yazici, Zihni, Eulitz, Manfred, Kolch, Walter

The Raf-1 kinase is regulated by phosphorylation, and Ser259 has been identified as an inhibitory phosphorylation site. Here we show that the dephosphorylation of Ser259 is an essential part of the...

Cyclic AMP-Dependent Kinase Regulates Raf-1 Kinase Mainly by Phosphorylation of Serine 259

Dhillon, Amardeep S., Pollock, Claire, Steen, Helge, Shaw, Peter E., Mischak, Harald, Kolch, Walter

The Raf-1 kinase activates the ERK (extracellular-signal-regulated kinase) pathway. The cyclic AMP (cAMP)-dependent protein kinase (PKA) can inhibit Raf-1 by direct phosphorylation. We have mapped...

A Raf-1 Mutant That Dissociates MEK/Extracellular Signal-Regulated Kinase Activation from Malignant Transformation and Differentiation but Not Proliferation

Dhillon, Amardeep S., Meikle, Sharon, Peyssonnaux, Carole, Grindlay, Joan, Kaiser, Christian, Steen, Helge, ...

It is widely thought that the biological outcomes of Raf-1 activation are solely attributable to the activation of the MEK/extracellular signal-regulated kinase (ERK) pathway. However, an increasing...

Computational modelling of the receptor-tyrosine-kinase-activated MAPK pathway

Orton, Richard J., Sturm, Oliver E., Vyshemirsky, Vladislav, Calder, Muffy, Gilbert, David R., Kolch, Walter

The MAPK (mitogen-activated protein kinase) pathway is one of the most important and intensively studied signalling pathways. It is at the heart of a molecular-signalling network that governs the...

Regulation and Role of Raf-1/B-Raf Heterodimerization†

Rushworth, Linda K., Hindley, Alison D., O'Neill, Eric, Kolch, Walter

The Ras-Raf-MEK-extracellular signal-regulated kinase (ERK) pathway participates in the control of many fundamental cellular processes including proliferation, survival, and differentiation. The...

Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP

Yeung, Kam, Seitz, Thomas, Li, Shengfeng, Janosch, Petra, McFerran, Brian, Kaiser, Christian, ...

Raf-1 phosphorylates and activates MEK-1, a kinase that activates the extracellular signal regulated kinases (ERK). This kinase cascade controls the proliferation and differentiation of different...