Anthrax Toxin Entry into Polarized Epithelial Cells
Beauregard, Kathryn E., Wimer-Mackin, Susan, Collier, R. John, Lencer, Wayne I.
We examined the entry of anthrax edema toxin (EdTx) into polarized human T84 epithelial cells using cyclic AMP-regulated Cl− secretion as an index of toxin entry. EdTx is a binary A/B toxin which...
Mel, Stephanie F., Fullner, Karla Jean, Wimer-Mackin, Susan, Lencer, Wayne I., Mekalanos, John J.
Culture supernatants prepared from reactogenic strains of Vibrio cholerae cause a decrease in the transcellular epithelial resistance of T84 intestinal cells. This decrease correlates with the...
Fullner, Karla Jean, Lencer, Wayne I., Mekalanos, John J.
To study the utility of in vitro-polarized intestinal cell monolayers for modeling Vibrio cholerae-host cell interactions, we added live V. cholerae bacteria to the apical surfaces of polarized T84...
Wimer-Mackin, Susan, Holmes, Randall K., Wolf, Anne A., Lencer, Wayne I., Jobling, Michael G.
Escherichia coli type IIa heat-labile enterotoxin (LTIIa) binds in vitro with highest affinity to ganglioside GD1b. It also binds in vitro with lower affinity to several other oligosialogangliosides...
Fujinaga, Yukako, Wolf, Anne A., Rodighiero, Chiara, Wheeler, Heidi, Tsai, Billy, Allen, Larry, ...
Cholera toxin (CT) travels from the plasma membrane of intestinal cells to the endoplasmic reticulum (ER) where a portion of the A-subunit, the A1 chain, crosses the membrane into the cytosol to...
Claypool, Steven M., Dickinson, Bonny L., Wagner, Jessica S., Johansen, Finn-Eirik, Venu, Nanda, Borawski, Jason A., ...
The human MHC class I–related neonatal Fc receptor, hFcRn, mediates bidirectional transport of IgG across mucosal barriers. Here, we find that at steady state hFcRn distributes predominantly to an...
Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line
Dickinson, Bonny L., Badizadegan, Kamran, Wu, Zhen, Ahouse, Jeremy C., Zhu, Xiaoping, Simister, Neil E., ...
The MHC class I–related Fc receptor, FcRn, mediates the intestinal absorption of maternal IgG in neonatal rodents and the transplacental transport of maternal IgG in humans by receptor-mediated...
Bitonti, Alan J., Dumont, Jennifer A., Low, Susan C., Peters, Robert T., Kropp, Keith E., Palombella, Vito J., ...
Administration of therapeutic proteins by methods other than injection is limited, in part, by inefficient penetration of epithelial barriers. Therefore, unique approaches to breaching these barriers...
Massol, Ramiro H., Larsen, Jakob E., Fujinaga, Yukako, Lencer, Wayne I., Kirchhausen, Tomas
Cholera toxin (CT) and related AB5 toxins bind to glycolipids at the plasma membrane and are then transported in a retrograde manner, first to the Golgi and then to the endoplasmic reticulum (ER). In...
Zhu, Xiaoping, Peng, Junmin, Raychowdhury, Raktima, Nakajima, Atsushi, Lencer, Wayne I, Blumberg, Richard S
The heavy chain (HC) of the neonatal Fc receptor (FcRn) for IgG is non-convalently associated with beta(2)-microglobulin (beta(2)m). In beta(2)m(-/-) mice, FcRn functions are greatly impaired. We...
Feng, Yan, Jadhav, Ashutosh P, Rodighiero, Chiara, Fujinaga, Yukako, Kirchhausen, Tomas, Lencer, Wayne I
Cholera toxin (CT) follows a glycolipid-dependent entry pathway from the plasma membrane through the trans-Golgi network (TGN) to the endoplasmic reticulum (ER) where it is retro-translocated into...
Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation
Rodighiero, Chiara, Tsai, Billy, Rapoport, Tom A., Lencer, Wayne I.
Cholera toxin travels from the cell surface of affected mammalian cells to the endoplasmic reticulum (ER), where the A1 chain is released and retro-translocated across the ER membrane into the...
Neonatal Fc receptor for IgG regulates mucosal immune responses to luminal bacteria
Yoshida, Masaru, Kobayashi, Kanna, Kuo, Timothy T., Bry, Lynn, Glickman, Jonathan N., Claypool, Steven M., ...
The neonatal Fc receptor for IgG (FcRn) plays a major role in regulating host IgG levels and transporting IgG and associated antigens across polarized epithelial barriers. Selective expression of...
Anthrax Toxin Entry into Polarized Epithelial Cells
Beauregard, Kathryn E., Wimer-Mackin, Susan, Collier, R. John, Lencer, Wayne I.
We examined the entry of anthrax edema toxin (EdTx) into polarized human T84 epithelial cells using cyclic AMP-regulated Cl− secretion as an index of toxin entry. EdTx is a binary A/B toxin which...
Mel, Stephanie F., Fullner, Karla Jean, Wimer-Mackin, Susan, Lencer, Wayne I., Mekalanos, John J.
Culture supernatants prepared from reactogenic strains of Vibrio cholerae cause a decrease in the transcellular epithelial resistance of T84 intestinal cells. This decrease correlates with the...
Fullner, Karla Jean, Lencer, Wayne I., Mekalanos, John J.
To study the utility of in vitro-polarized intestinal cell monolayers for modeling Vibrio cholerae-host cell interactions, we added live V. cholerae bacteria to the apical surfaces of polarized T84...
Wimer-Mackin, Susan, Holmes, Randall K., Wolf, Anne A., Lencer, Wayne I., Jobling, Michael G.
Escherichia coli type IIa heat-labile enterotoxin (LTIIa) binds in vitro with highest affinity to ganglioside GD1b. It also binds in vitro with lower affinity to several other oligosialogangliosides...
Fujinaga, Yukako, Wolf, Anne A., Rodighiero, Chiara, Wheeler, Heidi, Tsai, Billy, Allen, Larry, ...
Cholera toxin (CT) travels from the plasma membrane of intestinal cells to the endoplasmic reticulum (ER) where a portion of the A-subunit, the A1 chain, crosses the membrane into the cytosol to...
Claypool, Steven M., Dickinson, Bonny L., Wagner, Jessica S., Johansen, Finn-Eirik, Venu, Nanda, Borawski, Jason A., ...
The human MHC class I–related neonatal Fc receptor, hFcRn, mediates bidirectional transport of IgG across mucosal barriers. Here, we find that at steady state hFcRn distributes predominantly to an...
Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line
Dickinson, Bonny L., Badizadegan, Kamran, Wu, Zhen, Ahouse, Jeremy C., Zhu, Xiaoping, Simister, Neil E., ...
The MHC class I–related Fc receptor, FcRn, mediates the intestinal absorption of maternal IgG in neonatal rodents and the transplacental transport of maternal IgG in humans by receptor-mediated...
Bitonti, Alan J., Dumont, Jennifer A., Low, Susan C., Peters, Robert T., Kropp, Keith E., Palombella, Vito J., ...
Administration of therapeutic proteins by methods other than injection is limited, in part, by inefficient penetration of epithelial barriers. Therefore, unique approaches to breaching these barriers...
Massol, Ramiro H., Larsen, Jakob E., Fujinaga, Yukako, Lencer, Wayne I., Kirchhausen, Tomas
Cholera toxin (CT) and related AB5 toxins bind to glycolipids at the plasma membrane and are then transported in a retrograde manner, first to the Golgi and then to the endoplasmic reticulum (ER). In...
Zhu, Xiaoping, Peng, Junmin, Raychowdhury, Raktima, Nakajima, Atsushi, Lencer, Wayne I, Blumberg, Richard S
The heavy chain (HC) of the neonatal Fc receptor (FcRn) for IgG is non-convalently associated with beta(2)-microglobulin (beta(2)m). In beta(2)m(-/-) mice, FcRn functions are greatly impaired. We...
Feng, Yan, Jadhav, Ashutosh P, Rodighiero, Chiara, Fujinaga, Yukako, Kirchhausen, Tomas, Lencer, Wayne I
Cholera toxin (CT) follows a glycolipid-dependent entry pathway from the plasma membrane through the trans-Golgi network (TGN) to the endoplasmic reticulum (ER) where it is retro-translocated into...
Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation
Rodighiero, Chiara, Tsai, Billy, Rapoport, Tom A., Lencer, Wayne I.
Cholera toxin travels from the cell surface of affected mammalian cells to the endoplasmic reticulum (ER), where the A1 chain is released and retro-translocated across the ER membrane into the...
Neonatal Fc receptor for IgG regulates mucosal immune responses to luminal bacteria
Yoshida, Masaru, Kobayashi, Kanna, Kuo, Timothy T., Bry, Lynn, Glickman, Jonathan N., Claypool, Steven M., ...
The neonatal Fc receptor for IgG (FcRn) plays a major role in regulating host IgG levels and transporting IgG and associated antigens across polarized epithelial barriers. Selective expression of...
Protein disulfide isomerase–like proteins play opposing roles during retrotranslocation
Forster, Michele L., Sivick, Kelsey, Park, Young-nam, Arvan, Peter, Lencer, Wayne I., Tsai, Billy
Misfolded proteins in the endoplasmic reticulum (ER) are retained in the organelle or retrotranslocated to the cytosol for proteasomal degradation. ER chaperones that guide these opposing processes...
Dickinson, Bonny L., Claypool, Steven M., D'Angelo, June A., Aiken, Martha L., Venu, Nanda, Yen, Elizabeth H., ...
The Fcγ receptor FcRn transports immunoglobulin G (IgG) so as to avoid lysosomal degradation and to carry it bidirectionally across epithelial barriers to affect mucosal immunity. Here, we identify...
Spiekermann, Gerburg M., Finn, Patricia W., Ward, E. Sally, Dumont, Jennifer, Dickinson, Bonny L., Blumberg, Richard S., ...
Mucosal secretions of the human gastrointestinal, respiratory, and genital tracts contain the immunoglobulins (Ig)G and secretory IgA (sIgA) that function together in host defense. Exactly how IgG...
Derlin-1 Facilitates the Retro-Translocation of Cholera Toxin
Bernardi, Kaleena M., Forster, Michele L., Lencer, Wayne I., Tsai, Billy
Cholera toxin (CT) intoxicates cells by using its receptor-binding B subunit (CTB) to traffic from the plasma membrane to the endoplasmic reticulum (ER). In this compartment, the catalytic A1 subunit...
Wolf, Anne A., Jobling, Michael G., Wimer-Mackin, Susan, Ferguson-Maltzman, Margaret, Madara, James L., Holmes, Randall K., ...
In polarized cells, signal transduction by cholera toxin (CT) requires apical endocytosis and retrograde transport into Golgi cisternae and perhaps ER (Lencer, W.I., C. Constable, S. Moe, M. Jobling,...
Wolf, Anne A., Jobling, Michael G., Saslowsky, David E., Kern, Eli, Drake, Kimberly R., Kenworthy, Anne K., ...
Cholera toxin (CT) moves from the plasma membrane (PM) of host cells to the endoplasmic reticulum (ER) by binding to the lipid raft ganglioside GM1. The homopentomeric B-subunit of the toxin can bind...
Dependence of antibody-mediated presentation of antigen on FcRn
Qiao, Shuo-Wang, Kobayashi, Kanna, Johansen, Finn-Eirik, Sollid, Ludvig M., Andersen, Jan Terje, Milford, Edgar, ...
The neonatal Fc receptor for IgG (FcRn) is a distant member of the MHC class I protein family. It binds IgG and albumin in a pH-dependent manner and protects these from catabolism by diverting them...
Tzaban, Salit, Massol, Ramiro H., Yen, Elizabeth, Hamman, Wendy, Frank, Scott R., Lapierre, Lynne A., ...
The Fc receptor FcRn traffics immunoglobulin G (IgG) in both directions across polarized epithelial cells that line mucosal surfaces, contributing to host defense. We show that FcRn traffics IgG from...