Wendy Roake

Application of phage display to high throughput antibody generation and characterization (2007)

Schofield, Darren J, Pope, Anthony R, Clementel, Veronica, Buckell, Jenny, Chapple, Susan DJ, Clarke, Kay F, ...

Abstract We have created a high quality phage display library containing over 10 10 human antibodies and describe its use in the generation of antibodies on an unprecedented scale. We have selected,...

Tagging Bloodmeals with Phagemids Allows Feeding of Multiple-Sample Arrays to Single Cages of Mosquitoes (Diptera: Culicidae) and the Recovery of Single Recombinant Antibody Fragment Genes from Individual Insects (2000)

Gerry F. Killeen, Brian D. Foy, Mohammed Shahabuddin, Wendy Roake, Andrew Williams, Tristan J. Vaughan, ...

A recombinant single-chain variable-region human antibody fragment (scFv) was expressed inEscherichia coli,extracted in hypertonic sucrose, mixed directly with blood and fed toAnopheles gambiaeGiles...

Application of phage display to high throughput antibody generation and characterization

Schofield, Darren J, Pope, Anthony R, Clementel, Veronica, Buckell, Jenny, Chapple, Susan DJ, Clarke, Kay F, ...

A phage display library has been constructed containing over 1010 human antibodies, allowing the large-scale generation of antibodies. Over 38,000 recombinant antibodies against 292 antigens were...