Gu, Fang, Qi, Fengxia, Anderson, Maxwell H., Shi, Wenyuan
The aim of this study was to compare a recently developed monoclonal antibody (MAb)-based salivary Streptococcus mutans detection method with various selective media using polymerase chain reaction...
Typescript.
Effect of cellular filamentation on adventurous and social gliding motility of Myxococcus xanthus
Sun, Hong, Yang, Zhaomin, Shi, Wenyuan
Filamentous bacterial cells often provide biological information that is not readily evident in normal-size cells. In this study, the effect of cellular filamentation on gliding motility of...
Chen, Li, Ma, Lili, Park, No-Hee, Shi, Wenyuan
The oral bacteria Actinomyces naeslundii and Actinomyces viscosus are known to contribute to the initiation and progression of human dental caries, especially root caries. We report that both A....
Yang, Zhaomin, Ma, Xiaoyuan, Tong, Leming, Kaplan, Heidi B., Shimkets, Lawrence J., Shi, Wenyuan
Myxococcus xanthus social (S) gliding motility has been previously reported by us to require the chemotaxis homologues encoded by the dif genes. In addition, two cell surface structures, type IV pili...
Analyses of mrp Genes during Myxococcus xanthus Development
Myxococcus xanthus is a gram-negative soil bacterium that undergoes development under starvation conditions. Our previous study identified a new genetic locus, mrp, which is required for both...
Jewett, Anahid, Hume, Wyatt R., Le, Ho, Huynh, Tri N., Han, Yiping W., Cheng, Genhong, ...
It is largely unknown why a variety of bacteria present in the oral cavity are capable of establishing themselves in the periodontal pockets of nonimmunocompromised individuals in the presence of...
Han, Yiping W., Shi, Wenyuan, Huang, George T.-J., Kinder Haake, Susan, Park, No-Hee, Kuramitsu, Howard, ...
Bacteria are causative agents of periodontal diseases. Interactions between oral bacteria and gingival epithelial cells are essential aspects of periodontal infections. Using an in vitro tissue...
Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola
Lux, Renate, Miller, James N., Park, No-Hee, Shi, Wenyuan
The ability to penetrate tissue is an important virulence factor for pathogenic spirochetes. Previous studies have recognized the role of motility in allowing pathogenic spirochetes to invade tissues...
Under starvation conditions, Myxococcus xanthus undergoes a complex developmental process which includes cellular aggregation and sporulation. A transposon insertion mutant (the Tn5-Ω280 mutant)...
A DnaK Homolog in Myxococcus xanthus Is Involved in Social Motility and Fruiting Body Formation
Yang, Zhaomin, Geng, Yongzhi, Shi, Wenyuan
Myxococcus xanthus is a gram-negative soil bacterium which exhibits a complex life cycle and social behavior. In this study, two developmental mutants of M. xanthus were isolated through Tn5...
Chemotaxis in Borrelia burgdorferi
Shi, Wenyuan, Yang, Zhaomin, Geng, Yongzhi, Wolinsky, Lawrence E., Lovett, Michael A.
Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not...
Methylation of FrzCD Defines a Discrete Step in the Developmental Program of Myxococcus xanthus
Geng, Yongzhi, Yang, Zhaomin, Downard, John, Zusman, David, Shi, Wenyuan
Myxococcus xanthus is a gram-negative soil bacterium which undergoes fruiting body formation during starvation. The frz signal transduction system has been found to play an important role in this...
Mapping of Myxococcus xanthus Social Motility dsp Mutations to the dif Genes
Lancero, Hope, Brofft, Jennifer E., Downard, John, Birren, Bruce W., Nusbaum, Chad, Naylor, Jerome, ...
Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping,...
Construction and Characterization of a cheA Mutant of Treponema denticola
Lux, Renate, Sim, Jee-Hyun, Tsai, Jon P., Shi, Wenyuan
The Treponema denticola cheA gene, encoding the central kinase of the general chemotaxis pathway, was analyzed for its role in chemotaxis and tissue penetration. The cheA gene was interrupted by...
Bellenger, Kristen, Ma, Xiaoyuan, Shi, Wenyuan, Yang, Zhaomin
In bacteria with multiple sets of chemotaxis genes, the deletion of homologous genes or even different genes in the same operon can result in disparate phenotypes. Myxococcus xanthus is a bacterium...
Mutation of luxS Affects Biofilm Formation in Streptococcus mutans
Merritt, Justin, Qi, Fengxia, Goodman, Steven D., Anderson, Maxwell H., Shi, Wenyuan
Quorum sensing is a bacterial mechanism for regulating gene expression in response to changes in population density. Many bacteria are capable of acyl-homoserine lactone-based or peptide-based...
Extracellular polysaccharides mediate pilus retraction during social motility of Myxococcus xanthus
Li, Yinuo, Sun, Hong, Ma, Xiaoyuan, Lu, Ann, Lux, Renate, Zusman, David, ...
Myxococcus xanthus is a Gram-negative bacterium with a complex life cycle that includes vegetative swarming and fruiting-body formation. Social (S)-motility (coordinated movement of large cell...
Qi, Fengxia, Merritt, Justin, Lux, Renate, Shi, Wenyuan
Many clinical isolates of Streptococcus mutans produce peptide antibiotics called mutacins. Mutacin production may play an important role in the ecology of S. mutans in dental plaque. In this study,...
Li, Yinuo, Bustamante, Víctor H., Lux, Renate, Zusman, David, Shi, Wenyuan
Myxococcus xanthus moves on solid surfaces by using two gliding motility systems, A motility for individual-cell movement and S motility for coordinated group movements. The frz genes encode...
Pelling, Andrew E., Li, Yinuo, Shi, Wenyuan, Gimzewski, James K.
Multicellular microbial communities are the predominant form of existence for microorganisms in nature. As one of the most primitive social organisms, Myxococcus xanthus has been an ideal model...
Kreth, Jens, Merritt, Justin, Shi, Wenyuan, Qi, Fengxia
It is important to ensure DNA availability when bacterial cells develop competence. Previous studies in Streptococcus pneumoniae demonstrated that the competence-stimulating peptide (CSP) induced...
Kreth, Jens, Merritt, Justin, Shi, Wenyuan, Qi, Fengxia
The human mucosal surface is colonized by the indigenous microflora, which normally maintains an ecological balance among different species. Certain environmental or biological factors, however, may...
Eckert, Randal, Qi, Fengxia, Yarbrough, Daniel K., He, Jian, Anderson, Maxwell H., Shi, Wenyuan
Currently available antimicrobials exhibit broad killing with regard to bacterial genera and species. Indiscriminate killing of microbes by these conventional antibiotics can disrupt the ecological...
Effect of cellular filamentation on adventurous and social gliding motility of Myxococcus xanthus
Sun, Hong, Yang, Zhaomin, Shi, Wenyuan
Filamentous bacterial cells often provide biological information that is not readily evident in normal-size cells. In this study, the effect of cellular filamentation on gliding motility of...
Chen, Li, Ma, Lili, Park, No-Hee, Shi, Wenyuan
The oral bacteria Actinomyces naeslundii and Actinomyces viscosus are known to contribute to the initiation and progression of human dental caries, especially root caries. We report that both A....
Yang, Zhaomin, Ma, Xiaoyuan, Tong, Leming, Kaplan, Heidi B., Shimkets, Lawrence J., Shi, Wenyuan
Myxococcus xanthus social (S) gliding motility has been previously reported by us to require the chemotaxis homologues encoded by the dif genes. In addition, two cell surface structures, type IV pili...
Analyses of mrp Genes during Myxococcus xanthus Development
Myxococcus xanthus is a gram-negative soil bacterium that undergoes development under starvation conditions. Our previous study identified a new genetic locus, mrp, which is required for both...
Jewett, Anahid, Hume, Wyatt R., Le, Ho, Huynh, Tri N., Han, Yiping W., Cheng, Genhong, ...
It is largely unknown why a variety of bacteria present in the oral cavity are capable of establishing themselves in the periodontal pockets of nonimmunocompromised individuals in the presence of...
Han, Yiping W., Shi, Wenyuan, Huang, George T.-J., Kinder Haake, Susan, Park, No-Hee, Kuramitsu, Howard, ...
Bacteria are causative agents of periodontal diseases. Interactions between oral bacteria and gingival epithelial cells are essential aspects of periodontal infections. Using an in vitro tissue...
Motility and Chemotaxis in Tissue Penetration of Oral Epithelial Cell Layers by Treponema denticola
Lux, Renate, Miller, James N., Park, No-Hee, Shi, Wenyuan
The ability to penetrate tissue is an important virulence factor for pathogenic spirochetes. Previous studies have recognized the role of motility in allowing pathogenic spirochetes to invade tissues...
Under starvation conditions, Myxococcus xanthus undergoes a complex developmental process which includes cellular aggregation and sporulation. A transposon insertion mutant (the Tn5-Ω280 mutant)...
A DnaK Homolog in Myxococcus xanthus Is Involved in Social Motility and Fruiting Body Formation
Yang, Zhaomin, Geng, Yongzhi, Shi, Wenyuan
Myxococcus xanthus is a gram-negative soil bacterium which exhibits a complex life cycle and social behavior. In this study, two developmental mutants of M. xanthus were isolated through Tn5...
Chemotaxis in Borrelia burgdorferi
Shi, Wenyuan, Yang, Zhaomin, Geng, Yongzhi, Wolinsky, Lawrence E., Lovett, Michael A.
Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not...
Methylation of FrzCD Defines a Discrete Step in the Developmental Program of Myxococcus xanthus
Geng, Yongzhi, Yang, Zhaomin, Downard, John, Zusman, David, Shi, Wenyuan
Myxococcus xanthus is a gram-negative soil bacterium which undergoes fruiting body formation during starvation. The frz signal transduction system has been found to play an important role in this...
Mapping of Myxococcus xanthus Social Motility dsp Mutations to the dif Genes
Lancero, Hope, Brofft, Jennifer E., Downard, John, Birren, Bruce W., Nusbaum, Chad, Naylor, Jerome, ...
Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping,...
Construction and Characterization of a cheA Mutant of Treponema denticola
Lux, Renate, Sim, Jee-Hyun, Tsai, Jon P., Shi, Wenyuan
The Treponema denticola cheA gene, encoding the central kinase of the general chemotaxis pathway, was analyzed for its role in chemotaxis and tissue penetration. The cheA gene was interrupted by...
Bellenger, Kristen, Ma, Xiaoyuan, Shi, Wenyuan, Yang, Zhaomin
In bacteria with multiple sets of chemotaxis genes, the deletion of homologous genes or even different genes in the same operon can result in disparate phenotypes. Myxococcus xanthus is a bacterium...
Mutation of luxS Affects Biofilm Formation in Streptococcus mutans
Merritt, Justin, Qi, Fengxia, Goodman, Steven D., Anderson, Maxwell H., Shi, Wenyuan
Quorum sensing is a bacterial mechanism for regulating gene expression in response to changes in population density. Many bacteria are capable of acyl-homoserine lactone-based or peptide-based...
Extracellular polysaccharides mediate pilus retraction during social motility of Myxococcus xanthus
Li, Yinuo, Sun, Hong, Ma, Xiaoyuan, Lu, Ann, Lux, Renate, Zusman, David, ...
Myxococcus xanthus is a Gram-negative bacterium with a complex life cycle that includes vegetative swarming and fruiting-body formation. Social (S)-motility (coordinated movement of large cell...
Qi, Fengxia, Merritt, Justin, Lux, Renate, Shi, Wenyuan
Many clinical isolates of Streptococcus mutans produce peptide antibiotics called mutacins. Mutacin production may play an important role in the ecology of S. mutans in dental plaque. In this study,...
Li, Yinuo, Bustamante, Víctor H., Lux, Renate, Zusman, David, Shi, Wenyuan
Myxococcus xanthus moves on solid surfaces by using two gliding motility systems, A motility for individual-cell movement and S motility for coordinated group movements. The frz genes encode...
Pelling, Andrew E., Li, Yinuo, Shi, Wenyuan, Gimzewski, James K.
Multicellular microbial communities are the predominant form of existence for microorganisms in nature. As one of the most primitive social organisms, Myxococcus xanthus has been an ideal model...
Kreth, Jens, Merritt, Justin, Shi, Wenyuan, Qi, Fengxia
The human mucosal surface is colonized by the indigenous microflora, which normally maintains an ecological balance among different species. Certain environmental or biological factors, however, may...
Eckert, Randal, Qi, Fengxia, Yarbrough, Daniel K., He, Jian, Anderson, Maxwell H., Shi, Wenyuan
Currently available antimicrobials exhibit broad killing with regard to bacterial genera and species. Indiscriminate killing of microbes by these conventional antibiotics can disrupt the ecological...
Targeted Killing of Streptococcus mutans by a Pheromone-Guided “Smart” Antimicrobial Peptide
Eckert, Randal , He, Jian, Yarbrough, Daniel K., Qi, Fengxia, Anderson, Maxwell H., Shi, Wenyuan
Within the repertoire of antibiotics available to a prescribing clinician, the majority affect a broad range of microorganisms, including the normal flora. The ecological disruption resulting from...
Eckert, Randal, Brady, Keith M., Greenberg, E. Peter, Qi, Fengxia, Yarbrough, Daniel K., He, Jian, ...
Pseudomonas aeruginosa is a common opportunistic human pathogen that is associated with life-threatening acute infections and chronic airway colonization during cystic fibrosis. Previously, we...
Chalmers, Natalia I., Palmer, Robert J., Du-Thumm, Laurence, Sullivan, Richard, Shi, Wenyuan, Kolenbrander, Paul E.
Oral biofilms are multispecies communities, and in their nascent stages of development, numerous bacterial species engage in interspecies interactions. Better insight into the spatial relationship...
β-d-Allose Inhibits Fruiting Body Formation and Sporulation in Myxococcus xanthus▿
Chavira, Marielena, Cao, Nga, Le, Karen, Riar, Tanveer, Moradshahi, Navid, McBride, Melinda, ...
Myxococcus xanthus, a gram-negative soil bacterium, responds to amino acid starvation by entering a process of multicellular development which culminates in the assembly of spore-filled fruiting...
Novel Synthetic Antimicrobial Peptides against Streptococcus mutans▿
He, Jian, Eckert, Randal, Pharm, Thanh, Simanian, Maurice D., Hu, Chuhong, Yarbrough, Daniel K., ...
Streptococcus mutans, a common oral pathogen and the causative agent of dental caries, has persisted and even thrived on the tooth surface despite constant removal and eradication efforts. In this...
Tong, Huichun, Chen, Wei, Shi, Wenyuan, Qi, Fengxia, Dong, Xiuzhu
We previously demonstrated that Streptococcus oligofermentans suppressed the growth of Streptococcus mutans, the primary cariogenic pathogen, by producing hydrogen peroxide (H2O2) through lactate...
Interspecies Interactions within Oral Microbial Communities
Kuramitsu, Howard K., He, Xuesong, Lux, Renate, Anderson, Maxwell H., Shi, Wenyuan
Summary: While reductionism has greatly advanced microbiology in the past 400 years, assembly of smaller pieces just could not explain the whole! Modern microbiologists are learning “system...
Palsdottir, Hildur, Remis, Jonathan P., Schaudinn, Christoph, O'Toole, Eileen, Lux, Renate, Shi, Wenyuan, ...
Despite the fact that most bacteria grow in biofilms in natural and pathogenic ecosystems, very little is known about the ultrastructure of their component cells or about the details of their...