Lubelski, Jacek, Merkerk, Ronald Van, Konings, Wil N., Driessen, Arnold J.M.
LmrCD is a lactococcal, heterodimeric multidrug transporter, which belongs to the ABC superfamily. It consists of two half-transporters, LmrC and LmrD, that are necessary and sufficient for drug...
Plantinga, Titia H., Does, Chris Van Der, Tomkiewicz, Danuta, Keulen, Geertje Van, Konings, Wil N., Driessen, Arnold J.M.
Binding-protein-dependent secondary transporters make up a unique transport protein family. They use a solute-binding protein in proton-motive-force-driven transport. Only a few systems have been...
Poolman, Bert, Doeven, Mark K., Geertsma, Eric R., Biemans‐Oldehinkel, Esther, Konings, Wil N., Rees, Douglas C.
ATP‐binding cassette (ABC) transporters are vital to any living system and are involved in the translocation of a wide variety of substances, from ions and nutrients to high molecular weight...
Konings, Wil N., Biemans‐Oldehinkel, Esther, Geertsma, Eric R., Doeven, Mark K., Poolman, Bert, Rees, Douglas C.
ATP‐binding cassette (ABC) transporters are vital to any living system and are involved in the translocation of a wide variety of substances, from ions and nutrients to high molecular weight...
Two homologous oligopeptide binding protein genes (oppA) in Lactococcus lactis MG1363 (2004)
Konings, Wil N., Kok, Jan, Hellendoorn, Michiel, Lanfermeijer, Frank C., Sanz, Yolanda, Poolman, Bert
In previous studies, it has been shown that inactivation of opp or even oppA abolishes the capacity of Lactococcus lactis to utilize oligopeptides. We now show that the opp operon has been duplicated...
Mazurkiewicz, Piotr, Driessen, Arnold J.M., Konings, Wil N.
LmrP, a proton/multidrug antiporter of Lactococcus lactis, transports a variety of cationic substrates. Previously, two membrane-embedded acidic residues, Asp142 and Glu327, have been reported to be...
Lubelski, Jacek, Mazurkiewicz, Piotr, Merkerk, Ronald Van, Konings, Wil N., Driessen, Arnold J.M.
Multidrug resistance (MDR)-type transporters mediate the active extrusion of structurally and functionally dissimilar compounds from the cells, thereby rendering cells resistant to a range of drugs....
Facilitated Drug Influx by an Energy-uncoupled Secondary Multidrug Transporter (2004)
Mazurkiewicz, Piotr, Poelarends, Gerrit J., Driessen, Arnold J.M., Konings, Wil N.
The majority of bacterial multidrug resistance transporters belong to the class of secondary transporters. LmrP is a proton/drug antiporter of Lactococcus lactis that extrudes positively charged...
Functional characterization of the Escherichia coli K-12 yiaMNO transport protein genes (2004)
Plantinga, Titia H., Does, Chris Van Der, Badia, Josefa, Aguilar, Juan, Konings, Wil N., Driessen, Arnold J.M.
The yiaMNO genes of Escherichia coli K-12 encode a binding protein-dependent secondary, or tri-partite ATP-independent periplasmic (TRAP), transporter. Since only a few members of this family have...
Insights Into ABC Transport in Archaea (2004)
Albers, Sonja-Verena, Koning, Sonja M., Konings, Wil N., Driessen, Arnold J.M.
In archaea, ATP-binding cassette (ABC) transporters play a crucial role in substrate uptake, export, and osmoregulation. Archaeal substrate-binding-protein-dependent ABC transporters are equipped...
Two homologous oligopeptide binding protein genes (oppA) in Lactococcus lactis MG1363 (2004)
Poolman, Bert, Konings, Wil N., Kok, Jan, Hellendoorn, Michiel, Lanfermeijer, Frank C., Sanz, Yolanda
In previous studies, it has been shown that inactivation of opp or even oppA abolishes the capacity of Lactococcus lactis to utilize oligopeptides. We now show that the opp operon has been duplicated...
Transporters involved in uptake of di- and tricarboxylates in Bacillus subtilis (2003)
Krom, Bastiaan P., Warner, Jessica B., Konings, Wil N., Lolkema, Juke S.
Di- and tricarboxylates found as intermediates in the tricarboxylic acid cycle can be utilized by many bacteria and serve as carbon and energy source under aerobic and anaerobic conditions. A...
Kamp, Mart Van De, Berg, Marco Van Den, Sjollema, Klaas, Bovenberg, Roel A.L., Veenhuis, Marten, ...
Penicillin biosynthesis by Penicillium chrysogenum is a compartmentalized process. The first catalytic step is mediated by δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACV synthetase), a...
Trip, Hein, Evers, Melchior E., Konings, Wil N., Driessen, Arnold J.M.
The gene encoding the amino acid permease ArlP (Aromatic and leucine Permease) was isolated from the filamentous fungus Penicillium chrysogenum after PCR using degenerated oligonucleotides based on...
Assessment of the microbody luminal pH in the filamentous fungus Penicillium chrysogenum (2002)
Breeuwer, Pieter, Abee, Tjakko, Konings, Wil N., Driessen, Arnold J.M.
The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically...
Sugar transport in (hyper)thermophilic archaea (2002)
Koning, Sonja M., Albers, Sonja-Verena, Konings, Wil N., Driessen, Arnold J.M.
Hyperthermophilic archaea show important metabolic adaptations for growth on carbohydrates under hostile conditions. For carbohydrate uptake so far only ABC-type transporters have been described that...
Konings, Wil N., Albers, Sonja-Verena, Koning, Sonja, Driessen, Arnold J.M.
The cytoplasmic membrane of bacteria and archaea determine to a large extent the composition of the cytoplasm. Since the ion and in particular the proton and/or the sodium ion electrochemical...
Sonja M. Koning, Wil N. Konings
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different carbohydrates, such as starch, maltose and trehalose. Uptake of α-glucosides is mediated by two different, binding...
Regulation of maltose transport in Saccharomyces cerevisiae (2001)
Konings, Wil N., Brondijk, T. Harma C., Poolman, Bert
Solute transport in Saccharomyces cerevisiae can be regulated through mechanisms such as trans-inhibition and/or catabolite inactivation by nitrogen or carbon sources. Studies in hybrid membranes of...
Konings, Wil N., Bolotin, Alexander, Renault, Pierre, Lanfermeijer, Frank C., Sanz, Yolanda, Poolman, Bert
The genes encoding a binding-protein-dependent ABC transporter for dipeptides (Dpp) were identified in Lactococcus lactis subsp. cremoris MG1363. Two (dppA and dppP) of the six ORFs (dppAdppPBCDF)...
Elferink, Marieke G.L., Albers, Sonja-V., Konings, Wil N., Driessen, Arnold J.M.
The extreme thermoacidophilic archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 3 and uses a variety of sugars as sole carbon and energy source. Glucose transport in this organism is...
Koning, Sonja M., Elferink, Marieke G.L., Konings, Wil N., Driessen, Arnold J.M.
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different β-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an...
Bioenergetics and solute uptake under extreme conditions (2001)
Albers, Sonja-V., Driessen, Arnold J.M., Konings, Wil N.
The ion and particularly the proton and sodium ion permeabilities of cytoplasmic membranes play crucial roles in the bioenergetics of microorganisms. The proton and sodium permeabilities of membranes...
Glutamate transporters combine transporter- and channel-like features (2001)
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
Glutamate transporters in the mammalian central nervous system have a unique position among secondary transport proteins as they exhibit glutamate-gated chloride-channel activity in addition to...
The structure of glutamate transporters shows channel-like features (2001)
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft and thus prevent neurotoxicity. The proteins belong to a large family of secondary...
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
The carboxyl-terminal membrane-spanning segment 8 of the glutamate transporter GltT of Bacillus stearothermophilus was studied by cysteine-scanning mutagenesis. 21 single cysteine mutants were...
Regulation of maltose transport in Saccharomyces cerevisiae (2001)
Konings, Wil N., Brondijk, T. Harma C., Poolman, Bert
Solute transport in Saccharomyces cerevisiae can be regulated through mechanisms such as trans-inhibition and/or catabolite inactivation by nitrogen or carbon sources. Studies in hybrid membranes of...
Renault, Pierre, Lanfermeijer, Frank C., Sanz, Yolanda, Bolotin, Alexander, Konings, Wil N., Poolman, Bert
The genes encoding a binding-protein-dependent ABC transporter for dipeptides (Dpp) were identified in Lactococcus lactis subsp. cremoris MG1363. Two (dppA and dppP) of the six ORFs (dppAdppPBCDF)...
Konings, Wil N., Lanfermeijer, Frank C., Sanz, Yolanda, Poolman, Bert
The gene (dppA) encoding the binding protein of the di-tripeptide ABC transporter of Lactococcus lactis (DppA) was cloned under the control of the nisin promoter. Amplified expression (≈200-fold...
Lactic acid bacteria: the bugs of the new millennium (2000)
Kuipers, Oscar P., Kok, Jan, Konings, Wil N., Poolman, Bert
Lactic acid bacteria (LABs) are widely used in the manufacturing of fermented food and are among the best-studied microorganisms. Detailed knowledge of a number of physiological traits has opened new...
Konings, Wil N., Detmers, Frank J.M., Lanfermeijer, Frank C., Poolman, Bert
Lactococcus lactis degrades exogenous proteins such as β-casein to peptides of 4–30 amino acids, and uses these as nitrogen sources. The binding protein or receptor (OppALl) of the oligopeptide...
Poolman, Bert, Konings, Wil N., Fang, Gang
The peptide transport protein DtpT of Lactococcus lactis was purified and reconstituted into detergent-destabilized liposomes. The kinetics and substrate specificity of the transporter in the...
Lanfermeijer, Frank C., Kunji, Edmund R.S., Picon, Antonia, Konings, Wil N., Poolman, Bert
The kinetic properties of wild-type and mutant oligopeptide binding proteins of Lactococcus lactis were determined. To observe the properties of the mutant proteins in vivo, the oppA gene was deleted...
Tampé, Robert, Jack, Ralph W., Abele, Rupert, Lanfermeijer, Frank C., Detmers, Frank J.M., Konings, Wil N., ...
The oligopeptide transport system (Opp) of Lactococcus lactis has the unique capacity to mediate the transport of peptides from 4 up to at least 18 residues. The substrate specificity of this binding...
Lolkema, Juke S., Konings, Wil N., Brisson, Alain, Boekema, Egbert J., Ubbink-Kok, Trees
The V-type Na+-ATPase of the thermophilic, anaerobic bacterium Caloramator fervidus was purified to homogeneity. The subunit compositions of the catalytic V1 and membrane-embedded V0 parts were...
Moll, Gert N., Brul, Stanley, Konings, Wil N., Driessen, Arnold J.M.
Ac-MB21-NH2 (Ac-FASLLGKALKALAKQ-NH2) and dermaseptin S3(1-16)-NH2 (ALWKNMLKGIGKLAGK-NH2) are cationic amphipathic peptides with antimicrobial activity against a broad spectrum of microorganisms...
Diversity of transport mechanisms: common structural principles (2000)
Driessen, Arnold J.M., Rosen, Barry P., Konings, Wil N.
Traditionally, prokaryotic solute transport systems are classified into major groups based on the energetic requirement of the transport process. These include the secondary transporters that are...
Sulfur Regulation of the Sulfate Transporter Genes sutA and sutB in Penicillium chrysogenum (2000)
Kamp, Mart Van De, Schuurs, Theo A., Vos, Arnold, Konings, Wil N., Driessen, Arnold J.M.
Penicillium chrysogenum uses sulfate as a source of sulfur for the biosynthesis of penicillin. Sulfate uptake and the mRNA levels of the sulfate transporter-encoding sutB and sutA genes are all...
Krom, Bastiaan P., Warner, Jessica B., Konings, Wil N., Lolkema, Juke S.
Citrate uptake in Bacillus subtilis is stimulated by a wide range of divalent metal ions. The metal ions were separated into two groups based on the expression pattern of the uptake system. The two...
Catabolite Repression and Induction of the Mg2+-Citrate Transporter CitM of Bacillus subtilis (2000)
Warner, Jessica B., Krom, Bastiaan P., Magni, Christian, Konings, Wil N., Lolkema, Juke S.
In Bacillus subtilis the citM gene encodes the Mg2+-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region,...
Brisson, Alain, Boekema, Egbert J., Lolkema, Juke S., Konings, Wil N., Ubbink-Kok, Trees
The V-type Na+-ATPase of the thermophilic, anaerobic bacterium Caloramator fervidus was purified to homogeneity. The subunit compositions of the catalytic V1 and membrane-embedded V0 parts were...
Poolman, Bert, Konings, Wil N., Lanfermeijer, Frank C., Sanz, Yolanda
The gene (dppA) encoding the binding protein of the di-tripeptide ABC transporter of Lactococcus lactis (DppA) was cloned under the control of the nisin promoter. Amplified expression (≈200-fold...
Lactic acid bacteria: the bugs of the new millennium (2000)
Kuipers, Oscar P., Kok, Jan, Konings, Wil N., Poolman, Bert
Lactic acid bacteria (LABs) are widely used in the manufacturing of fermented food and are among the best-studied microorganisms. Detailed knowledge of a number of physiological traits has opened new...
Detmers, Frank J.M., Lanfermeijer, Frank C., Konings, Wil N., Poolman, Bert
Lactococcus lactis degrades exogenous proteins such as β-casein to peptides of 4–30 amino acids, and uses these as nitrogen sources. The binding protein or receptor (OppALl) of the oligopeptide...
Poolman, Bert, Konings, Wil N., Fang, Gang
The peptide transport protein DtpT of Lactococcus lactis was purified and reconstituted into detergent-destabilized liposomes. The kinetics and substrate specificity of the transporter in the...
Kunji, Edmund R.S., Picon, Antonia, Lanfermeijer, Frank C., Konings, Wil N., Poolman, Bert
The kinetic properties of wild-type and mutant oligopeptide binding proteins of Lactococcus lactis were determined. To observe the properties of the mutant proteins in vivo, the oppA gene was deleted...
Poolman, Bert, Konings, Wil N., Tampé, Robert, Jack, Ralph W., Abele, Rupert, Lanfermeijer, Frank C., ...
The oligopeptide transport system (Opp) of Lactococcus lactis has the unique capacity to mediate the transport of peptides from 4 up to at least 18 residues. The substrate specificity of this binding...
Konings, Wil N., Poolman, Bert, Rest, Michel Van Der, Fristedt, Ulrika, Persson, Bengt L.
The proton-coupled Pho84 phosphate permease of Saccharomyces cerevisiae, overexpressed as a histidine-tagged chimera in Escherichia coli, was detergent-solubilized, purified, and reconstituted into...
Konings, Wil N., Picon, Antonia, Lanfermeijer, Frank C., Poolman, Bert
The oligopeptide transport system (Opp) of Lactococcus lactis belongs to the class of binding protein-dependent ABC-transporters. This system has the unique capacity to mediate the uptake of peptides...
Poolman, Bert, Veen, Hendrik W. Van, Putman, Monique, Konings, Wil N.
The histidine-tagged secondary multidrug transporter LmrP was overexpressed in Lactococcus lactis, using a novel protein expression system for cytotoxic proteins based on the tightly regulated,...
Poolman, Bert, Hagting, Anja, Lanfermeijer, Frank, Friesen, Robert, Fang, Gang, Konings, Wil N.
The di-tripeptide transport system (DtpT) of Lactococcus lactis was purified to apparent homogeneity by pre-extraction of crude membrane vesicles with octaethylene glycol monodecyl ether (C10E8),...
Driessen, Arnold J.M., Costa, Milton S. Da, Konings, Wil N.
Bacillus subtilis was grown at its growth temperature limits and at various temperatures in between the lower and upper growth temperature boundary. Liposomes were made of the extracted membrane...
A unique short signal sequence in membrane-anchored proteins of Archaea (1999)
Albers, Sonja-Verena, Konings, Wil N., Driessen, Arnold J.M.
Kamp, Mart Van De, Pizzinini, Enrica, Vos, Arnold, Schuurs, Theo A., Newbert, Roger W., ...
In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products...
Moll, Gert N., Akker, Emile Van Den, Hauge, Håvard H., Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., ...
Plantaricin EF and JK are both two-peptide bacteriocins produced by Lactobacillus plantarum C11. The mechanism of plantaricin EF and JK action was studied on L. plantarum 965 cells. Both plantaricins...
Albers, Sonja-V., Elferink, Marieke G.L., Charlebois, Robert L., Sensen, Christoph W., Driessen, Arnold J.M., Konings, Wil N.
The archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This...
Driessen, Arnold J.M., Grant, William D., Konings, Wil N.
The influence of pH and the salt concentration on the proton and sodium ion permeability of liposomes formed from lipids of the halophile Halobacterium salinarum and the haloalkaliphile Halorubrum...
Bacteriocins: mechanism of membrane insertion and pore formation (1999)
Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M.
Lactic acid bacteria produce several types of pore forming peptides. Class I bacteriocins are lantibiotics that contain (methyl)lanthionine residues that may form intramolecular thioether rings....
Compartmentalization and transport in β-lactam antibiotic biosynthesis by filamentous fungi (1999)
Kamp, Mart Van De, Driessen, Arnold J.M., Konings, Wil N.
A proper description of the biosynthesis of fungal β-lactam antibiotics requires detailed knowledge of the cell biology of the producing organisms. This involves a delineation of the...
Slotboom, Dirk Jan, Sobczak, Iwona, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft. The proteins belong to a large family of secondary transporters, which includes...
Magni, Christian, Mendoza, Diego De, Konings, Wil N., Lolkema, Juke S.
Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was...
Persson, Bengt L., Konings, Wil N., Poolman, Bert, Rest, Michel Van Der, Fristedt, Ulrika
The proton-coupled Pho84 phosphate permease of Saccharomyces cerevisiae, overexpressed as a histidine-tagged chimera in Escherichia coli, was detergent-solubilized, purified, and reconstituted into...
Poolman, Bert, Konings, Wil N., Picon, Antonia, Lanfermeijer, Frank C.
The oligopeptide transport system (Opp) of Lactococcus lactis belongs to the class of binding protein-dependent ABC-transporters. This system has the unique capacity to mediate the uptake of peptides...
Konings, Wil N., Poolman, Bert, Veen, Hendrik W. Van, Putman, Monique
The histidine-tagged secondary multidrug transporter LmrP was overexpressed in Lactococcus lactis, using a novel protein expression system for cytotoxic proteins based on the tightly regulated,...
Lanfermeijer, Frank, Friesen, Robert, Fang, Gang, Hagting, Anja, Poolman, Bert, Konings, Wil N.
The di-tripeptide transport system (DtpT) of Lactococcus lactis was purified to apparent homogeneity by pre-extraction of crude membrane vesicles with octaethylene glycol monodecyl ether (C10E8),...
Lanfermeijer, Frank C., Kunji, Edmund R.S., Detmers, Frank J.M., Poolman, Bert, Konings, Wil N.
To obtain amino acids for growth, Lactococcus lactis uses a proteolytic system to degrade exogenous proteins such as caseins. The extracellular cell wall-attached proteinase PrtP and the oligopeptide...
Bacterial solute uptake and efflux systems (1998)
Poolman, Bert, Lolkema, Juke S., Konings, Wil N.
The recent discovery of binding protein dependent secondary transporters and the ever-growing family of membrane potential generating secondary transporters emphasize the diversity of transport...
Amino acid transport in Lactobacillus helveticus (1998)
Poolman, Bert, Kunji, Edmund R.S., Nakajima, Hadjime, Konings, Wil N.
Amino acid transport in Lactobacillus helveticus was analyzed. Strain specificity of amino acid transport was speculated between L. helveticus NCDO2712 and SBT2171. Glucose energized L. helveticus...
Physiological Response of Lactobacillus plantarum to Salt and Nonelectrolyte Stress (1998)
Steeg, Pieter F. Ter, Tjan, Frans S.B., Glaasker, Erwin, Konings, Wil N., Poolman, Bert
In this report, we compared the effects on the growth of Lactobacillus plantarum of raising the medium molarity by high concentrations of KCl or NaCl and iso-osmotic concentrations of nonionic...
Konings, Wil N., Glaasker, Erwin, Poolman, Bert
The accumulation of quaternary ammonium compounds in Lactobacillus plantarum is mediated via a single transport system with a high affinity for glycine betaine (apparent Km of 18 μM) and carnitine...
Stingl, Kerstin, Vries, Yne De, Pluim, Dick, Brondijk, T. Harma C., Poolman, Bert, ...
The maltose transporter of Saccharomyces cerevisiae is subject to rapid, irreversible inactivation in the presence of glucose. Loss of transport function was paralleled by a decrease in amount of...
Reconstruction of the proteolytic pathway for use of β-casein by Lactococcus lactis (1998)
Bruins, Andries P., Jeronimus-Stratingh, C. Margot, Fang, Gang, Kunji, Edmund R.S., Poolman, Bert, Konings, Wil N.
Amino acid auxotrophous bacteria such as Lactococcus lactis use proteins as a source of amino acids. For this process, they possess a complex proteolytic system to degrade the protein(s) and to...
Hauge, Håvard Hildeng, Mantzilas, Dimitris, Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M., Eijsink, Vincent G.H., ...
Production of bacteriocins by lactic acid bacteria is in some cases regulated by a quorum sensing mechanism that involves a secreted bacteriocin-like peptide pheromone. In the case of Lactobacillus...
Mechanistic Properties of the Two-Component Bacteriocin Lactococcin G (1998)
Moll, Gert, Hildeng-Hauge, Håvard, Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., Driessen, Arnold J.M.
Lactococcin G is a bacteriocin whose activity depends on the complementary action of two peptides, termed α and β. Biologically active, synthetic lactococcin G was used to study the mode of action...
The Lantibiotic Nisin Induces Transmembrane Movement of a Fluorescent Phospholipid (1998)
Moll, Gert N., Konings, Wil N., Driessen, Arnold J.M.
Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid...
Driessen, Arnold J.M., Zillig, Wolfram, Konings, Wil N.
Picrophilus oshimae is an extremely acidophilic, thermophilic archae on that grows optimally at 60°C and at pH 0.7. It is an obligatory acidophile that does not grow at pH values above 4.0. The...
The essence of being extremophilic: the role of the unique archaeal membrane lipids (1998)
Driessen, Arnold J.M., Konings, Wil N.
In extreme environments, mainly Archaea are encountered. The archaeal cytoplasmic membrane contains unique ether lipids that cannot easily be degraded, are temperature- and mechanically resistant,...
Lanfermeijer, Frank C., Kunji, Edmund R.S., Detmers, Frank J.M., Poolman, Bert, Konings, Wil N.
To obtain amino acids for growth, Lactococcus lactis uses a proteolytic system to degrade exogenous proteins such as caseins. The extracellular cell wall-attached proteinase PrtP and the oligopeptide...
Bacterial solute uptake and efflux systems (1998)
Konings, Wil N., Poolman, Bert, Lolkema, Juke S.
The recent discovery of binding protein dependent secondary transporters and the ever-growing family of membrane potential generating secondary transporters emphasize the diversity of transport...
Amino acid transport in Lactobacillus helveticus (1998)
Kunji, Edmund R.S., Nakajima, Hadjime, Poolman, Bert, Konings, Wil N.
Amino acid transport in Lactobacillus helveticus was analyzed. Strain specificity of amino acid transport was speculated between L. helveticus NCDO2712 and SBT2171. Glucose energized L. helveticus...
Physiological Response of Lactobacillus plantarum to Salt and Nonelectrolyte Stress (1998)
Steeg, Pieter F. Ter, Tjan, Frans S.B., Glaasker, Erwin, Konings, Wil N., Poolman, Bert
In this report, we compared the effects on the growth of Lactobacillus plantarum of raising the medium molarity by high concentrations of KCl or NaCl and iso-osmotic concentrations of nonionic...
Poolman, Bert, Konings, Wil N., Glaasker, Erwin
The accumulation of quaternary ammonium compounds in Lactobacillus plantarum is mediated via a single transport system with a high affinity for glycine betaine (apparent Km of 18 μM) and carnitine...
Pluim, Dick, Brondijk, T. Harma C., Vries, Yne De, Stingl, Kerstin, Poolman, Bert, ...
The maltose transporter of Saccharomyces cerevisiae is subject to rapid, irreversible inactivation in the presence of glucose. Loss of transport function was paralleled by a decrease in amount of...
Reconstruction of the proteolytic pathway for use of β-casein by Lactococcus lactis (1998)
Bruins, Andries P., Jeronimus-Stratingh, C. Margot, Fang, Gang, Kunji, Edmund R.S., Poolman, Bert, Konings, Wil N.
Amino acid auxotrophous bacteria such as Lactococcus lactis use proteins as a source of amino acids. For this process, they possess a complex proteolytic system to degrade the protein(s) and to...
Kunji, Edmund R.S., Hagting, Anja, Nakajima, Hajime, Poolman, Bert, Konings, Wil N.
The gene encoding the di- and tripeptide transport protein (DtpT) of Lactobacillus helveticus (DtpTLH) was cloned with the aid of the inverse PCR technique and used to complement the dipeptide...
Membrane Topology of the Di- and Tripeptide Transport Protein of Lactococcus lactis (1997)
Poolman, Bert, Velde, Joeke V.d., Hagting, Anja, Konings, Wil N.
Transport of hydrophilic di- and tripeptides into Lactococcus lactis is mediated by a proton motive force-driven peptide transport protein (DtpT) that shares similarity with eukaryotic peptide...
Adaptation of Microorganisms and Their Transport Systems to High Temperatures (1997)
Konings, Wil N., Poolman, Bert, Tolner, Berend
Growth of Bacteria and Archaea has been observed at temperatures up to 95 and 110°C, respectively. These thermophiles are adapted to environments of high temperature by changes in the membrane lipid...
Fang, Gang, Streutker, Marion R., Hasemeier, Britta, Knol, Jan, Hagting, Anja, Poolman, Bert, ...
Transport of hydrophilic dipeptides and tripeptides into Lactococcus lactis is mediated by a proton-motive-force-driven peptide-transport protein (DtpT) that shares similarity to eukaryotic peptide...
Mechanisms of multidrug transporters (1997)
Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert, Veen, Hendrik W. Van, Bolhuis, Henk
Drug resistance, mediated by various mechanisms, plays a crucial role in the failure of the drug-based treatment of various infectious diseases. As a result, these infectious diseases re-emerge...
Slow fusion of liposomes composed of membrane-spanning lipids (1997)
Elferink, Marieke G.L., Breemen, Jan Van, Konings, Wil N., Driessen, Arnold J.M., Wilschut, Jan
The fusion characteristics of large unilamellar liposomes composed of bipolar tetraether lipids extracted from the thermophilic archaeon Sulfolobus acidocaldarius, was investigated. These lipids span...
Role of Transmembrane pH Gradient and Membrane Binding in Nisin Pore Formation (1997)
Moll, Gert N., Clark, Jonathan, Chan, Weng C., Bycroft, Barrie W., Roberts, Gordon C.K., Konings, Wil N., ...
Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane...
An Na+-Pumping V1V0-ATPase Complex in the Thermophilic Bacterium Clostridium fervidus (1997)
Höner Zu Bentrup, Kerstin, Ubbink-Kok, Trees, Lolkema, Juke S., Konings, Wil N.
Energy transduction in the anaerobic, thermophilic bacterium Clostridium fervidus relies exclusively on Na+ as the coupling ion. The Na+ ion gradient across the membrane is generated by a...
Konings, Wil N., Poolman, Bert, Kunji, Edmund R.S., Hagting, Anja, Nakajima, Hajime
The gene encoding the di- and tripeptide transport protein (DtpT) of Lactobacillus helveticus (DtpTLH) was cloned with the aid of the inverse PCR technique and used to complement the dipeptide...
Membrane Topology of the Di- and Tripeptide Transport Protein of Lactococcus lactis (1997)
Konings, Wil N., Poolman, Bert, Velde, Joeke V.d., Hagting, Anja
Transport of hydrophilic di- and tripeptides into Lactococcus lactis is mediated by a proton motive force-driven peptide transport protein (DtpT) that shares similarity with eukaryotic peptide...
Adaptation of Microorganisms and Their Transport Systems to High Temperatures (1997)
Konings, Wil N., Poolman, Bert, Tolner, Berend
Growth of Bacteria and Archaea has been observed at temperatures up to 95 and 110°C, respectively. These thermophiles are adapted to environments of high temperature by changes in the membrane lipid...
Hasemeier, Britta, Knol, Jan, Hagting, Anja, Streutker, Marion R., Fang, Gang, Poolman, Bert, ...
Transport of hydrophilic dipeptides and tripeptides into Lactococcus lactis is mediated by a proton-motive-force-driven peptide-transport protein (DtpT) that shares similarity to eukaryotic peptide...
Mechanisms of multidrug transporters (1997)
Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert, Veen, Hendrik W. Van, Bolhuis, Henk
Drug resistance, mediated by various mechanisms, plays a crucial role in the failure of the drug-based treatment of various infectious diseases. As a result, these infectious diseases re-emerge...
The proteolytic systems of lactic acid bacteria (1996)
Poolman, Bert, Hagting, Anja, Mierau, Igor, Kunji, Edmund R.S., Konings, Wil N.
Proteolysis in dairy lactic acid bacteria has been studied in great detail by genetic, biochemical and ultrastructural methods. From these studies the picture emerges that the proteolytic systems of...
Konings, Wil N., Driessen, Arnold J.M., Poolman, Bert, Molenaar, Douwe, Veen, Hendrik W. Van, Bolhuis, Henk
Lactococcus lactis possesses an ATP-dependent drug extrusion system which shares functional properties with the mammalian multidrug resistance (MDR) transporter P-glycoprotein. One of the intriguing...
Osmotic Regulation of Intracellular Solute Pools in Lactobacillus plantarum (1996)
Poolman, Bert, Konings, Wil N., Glaasker, Erwin
Bacteria respond to changes in medium osmolarity by varying the concentrations of specific solutes in order to maintain constant turgor pressure. The cytoplasmic pools of K+, proline, glutamate,...
Poolman, Bert, Haandrikman, Alfred J., Hellendoorn, Michiel A., Leenhouts, Kees J., Kunji, Edmund R.S., Mierau, Igor, ...
To examine the contribution of peptidases to the growth of Lactococcus lactis in milk, 16 single- and multiple-deletion mutants were constructed. In successive rounds of chromosomal gene replacement...
Driessen, Arnold J.M., Poolman, Bert, Putman, Monique, Brands, Jan Roel, Veen, Hendrik W. Van, Bolhuis, Henk, ...
The gene encoding the secondary multidrug transporter LmrP of Lactococcus lactis was heterologously expressed in Escherichia coli. The energetics and mechanism of drug extrusion mediated by LmrP were...
Poolman, Bert, Konings, Wil N., Glaasker, Erwin
Bacteria respond to changes in medium osmolarity by varying the concentrations of specific solutes in order to maintain constant turgor. The primary response of Lactobacillus plantarum to an osmotic...
Fate of peptides in peptidase mutants of Lactococcus lactis (1996)
Venema, Gerard, Konings, Wil N., Poolman, Bert, Mierau, Igor, Kunji, Edmund R.S., Kok, Jan
The utilization of exogenous peptides was studied in mutants of Lactococcus lactis in which combinations of the peptidase genes pepN, pepC, pepO, pepX and pepT were deleted. Multiple mutants lacking...
Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1 (1996)
Poolman, Bert, Kok, Jan, Oussenko, Irina, Bolhuis, Henk, Venema, Koen, Veen, Hendrik W. Van, ...
Resistance of Lactococcus lactis to cytotoxic compounds shares features with the multidrug resistance phenotype of mammalian tumor cells. Here, we report the gene cloning and functional...
Robillard, George T., Konings, Wil N., Tolner, Berend, Henstra, Sytse A.
A mannitol phosphotransferase system (PTS) was identified in Bacillus stearothermophilus by in vitro complementation with Escherichia coli EI, HPr, and IIAMtl. Degenerate primers based on regions of...
Membrane composition and ion-permeability in extremophiles (1996)
Driessen, Arnold J.M., Konings, Wil N.
Protons and sodium ions are the only used coupling ions in energy transduction in Bacteria and Archaea. At their growth temperature, the permeability of the cytoplasmic membrane of thermophilic...
Jacobs, Mariken H.J., Heide, Tiemen Van Der, Driessen, Arnold J.M., Konings, Wil N.
Growth of a glutamate transport-deficient mutant of Rhodobacter sphaeroides on glutamate as sole carbon and nitrogen source can be restored by the addition of millimolar amounts of Na+. Uptake of...
Bactericidal Mode of Action of Plantaricin C (1996)
González, Beatriz, Glaasker, Erwin, Kunji, Edmund R.S., Driessen, Arnold J.M., Suárez, Juan E., Konings, Wil N.
Plantaricin C is a bacteriocin produced by Lactobacillus plantarum LL441 that kills sensitive cells by acting on the cytoplasmic membrane. In contrast to its lack of impact on immune cells,...
Sulfate Transport in Penicillium chrysogenum Plasma Membranes (1996)
Hillenga, Dirk J., Versantvoort, Hanneke J.M., Driessen, Arnold J.M., Konings, Wil N.
Transport studies with Penicillium chrysogenum plasma membranes fused with cytochrome c oxidase liposomes demonstrate that sulfate uptake is driven by the transmembrane pH gradient and not by the...
Basic Amino Acid Transport in Plasma Membrane Vesicles of Penicillium chrysogenum (1996)
Hillenga, Dirk J., Versantvoort, Hanneke J.M., Driessen, Arnold J.M., Konings, Wil N.
The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart...
Sodium-Coupled Energy Transduction in the Newly Isolated Thermoalkaliphilic Strain LBS3 (1996)
Prowe, Steffen G., Driessen, Arnold J.M., Antranikian, Garabed, Konings, Wil N.
Strain LBS3 is a novel anaerobic thermoalkaliphilic bacterium that grows optimally at pH 9.5 and 50°C. Since a high concentration of Na+ ions is required for growth, we have analyzed the primary...
Lactococcin G Is a Potassium Ion-Conducting, Two-Component Bacteriocin (1996)
Moll, Gert, Ubbink-Kok, Trees, Hildeng-Hauge, Håvard, Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., ...
Lactococcin G is a novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides, termed α and β. Peptide synthesis of the α and β peptides yielded biologically...
Mechanism of lantibiotic-induced pore-formation (1996)
Moll, Gert N., Roberts, Gordon C.K., Konings, Wil N., Driessen, Arnold J.M.
Nisin and other lantibiotics have a bacteriocidal effect against Gram-positive bacteria, and also inhibit the outgrowth of bacterial spores. The bacteriocidal effect appears to be due to the...
Gaillard, Isabelle, Slotboom, Dirk-Jan, Knol, Jan, Lolkema, Juke S., Konings, Wil N.
An affinity tag consisting of six adjacent histidine residues followed by an enterokinase cleavage site was genetically engineered at the N-terminus of the glutamate transport protein GltT of the...
Slotboom, Dirk Jan, Lolkema, Juke S., Konings, Wil N.
Secondary glutamate transporters in neuronal and glial cells in the mammalian central nervous system remove the excitatory neurotransmitter glutamate from the synaptic cleft and prevent the...
Proton Motive Force Generation by Citrolactic Fermentation in Leuconostoc mesenteroides (1996)
Marty-Teysset, Claire, Posthuma, Clara, Lolkema, Juke S., Schmitt, Philippe, Divies, Charles, Konings, Wil N.
In Leuconostoc mesenteroides subsp. mesenteroides 19D, citrate is transported by a secondary citrate carrier (CitP). Previous studies of the kinetics and mechanism of CitP performed in membrane...
Marty-Teysset, Claire, Lolkema, Juke S., Schmitt, Philippe, Diviès, Charles, Konings, Wil N.
Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism. The pathway consists of four steps: (i)...
Boorsma, André, Lolkema, Juke S., Konings, Wil N.
Citrate uptake in Bacillus subtilis is mediated by a secondary transporter that transports the complex of citrate and divalent metal ions. The gene coding for the transporter termed CitM was cloned,...
Robillard, George T., Konings, Wil N., Tolner, Berend, Henstra, Sytse A.
A mannitol phosphotransferase system (PTS) was identified in Bacillus stearothermophilus by in vitro complementation with Escherichia coli EI, HPr, and IIAMtl. Degenerate primers based on regions of...
The proteolytic systems of lactic acid bacteria (1996)
Hagting, Anja, Mierau, Igor, Kunji, Edmund R.S., Poolman, Bert, Konings, Wil N.
Proteolysis in dairy lactic acid bacteria has been studied in great detail by genetic, biochemical and ultrastructural methods. From these studies the picture emerges that the proteolytic systems of...
Poolman, Bert, Molenaar, Douwe, Veen, Hendrik W. Van, Bolhuis, Henk, Driessen, Arnold J.M., Konings, Wil N.
Lactococcus lactis possesses an ATP-dependent drug extrusion system which shares functional properties with the mammalian multidrug resistance (MDR) transporter P-glycoprotein. One of the intriguing...
Osmotic Regulation of Intracellular Solute Pools in Lactobacillus plantarum (1996)
Poolman, Bert, Konings, Wil N., Glaasker, Erwin
Bacteria respond to changes in medium osmolarity by varying the concentrations of specific solutes in order to maintain constant turgor pressure. The cytoplasmic pools of K+, proline, glutamate,...
Hellendoorn, Michiel A., Leenhouts, Kees J., Kunji, Edmund R.S., Mierau, Igor, Haandrikman, Alfred J., Poolman, Bert, ...
To examine the contribution of peptidases to the growth of Lactococcus lactis in milk, 16 single- and multiple-deletion mutants were constructed. In successive rounds of chromosomal gene replacement...
Driessen, Arnold J.M., Poolman, Bert, Putman, Monique, Brands, Jan Roel, Veen, Hendrik W. Van, Bolhuis, Henk, ...
The gene encoding the secondary multidrug transporter LmrP of Lactococcus lactis was heterologously expressed in Escherichia coli. The energetics and mechanism of drug extrusion mediated by LmrP were...
Poolman, Bert, Konings, Wil N., Glaasker, Erwin
Bacteria respond to changes in medium osmolarity by varying the concentrations of specific solutes in order to maintain constant turgor. The primary response of Lactobacillus plantarum to an osmotic...
Fate of peptides in peptidase mutants of Lactococcus lactis (1996)
Venema, Gerard, Konings, Wil N., Poolman, Bert, Mierau, Igor, Kunji, Edmund R.S., Kok, Jan
The utilization of exogenous peptides was studied in mutants of Lactococcus lactis in which combinations of the peptidase genes pepN, pepC, pepO, pepX and pepT were deleted. Multiple mutants lacking...
Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1 (1996)
Driessen, Arnold J.M., Poolman, Bert, Kok, Jan, Oussenko, Irina, Bolhuis, Henk, Venema, Koen, ...
Resistance of Lactococcus lactis to cytotoxic compounds shares features with the multidrug resistance phenotype of mammalian tumor cells. Here, we report the gene cloning and functional...
Glaasker, Erwin, Konings, Wil N., Poolman, Bert
lntracellular pH in bacteria can be measured efficiently between internal pH values of 6.5 and 8.5 with the fluorescent pH indicator 2’,7’-bis-(2-carboxyethyl)-5[and-6]-carboxyfluorescein...
The generation of metabolic energy by solute transport (1995)
Poolman, Bert, Lolkema, Juke S., Konings, Wil N.
Secondary metabolic-energy-generating systems generate a proton motive force (pmf) or a sodium ion motive force (smf) by a process that involves the action of secondary transporters. The...
Desmazeaud, Michel, Konings, Wil N., Richard, Jean, Hagting, Anja, Kunji, Edmund R.S., Foucaud, Catherine, ...
A proton motive force-driven di-tripeptide carrier protein (DtpT) and an ATP-dependent oligopeptide transport system (Opp) have been described for Lactococcus lactis MG1363. Using genetically...
Poolman, Bert, Ubbink-Kok, Trees, Tolner, Berend, Konings, Wil N.
Transport of acidic amino acids in Bacillus subtilis is an electrogenic process in which L-glutamate or L-aspartate is symported with at least two protons. This is shown by studies of transport in...
The Lactococcal lmrP Gene Encodes a Proton Motive Force-dependent Drug Transporter (1995)
Driessen, Arnold J.M., Poolman, Bert, Veen, Hendrik W. Van, Poelarends, Gerrit, Bolhuis, Henk, Konings, Wil N.
To genetically dissect the drug extrusion systems of Lactococcus lactis, a chromosomal DNA library was made in Escherichia coli and recombinant strains were selected for resistance to high...
Haandrikman, Alfred J., Juillard, Vincent, Vries, Corry J. De, Hagting, Anja, Kunji, Edmund R.S., Poolman, Bert, ...
In the proteolytic pathway of Lactococcus lactis, milk proteins (caseins) are hydrolyzed extracellularly to oligopeptides by the proteinase (PrtP). The fate of these peptides, i.e. extracellular...
The Plasma Membrane of Saccharomyces cerevisiae: Structure, Function, and Biogenesis (1995)
Poolman, Bert, Anraku, Yasuhiro, Nakano, Akihiko, Kamminga, Anne H., Konings, Wil N.
Konings, Wil N., Poolman, Bert, Ubbink-Kok, Trees, Tolner, Berend
L-Glutamate transport by the H+-glutamate and Na+-glutamate symport proteins of Escherichia coil K-12 (GltPEc and GltSEc, respectively) and the Na+-H+-glutamate symport proteins of Bacillus...
Mechanistic Studies of Lantibiotic-Induced Permeabilization of Phospholipid Vesicles (1995)
Driessen, Arnold J.M., Kuiper, Wieny, Kamp, Mart Van De, Sahl, Hans-Georg, Konings, Ruud N.H., ...
Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes...
Thermostability of respiratory terminal oxidases in the lipid environment (1995)
Elferink, Marieke G.L., Bosmal, Tjibbe, Lolkema, Juke S., Gleiszner, Michael, Driessen, Arnold J.M., Konings, Wil N.
The effect of the lipid environment on the thermostability of three respiratory terminal oxidases was determined. Cytochrome-c oxidase from beef heart and Bacillus stearothermophilus were used as...
Mutacin II, a Bactericidal Lantibiotic from Streptococcus mutans (1995)
Chikindas, Michael L., Novák, Jan, Driessen, Arnold J.M., Konings, Wil N., Schilling, Kurt M., Caufield, Page W.
Mutacin II is a lantibiotic that is produced by group II Streptococcus mutans. It inhibits the growth of other streptococci as well as many other gram-positive microorganisms by a hitherto unknown...
Hillenga, Dirk J., Versantvoort, Hanneke J.M., Molen, Siep Van Der, Driessen, Arnold J.M., Konings, Wil N.
Penicillium chrysogenum utilizes phenylacetic acid as a side chain precursor in penicillin G biosynthesis. During industrial production of penicillin G, phenylacetic acid is fed in small amounts to...
Jacobs, Mariken H.J., Driessen, Arnold J.M., Konings, Wil N.
The mechanism of L-glutamate uptake was studied in Rhodobacter sphaeroides. Uptake of L-glutamate is mediated by a high-affinity (Kt of 1.2 µM), shock-sensitive transport system that is inhibited by...
Jacobs, Mariken H.J., Heide, Tiemen Van Der, Tolner, Berend, Driessen, Arnold J.M., Konings, Wil N.
Rhodobacter sphaeroides is chemotactic to glutamate and most other amino acids. In Escherichia coli, chemotaxis involves a membrane-bound sensor that either binds the amino acid directly or interacts...
Ubbink-Kok, Trees, Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N.
Protons and sodium ions are the most commonly used coupling ions in energy transduction in bacteria and archaea. At their growth temperature, the permeability of the cytoplasmic membrane of...
Marty-Teysset, Claire, Lolkema, Juke S., Schmitt, Philippe, Divies, Charles, Konings, Wil N.
Citrate uptake in Leuconostoc mesenteroides subsp. mesenteroides 19D is catalyzed by a secondary citrate carrier (CitP). The kinetics and mechanism of CitP were investigated in membrane vesicles of...
Enzyme Basis for pH Regulation of Citrate and Pyruvate Metabolism by Leuconostoc oenos (1995)
Ramos, Ana, Lolkema, Juke S., Konings, Wil N., Santos, Helena
Citrate and pyruvate metabolism by nongrowing cells of Leuconostoc oenos was investigated. 13C nuclear magnetic resonance (NMR) spectroscopy was used to elucidate the pathway of citrate breakdown and...
The generation of metabolic energy by solute transport (1995)
Poolman, Bert, Lolkema, Juke S., Konings, Wil N.
Secondary metabolic-energy-generating systems generate a proton motive force (pmf) or a sodium ion motive force (smf) by a process that involves the action of secondary transporters. The...
Hagting, Anja, Kunji, Edmund R.S., Foucaud, Catherine, Richard, Jean, Konings, Wil N., Desmazeaud, Michel, ...
A proton motive force-driven di-tripeptide carrier protein (DtpT) and an ATP-dependent oligopeptide transport system (Opp) have been described for Lactococcus lactis MG1363. Using genetically...
Konings, Wil N., Poolman, Bert, Ubbink-Kok, Trees, Tolner, Berend
Transport of acidic amino acids in Bacillus subtilis is an electrogenic process in which L-glutamate or L-aspartate is symported with at least two protons. This is shown by studies of transport in...
The Lactococcal lmrP Gene Encodes a Proton Motive Force-dependent Drug Transporter (1995)
Veen, Hendrik W. Van, Poelarends, Gerrit, Bolhuis, Henk, Poolman, Bert, Driessen, Arnold J.M., Konings, Wil N.
To genetically dissect the drug extrusion systems of Lactococcus lactis, a chromosomal DNA library was made in Escherichia coli and recombinant strains were selected for resistance to high...
Juillard, Vincent, Vries, Corry J. De, Hagting, Anja, Kunji, Edmund R.S., Haandrikman, Alfred J., Poolman, Bert, ...
In the proteolytic pathway of Lactococcus lactis, milk proteins (caseins) are hydrolyzed extracellularly to oligopeptides by the proteinase (PrtP). The fate of these peptides, i.e. extracellular...
The Plasma Membrane of Saccharomyces cerevisiae: Structure, Function, and Biogenesis (1995)
Kamminga, Anne H., Nakano, Akihiko, Anraku, Yasuhiro, Poolman, Bert, Konings, Wil N.
Konings, Wil N., Poolman, Bert, Ubbink-Kok, Trees, Tolner, Berend
L-Glutamate transport by the H+-glutamate and Na+-glutamate symport proteins of Escherichia coil K-12 (GltPEc and GltSEc, respectively) and the Na+-H+-glutamate symport proteins of Bacillus...
Solute transport and energy transduction in bacteria (1994)
Veen, Hendrik W. Van, Poolman, Bert, Konings, Wil N.
In bacteria two forms of metabolic energy are usually present, i.e. ATP and transmembrane ion-gradients, that can be used to drive the various endergonic reactions associated with cellular growth....
Uniport of monoanionic L-malate in membrane vesicles from Leuconostoc oenos (1994)
Lolkema, Juke S., Poolman, Bert, Salema, Madalena, Loureiro Dias, Maria C., Konings, Wil N.
L-malate transport was studied in membrane vesicles from Leuconostoc oenos MLE(-) (mutant lacking malolactic enzyme) which were fused with liposomes containing beef heart cytochrome c oxidase as a...
Poolman, Bert, Veen, Hendrik W. Van, Poelarends, Gerrit, Molenaar, Douwe, Bolhuis, Henk, Driessen, Arnold J.M., ...
Three mutants of Lactococcus lactis subsp. lactis MG1363, termed EthR, DauR, and RhoR, were selected for resistance to high concentrations of ethidium bromide, daunomycin, and rhodamine 6G,...
Mechanism of Maltose Uptake and Glucose Excretion in Lactobacillus sanfrancisco (1994)
Konings, Wil N., Poolman, Bert, Hammes, Walter P., Glaasker, Erwin, Neubauer, Heike
Lactobacillus sanfrancisco LTH 2581 can use only glucose and maltose as sources of metabolic energy. In maltose-metabolizing cells of L. sanfrancisco, approximately half of the internally generated...
Lolkema, Juke S., Santos, Helena, Poolman, Bert, Ramos, Ana, Konings, Wil N.
The mechanism and energetics of citrate transport in Leuconostoc oenos were investigated. Resting cells of L. oenos generate both a membrane potential (ΔΨ) and a pH gradient (ΔpH) upon addition of...
The F- or V-Type Na+-ATPase of the Thermophilic Bacterium Clostridium fervidus (1994)
Konings, Wil N., Abee, Tjakko, Poolman, Bert, Speelmans, Gea
Clostridium fervidus is a thermophilic, anaerobic bacterium which uses solely Na+ as a coupling ion for energy transduction. Important features of the primary Na+ pump (ATPase) that generates the...
Poolman, Bert, Leenhouts, Kees J., Kunji, Edmund R.S., Hagting, Anja, Konings, Wil N.
Lactococcus lactis takes up di- and tripeptides via a proton motive force-dependent carrier protein. The gene (dtpT) encoding the di-tripeptide transport protein of L. lactis was cloned by...
Stability and proton-permeability of liposomes composed of archaeal tetraether lipids (1994)
Elferink, Marieke G.L., Wit, Janny G. De, Driessen, Arnold J.M., Konings, Wil N.
Liposomes composed of tetraether lipids originating from the thermoacidophilic archaeon Sulfolobus acidocaldarius were analyzed for their stability and proton permeability from 20°C up to 80°C. At...
Hillenga, Dirk J., Versantvoort, Hanneke J.M., Driessen, Arnold J.M., Konings, Wil N.
Functional plasma membranes from the filamentous fungus Penicillium chrysogenum have been isolated with the objective of studying transport processes. The isolation procedure consists of three steps,...
Gleissner, Michael, Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N., Anemüller, Stefan, Schäfer, Günter
The terminal quinol oxidase of the cytochrome aa3 type was isolated from the extreme thermo-acidophilic archaeon Sulfolobus acidocaldarius. In micellar solution, the enzyme oxidized various quinols...
Rowe, John J., Ubbink-Kok, Trees, Molenaar, Douwe, Konings, Wil N., Driessen, Arnold J.M.
Escherichia coli can use nitrate as a terminal electron acceptor for anaerobic respiration. A polytopic membrane protein, termed NarK, has been implicated in nitrate uptake and nitrite excretion and...
Mierau, Igor, Haandrikman, Alfred J., Velterop, Odilia, Tan, Paris S.T., Leenhouts, Kees L., Konings, WIL N., ...
The gene encoding a tripeptidase (pepT) of Lactococcus lactis subsp. cremoris (formerly subsp. lactis) MG1363 was cloned from a genomic library in pUC19 and subsequently sequenced. The tripeptidase...
Solute transport and energy transduction in bacteria (1994)
Poolman, Bert, Konings, Wil N., Veen, Hendrik W. Van
In bacteria two forms of metabolic energy are usually present, i.e. ATP and transmembrane ion-gradients, that can be used to drive the various endergonic reactions associated with cellular growth....
Uniport of monoanionic L-malate in membrane vesicles from Leuconostoc oenos (1994)
Poolman, Bert, Salema, Madalena, Lolkema, Juke S., Loureiro Dias, Maria C., Konings, Wil N.
L-malate transport was studied in membrane vesicles from Leuconostoc oenos MLE(-) (mutant lacking malolactic enzyme) which were fused with liposomes containing beef heart cytochrome c oxidase as a...
Veen, Hendrik W. Van, Poelarends, Gerrit, Molenaar, Douwe, Bolhuis, Henk, Poolman, Bert, Driessen, Arnold J.M., ...
Three mutants of Lactococcus lactis subsp. lactis MG1363, termed EthR, DauR, and RhoR, were selected for resistance to high concentrations of ethidium bromide, daunomycin, and rhodamine 6G,...
Mechanism of Maltose Uptake and Glucose Excretion in Lactobacillus sanfrancisco (1994)
Konings, Wil N., Poolman, Bert, Hammes, Walter P., Glaasker, Erwin, Neubauer, Heike
Lactobacillus sanfrancisco LTH 2581 can use only glucose and maltose as sources of metabolic energy. In maltose-metabolizing cells of L. sanfrancisco, approximately half of the internally generated...
Konings, Wil N., Lolkema, Juke S., Santos, Helena, Poolman, Bert, Ramos, Ana
The mechanism and energetics of citrate transport in Leuconostoc oenos were investigated. Resting cells of L. oenos generate both a membrane potential (ΔΨ) and a pH gradient (ΔpH) upon addition of...
The F- or V-Type Na+-ATPase of the Thermophilic Bacterium Clostridium fervidus (1994)
Konings, Wil N., Abee, Tjakko, Poolman, Bert, Speelmans, Gea
Clostridium fervidus is a thermophilic, anaerobic bacterium which uses solely Na+ as a coupling ion for energy transduction. Important features of the primary Na+ pump (ATPase) that generates the...
Leenhouts, Kees J., Kunji, Edmund R.S., Hagting, Anja, Poolman, Bert, Konings, Wil N.
Lactococcus lactis takes up di- and tripeptides via a proton motive force-dependent carrier protein. The gene (dtpT) encoding the di-tripeptide transport protein of L. lactis was cloned by...
Konings, Wil N., Poolman, Bert, Hillenga, Dirk, Speelmans, Gea
Reaction centers (RCs) were purified from the thermophilic phototrophic bacterium Chloroflexus aurantiacus and reconstituted into liposomes. The dependence of cyclic electron transfer via horse-heart...
Poolman, Bert, Plapp, Roland, Smid, Eddy J., Kunji, Edmund R.S., Konings, Wil N.
Lactococcus lactis ML3 possesses two different peptide transport systems of which the substrate size restriction and specificity have been determined. The first system is the earlier-described proton...
Uptake of 4-Toluene Sulfonate by Comamonas testosteroni T-2 (1993)
Konings, Wil N., Cook, Alasdair M., Poolman, Bert, Locher, Hans H.
The mechanism of transport of the xenobiotic 4-toluene sulfonate (TS) in Comamonas testosteroni T-2 was investigated. Rapid uptake of TS was observed only in cells grown with TS or 4-methylbenzoate...
Konings, Wil N., Poolman, Bert, Speelmans, Gea
Amino acid transport was studied in membranes of the peptidolytic, thermophilic, anaerobic bacterium Clostridium fervidus. Uptake of the negatively charged amino acid L-glutamate, the neutral amino...
Konings, Wil N., Abee, Tjakko, Poolman, Bert, Speelmans, Gea
The thermophilic, peptidolytic, anaerobic bacterium Closidium fervidus is unable to generate a pH gradient in the range of 5.5-8.0, which limits growth of the organism to a narrow pH range (6.3-7.7)....
Bacterial solute transport proteins in their lipid environment (1993)
Veld, Gerda In 't, Driessen, Arnold J.M., Konings, Wil N.
The cytoplasmic membrane of bacteria is a selective barrier that restricts entry and exit of solutes. Transport of solutes across this membrane is catalyzed by specific membrane proteins. Integral...
Elferink, Marieke G.L., Wit, Janny G. De, Driessen, Arnold J.M., Konings, Wil N.
Archaeal lipids differ considerably from eubacterial and eukaryotic lipids in their structure and physical properties. From the membranes of the extreme thermophilic archaea Sulfolobus acidocaldarius...
García Garcerá, Maria J., Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N.
Nisin is a lantibiotic produced by some strains of Lactococcus luctis subsp. lactis. The target for nisin action is the cytoplasmic membrane of Gram-positive bacteria. Nisin dissipates the membrane...
Iwaarden, Pierre R. Van, Driessen, Arnold J.M., Lolkema, Juke S., Kaback, H. Ronald, Konings, Wil N.
In this study, wild-type lac permease and lac permease mutated at each of the eight cysteinyl residues in the molecule were solubilized from the membrane, purified, and reconstituted into...
Chikindas, Michael L., García-Garcerá, Maria J., Driessen, Arnold J.M., Ledeboer, Aat M., Nissen-Meyer, Jon, Nes, Ingolf F., ...
Pediocin PA-1 is a bacteriocin which is produced by Pediococcus acidilactici PAC1.0. We demonstrate that pediocin PA-1 kills sensitive Pediococcus cells and acts on the cytoplasmic membrane. In...
Molenaar, Douwe, Bosscher, Jaap S., Brink, Bart Ten, Driessen, Arnold J.M., Konings, Wil N.
Lactobaciflus buchneri ST2A vigorously decarboxylates histidine to the biogenic amine histamine, which is excreted into the medium. Cells grown in the presence of histidine generate both a...
Molenaar, Douwe, Hagting, Anja, Alkema, Harmen, Driessen, Arnold J.M., Konings, Wil N.
Lactococcus lactis subsp. lacti ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A...
Bourdineaud, Jean-Paul, Heierli, Daniel, Gamper, Marianne, Verhoogt, Hans J.C., Driessen, Arnold J.M., Konings, Wil N., ...
The arcDABC operon of Pseudomonas aeruginosa encodes the enzymes of the arginine deiminase pathway and is induced by oxygen limitation. The arcD gene specifies a 53-kDa protein with...
Mierau, Igor, Tan, Paris S.T., Haandrikman, Alfred J., Kok, Jan, Leenhouts, Kees J., Konings, Wil N., ...
The gene specifying an endopeptidase of Lactococcus lactis, named pepO, was cloned from a genomic library of L. lactis subsp. cremoris P8-2-47 in λEMBL3 and was subsequently sequenced. pepO is...
Poolman, Bert, Hillenga, Dirk, Speelmans, Gea, Konings, Wil N.
Reaction centers (RCs) were purified from the thermophilic phototrophic bacterium Chloroflexus aurantiacus and reconstituted into liposomes. The dependence of cyclic electron transfer via horse-heart...
Kunji, Edmund R.S., Smid, Eddy J., Plapp, Roland, Poolman, Bert, Konings, Wil N.
Lactococcus lactis ML3 possesses two different peptide transport systems of which the substrate size restriction and specificity have been determined. The first system is the earlier-described proton...
Uptake of 4-Toluene Sulfonate by Comamonas testosteroni T-2 (1993)
Konings, Wil N., Cook, Alasdair M., Poolman, Bert, Locher, Hans H.
The mechanism of transport of the xenobiotic 4-toluene sulfonate (TS) in Comamonas testosteroni T-2 was investigated. Rapid uptake of TS was observed only in cells grown with TS or 4-methylbenzoate...
Konings, Wil N., Poolman, Bert, Speelmans, Gea
Amino acid transport was studied in membranes of the peptidolytic, thermophilic, anaerobic bacterium Clostridium fervidus. Uptake of the negatively charged amino acid L-glutamate, the neutral amino...
Konings, Wil N., Abee, Tjakko, Poolman, Bert, Speelmans, Gea
The thermophilic, peptidolytic, anaerobic bacterium Closidium fervidus is unable to generate a pH gradient in the range of 5.5-8.0, which limits growth of the organism to a narrow pH range (6.3-7.7)....
Uptake of 4-toluene sulfonate by Comamonas testosteroni T-2 (1993)
Locher, Hans H., Poolman, Bert, Cook, Alasdair M., Konings, Wil N.
The mechanism of transport of the xenobiotic 4-toluene sulfonate (TS) in Comamonas testosteroni T-2 was investigated. Rapid uptake of TS was observed only in cells grown with TS or 4-methylbenzoate...
Can the excretion of metabolites by bacteria be manipulated? (1992)
Driessen, Arnold J.M., Poolman, Bert, Konings, Wil N.
Bacteria can release metabolites into the environment by various mechanisms. Excretion may occur by passive diffusion or by the reversal of the uptake process when the internal concentration of the...
Konings, Wil N., Poolman, Bert, Abee, Tjakko, Bolhuis, Henk, Molenaar, Douwe
Many bacteria, both gram positive and gram negative, extrude in an energy-dependent manner the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5[and -6]-carboxyfluorescein (BCECF). This efflux...
Konings, Wil N., Poolman, Bert, Sikkema, Jan
Tetralin is toxic to bacterial cells at concentrations below 100 μmol/liter. To assess the inhibitory action of tetralin on bacterial membranes, a membrane model system, consisting of...
Konings, Wil N., Poolman, Bert, Tolner, Berend
The genes encoding the Na+/H+/L-glutamate symport proteins of the thermophilic organisms Bacillus stearothermophilus (gltTBs) and Bacillus caldotenax (gltTBc) were cloned by complementation of...
What we can learn from the effects of thiol reagents on transport proteins (1992)
Iwaarden, Pierre R. Van, Driessen, Arnold J.M., Konings, Wil N.
Many secondary membrane transport systems contain reactive sulfhydryl groups. In this review the applications of SH reagents for analyzing the role of sulfhydryl groups in membrane transport systems...
Veld, Gerda In 't, Vrije, Truus De, Driessen, Arnold J.M., Konings, Wil N.
The branched-chain amino acid transport system of Lactococcus lactis was solubilized with n-octyl β-D-glucopyranoside and reconstituted into proteoliposomes. Transport activity was recovered only...
Veld, Gerda In 't, Driessen, Arnold J.M., Konings, Wil N.
The effect of the degree of unsaturation of the phospholipid acyl chains on the branched-chain amino acid transport system of Lactococcus lactis was investigated by the use of a membrane fusion...
Elferink, Marieke G.L., Wit, Janny G. De, Veld, Gerda In 't, Reichert, Anke, Driessen, Arnold J.M., Ringsdorf, Helmut, ...
Liposomes composed of Escherichia coli phospholipid were coated with polysaccharides bearing hydrophohic palmitoyl anchors. The effect on the stability of liposomes without or with integral membrane...
Verhoogt, Hans J.C., Smit, Hans, Abee, Tjakko, Gamper, Marianne, Driessen, Arnold J.M., Haas, Dieter, ...
In the absence of oxygen and nitrate, Pseudomonas aeruginosa metabolizes arginine via the arginine deiminase pathway, which allows slow growth on rich media. The conversion of arginine to ornithine,...
Elferink, Marieke G.L., Wit, Janny G. De, Demel, Rudy, Driessen, Arnold J.M., Konings, Wil N.
Membranes of Sulfolobus acidocaldarius, an extreme thermophilic archaebacterium, are composed of unusual bipolar lipids. They consist of macrocyclic tetraethers with two polar heads linked by two...
Veld, Gerda In ’t, Elferink, Marieke G.L., Driessen, Arnold J.M., Konings, Wil N.
The effect of bipolar tetraether lipids, extracted from the thermophilic archaebacterium Sulfolobus acidocaldarius, on the branched-chain amino acid transport system of the mesophilic bacterium...
Energy Transduction and Transport Processes in Thermophilic Bacteria (1992)
Konings, Wil N., Tolner, Berend, Speelmans, Gea, Elferink, Marieke G.L., Wit, Janny G. De, Driessen, Arnold J.M.
Bacterial growth at the extremes of temperature has remained a fascinating aspect in the study of membrane function and structure. The stability of the integral membrane proteins of thermophiles make...
Laan, Harry, Kok, Jan, Haandrikman, Alfred J., Venema, Gerard, Konings, Wil N.
Lactococcus lactis strains produce an extracellular subtilisin-related serine proteinase in which immunologically different components can be distinguished. Monoclonal antibodies specific for the...
Can the excretion of metabolites by bacteria be manipulated? (1992)
Driessen, Arnold J.M., Poolman, Bert, Konings, Wil N.
Bacteria can release metabolites into the environment by various mechanisms. Excretion may occur by passive diffusion or by the reversal of the uptake process when the internal concentration of the...
Konings, Wil N., Poolman, Bert, Abee, Tjakko, Bolhuis, Henk, Molenaar, Douwe
Many bacteria, both gram positive and gram negative, extrude in an energy-dependent manner the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5[and -6]-carboxyfluorescein (BCECF). This efflux...
Konings, Wil N., Poolman, Bert, Sikkema, Jan
Tetralin is toxic to bacterial cells at concentrations below 100 μmol/liter. To assess the inhibitory action of tetralin on bacterial membranes, a membrane model system, consisting of...
Konings, Wil N., Poolman, Bert, Tolner, Berend
The genes encoding the Na+/H+/L-glutamate symport proteins of the thermophilic organisms Bacillus stearothermophilus (gltTBs) and Bacillus caldotenax (gltTBc) were cloned by complementation of...
Hemme, Denis, Poolman, Bert, Winters, David A., Konings, Wil N.
Membrane vesicles of Leuconostoc mesenteroides subsp. dextranicum fused with proteoliposomes prepared from Escherichia coli phospholipids containing beef heart cytochrome c oxidase were used to study...
Renault, Pierre P., Abee, Tjakko, Ubbink, Trees, Smid, Eddy J., Molenaar, Douwe, Poolman, Bert, ...
The mechanism of metabolic energy production by malolactic fermentation in Lactococcus lactis has been investigated. In the presence of L-malate, a proton motive force composed of a membrane...
Driessen, Arnold J.M., Veld, Gerda In 't, Konings, Wil N.
The effect of the phospholipid acyl chain carbon number on the activity of the branched-chain amino acid transport system of Lactococcus lactis has been investigated. Major fatty acids identified in...
Processing of the Lactococcal Extracellular Serine Proteinase (1991)
Haandrikman, Alfred J., Meesters, Rene, Laan, Harry, Konings, Wil N., Kok, Jan, Venema, Gerard
Activity of the lactococcal cell envelope-located serine proteinase depends on the presence of membrane-associated lipoprotein PrtM. To differentiate between the action of the proteinase and the...
Belkum, Marco J. Van, Kok, Jan, Venema, Gerard, Holo, Helge, Nes, Ingolf F., Konings, Wil N., ...
Lactococcin A is a bacteriocin produced by Lactococcus lactis. Its structural gene has recently been cloned and sequenced. Purified lactococcin A increased the permeability of the cytoplasmic...
Poolman, Bert, Winters, David A., Hemme, Denis, Konings, Wil N.
Membrane vesicles of Leuconostoc mesenteroides subsp. dextranicum fused with proteoliposomes prepared from Escherichia coli phospholipids containing beef heart cytochrome c oxidase were used to study...
Ubbink, Trees, Smid, Eddy J., Molenaar, Douwe, Poolman, Bert, Abee, Tjakko, Renault, Pierre P., ...
The mechanism of metabolic energy production by malolactic fermentation in Lactococcus lactis has been investigated. In the presence of L-malate, a proton motive force composed of a membrane...
Driessen, Arnold J.M., Konings, Wil N.
The effect of various sulfhydryl (SH)-specific reagents on arginine-ornithine antiport activity in membrane vesicles of Lactococcus lactis was studied. Little or no inhibition of arginine-ornithine...
Energy-Dependent Uptake of 4-Chlorobenzoate in the Coryneform Bacterium NTB-1 (1990)
Groenewegen, Peter E.J., Driessen, Arnold J.M., Konings, Wil N.
The uptake of 4-chlorobenzoate (4-CBA) in intact cells of the coryneform bacterium NTB-1 was investigated. Uptake and metabolism of 4-CBA were observed in cells grown in 4-CBA but not in...
Kinetic Mechanism and Specificity of the Arginine-Ornithine Antiporter of Lactococcus lactis (1989)
Driessen, Arnold J.M., Molenaar, Douwe, Konings, Wil N.
The kinetic mechanism and specificity of the arginine-ornithine antiporter was investigated in membrane vesicles derived from Lactococcus lactis. Membrane vesicles loaded with ornithine, and diluted...
Transport of Basic Amino Acids by Membrane Vesicles of Lactococcus lactis (1989)
Driessen, Arnold J.M., Leeuwen, Cornelis Van, Konings, Wil N.
The uptake of the basic amino acids arginine, ornithine, and lysine was studied in membrane vesicles derived from cells of Lactococcus lactis which were fused with liposomes in which beef heart...
Strobel, Herbert J., Russell, James B., Driessen, Arnold J.M., Konings, Wil N.
Lactobacilus casei 393 cells which were energized with glucose (pH 6.0) took up glutamine, asparagine, glutamate, aspartate, leucine, and phenylalanine. Little or no uptake of several essential amino...
Mechanism and Energetics of Dipeptide Transport in Membrane Vesicles of Lactococcus lactis (1989)
Smid, Eddy J., Driessen, Arnold J.M., Konings, Wil N.
Alanyl-α-glutamate transport has been studied in Lactococcus lactis ML3 cells and in membrane vesicles fused with liposomes containing beefheart cytochrome c oxidase as a...
Haandrikman, Alfred J., Kok, Jan, Laan, Harry, Soemitro, Soetijoso, Ledeboer, Aat M., Konings, Wil N., ...
Directly upstream of the Lactococcus lactis subsp. cremoris Wg2 proteinase gene is an oppositely directed open reading frame (ORF1). The complete nucleotide sequence of ORF1, encoding a 33-kilodalton...
Relation of Growth of Streptococcus lactis and Streptococcus cremoris to Amino Acid Transport (1988)
Konings, Wil N., Poolman, Bert
The maximum specific growth rate of Streptococcus lactis and Streptococcus cremoris on synthetic medium containing glutamate but no glutamine decreases rapidly above pH 7. Growth of these organisms...
Driessen, Arnold J.M., Ubbink-Kok, Trees, Konings, Wil N.
Membrane vesicles were isolated from the obligate anaerobic bacterium Clostridium acetobutylicum. Beef heart mitochondrial cytochrome c oxidase was inserted in these membrane vesicles by membrane...
Driessen, Arnold J.M., Smid, Eddy J., Konings, Wil N.
Enterococcusfaecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2,...
Russell, James B., Strobel, Herbert J., Driessen, Arnold J.M., Konings, Wil N.
Streptococcus bovis JB1 cells were able to transport serine, threonine, or alanine, but only when they were incubated in sodium buffers. If glucose-energized cells were washed in potassium phosphate...
Zheng, Tan, Driessen, Arnold J.M., Konings, Wil N.
The effect of cholesterol on the activity of the branched-chain amino acid transport system of Streptococcus cremoris was studied in membrane vesicles of S. cremoris fused with liposomes made of egg...
Crielaard, Wim, Driessen, Arnold J.M., Molenaar, Douwe, Hellingwerf, Klaas J., Konings, Wil N.
Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were introduced as proton motive force-generating systems in membrane vesicles of two anaerobic bacteria. Liposomes...
Lipid Requirement of the Branched-Chain Amino Acid Transport System of Streptococcus cremoris (1988)
Driessen, Arnold J.M., Zheng, Tan, Veld, Gerda In ’t, Konings, Wil N.
The role of the membrane lipid composition on the transport protein of branched-chain amino acids of the homofermentative lactic acid bacterium Streptococcus cremoris has been investigated. The major...
Relation of Growth of Streptococcus lactis and Streptococcus cremoris to Amino Acid Transport (1988)
Konings, Wil N., Poolman, Bert
The maximum specific growth rate of Streptococcus lactis and Streptococcus cremoris on synthetic medium containing glutamate but no glutamine decreases rapidly above pH 7. Growth of these organisms...
Konings, Wil N., Veldkamp, Hans, Smid, Eddy J., Poolman, Bert
Streptococcus cremoris cells that had been grown in a chemostat were starved for lactose. The viability of the culture remained essentially constant in the first hours of starvation and subsequently...
Konings, Wil N., Hellingwerf, Klaas J., Poolman, Bert
Various methods of manipulation of the intracellular pH in Streptococcus lactis result in a unique relationship between the rate of glutamate and glutamine transport and the cytoplasmic pH. The...
Konings, Wil N., Smid, Eddy J., Poolman, Bert
In Streptococcus lactis ML3 and Streptococcus cremoris Wg2 the uptake of glutamate and glutamine is mediated by the same transport system, which has a 30-fold higher affinity for glutamine than for...
Konings, Wil N., Kiers, Jan, Bosman, Boukje, Poolman, Bert
The decreased response of the energy metabolism of lactose-starved Streptococcus cremoris upon readdition of lactose is caused by a decrease of the glycolytic activity. The decrease in glycolysis is...
Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger (1987)
Kiewiet, Rense, Poolman, Bert, Driessen, Arnold J.M., Konings, Wil N.
Streptococcus lactis metabolizes arginine via the arginine deiminase pathway to ornithine, CO2, NH3, and ATP. The translocation of arginine and ornithine has been studied using membrane vesicles of...
Driessen, Arnold J.M., Hellingwerf, Klaas J., Konings, Wil N.
Bacteriorhodopsin and trypsin-modified bacteriorhodopsin have been reconstituted into liposomes by means of a low pH-sonication procedure. The incorporation of bacteriorhodopsin in these...
Protonmotive-force-driven leucine uptake in yeast plasma membrane vesicles (1987)
Opekarovà, Miroslava, Driessen, Arnold J.M., Konings, Wil N.
Plasma membranes prepared from Saccharomyces cerevisiae by the concanavalin A method were fused with liposomes by a freeze-thaw sonication method. A Δψ of -109 mV was generated in the sealed...
Driessen, Arnold J.M., Kodde, Jan, Jong, Steven De, Konings, Wil N.
The pH dependence of transport of the neutral amino acids L-serine and L-alanine by membrane vesicles of Streptococcus cremoris have been studied in detail. The rates of four modes of facilitated...
Transport of Branched-Chain Amino Acids in Membrane Vesicles of Streptococcus cremoris (1987)
Driessen, Arnold J.M., Jong, Steven De, Konings, Wil N.
The kinetics, specificity, and mechanism of branched-chain amino acid transport in Streptococcus cremoris were studied in a membrane system of S. cremoris in which beef heart mitochondrial cytochrome...
Driessen, Arnold J.M., Hellingwerf, Klaas J., Konings, Wil N.
The energetics of neutral and branched chain amino acid transport by membrane vesicles from Streptococcus cremoris have been studied with a novel model system in which beef heart mitochondrial...
Cell Wall-Associated Proteases of Streptococcus cremoris Wg2 (1987)
Hugenholtz, Jeroen, Sinderen, Douwe Van, Kok, Jan, Konings, Wil N.
Two components of the proteolytic system, proteins A and B, have been studied in Streptococcus cremoris Wg2 by immunological methods. The components could not be separated by standard chromatography...
Konings, Wil N., Veldkamp, Hans, Smid, Eddy J., Poolman, Bert
Streptococcus cremoris cells that had been grown in a chemostat were starved for lactose. The viability of the culture remained essentially constant in the first hours of starvation and subsequently...
Konings, Wil N., Hellingwerf, Klaas J., Poolman, Bert
Various methods of manipulation of the intracellular pH in Streptococcus lactis result in a unique relationship between the rate of glutamate and glutamine transport and the cytoplasmic pH. The...
Konings, Wil N., Smid, Eddy J., Poolman, Bert
In Streptococcus lactis ML3 and Streptococcus cremoris Wg2 the uptake of glutamate and glutamine is mediated by the same transport system, which has a 30-fold higher affinity for glutamine than for...
Kiers, Jan, Bosman, Boukje, Poolman, Bert, Konings, Wil N.
The decreased response of the energy metabolism of lactose-starved Streptococcus cremoris upon readdition of lactose is caused by a decrease of the glycolytic activity. The decrease in glycolysis is...
Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger (1987)
Poolman, Bert, Driessen, Arnold J.M., Kiewiet, Rense, Konings, Wil N.
Streptococcus lactis metabolizes arginine via the arginine deiminase pathway to ornithine, CO2, NH3, and ATP. The translocation of arginine and ornithine has been studied using membrane vesicles of...
Vrij, Wim De, Driessen, Arnold J.M., Hellingwerf, Klaas J., Konings, Wil N.
Cytochrome c oxidase from Bacillus subtilis was reconstituted in liposomes and its energy-transducing properties were studied, The reconstitution procedure used included Ca2+-induced fusion of...
Driessen, Arnold J.M., Vrij, Wim De, Konings, Wil N.
Beef heart mitochondrial cytochrome c oxidase has been incorporated into membrane vesicles derived from the homofermentative lactic acid bacterium Streptococcus cremoris. Proteoliposomes containing...
Calcium transport in membrane vesicles of Streptococcus cremoris (1986)
Driessen, Arnold J.M., Konings, Wil N.
Rightside-out membrane vesicles of Streptococcus cremoris were fused with proteoliposomes containing the light-driven proton pump bacteriorhodopsin by a low-pH fusion procedure reported earlier. In...
Driessen, Arnold J.M., Hellingwerf, Klaas J., Konings, Wil N.
The light-driven primary proton pump bacteriorhodopsin has been incorporated in the cytoplasmic membrane of Streptococcus cremoris, in order to generate a protonmotive force across these membranes....
Driessen, Arnold J.M., Vrij, Wim De, Konings, Wil N.
Membrane vesicles derived from the strictly fermentative lactic acid bacterium Streptococcus cremoris have been fused with proteoliposomes containing the beef heart mitochondrial cytochrome c oxidase...
Poolman, Bert, Belkum, Marco J. Van, Hellingwerf, Klaas J., Elferink, Marieke G.L., Konings, Wil N.
In studies on alanine and lactose transport in Rhodopseudomonas sphaeroides we have demonstrated that the rate of solute uptake in this phototrophic bacterium is regulated by the rate of...
Poolman, Bert, Belkum, Marco J. Van, Hellingwerf, Klaas J., Elferink, Marieke G.L., Konings, Wil N.
In studies on alanine and lactose transport in Rhodopseudomonas sphaeroides we have demonstrated that the rate of solute uptake in this phototrophic bacterium is regulated by the rate of...
Robillard, George T., Konings, Wil N., Poolman, Bert
Evidence is presented in this report for the presence of two sets of dithiols associated with proline transport activity in Escherichia coli. One set is located at the outer surface, the other at the...
Robillard, George T., Konings, Wil N., Poolman, Bert
Evidence is presented in this report for the presence of two sets of dithiols associated with proline transport activity in Escherichia coli. One set is located at the outer surface, the other at the...
Lolkema, Juke S., Abbing, Arend, Hellingwerf, Klaas J., Konings, Wil N.
The membrane potential was determined in intact cells of Rhodopseudomonas sphaeroides from the distribution of the lipophilic cation tetraphenylphosphonium (Ph4P+) after correction for probe binding...
Robillard, George T., Konings, Wil N., Poolman, Bert
Evidence is presented in this report for the presence of two sets of dithiols associated with proline transport activity in Escherichia coli. One set is located at the outer surface, the other at the...
Konings, Wil N., Robillard, George T.
We have recently shown that the physical mechanism for Δµ~H+-driven changes in the Km for three different transport systems is an oxidation-reduction reaction involving a dithiol-disulfide...
Lolkema, Juke S., Hellingwerf, Klaas J., Konings, Wil N.
The electrical potential across the cytoplasmic membrane of bacteria can be calculated from the distribution of the lipophilic cation tetraphenylphosphonium between the bulk phases of the medium and...
Energy Coupling of Facilitated Transport of Inorganic Ions in Rhodopseudomonas sphaeroides (1982)
Hellingwerf, Klaas J., Friedberg, Ilan, Lolkema, Juke S., Michels, Paul A.M., Konings, Wil N.
Within the scope of a study on the effects of changes in medium composition on the proton motive force in Rhodopseudomonas sphaeroides, the energy coupling of sodium, phosphate, and potassium...
Dijkhuizen, Lubbert, Boer, Lex De, Boers, Roelof H., Harder, Wim, Konings, Wil N.
Cytoplasmic membrane vesicles were prepared by a lysozyme-salt treatment from Arthrobacter P1 grown on methylamine as the carbon and energy source. In the presence of an ascorbate-phenazine...
Konings, Wil N., Robillard, George T.
We have recently shown that the physical mechanism for Δµ~H+-driven changes in the Km for three different transport systems is an oxidation-reduction reaction involving a dithiol-disulfide...
Variable stoichiometry of proton:lactose symport in Escherichia coli cells (1981)
Brink, Bart Ten, Lolkema, Juke S., Hellingwerf, Klaas J., Konings, Wil N.
Michels, Paul A.M., Hellingwerf, Klaas J., Lolkema, Juke S., Friedberg, Ilan, Konings, Wil N.
The magnitude and composition of the proton motive force (Δµ~H+) has been measured in chromatophores and whole cells of Rhodopseudomonas sphaeroides as a function of the ionic composition of the...
Detmers, Frank J. M., Lanfermeijer, Frank C., Abele, Rupert, Jack, Ralph W., Tampé, Robert, Konings, Wil N., ...
The oligopeptide transport system (Opp) of Lactococcus lactis has the unique capacity to mediate the transport of peptides from 4 up to at least 18 residues. The substrate specificity of this binding...
Slotboom, Dirk Jan, Sobczak, Iwona, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft. The proteins belong to a large family of secondary transporters, which includes...
Sulfur Regulation of the Sulfate Transporter Genes sutA and sutB in Penicillium chrysogenum
Van De Kamp, Mart, Schuurs, Theo A., Vos, Arnold, Van Der Lende, Ted R., Konings, Wil N., Driessen, Arnold J. M.
Penicillium chrysogenum uses sulfate as a source of sulfur for the biosynthesis of penicillin. Sulfate uptake and the mRNA levels of the sulfate transporter-encoding sutB and sutA genes are all...
Mechanism of Citrate Metabolism in Lactococcus lactis: Resistance against Lactate Toxicity at Low pH
Magni, Christian, De Mendoza, Diego, Konings, Wil N., Lolkema, Juke S.
Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was...
Albers, Sonja-V., Elferink, Marieke G. L., Charlebois, Robert L., Sensen, Christoph W., Driessen, Arnold J. M., Konings, Wil N.
The archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This...
Complementary and Overlapping Selectivity of the Two-Peptide Bacteriocins Plantaricin EF and JK
Moll, Gert N., Van Den Akker, Emile, Hauge, Håvard H., Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., ...
Plantaricin EF and JK are both two-peptide bacteriocins produced by Lactobacillus plantarum C11. The mechanism of plantaricin EF and JK action was studied on L. plantarum 965 cells. Both plantaricins...
Picon, Antonia, Kunji, Edmund R. S., Lanfermeijer, Frank C., Konings, Wil N., Poolman, Bert
The kinetic properties of wild-type and mutant oligopeptide binding proteins of Lactococcus lactis were determined. To observe the properties of the mutant proteins in vivo, the oppA gene was deleted...
Yokota, Atsushi, Veenstra, Marloes, Kurdi, Peter, Van Veen, Hendrik W., Konings, Wil N.
The cholate-resistant Lactococcus lactis strain C41-2, derived from wild-type L. lactis MG1363 through selection for growth on cholate-containing medium, displayed a reduced accumulation of cholate...
Catabolite Repression and Induction of the Mg2+-Citrate Transporter CitM of Bacillus subtilis
Warner, Jessica B., Krom, Bastiaan P., Magni, Christian, Konings, Wil N., Lolkema, Juke S.
In Bacillus subtilis the citM gene encodes the Mg2+-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region,...
Krom, Bastiaan P., Warner, Jessica B., Konings, Wil N., Lolkema, Juke S.
Citrate uptake in Bacillus subtilis is stimulated by a wide range of divalent metal ions. The metal ions were separated into two groups based on the expression pattern of the uptake system. The two...
Cholic Acid Is Accumulated Spontaneously, Driven by Membrane ΔpH, in Many Lactobacilli
Kurdi, Peter, Van Veen, Hendrik W., Tanaka, Hiroshi, Mierau, Igor, Konings, Wil N., Tannock, Gerald W., ...
Many lactobacilli from various origins were found to apparently lack cholic acid extrusion activity. Cholic acid was accumulated spontaneously, driven by the transmembrane proton gradient....
Koning, Sonja M., Elferink, Marieke G. L., Konings, Wil N., Driessen, Arnold J. M.
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different β-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an...
Sakamoto, Kanta, Margolles, Abelardo, Van Veen, Hendrik W., Konings, Wil N.
Lactobacillus brevis is a major contaminant of spoiled beer. The organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. The hop...
Structural Features of the Glutamate Transporter Family
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft and thus prevent neurotoxicity. The proteins belong to a large and widespread family...
Molecular Properties of Bacterial Multidrug Transporters
Putman, Monique, Van Veen, Hendrik W., Konings, Wil N.
One of the mechanisms that bacteria utilize to evade the toxic effects of antibiotics is the active extrusion of structurally unrelated drugs from the cell. Both intrinsic and acquired multidrug...
Van De Kamp, Mart, Pizzinini, Enrica, Vos, Arnold, Van Der Lende, Ted R., Schuurs, Theo A., Newbert, Roger W., ...
In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products...
Mechanistic Properties of the Two-Component Bacteriocin Lactococcin G
Moll, Gert, Hildeng-Hauge, Håvard, Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., Driessen, Arnold J. M.
Lactococcin G is a bacteriocin whose activity depends on the complementary action of two peptides, termed α and β. Biologically active, synthetic lactococcin G was used to study the mode of action...
Physiological Response of Lactobacillus plantarum to Salt and Nonelectrolyte Stress
Glaasker, Erwin, Tjan, Frans S. B., Ter Steeg, Pieter F., Konings, Wil N., Poolman, Bert
In this report, we compared the effects on the growth of Lactobacillus plantarum of raising the medium molarity by high concentrations of KCl or NaCl and iso-osmotic concentrations of nonionic...
Glaasker, Erwin, Konings, Wil N., Poolman, Bert
The accumulation of quaternary ammonium compounds in Lactobacillus plantarum is mediated via a single transport system with a high affinity for glycine betaine (apparent Km of 18 μM) and carnitine...
The Lantibiotic Nisin Induces Transmembrane Movement of a Fluorescent Phospholipid
Moll, Gert N., Konings, Wil N., Driessen, Arnold J. M.
Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid...
Fang, Gang, Konings, Wil N., Poolman, Bert
The peptide transport protein DtpT of Lactococcus lactis was purified and reconstituted into detergent-destabilized liposomes. The kinetics and substrate specificity of the transporter in the...
Membrane-Bound ATPase Contributes to Hop Resistance of Lactobacillus brevis
Sakamoto, Kanta, Van Veen, H. W., Saito, Hiromi, Kobayashi, Hiroshi, Konings, Wil N.
The activity of the membrane-bound H+-ATPase of the beer spoilage bacterium Lactobacillus brevis ABBC45 increased upon adaptation to bacteriostatic hop compounds. The ATPase activity was optimal...
Autoproteolysis of the Extracellular Serine Proteinase of Lactococcus lactis subsp. cremoris Wg2
The molecular masses of purified extracellular serine proteinase of a number of Lactococcus lactis strains vary significantly, and these molecular mass values do not correspond to the values...
Winters, David A., Poolman, Bert, Hemme, Denis, Konings, Wil N.
Membrane vesicles of Leuconostoc mesenteroides subsp. dextranicum fused with proteoliposomes prepared from Escherichia coli phospholipids containing beef heart cytochrome c oxidase were used to study...
Purification and Characterization of a Tripeptidase from Lactococcus lactis subsp. cremoris Wg2
Bosman, Boukje W., Tan, Paris S. T., Konings, Wil N.
A tripeptidase from a cell extract of Lactococcus lactis subsp. cremoris Wg2 has been purified to homogeneity by DEAE-Sephacel and phenyl-Sepharose chromatography followed by gel filtration over a...
Localization of Peptidases in Lactococci
Tan, Paris S. T., Chapot-Chartier, Marie-Pierre, Pos, Klaas M., Rousseau, Micheline, Boquien, Clair-Yves, Gripon, Jean-Claude, ...
The localization of two aminopeptidases, an X-prolyl-dipeptidyl aminopeptidase, an endopeptidase, and a tripeptidase in Lactococcus lactis was studied. Polyclonal antibodies raised against each...
Laan, Harry, Smid, Eddy J., De Leij, Loe, Schwander, Edwin, Konings, Wil N.
Twelve monoclonal antibodies directed to the cell-wall-associated proteinase of Lactococcus lactis subsp. cremoris Wg2 were isolated after immunization of BALB/c mice with a partially purified...
Mechanism of Proteinase Release from Lactococcus lactis subsp. cremoris Wg2
The procedure generally used for the isolation of extracellular, cell-associated proteinases of Lactococcus lactis species is based on the release of the proteinases by repeated incubation and...
Hugenholtz, Jeroen, Veldkamp, Hans, Konings, Wil N.
Antisera against four different strains of Streptococcus cremoris were raised by injecting rabbits with washed suspensions of whole cells. These antisera interacted specifically with the...
Selection of Protease-Positive and Protease-Negative Variants of Streptococcus cremoris
Hugenholtz, Jeroen, Splint, Rob, Konings, Wil N., Veldkamp, Hans
Protease-negative variants were shown to outcompete the wild-type strains of Streptococcus cremoris E8, HP, and Wg2 at pH values higher than 6.0 in milk. For S. cremoris E8 this process was studied...
Cell Wall-Associated Proteases of Streptococcus cremoris Wg2
Hugenholtz, Jeroen, Van Sinderen, Douwe, Kok, Jan, Konings, Wil N.
Two components of the proteolytic system, proteins A and B (J. Hugenholtz, F. Exterkate, and W. N. Konings, Appl. Environ. Microbiol. 48:1105-1110, 1984), have been studied in Streptococcus cremoris...
Mierau, Igor, Tan, Paris S. T., Haandrikman, Alfred J., Mayo, Balthasar, Kok, Jan, Leenhouts, Kees J., ...
Van Veen, Hendrik W., Margolles, Abelardo, Müller, Michael, Higgins, Christopher F., Konings, Wil N.
The bacterial LmrA protein and the mammalian multidrug resistance P-glycoprotein are closely related ATP-binding cassette (ABC) transporters that confer multidrug resistance on cells by mediating the...
Energy Supply for Active Transport in Anaerobically Grown Escherichia coli
Boonstra, Johannes, Downie, J. Allan, Konings, Wil N.
Escherichia coli K-12, grown under anaerobic conditions with glucose as the sole source of carbon and energy without any terminal electron acceptor added, contains a fumarate reductase system in...
Energetics of Leucyl-Leucine Hydrolysis in Streptococcus cremoris Wg2
Van Boven, Aart, Konings, Wil N.
The hydrolysis of the dipeptide leucyl-leucine by whole cells of Streptococcus cremoris Wg2 was dependent on the presence of the energy source lactose. Incubation of cells with uncouplers or ATPase...
The Proteolytic Systems of Streptococcus cremoris: an Immunological Analysis
Hugenholtz, Jeroen, Exterkate, Fred, Konings, Wil N.
The cell-wall-associated proteolytic systems of several Streptococcus cremoris strains were analyzed by crossed immunoelectrophoresis. At least four immunologically different components of the...
Lanfermeijer, Frank C., Detmers, Frank J.M., Konings, Wil N., Poolman, Bert
Lactococcus lactis degrades exogenous proteins such as β-casein to peptides of 4–30 amino acids, and uses these as nitrogen sources. The binding protein or receptor (OppALl) of the oligopeptide...
Detmers, Frank J. M., Lanfermeijer, Frank C., Abele, Rupert, Jack, Ralph W., Tampé, Robert, Konings, Wil N., ...
The oligopeptide transport system (Opp) of Lactococcus lactis has the unique capacity to mediate the transport of peptides from 4 up to at least 18 residues. The substrate specificity of this binding...
Slotboom, Dirk Jan, Sobczak, Iwona, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft. The proteins belong to a large family of secondary transporters, which includes...
Sulfur Regulation of the Sulfate Transporter Genes sutA and sutB in Penicillium chrysogenum
Van De Kamp, Mart, Schuurs, Theo A., Vos, Arnold, Van Der Lende, Ted R., Konings, Wil N., Driessen, Arnold J. M.
Penicillium chrysogenum uses sulfate as a source of sulfur for the biosynthesis of penicillin. Sulfate uptake and the mRNA levels of the sulfate transporter-encoding sutB and sutA genes are all...
Mechanism of Citrate Metabolism in Lactococcus lactis: Resistance against Lactate Toxicity at Low pH
Magni, Christian, De Mendoza, Diego, Konings, Wil N., Lolkema, Juke S.
Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was...
Albers, Sonja-V., Elferink, Marieke G. L., Charlebois, Robert L., Sensen, Christoph W., Driessen, Arnold J. M., Konings, Wil N.
The archaeon Sulfolobus solfataricus grows optimally at 80°C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This...
Complementary and Overlapping Selectivity of the Two-Peptide Bacteriocins Plantaricin EF and JK
Moll, Gert N., Van Den Akker, Emile, Hauge, Håvard H., Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., ...
Plantaricin EF and JK are both two-peptide bacteriocins produced by Lactobacillus plantarum C11. The mechanism of plantaricin EF and JK action was studied on L. plantarum 965 cells. Both plantaricins...
Picon, Antonia, Kunji, Edmund R. S., Lanfermeijer, Frank C., Konings, Wil N., Poolman, Bert
The kinetic properties of wild-type and mutant oligopeptide binding proteins of Lactococcus lactis were determined. To observe the properties of the mutant proteins in vivo, the oppA gene was deleted...
Yokota, Atsushi, Veenstra, Marloes, Kurdi, Peter, Van Veen, Hendrik W., Konings, Wil N.
The cholate-resistant Lactococcus lactis strain C41-2, derived from wild-type L. lactis MG1363 through selection for growth on cholate-containing medium, displayed a reduced accumulation of cholate...
Catabolite Repression and Induction of the Mg2+-Citrate Transporter CitM of Bacillus subtilis
Warner, Jessica B., Krom, Bastiaan P., Magni, Christian, Konings, Wil N., Lolkema, Juke S.
In Bacillus subtilis the citM gene encodes the Mg2+-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region,...
Krom, Bastiaan P., Warner, Jessica B., Konings, Wil N., Lolkema, Juke S.
Citrate uptake in Bacillus subtilis is stimulated by a wide range of divalent metal ions. The metal ions were separated into two groups based on the expression pattern of the uptake system. The two...
Cholic Acid Is Accumulated Spontaneously, Driven by Membrane ΔpH, in Many Lactobacilli
Kurdi, Peter, Van Veen, Hendrik W., Tanaka, Hiroshi, Mierau, Igor, Konings, Wil N., Tannock, Gerald W., ...
Many lactobacilli from various origins were found to apparently lack cholic acid extrusion activity. Cholic acid was accumulated spontaneously, driven by the transmembrane proton gradient....
Koning, Sonja M., Elferink, Marieke G. L., Konings, Wil N., Driessen, Arnold J. M.
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different β-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (Km = 175 nM) and involves an...
Sakamoto, Kanta, Margolles, Abelardo, Van Veen, Hendrik W., Konings, Wil N.
Lactobacillus brevis is a major contaminant of spoiled beer. The organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. The hop...
Structural Features of the Glutamate Transporter Family
Slotboom, Dirk Jan, Konings, Wil N., Lolkema, Juke S.
Neuronal and glial glutamate transporters remove the excitatory neurotransmitter glutamate from the synaptic cleft and thus prevent neurotoxicity. The proteins belong to a large and widespread family...
Molecular Properties of Bacterial Multidrug Transporters
Putman, Monique, Van Veen, Hendrik W., Konings, Wil N.
One of the mechanisms that bacteria utilize to evade the toxic effects of antibiotics is the active extrusion of structurally unrelated drugs from the cell. Both intrinsic and acquired multidrug...
Van De Kamp, Mart, Pizzinini, Enrica, Vos, Arnold, Van Der Lende, Ted R., Schuurs, Theo A., Newbert, Roger W., ...
In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products...
Mechanistic Properties of the Two-Component Bacteriocin Lactococcin G
Moll, Gert, Hildeng-Hauge, Håvard, Nissen-Meyer, Jon, Nes, Ingolf F., Konings, Wil N., Driessen, Arnold J. M.
Lactococcin G is a bacteriocin whose activity depends on the complementary action of two peptides, termed α and β. Biologically active, synthetic lactococcin G was used to study the mode of action...
Physiological Response of Lactobacillus plantarum to Salt and Nonelectrolyte Stress
Glaasker, Erwin, Tjan, Frans S. B., Ter Steeg, Pieter F., Konings, Wil N., Poolman, Bert
In this report, we compared the effects on the growth of Lactobacillus plantarum of raising the medium molarity by high concentrations of KCl or NaCl and iso-osmotic concentrations of nonionic...
Glaasker, Erwin, Konings, Wil N., Poolman, Bert
The accumulation of quaternary ammonium compounds in Lactobacillus plantarum is mediated via a single transport system with a high affinity for glycine betaine (apparent Km of 18 μM) and carnitine...
The Lantibiotic Nisin Induces Transmembrane Movement of a Fluorescent Phospholipid
Moll, Gert N., Konings, Wil N., Driessen, Arnold J. M.
Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid...
Fang, Gang, Konings, Wil N., Poolman, Bert
The peptide transport protein DtpT of Lactococcus lactis was purified and reconstituted into detergent-destabilized liposomes. The kinetics and substrate specificity of the transporter in the...
Membrane-Bound ATPase Contributes to Hop Resistance of Lactobacillus brevis
Sakamoto, Kanta, Van Veen, H. W., Saito, Hiromi, Kobayashi, Hiroshi, Konings, Wil N.
The activity of the membrane-bound H+-ATPase of the beer spoilage bacterium Lactobacillus brevis ABBC45 increased upon adaptation to bacteriostatic hop compounds. The ATPase activity was optimal...
Autoproteolysis of the Extracellular Serine Proteinase of Lactococcus lactis subsp. cremoris Wg2
The molecular masses of purified extracellular serine proteinase of a number of Lactococcus lactis strains vary significantly, and these molecular mass values do not correspond to the values...
Winters, David A., Poolman, Bert, Hemme, Denis, Konings, Wil N.
Membrane vesicles of Leuconostoc mesenteroides subsp. dextranicum fused with proteoliposomes prepared from Escherichia coli phospholipids containing beef heart cytochrome c oxidase were used to study...
Purification and Characterization of a Tripeptidase from Lactococcus lactis subsp. cremoris Wg2
Bosman, Boukje W., Tan, Paris S. T., Konings, Wil N.
A tripeptidase from a cell extract of Lactococcus lactis subsp. cremoris Wg2 has been purified to homogeneity by DEAE-Sephacel and phenyl-Sepharose chromatography followed by gel filtration over a...
Localization of Peptidases in Lactococci
Tan, Paris S. T., Chapot-Chartier, Marie-Pierre, Pos, Klaas M., Rousseau, Micheline, Boquien, Clair-Yves, Gripon, Jean-Claude, ...
The localization of two aminopeptidases, an X-prolyl-dipeptidyl aminopeptidase, an endopeptidase, and a tripeptidase in Lactococcus lactis was studied. Polyclonal antibodies raised against each...
Laan, Harry, Smid, Eddy J., De Leij, Loe, Schwander, Edwin, Konings, Wil N.
Twelve monoclonal antibodies directed to the cell-wall-associated proteinase of Lactococcus lactis subsp. cremoris Wg2 were isolated after immunization of BALB/c mice with a partially purified...
Mechanism of Proteinase Release from Lactococcus lactis subsp. cremoris Wg2
The procedure generally used for the isolation of extracellular, cell-associated proteinases of Lactococcus lactis species is based on the release of the proteinases by repeated incubation and...
Hugenholtz, Jeroen, Veldkamp, Hans, Konings, Wil N.
Antisera against four different strains of Streptococcus cremoris were raised by injecting rabbits with washed suspensions of whole cells. These antisera interacted specifically with the...
Selection of Protease-Positive and Protease-Negative Variants of Streptococcus cremoris
Hugenholtz, Jeroen, Splint, Rob, Konings, Wil N., Veldkamp, Hans
Protease-negative variants were shown to outcompete the wild-type strains of Streptococcus cremoris E8, HP, and Wg2 at pH values higher than 6.0 in milk. For S. cremoris E8 this process was studied...
Cell Wall-Associated Proteases of Streptococcus cremoris Wg2
Hugenholtz, Jeroen, Van Sinderen, Douwe, Kok, Jan, Konings, Wil N.
Two components of the proteolytic system, proteins A and B (J. Hugenholtz, F. Exterkate, and W. N. Konings, Appl. Environ. Microbiol. 48:1105-1110, 1984), have been studied in Streptococcus cremoris...
Mierau, Igor, Tan, Paris S. T., Haandrikman, Alfred J., Mayo, Balthasar, Kok, Jan, Leenhouts, Kees J., ...
Van Veen, Hendrik W., Margolles, Abelardo, Müller, Michael, Higgins, Christopher F., Konings, Wil N.
The bacterial LmrA protein and the mammalian multidrug resistance P-glycoprotein are closely related ATP-binding cassette (ABC) transporters that confer multidrug resistance on cells by mediating the...
Energy Supply for Active Transport in Anaerobically Grown Escherichia coli
Boonstra, Johannes, Downie, J. Allan, Konings, Wil N.
Escherichia coli K-12, grown under anaerobic conditions with glucose as the sole source of carbon and energy without any terminal electron acceptor added, contains a fumarate reductase system in...
Energetics of Leucyl-Leucine Hydrolysis in Streptococcus cremoris Wg2
Van Boven, Aart, Konings, Wil N.
The hydrolysis of the dipeptide leucyl-leucine by whole cells of Streptococcus cremoris Wg2 was dependent on the presence of the energy source lactose. Incubation of cells with uncouplers or ATPase...
The Proteolytic Systems of Streptococcus cremoris: an Immunological Analysis
Hugenholtz, Jeroen, Exterkate, Fred, Konings, Wil N.
The cell-wall-associated proteolytic systems of several Streptococcus cremoris strains were analyzed by crossed immunoelectrophoresis. At least four immunologically different components of the...
Lanfermeijer, Frank C., Detmers, Frank J.M., Konings, Wil N., Poolman, Bert
Lactococcus lactis degrades exogenous proteins such as β-casein to peptides of 4–30 amino acids, and uses these as nitrogen sources. The binding protein or receptor (OppALl) of the oligopeptide...
Lubelski, Jacek, Konings, Wil N., Driessen, Arnold J. M.
Summary: Membrane proteins responsible for the active efflux of structurally and functionally unrelated drugs were first characterized in higher eukaryotes. To date, a vast number of transporters...
Koning, Sonja M., Konings, Wil N., Driessen, Arnold J.M.
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different carbohydrates, such as starch, maltose and trehalose. Uptake of α-glucosides is mediated by two different, binding...