Wilhelm Ansorge

A Neutral Model of Transcriptome Evolution (2004)

Philipp Khaitovich, Gunter Weiss, Michael Lachmann, Ines Hellmann, Wolfgang Enard, Bjoern Muetzel, ...

Analysis of differences in gene expression between primate species suggests that the majority of them are selectively neutral and likely to have little or no functional consequences.

A Neutral Model of Transcriptome Evolution (2004)

Philipp Khaitovich, Gunter Weiss, Michael Lachmann, Ines Hellmann, Wolfgang Enard, Bjoern Muetzel, ...

Microarray technologies allow the identification of large numbers of expression differences within and between species. Although environmental and physiological stimuli are clearly responsible for...

A Neutral Model of Transcriptome Evolution (2004)

Khaitovich, Phillip, Weiss, Gunter, Lachmann, Michael, Hellmann, Ines, Enard, Wolfgang, Muetzel, Bjoern, ...

Microarray technologies allow the identification of large numbers of expression differences within and between species. Although environmental and physiological stimuli are clearly responsible for...

Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling (2004)

Kristian Almstrup, Ute Wirkner, Jonathon Blake, Christian Schwager, Wilhelm Ansorge, ...

Carcinoma in situ (CIS) is the common precursor of histologically heterogeneous testicular germ cell tumors (TGCTs), which in recent decades have markedly increased and now are the most common...

Optimization of oligonucleotide-based DNA microarrays (2002)

Relógio, Angela, Schwager, Christian, Richter, Alexandra, Ansorge, Wilhelm, Valcárcel, Juan

Oligonucleotide-based DNA microarrays are becoming increasingly useful for the analysis of gene expression and single nucleotide polymorphisms. Here we report a systematic study of the sensitivity,...

Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA (1989)

Voss, Hartmut, Schwager, Christian, Wirkner, Ute, Sproat, Brian, Zimmermann, Jurgen, Rosenthal, Andre, ...

In vitro amplification of genomic DNA and total RNA, as well as recombinant DNA, using one fluorescently labelled and one unlabelled primer during amplification, together with on-line analysis of the...

The 89,000-Mr murine cytomegalovirus immediate-early protein stimulates c-fos expression and cellular DNA synthesis (1988)

Schickedanz, Jörg, Philipson, Lennart, Ansorge, Wilhelm, Pepperkok, Rainer, Klein, Rüdiger, Koszinowski, Ulrich H.

The immediate-early (IE) genes of murine cytomegalovirus (MCMV) are expressed in the absence of prior viral protein synthesis and regulate the transcription of MCMV early genes. The effect of MCMV IE...

Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis (1987)

Ansorge, Wilhelm, Sproat, Brian, Stegemann, Josef, Schwager, Christian, Zenke, Martin

A simple system has been designed enabling ultrasensitive on-line detection of fluorescently labelled macromolecules, e.g. nucleic acids, proteins and peptides during electrophoretic separations in...

Anopheles gambiae pilot gene discovery project: Identification of mosquito innate immunity genes from expressed sequence tags generated from immune-competent cell lines

Dimopoulos, George, Casavant, Thomas L., Chang, Shereen, Scheetz, Todd, Roberts, Chad, Donohue, Micca, ...

Together with AIDS and tuberculosis, malaria is at the top of the list of devastating infectious diseases. However, molecular genetic studies of its major vector, Anopheles gambiae, are still quite...

Dynamics of Gene Expression Revealed by Comparison of Serial Analysis of Gene Expression Transcript Profiles from Yeast Grown on Two Different Carbon SourcesD⃞

Kal, Arnoud J., Van Zonneveld, Anton Jan, Benes, Vladimir, Van Den Berg, Marlene, Koerkamp, Marian Groot, Albermann, Kaj, ...

We describe a genome-wide characterization of mRNA transcript levels in yeast grown on the fatty acid oleate, determined using Serial Analysis of Gene Expression (SAGE). Comparison of this SAGE...

Complex Functions of AP-1 Transcription Factors in Differentiation and Survival of PC12 Cells

Leppä, Sirpa, Eriksson, Minna, Saffrich, Rainer, Ansorge, Wilhelm, Bohmann, Dirk

c-Jun activation by mitogen-activated protein kinases has been implicated in various cellular signal responses. We investigated how JNK and c-Jun contribute to neuronal differentiation, cell...

Characterization of SRp46, a Novel Human SR Splicing Factor Encoded by a PR264/SC35 Retropseudogene

Soret, Johann, Gattoni, Renata, Guyon, Cécile, Sureau, Alain, Popielarz, Michel, Le Rouzic, Erwann, ...

The highly conserved SR family contains a growing number of phosphoproteins acting as both essential and alternative splicing factors. In this study, we have cloned human genomic and cDNA sequences...

Optimization of oligonucleotide-based DNA microarrays

Relógio, Angela, Schwager, Christian, Richter, Alexandra, Ansorge, Wilhelm, Valcárcel, Juan

Oligonucleotide-based DNA microarrays are becoming increasingly useful for the analysis of gene expression and single nucleotide polymorphisms. Here we report a systematic study of the sensitivity,...

The DEXD/H-box RNA helicase RHII/Gu is a co-factor for c-Jun-activated transcription

Westermarck, Jukka, Weiss, Carsten, Saffrich, Rainer, Kast, Jürgen, Musti, Anna-Maria, Wessely, Matthias, ...

Tandem affinity purification (TAP) and mass spectrometric peptide sequencing showed that the DEAD-box RNA helicase RHII/Gu is a functional interaction partner of c-Jun in human cells. The N-terminal...

Induction of cyclin E–cdk2 kinase activity, E2F-dependent transcription and cell growth by Myc are genetically separable events

Beier, Rudolf, Bürgin, Andrea, Kiermaier, Astrid, Fero, Matthew, Karsunky, Holger, Saffrich, Rainer, ...

The c-myc gene has been implicated in three distinct genetic programs regulating cell proliferation: control of cyclin E–cdk2 kinase activity, E2F-dependent transcription and cell growth. We have...

The Genexpress IMAGE Knowledge Base of the Human Brain Transcriptome: A Prototype Integrated Resource for Functional and Computational Genomics

Piétu, Geneviève, Mariage-Samson, Régine, Fayein, Nicole-Adeline, Matingou, Christiane, Eveno, Eric, Houlgatte, Rémi, ...

Expression profiles of 5058 human gene transcripts represented by an array of 7451 clones from the first IMAGE Consortium cDNA library from infant brain have been collected by semiquantitative...

The Genexpress IMAGE Knowledge Base of the Human Muscle Transcriptome: A Resource of Structural, Functional, and Positional Candidate Genes for Muscle Physiology and Pathologies

Piétu, Geneviève, Eveno, Eric, Soury-Segurens, Béatrice, Fayein, Nicole-Adeline, Mariage-Samson, Régine, Matingou, Christiane, ...

Sequence, gene mapping, and expression data corresponding to 910 genes transcribed in human skeletal muscle have been integrated to form the muscle module of the Genexpress IMAGE Knowledge Base....

Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs

Wiemann, Stefan, Weil, Bernd, Wellenreuther, Ruth, Gassenhuber, Johannes, Glassl, Sabine, Ansorge, Wilhelm, ...

With the complete human genomic sequence being unraveled, the focus will shift to gene identification and to the functional analysis of gene products. The generation of a set of cDNAs, both sequences...

A Neutral Model of Transcriptome Evolution

Khaitovich, Philipp, Weiss, Gunter, Lachmann, Michael, Hellmann, Ines, Enard, Wolfgang, Muetzel, Bjoern, ...

Microarray technologies allow the identification of large numbers of expression differences within and between species. Although environmental and physiological stimuli are clearly responsible for...

An estimate of large-scale sequencing accuracy

Hill, Fergal, Gemünd, Christine, Benes, Vladimir, Ansorge, Wilhelm, Gibson, Toby J.

The accuracy of large-scale DNA sequencing is difficult to estimate without redundant effort. We have found that the mobile genetic element IS10, a component of the transposon Tn10, has contaminated...

Point mutation of bacterial artificial chromosomes by ET recombination

Muyrers, Joep P.P., Zhang, Youming, Benes, Vladimir, Testa, Giuseppe, Ansorge, Wilhelm, Stewart, A. Francis

Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow...

A Member of a Gene Family on Xp22.3, VCX-A, Is Deleted in Patients with X-Linked Nonspecific Mental Retardation

Fukami, Maki, Kirsch, Stefan, Schiller, Simone, Richter, Alexandra, Benes, Vladimir, Franco, Brunella, ...

X-linked nonspecific mental retardation (MRX) has a frequency of 0.15% in the male population and is caused by defects in several different genes on the human X chromosome. Genotype-phenotype...

Anopheles gambiae pilot gene discovery project: Identification of mosquito innate immunity genes from expressed sequence tags generated from immune-competent cell lines

Dimopoulos, George, Casavant, Thomas L., Chang, Shereen, Scheetz, Todd, Roberts, Chad, Donohue, Micca, ...

Together with AIDS and tuberculosis, malaria is at the top of the list of devastating infectious diseases. However, molecular genetic studies of its major vector, Anopheles gambiae, are still quite...

Dynamics of Gene Expression Revealed by Comparison of Serial Analysis of Gene Expression Transcript Profiles from Yeast Grown on Two Different Carbon SourcesD⃞

Kal, Arnoud J., Van Zonneveld, Anton Jan, Benes, Vladimir, Van Den Berg, Marlene, Koerkamp, Marian Groot, Albermann, Kaj, ...

We describe a genome-wide characterization of mRNA transcript levels in yeast grown on the fatty acid oleate, determined using Serial Analysis of Gene Expression (SAGE). Comparison of this SAGE...

Complex Functions of AP-1 Transcription Factors in Differentiation and Survival of PC12 Cells

Leppä, Sirpa, Eriksson, Minna, Saffrich, Rainer, Ansorge, Wilhelm, Bohmann, Dirk

c-Jun activation by mitogen-activated protein kinases has been implicated in various cellular signal responses. We investigated how JNK and c-Jun contribute to neuronal differentiation, cell...

Characterization of SRp46, a Novel Human SR Splicing Factor Encoded by a PR264/SC35 Retropseudogene

Soret, Johann, Gattoni, Renata, Guyon, Cécile, Sureau, Alain, Popielarz, Michel, Le Rouzic, Erwann, ...

The highly conserved SR family contains a growing number of phosphoproteins acting as both essential and alternative splicing factors. In this study, we have cloned human genomic and cDNA sequences...

Optimization of oligonucleotide-based DNA microarrays

Relógio, Angela, Schwager, Christian, Richter, Alexandra, Ansorge, Wilhelm, Valcárcel, Juan

Oligonucleotide-based DNA microarrays are becoming increasingly useful for the analysis of gene expression and single nucleotide polymorphisms. Here we report a systematic study of the sensitivity,...

The DEXD/H-box RNA helicase RHII/Gu is a co-factor for c-Jun-activated transcription

Westermarck, Jukka, Weiss, Carsten, Saffrich, Rainer, Kast, Jürgen, Musti, Anna-Maria, Wessely, Matthias, ...

Tandem affinity purification (TAP) and mass spectrometric peptide sequencing showed that the DEAD-box RNA helicase RHII/Gu is a functional interaction partner of c-Jun in human cells. The N-terminal...

Induction of cyclin E–cdk2 kinase activity, E2F-dependent transcription and cell growth by Myc are genetically separable events

Beier, Rudolf, Bürgin, Andrea, Kiermaier, Astrid, Fero, Matthew, Karsunky, Holger, Saffrich, Rainer, ...

The c-myc gene has been implicated in three distinct genetic programs regulating cell proliferation: control of cyclin E–cdk2 kinase activity, E2F-dependent transcription and cell growth. We have...

The Genexpress IMAGE Knowledge Base of the Human Brain Transcriptome: A Prototype Integrated Resource for Functional and Computational Genomics

Piétu, Geneviève, Mariage-Samson, Régine, Fayein, Nicole-Adeline, Matingou, Christiane, Eveno, Eric, Houlgatte, Rémi, ...

Expression profiles of 5058 human gene transcripts represented by an array of 7451 clones from the first IMAGE Consortium cDNA library from infant brain have been collected by semiquantitative...

The Genexpress IMAGE Knowledge Base of the Human Muscle Transcriptome: A Resource of Structural, Functional, and Positional Candidate Genes for Muscle Physiology and Pathologies

Piétu, Geneviève, Eveno, Eric, Soury-Segurens, Béatrice, Fayein, Nicole-Adeline, Mariage-Samson, Régine, Matingou, Christiane, ...

Sequence, gene mapping, and expression data corresponding to 910 genes transcribed in human skeletal muscle have been integrated to form the muscle module of the Genexpress IMAGE Knowledge Base....

Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs

Wiemann, Stefan, Weil, Bernd, Wellenreuther, Ruth, Gassenhuber, Johannes, Glassl, Sabine, Ansorge, Wilhelm, ...

With the complete human genomic sequence being unraveled, the focus will shift to gene identification and to the functional analysis of gene products. The generation of a set of cDNAs, both sequences...

A Neutral Model of Transcriptome Evolution

Khaitovich, Philipp, Weiss, Gunter, Lachmann, Michael, Hellmann, Ines, Enard, Wolfgang, Muetzel, Bjoern, ...

Microarray technologies allow the identification of large numbers of expression differences within and between species. Although environmental and physiological stimuli are clearly responsible for...

An estimate of large-scale sequencing accuracy

Hill, Fergal, Gemünd, Christine, Benes, Vladimir, Ansorge, Wilhelm, Gibson, Toby J.

The accuracy of large-scale DNA sequencing is difficult to estimate without redundant effort. We have found that the mobile genetic element IS10, a component of the transposon Tn10, has contaminated...

Point mutation of bacterial artificial chromosomes by ET recombination

Muyrers, Joep P.P., Zhang, Youming, Benes, Vladimir, Testa, Giuseppe, Ansorge, Wilhelm, Stewart, A. Francis

Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow...

A Member of a Gene Family on Xp22.3, VCX-A, Is Deleted in Patients with X-Linked Nonspecific Mental Retardation

Fukami, Maki, Kirsch, Stefan, Schiller, Simone, Richter, Alexandra, Benes, Vladimir, Franco, Brunella, ...

X-linked nonspecific mental retardation (MRX) has a frequency of 0.15% in the male population and is caused by defects in several different genes on the human X chromosome. Genotype-phenotype...

Microinjection of Anti-coilin Antibodies Affects the Structure of Coiled Bodies

Almeida, Fátima, Saffrich, Rainer, Ansorge, Wilhelm, Carmo-Fonseca, Maria

The coiled body is a distinct subnuclear domain enriched in small nuclear ribonucleoprotein particles (snRNPs) involved in processing of pre-mRNA. Although the function of the coiled body is still...