Fatty Acid Synthase: A Metabolic Enzyme and Candidate Oncogene in Prostate Cancer (2009)
Migita, Toshiro, Ruiz, Stacey, Fornari, Alessandro, Fiorentino, Michelangelo, Priolo, Carmen, Zadra, Giorgia, ...
Background Overexpression of the fatty acid synthase (FASN) gene has been implicated in prostate carcinogenesis. We sought to directly assess the oncogenic potential of FASN. Methods We used...
Bladder cancer: modeling and translation (2009)
Rosenberg, Jonathan E., Hahn, William C.
Transitional cell carcinoma of the bladder is a common malignancy worldwide that is associated with significant morbidity and mortality. Although superficial tumors can often be treated effectively,...
Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1 (2009)
Barbie, David A., Tamayo, Pablo, Boehm, Jesse S., Kim, So Young, Moody, Susan E., Dunn, Ian F., ...
Sonam Dolma, Stephen L. Lessnick, William C. Hahn, Brent R. Stockwell
Identification of genotype-selective antitumor agents using synthetic lethal chemical screening in engineered human
Malignant astrocytic glioma: genetics, biology, and paths to treatment (2007)
Furnari, Frank B., Fenton, Tim, Bachoo, Robert M., Mukasa, Akitake, Stommel, Jayne M., Stegh, Alexander, ...
Malignant astrocytic gliomas such as glioblastoma are the most common and lethal intracranial tumors. These cancers exhibit a relentless malignant progression characterized by widespread invasion...
Structural Basis of PP2A Inhibition by Small t Antigen (2007)
Uhn Soo Cho, Seamus Morrone, Anna A. Sablina, Jason D. Arroyo, William C. Hahn, Wenqing Xu
The SV40 small t antigen (ST) is a potent oncoprotein that perturbs the function of protein phosphatase 2A (PP2A). ST directly interacts with the PP2A scaffolding A subunit and alters PP2A activity...
Cancer genomics: integrating form and function (2007)
Kim, So Young, Hahn, William C.
The sequencing of the human genome has formed the foundation with which to develop technologies and reagents to perform true genome-scale biological studies. In particular, the development and...
Park, Yuhyun, Downing, Sean R., Kim, Dohyun, Hahn, William C., Li, Cheng, Kantoff, Philip W., ...
Motivation: Analysis of high-throughput proteomic/genomic data, in particular, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) data and microarray data,...
Minimizing the risk of reporting false positives in large-scale RNAi screens (2006)
Echeverri, Christophe J, Beachy, Philip A, Baum, Buzz, Boutros, Michael, Buchholz, Frank, Chanda, Sumit K, ...
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to...
Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants (2005)
Heidi Greulich, Tzu-Hsiu Chen, Whei Feng, Pasi A. Jänne, James V. Alvarez, Mauro Zappaterra, ...
Different EGFR mutations are associated with lung cancer. All of the classes can transform fibroblasts and lung epithelial cells, most are sensitive to erlotinib and gefininib, but exon 20 mutations...
Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants (2005)
Heidi Greulich, Tzu-Hsiu Chen, Whei Feng, Pasi A. Jänne, James V. Alvarez, Mauro Zappaterra, ...
Background Somatic mutations in the kinase domain of the epidermal growth factor receptor tyrosine kinase gene EGFR are common in lung adenocarcinoma. The presence of mutations correlates with tumor...
Lentivirus-delivered stable gene silencing by RNAi in primary cells (2003)
STEWART, SHEILA A., DYKXHOORN, DEREK M., PALLISER, DEBORAH, MIZUNO, HANA, YU, EVAN Y., AN, DONG SUNG, ...
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis...
Elenbaas, Brian, Spirio, Lisa, Koerner, Frederick, Fleming, Mark D., Zimonjic, Drazen B., Donaher, Joana Liu, ...
In order to develop a better understanding of the molecular events that transform normal human prostate cells into prostate cancer, we have developed a system of cell transformation that permits the...
In order to develop a better understanding of the molecular events that transform normal human prostate cells into prostate cancer, we have developed a system of cell transformation that permits the...
To develop an understanding of the molecular events that transform normal human prostate cells into prostate cancer, we have developed a system of cell transformation that permits the creation of...
Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization
Counter, Christopher M., Hahn, William C., Wei, Wenyi, Caddle, Stephanie Dickinson, Beijersbergen, Roderick L., Lansdorp, Peter M., ...
The immortalization of human cells is a critical step during tumorigenesis. In vitro, normal human somatic cells must overcome two proliferative blockades, senescence and crisis, to become immortal....
Dickson, Mark A., Hahn, William C., Ino, Yasushi, Ronfard, Vincent, Wu, Jenny Y., Weinberg, Robert A., ...
Normal human cells exhibit a limited replicative life span in culture, eventually arresting growth by a process termed senescence. Progressive telomere shortening appears to trigger senescence in...
Telomerase contributes to tumorigenesis by a telomere length-independent mechanism
Stewart, Sheila A., Hahn, William C., O'Connor, Benjamin F., Banner, Elisa N., Lundberg, Ante S., Modha, Poonam, ...
Once immortalized, human cells are susceptible to transformation by introduction of an oncogene such as ras. Several lines of evidence now suggest that the maintenance of telomere length is a major...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
While it is clear that cancer arises from the accumulation of genetic mutations that endow the malignant cell with the properties of uncontrolled growth and proliferation, the precise combinations of...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
Rheinwald, James G., Hahn, William C., Ramsey, Matthew R., Wu, Jenny Y., Guo, Zongyou, Tsao, Hensin, ...
Rheinwald, James G., Hahn, William C., Ramsey, Matthew R., Wu, Jenny Y., Guo, Zongyou, Tsao, Hensin, ...
With increasing frequency during serial passage in culture, primary human keratinocytes express p16INK4A (p16) and undergo senescence arrest. Keratinocytes engineered to express hTERT maintain long...
Johnson, F.Brad, Marciniak, Robert A., McVey, Mitch, Stewart, Sheila A., Hahn, William C., Guarente, Leonard
Werner syndrome (WS) is marked by early onset of features resembling aging, and is caused by loss of the RecQ family DNA helicase WRN. Precisely how loss of WRN leads to the phenotypes of WS is...
Wei, Wenyi, Jobling, Wendy A., Chen, Wen, Hahn, William C., Sedivy, John M.
Human cells are more resistant to both immortalization and malignant transformation than rodent cells. Recent studies have established the basic genetic requirements for the transformation of human...
Opitz, Oliver G., Suliman, Yasir, Hahn, William C., Harada, Hideki, Blum, Hubert E., Rustgi, Anil K.
The immortalization of human cells is a critical step in multistep carcinogenesis. Oral-esophageal carcinomas, a model system to investigate molecular mechanisms underlying squamous carcinogenesis,...
Chiang, Y. Jeffrey, Hemann, Michael T., Hathcock, Karen S., Tessarollo, Lino, Feigenbaum, Lionel, Hahn, William C., ...
Telomerase consists of two essential components, the telomerase RNA template (TR) and telomerase reverse transcriptase (TERT). The haplo-insufficiency of TR was recently shown to cause one form of...
The telomerase reverse transcriptase regulates chromatin state and DNA damage responses
Masutomi, Kenkichi, Possemato, Richard, Wong, Judy M. Y., Currier, Jennifer L., Tothova, Zuzana, Manola, Judith B., ...
Constitutive expression of telomerase prevents senescence and crisis by maintaining telomere homeostasis. However, recent evidence suggests that telomerase is dynamically regulated in normal cells...
Transformation of Human and Murine Fibroblasts without Viral Oncoproteins
Boehm, Jesse S., Hession, Meghan T., Bulmer, Sara E., Hahn, William C.
Murine embryo fibroblasts are readily transformed by the introduction of specific combinations of oncogenes; however, the expression of those same oncogenes in human cells fails to convert such cells...
Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants
Greulich, Heidi, Chen, Tzu-Hsiu, Feng, Whei, Jänne, Pasi A, Alvarez, James V, Zappaterra, Mauro, ...
Different EGFR mutations are associated with lung cancer. All of the classes can transform fibroblasts and lung epithelial cells, most are sensitive to erlotinib and gefininib, but exon 20 mutations...
Lentivirus-delivered stable gene silencing by RNAi in primary cells
STEWART, SHEILA A., DYKXHOORN, DEREK M., PALLISER, DEBORAH, MIZUNO, HANA, YU, EVAN Y., AN, DONG SUNG, ...
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis...
Dolma, Sonam, Lessnick, Stephen L., Hahn, William C., Stockwell, Brent R.
We used synthetic lethal high-throughput screening to interrogate 23,550 compounds for their ability to kill engineered tumorigenic cells but not their isogenic normal cell counterparts. We...
Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells
Elenbaas, Brian, Spirio, Lisa, Koerner, Frederick, Fleming, Mark D., Zimonjic, Drazen B., Donaher, Joana Liu, ...
A number of genetic mutations have been identified in human breast cancers, yet the specific combinations of mutations required in concert to form breast carcinoma cells remain unknown. One approach...
Creating oral squamous cancer cells: A cellular model of oral–esophageal carcinogenesis
Goessel, Gitta, Quante, Michael, Hahn, William C., Harada, Hideki, Heeg, Steffen, Suliman, Yasir, ...
Immortalization and malignant transformation are important steps in tumor development. The ability to induce these processes from normal human epithelial cells with genetic alterations frequently...
Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization
Counter, Christopher M., Hahn, William C., Wei, Wenyi, Caddle, Stephanie Dickinson, Beijersbergen, Roderick L., Lansdorp, Peter M., ...
The immortalization of human cells is a critical step during tumorigenesis. In vitro, normal human somatic cells must overcome two proliferative blockades, senescence and crisis, to become immortal....
Dickson, Mark A., Hahn, William C., Ino, Yasushi, Ronfard, Vincent, Wu, Jenny Y., Weinberg, Robert A., ...
Normal human cells exhibit a limited replicative life span in culture, eventually arresting growth by a process termed senescence. Progressive telomere shortening appears to trigger senescence in...
Telomerase contributes to tumorigenesis by a telomere length-independent mechanism
Stewart, Sheila A., Hahn, William C., O'Connor, Benjamin F., Banner, Elisa N., Lundberg, Ante S., Modha, Poonam, ...
Once immortalized, human cells are susceptible to transformation by introduction of an oncogene such as ras. Several lines of evidence now suggest that the maintenance of telomere length is a major...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
While it is clear that cancer arises from the accumulation of genetic mutations that endow the malignant cell with the properties of uncontrolled growth and proliferation, the precise combinations of...
Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation
Hahn, William C., Dessain, Scott K., Brooks, Mary W., King, Jessie E., Elenbaas, Brian, Sabatini, David M., ...
Rheinwald, James G., Hahn, William C., Ramsey, Matthew R., Wu, Jenny Y., Guo, Zongyou, Tsao, Hensin, ...
Rheinwald, James G., Hahn, William C., Ramsey, Matthew R., Wu, Jenny Y., Guo, Zongyou, Tsao, Hensin, ...
With increasing frequency during serial passage in culture, primary human keratinocytes express p16INK4A (p16) and undergo senescence arrest. Keratinocytes engineered to express hTERT maintain long...
Johnson, F.Brad, Marciniak, Robert A., McVey, Mitch, Stewart, Sheila A., Hahn, William C., Guarente, Leonard
Werner syndrome (WS) is marked by early onset of features resembling aging, and is caused by loss of the RecQ family DNA helicase WRN. Precisely how loss of WRN leads to the phenotypes of WS is...
Wei, Wenyi, Jobling, Wendy A., Chen, Wen, Hahn, William C., Sedivy, John M.
Human cells are more resistant to both immortalization and malignant transformation than rodent cells. Recent studies have established the basic genetic requirements for the transformation of human...
Opitz, Oliver G., Suliman, Yasir, Hahn, William C., Harada, Hideki, Blum, Hubert E., Rustgi, Anil K.
The immortalization of human cells is a critical step in multistep carcinogenesis. Oral-esophageal carcinomas, a model system to investigate molecular mechanisms underlying squamous carcinogenesis,...
Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells
Elenbaas, Brian, Spirio, Lisa, Koerner, Frederick, Fleming, Mark D., Zimonjic, Drazen B., Donaher, Joana Liu, ...
A number of genetic mutations have been identified in human breast cancers, yet the specific combinations of mutations required in concert to form breast carcinoma cells remain unknown. One approach...
Chiang, Y. Jeffrey, Hemann, Michael T., Hathcock, Karen S., Tessarollo, Lino, Feigenbaum, Lionel, Hahn, William C., ...
Telomerase consists of two essential components, the telomerase RNA template (TR) and telomerase reverse transcriptase (TERT). The haplo-insufficiency of TR was recently shown to cause one form of...
The telomerase reverse transcriptase regulates chromatin state and DNA damage responses
Masutomi, Kenkichi, Possemato, Richard, Wong, Judy M. Y., Currier, Jennifer L., Tothova, Zuzana, Manola, Judith B., ...
Constitutive expression of telomerase prevents senescence and crisis by maintaining telomere homeostasis. However, recent evidence suggests that telomerase is dynamically regulated in normal cells...
Transformation of Human and Murine Fibroblasts without Viral Oncoproteins
Boehm, Jesse S., Hession, Meghan T., Bulmer, Sara E., Hahn, William C.
Murine embryo fibroblasts are readily transformed by the introduction of specific combinations of oncogenes; however, the expression of those same oncogenes in human cells fails to convert such cells...
Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants
Greulich, Heidi, Chen, Tzu-Hsiu, Feng, Whei, Jänne, Pasi A, Alvarez, James V, Zappaterra, Mauro, ...
Different EGFR mutations are associated with lung cancer. All of the classes can transform fibroblasts and lung epithelial cells, most are sensitive to erlotinib and gefininib, but exon 20 mutations...
Creating oral squamous cancer cells: A cellular model of oral–esophageal carcinogenesis
Goessel, Gitta, Quante, Michael, Hahn, William C., Harada, Hideki, Heeg, Steffen, Suliman, Yasir, ...
Immortalization and malignant transformation are important steps in tumor development. The ability to induce these processes from normal human epithelial cells with genetic alterations frequently...
Lentivirus-delivered stable gene silencing by RNAi in primary cells
STEWART, SHEILA A., DYKXHOORN, DEREK M., PALLISER, DEBORAH, MIZUNO, HANA, YU, EVAN Y., AN, DONG SUNG, ...
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis...
Structural Basis of PP2A Inhibition by Small t Antigen
Cho, Uhn Soo, Morrone, Seamus, Sablina, Anna A, Arroyo, Jason D, Hahn, William C, Xu, Wenqing
The SV40 small t antigen (ST) is a potent oncoprotein that perturbs the function of protein phosphatase 2A (PP2A). ST directly interacts with the PP2A scaffolding A subunit and alters PP2A activity...
The SIRT1 Deacetylase Suppresses Intestinal Tumorigenesis and Colon Cancer Growth
Firestein, Ron, Blander, Gil, Michan, Shaday, Oberdoerffer, Philipp, Ogino, Shuji, Campbell, Jennifer, ...
Numerous longevity genes have been discovered in model organisms and altering their function results in prolonged lifespan. In mammals, some have speculated that any health benefits derived from...
Feedback Circuit among INK4 Tumor Suppressors Constrains Human Glioblastoma Development
Wiedemeyer, Ruprecht, Brennan, Cameron, Heffernan, Timothy P., Xiao, Yonghong, Mahoney, John, Protopopov, Alexei, ...
We have developed a nonheuristic genome topography scan (GTS) algorithm to characterize the patterns of genomic alterations in human glioblastoma (GBM), identifying frequent p18INK4C and p16INK4A...
BRAF Activation Initiates but Does Not Maintain Invasive Prostate Adenocarcinoma
Jeong, Joseph H., Wang, Zhenxiong, Guimaraes, Alexander S., Ouyang, Xuesong, Figueiredo, Jose L., Ding, Zhihu, ...
Prostate cancer is the second leading cause of cancer-related deaths in men. Activation of MAP kinase signaling pathway has been implicated in advanced and androgen-independent prostate cancers,...
Highly parallel identification of essential genes in cancer cells
Luo, Biao, Cheung, Hiu Wing, Subramanian, Aravind, Sharifnia, Tanaz, Okamoto, Michael, Yang, Xiaoping, ...
More complete knowledge of the molecular mechanisms underlying cancer will improve prevention, diagnosis and treatment. Efforts such as The Cancer Genome Atlas are systematically characterizing the...
Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer
Kimmelman, Alec C., Hezel, Aram F., Aguirre, Andrew J., Zheng, Hongwu, Paik, Ji-hye, Ying, Haoqiang, ...
Pancreas ductal adenocarcinoma (PDAC) is a highly lethal cancer that typically presents as advanced, unresectable disease. This invasive tendency, coupled with intrinsic resistance to standard...
The Role of PP2A A Subunits in Tumor Suppression
Sablina, Anna A, Hahn, William C
The protein phosphatase 2A (PP2A) family of heterotrimeric serine-threonine phosphatases participates in human cell transformation. Each functional PP2A complex contains one structural A subunit (Aα...