William Dowhan

Incapability of Utilizing Galactose by pgs1 Mutation Occurred on the Galactose Incorporation Step in Saccharomyces cerevisiae (2008)

Rho, Min-Suk, Su, Xuefeng, Lee, Yoon-Shik, Kim, Woo-Ho, Dowhan, William

A Saccharomyces cerevisiae pgs1 nulI mutant, which is deficient with phosphatidyl glycerol (PG) and cardiolipin (CL) biosynthesis, grows well on most fermentable carbon sources, but fails to grow on...

1999 Gordon Research Conference on Lipid Metabolism (1998)

Dowhan, William

The 1999 Gordon Research Conference on Molecular and Cellular Biology of Lipids was held June 27 - July 2 at Kimball Union Academy in Meriden, NH. The attendees represented a broad group of...

Molecular structure of the uvrC gene of Escherichia coli: identification of DNA sequences required for transcription of the uvrC gene (1982)

Sharma, Surendra, Dowhan, William, Moses, Robb E.

We have carried out experiments to identify the regulatory regions of the uvrC gene of Escherichia coli. A uvrC+ plasmid, pUV7, containing the intact transcriptional unit for the uvrC gene, was used...

The properties of D-serine dehydrase. (1969)

Dowhan, William.

Thesis (Ph. D. in Biochemistry)--University of California, Mar. 1969.

Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10-N-Nonyl Acridine Orange

Mileykovskaya, Eugenia, Dowhan, William

Cardiolipin (CL)-specific fluorescent dye 10-N-nonyl-acridine orange (NAO) was used to visualize CL distribution in Escherichia coli cells of different phospholipid compositions. In a filamentous...

Localization and Function of Early Cell Division Proteins in Filamentous Escherichia coli Cells Lacking Phosphatidylethanolamine

Mileykovskaya, Eugenia, Sun, Qin, Margolin, William, Dowhan, William

Escherichia coli cells that contain the pss-93 null mutation are completely deficient in the major membrane phospholipid phosphatidylethanolamine (PE). Such cells are defective in cell division. To...

A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition

Bogdanov, Mikhail, Heacock, Phillip N., Dowhan, William

To address the role of phospholipids in the topological organization of polytopic membrane proteins, the function and assembly of lactose permease (LacY) was studied in mutants of Escherichia coli...

Topology of polytopic membrane protein subdomains is dictated by membrane phospholipid composition

Wang, Xiaoyuan, Bogdanov, Mikhail, Dowhan, William

In Escherichia coli, the major cytoplasmic domain (C6) of the polytopic membrane protein lactose permease (LacY) is exposed to the opposite side of the membrane from a neighboring periplasmic domain...

Intracellular Distribution of Enzymes of Phospholipid Metabolism in Several Gram-Negative Bacteria

Dutt, Anuradha, Dowhan, William

Cell-free extracts of Salmonella typhimurium, Serratia marcescens, Enterobacter aerogenes, and Micrococcus cerificans contained the following enzymatic activities related to phospholipid metabolism:...

Translational Regulation of Nuclear Gene COX4 Expression by Mitochondrial Content of Phosphatidylglycerol and Cardiolipin in Saccharomyces cerevisiae

Su, Xuefeng, Dowhan, William

Previous results indicated that translation of four mitochondrion-encoded genes and one nucleus-encoded gene (COX4) is repressed in mutants (pgs1Δ) of Saccharomyces cerevisiae lacking...

Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10-N-Nonyl Acridine Orange

Mileykovskaya, Eugenia, Dowhan, William

Cardiolipin (CL)-specific fluorescent dye 10-N-nonyl-acridine orange (NAO) was used to visualize CL distribution in Escherichia coli cells of different phospholipid compositions. In a filamentous...

Localization and Function of Early Cell Division Proteins in Filamentous Escherichia coli Cells Lacking Phosphatidylethanolamine

Mileykovskaya, Eugenia, Sun, Qin, Margolin, William, Dowhan, William

Escherichia coli cells that contain the pss-93 null mutation are completely deficient in the major membrane phospholipid phosphatidylethanolamine (PE). Such cells are defective in cell division. To...

A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition

Bogdanov, Mikhail, Heacock, Phillip N., Dowhan, William

To address the role of phospholipids in the topological organization of polytopic membrane proteins, the function and assembly of lactose permease (LacY) was studied in mutants of Escherichia coli...

Topology of polytopic membrane protein subdomains is dictated by membrane phospholipid composition

Wang, Xiaoyuan, Bogdanov, Mikhail, Dowhan, William

In Escherichia coli, the major cytoplasmic domain (C6) of the polytopic membrane protein lactose permease (LacY) is exposed to the opposite side of the membrane from a neighboring periplasmic domain...

Intracellular Distribution of Enzymes of Phospholipid Metabolism in Several Gram-Negative Bacteria

Dutt, Anuradha, Dowhan, William

Cell-free extracts of Salmonella typhimurium, Serratia marcescens, Enterobacter aerogenes, and Micrococcus cerificans contained the following enzymatic activities related to phospholipid metabolism:...

Translational Regulation of Nuclear Gene COX4 Expression by Mitochondrial Content of Phosphatidylglycerol and Cardiolipin in Saccharomyces cerevisiae

Su, Xuefeng, Dowhan, William

Previous results indicated that translation of four mitochondrion-encoded genes and one nucleus-encoded gene (COX4) is repressed in mutants (pgs1Δ) of Saccharomyces cerevisiae lacking...

To flip or not to flip: lipid–protein charge interactions are a determinant of final membrane protein topology

Bogdanov, Mikhail, Xie, Jun, Heacock, Phil, Dowhan, William

The molecular details of how lipids influence final topological organization of membrane proteins are not well understood. Here, we present evidence that final topology is influenced by...

The translocator maintenance protein Tam41 is required for mitochondrial cardiolipin biosynthesis

Kutik, Stephan, Rissler, Michael, Guan, Xue Li, Guiard, Bernard, Shui, Guanghou, Gebert, Natalia, ...

The mitochondrial inner membrane contains different translocator systems for the import of presequence-carrying proteins and carrier proteins. The translocator assembly and maintenance protein 41...