Decreased Azithromycin Susceptibility of Neisseria gonorrhoeae Due to mtrR Mutations
Zarantonelli, Leticia, Borthagaray, Graciela, Lee, Eun-Hee, Shafer, William M.
Single-dose azithromycin therapy has recently been used in Uruguay for the treatment of uncomplicated gonococcal infections. As part of an active surveillance study to monitor the emergence of...
Snyder, Lori A. S., Saunders, Nigel J., Shafer, William M.
A cluster of 18 open reading frames (ORFs), 15 of which are homologous to genes involved in division and cell wall synthesis, has been identified in Neisseria gonorrhoeae and Neisseria meningitidis....
Rouquette-Loughlin, Corinne, Stojiljkovic, Igor, Hrobowski, Tara, Balthazar, Jacqueline T., Shafer, William M.
The MtrC-MtrD-MtrE efflux pump possessed by Neisseria gonorrhoeae is very similar to the MexA-MexB-OprM efflux pump of Pseudomonas aeruginosa. Because the antimicrobial resistance property afforded...
Veal, Wendy L., Nicholas, Robert A., Shafer, William M.
The importance of the mtrCDE-encoded efflux pump in conferring chromosomally mediated penicillin resistance on certain strains of Neisseria gonorrhoeae was determined by using genetic derivatives of...
Rouquette-Loughlin, Corinne, Dunham, Steven A., Kuhn, Michael, Balthazar, Jacqueline T., Shafer, William M.
In Neisseria gonorrhoeae and Neisseria meningitidis, we identified a gene that would encode a protein highly similar to NorM of Vibrio parahaemolyticus (Y. Morita et al., Antimicrob. Agents...
Katzif, Samuel, Danavall, Damien, Bowers, Samera, Balthazar, Jacqueline T., Shafer, William M.
A Tn551 insertional library of Staphylococcus aureus strain ISP479 was challenged with an antimicrobial peptide (CG 117-136) derived from human neutrophil cathepsin G (CG). After repeated selection...
Jerse, Ann E., Sharma, Nirmala D., Simms, Amy N., Crow, Emily T., Snyder, Lori A., Shafer, William M.
Active efflux of antimicrobial substances is likely to be an important bacterial defense against inhibitory host factors inherent to different body sites. Two well-characterized multidrug resistance...
Chen, Ching-ju, Tobiason, Deborah M., Thomas, Christopher E., Shafer, William M., Seifert, H. Steven, Sparling, P. Frederick
A spontaneous point mutation in pilQ (pilQ1) resulted in phenotypic suppression of a hemoglobin (Hb) receptor mutant (hpuAB mutant), allowing gonococci to grow on Hb as the sole source of iron. PilQ,...
Rivera-Marrero, Carlos A., Stewart, Julie, Shafer, William M., Roman, Jesse
Cathepsin G (CatG) is a serine protease found in the azurophilic granules of monocytes that is known to have antimicrobial properties, but its role in Mycobacterium tuberculosis infection is unknown....
Folster, Jason P., Shafer, William M.
The obligate human pathogen Neisseria gonorrhoeae uses the MtrC-MtrD-MtrE efflux pump to resist structurally diverse hydrophobic antimicrobial agents (HAs), some of which bathe mucosal surfaces that...
Characterization of the Multiple Transferable Resistance Repressor, MtrR, from Neisseria gonorrhoeae
Hoffmann, Katherine M., Williams, Daniel, Shafer, William M., Brennan, Richard G.
MtrR represses expression of the Neisseria gonorrhoeae mtrCDE multidrug efflux transporter genes. MtrR displays salt-dependent DNA binding, a stoichiometry of two dimers per DNA site, and, for a...
Cationic Antimicrobial Peptide Resistance in Neisseria meningitidis
Tzeng, Yih-Ling, Ambrose, Karita D., Zughaier, Susu, Zhou, Xiaoliu, Miller, Yoon K., Shafer, William M., ...
Cationic antimicrobial peptides (CAMPs) are important components of the innate host defense system against microbial infections and microbial products. However, the human pathogen Neisseria...
Rasmussen, Andrew W., Alexander, Heather L., Perkins-Balding, Donna, Shafer, William M., Stojiljkovic, Igor
Several genetic systems that allow the use of iron-protoporphyrin IX (heme) have been described for the pathogenic bacterium Neisseria meningitidis. However, many questions about the process of heme...
CspA Regulates Pigment Production in Staphylococcus aureus through a SigB-Dependent Mechanism
Katzif, Samuel, Lee, Eun-Hee, Law, Anthony B., Tzeng, Yih-Ling, Shafer, William M.
We report that the cold shock protein CspA of Staphylococcus aureus is required for maximal production of pigment. Results from transcriptional studies revealed that loss of CspA resulted in...
Zughaier, Susu M., Shafer, William M., Stephens, David S.
Antimicrobial peptides (AMPs), in addition to their antibacterial properties, are also chemotactic and signalling molecules that connect the innate and adaptive immune responses. The role of AMP [α...
Decreased Azithromycin Susceptibility of Neisseria gonorrhoeae Due to mtrR Mutations
Zarantonelli, Leticia, Borthagaray, Graciela, Lee, Eun-Hee, Shafer, William M.
Single-dose azithromycin therapy has recently been used in Uruguay for the treatment of uncomplicated gonococcal infections. As part of an active surveillance study to monitor the emergence of...
Snyder, Lori A. S., Saunders, Nigel J., Shafer, William M.
A cluster of 18 open reading frames (ORFs), 15 of which are homologous to genes involved in division and cell wall synthesis, has been identified in Neisseria gonorrhoeae and Neisseria meningitidis....
Rouquette-Loughlin, Corinne, Stojiljkovic, Igor, Hrobowski, Tara, Balthazar, Jacqueline T., Shafer, William M.
The MtrC-MtrD-MtrE efflux pump possessed by Neisseria gonorrhoeae is very similar to the MexA-MexB-OprM efflux pump of Pseudomonas aeruginosa. Because the antimicrobial resistance property afforded...
Veal, Wendy L., Nicholas, Robert A., Shafer, William M.
The importance of the mtrCDE-encoded efflux pump in conferring chromosomally mediated penicillin resistance on certain strains of Neisseria gonorrhoeae was determined by using genetic derivatives of...
Rouquette-Loughlin, Corinne, Dunham, Steven A., Kuhn, Michael, Balthazar, Jacqueline T., Shafer, William M.
In Neisseria gonorrhoeae and Neisseria meningitidis, we identified a gene that would encode a protein highly similar to NorM of Vibrio parahaemolyticus (Y. Morita et al., Antimicrob. Agents...
Katzif, Samuel, Danavall, Damien, Bowers, Samera, Balthazar, Jacqueline T., Shafer, William M.
A Tn551 insertional library of Staphylococcus aureus strain ISP479 was challenged with an antimicrobial peptide (CG 117-136) derived from human neutrophil cathepsin G (CG). After repeated selection...
Jerse, Ann E., Sharma, Nirmala D., Simms, Amy N., Crow, Emily T., Snyder, Lori A., Shafer, William M.
Active efflux of antimicrobial substances is likely to be an important bacterial defense against inhibitory host factors inherent to different body sites. Two well-characterized multidrug resistance...
Chen, Ching-ju, Tobiason, Deborah M., Thomas, Christopher E., Shafer, William M., Seifert, H. Steven, Sparling, P. Frederick
A spontaneous point mutation in pilQ (pilQ1) resulted in phenotypic suppression of a hemoglobin (Hb) receptor mutant (hpuAB mutant), allowing gonococci to grow on Hb as the sole source of iron. PilQ,...
Rivera-Marrero, Carlos A., Stewart, Julie, Shafer, William M., Roman, Jesse
Cathepsin G (CatG) is a serine protease found in the azurophilic granules of monocytes that is known to have antimicrobial properties, but its role in Mycobacterium tuberculosis infection is unknown....
Folster, Jason P., Shafer, William M.
The obligate human pathogen Neisseria gonorrhoeae uses the MtrC-MtrD-MtrE efflux pump to resist structurally diverse hydrophobic antimicrobial agents (HAs), some of which bathe mucosal surfaces that...
Characterization of the Multiple Transferable Resistance Repressor, MtrR, from Neisseria gonorrhoeae
Hoffmann, Katherine M., Williams, Daniel, Shafer, William M., Brennan, Richard G.
MtrR represses expression of the Neisseria gonorrhoeae mtrCDE multidrug efflux transporter genes. MtrR displays salt-dependent DNA binding, a stoichiometry of two dimers per DNA site, and, for a...
Cationic Antimicrobial Peptide Resistance in Neisseria meningitidis
Tzeng, Yih-Ling, Ambrose, Karita D., Zughaier, Susu, Zhou, Xiaoliu, Miller, Yoon K., Shafer, William M., ...
Cationic antimicrobial peptides (CAMPs) are important components of the innate host defense system against microbial infections and microbial products. However, the human pathogen Neisseria...
Rasmussen, Andrew W., Alexander, Heather L., Perkins-Balding, Donna, Shafer, William M., Stojiljkovic, Igor
Several genetic systems that allow the use of iron-protoporphyrin IX (heme) have been described for the pathogenic bacterium Neisseria meningitidis. However, many questions about the process of heme...
CspA Regulates Pigment Production in Staphylococcus aureus through a SigB-Dependent Mechanism
Katzif, Samuel, Lee, Eun-Hee, Law, Anthony B., Tzeng, Yih-Ling, Shafer, William M.
We report that the cold shock protein CspA of Staphylococcus aureus is required for maximal production of pigment. Results from transcriptional studies revealed that loss of CspA resulted in...
Folster, Jason P., Dhulipala, Vijaya, Nicholas, Robert A., Shafer, William M.
Neisseria gonorrhoeae utilizes the mtrCDE-encoded efflux pump system to resist not only host-derived, hydrophobic antimicrobials that bathe mucosal surfaces, which likely aids in its ability to...
Friedrich, Alexandra, Arvidson, Cindy G., Shafer, William M., Lee, Eun-Hee, So, Magdalene
Retraction of type IV pili is mediated by PilT. We show that loss of pilT function leads to upregulation of mtrF (multiple transferable resistance) and two operons encoding putative ABC transporters...
Kamal, Nazia, Rouquette-Loughlin, Corinne, Shafer, William M.
A 2.9-kilobase pair locus in Neisseria meningitidis was identified as containing transcriptionally linked open reading frames encoding TolC- and HlyD-like proteins. Although the meningococcal TolC...
Garvin, Lotisha E., Bash, Margaret C., Keys, Christine, Warner, Douglas M., Ram, Sanjay, Shafer, William M., ...
Typing of the porB variable region (VR) is an epidemiological tool that classifies gonococcal strains based on sequence differences in regions of the porB gene that encode surface-exposed loops. The...