Epitope tagging of endogenous genes in diverse human cell lines (2008)
Kim, Jung-Sik, Bonifant, Challice, Bunz, Fred, Lane, William S., Waldman, Todd
Epitope tagging is a powerful and commonly used approach for studying the physical properties of proteins and their functions and localization in eukaryotic cells. In the case of Saccharomyces...
Shaw, Bryan F., Schneider, Gregory F., Bilgiçer, Basar, Kaufman, George K., Neveu, John M., Lane, William S., ...
This paper reports that the acetylation of lysine ϵ-NH3 + groups of α-amylase—one of the most important hydrolytic enzymes used in industry—produces highly negatively charged variants that...
Functional relevance of novel p300-mediated lysine 314 and 315 acetylation of RelA/p65 (2008)
Buerki, Christine, Rothgiesser, Karin M., Valovka, Taras, Owen, Heather R., Rehrauer, Hubert, Fey, Monika, ...
Nuclear factor kappaB (NF-κB) plays an important role in the transcriptional regulation of genes involved in immunity and cell survival. We show here in vitro and in vivo acetylation of RelA/p65 by...
Nathan, Dafna, Ingvarsdottir, Kristin, Sterner, David E., Bylebyl, Gwendolyn R., Dokmanovic, Milos, Dorsey, Jean A., ...
Covalent histone post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitylation play pivotal roles in regulating many cellular processes, including...
Nathan, Dafna, Ingvarsdottir, Kristin, Sterner, David E., Bylebyl, Gwendolyn R., Dokmanovic, Milos, Dorsey, Jean A., ...
Covalent histone post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitylation play pivotal roles in regulating many cellular processes, including...
HIV-1 Tat interacts with LIS1 protein (2005)
Epie, Nicolas, Ammosova, Tatyana, Sapir, Tamar, Voloshin, Yaroslav, Lane, William S, Turner, Willie, ...
Abstract Background HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular...
Min Z. Levine, Patricia P. Wilkins, William S. Lane, John M. Asara, Kathy Hancock, ...
Adult and larval stages of Taenia solium cause 2 diseases in humans, i.e., taeniasis and cysticercosis, respectively. Diagnosis and treatment of taeniasis are the ultimate means to eliminate...
Sato, Shigeo, Tomomori-Sato, Chieri, Banks, Charles A. S., Sorokina, Irina, Parmely, Tari J., Kong, Stephanie E., ...
The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transactivators. We recently identified a mammalian homologue of yeast Mediator...
Yeast Enhancer of Polycomb defines global Esa1-dependent acetylation of chromatin (2003)
Boudreault, Alexandre A., Cronier, Dominique, Selleck, William, Lacoste, Nicolas, Utley, Rhea T., Allard, Stéphane, ...
Drosophila Enhancer of Polycomb, E(Pc), is a suppressor of position-effect variegation and an enhancer of both Polycomb and trithorax mutations. A homologous yeast protein, Epl1, is a subunit of the...
Cantor, Sharon B., Bell, Daphne W., Ganesan, Shridar, Kass, Elizabeth M., Drapkin, Ronny, Grossman, Steven R., ...
BRCA1 interacts in vivo with a novel protein, BACH1, a member of the DEAH helicase family. BACH1 binds directly to the BRCT repeats of BRCA1. A BACH1 derivative, bearing a mutation in a residue that...
Guenther, Matthew G., Lane, William S., Fischle, Wolfgang, Verdin, Eric, Lazar, Mitchell A., Shiekhattar, Ramin
A protein phosphatase functions to recycle RNA polymerase II (1999)
Cho, Helen, Kim, Tae-Kyung, Mancebo, Helena, Lane, William S., Flores, Osvaldo, Reinberg, Danny
Association of acyl-CoA synthetase-1 with GLUT4-containing vesicles (1998)
Sleeman, Mark W., Donegan, Niles P., Heller-Harrison, Robin A., Lane, William S., Czech, Michael P.
GLUT4, the glucose transporter present in insulin-sensitive tissues, resides in intracellular vesicular structures and translocates to the cell surface in response to insulin. In an attempt to...
Identification of an early endosomal protein regulated by phosphatidylinositol 3-kinase (1997)
Patki, Varsha, Virbasius, Joseph V., Lane, William S., Toh, Ban-Hock, Shpetner, Howard S., Corvera, Silvia
Phosphatidylinositol 3-kinases (PI 3-kinases) have been implicated in membrane trafficking in the secretory and endocytic pathways of yeast and mammalian cells, but the molecular mechanisms by which...
Chicz, Roman M., Lane, William S., Robinson, Renée A., Trucco, Massimo, Strominger, Jack L., Gorga, Joan C.
Genetic susceptibility to several autoimmune disorders is associated with the expression of certain MHC class II alleles. Insight into the etiology of such diseases awaits the identification of the...
Sander, Miriam, Lowenhaupt, Ky, Lane, William S., Rich, Alexander
We previously reported the purification of a protein from Drosophila embryo extracts that carries out the strand transfer step in homologous recombination (Lowenhaupt, K., Sander, M., Hauser, C. and...
Troponin I is present in human cartilage and inhibits angiogenesis
Moses, Marsha A., Wiederschain, Dmitri, Wu, Inmin, Fernandez, Cecilia A., Ghazizadeh, Vahid, Lane, William S., ...
Cartilage is an avascular and relatively tumor-resistant tissue. Work from a number of laboratories, including our own, has demonstrated that cartilage is an enriched source of endogenous inhibitors...
Sylla, Bakary S., Murphy, Kerry, Cahir-McFarland, Ellen, Lane, William S., Mosialos, George, Kieff, Elliott
The X-linked lymphoproliferative syndrome (XLP) is a genetic disorder in which affected males have a morbid or fatal response to Epstein–Barr virus infection. The XLP deficiency has been mapped to...
Palfi, Zsofia, Lücke, Stephan, Lahm, Hans-Werner, Lane, William S., Kruft, Volker, Bragado-Nilsson, Elisabeth, ...
Each of the trypanosome small nuclear ribonucleoproteins (snRNPs) U2, U4/U6, and U5, as well as the spliced leader (SL) RNP, contains a core of common proteins, which we have previously identified....
Ouyang, Ying-bin, Lane, William S., Moore, Kevin L.
Tyrosine O-sulfation is a common posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called tyrosylprotein sulfotransferase...
Identification of an early endosomal protein regulated by phosphatidylinositol 3-kinase
Patki, Varsha, Virbasius, Joe, Lane, William S., Toh, Ban-Hock, Shpetner, Howard S., Corvera, Silvia
Phosphatidylinositol 3-kinases (PI 3-kinases) have been implicated in membrane trafficking in the secretory and endocytic pathways of yeast and mammalian cells, but the molecular mechanisms by which...
Lai, Albert, Kennedy, Brian K., Barbie, David A., Bertos, Nicholas R., Yang, Xiang Jiao, Theberge, Marie-Christine, ...
Retinoblastoma (RB) tumor suppressor family pocket proteins induce cell cycle arrest by repressing transcription of E2F-regulated genes through both histone deacetylase (HDAC)-dependent and...
Nourani, Amine, Doyon, Yannick, Utley, Rhea T., Allard, Stéphane, Lane, William S., Côté, Jacques
The yeast NuA4 complex is a histone H4 and H2A acetyltransferase involved in transcription regulation and essential for cell cycle progression. We identify here a novel subunit of the complex, Yng2p,...
DNA-Dependent Protein Kinase Phosphorylation of IκBα and IκBβ Regulates NF-κB DNA Binding Properties
Liu, Li, Kwak, Youn-Tae, Bex, Françoise, García-Martínez, León F., Li, Xiao-Hua, Meek, Katheryn, ...
Regulation of the IκBα and IκBβ proteins is critical for modulating NF-κB-directed gene expression. Both IκBα and IκBβ are substrates for cellular kinases that phosphorylate the amino and...
Han, Sang Jun, Lee, Young Chul, Gim, Byung Soo, Ryu, Gi-Hyuck, Park, Soon Jung, Lane, William S., ...
The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription. The Mediator complex is composed of two subcomplexes, the Rgr1 and Srb4...
Human Elongator facilitates RNA polymerase II transcription through chromatin
Kim, Jae-Hyun, Lane, William S., Reinberg, Danny
A human Elongator complex was purified from HeLa cells and found to be composed of three polypeptides. Human Elongator contains histone acetyltransferase activity with specificity to histone H3 and,...
A core–BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes
Hakimi, Mohamed-Ali, Bochar, Daniel A., Chenoweth, Josh, Lane, William S., Mandel, Gail, Shiekhattar, Ramin
BRAF35, a structural DNA-binding protein, initially was identified as a component of a large BRCA2-containing complex. Biochemical analysis revealed the presence of a smaller core–BRAF35 complex...
Brower, Christopher S., Sato, Shigeo, Tomomori-Sato, Chieri, Kamura, Takumi, Pause, Arnim, Stearman, Robert, ...
The heterodimeric Elongin BC complex has been shown to interact in vitro and in cells with a conserved BC-box motif found in an increasing number of proteins including RNA polymerase II elongation...
Friedl, Erika M., Lane, William S., Erdjument-Bromage, Hediye, Tempst, Paul, Reinberg, Danny
The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAPII) is heavily phosphorylated during the transition from transcription initiation to the establishment of an...
Yasothornsrikul, Sukkid, Greenbaum, Doron, Medzihradszky, Katalin F., Toneff, Thomas, Bundey, Richard, Miller, Ruthellen, ...
Multistep proteolytic mechanisms are essential for converting proprotein precursors into active peptide neurotransmitters and hormones. Cysteine proteases have been implicated in the processing of...
Functional Analysis of the Subunits of the Chromatin Assembly Factor RSF
Loyola, Alejandra, Huang, Jing-Yi, LeRoy, Gary, Hu, Sherrie, Wang, Yuh-Hwa, Donnelly, Robert J., ...
The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed...
Yeast Enhancer of Polycomb defines global Esa1-dependent acetylation of chromatin
Boudreault, Alexandre A., Cronier, Dominique, Selleck, William, Lacoste, Nicolas, Utley, Rhea T., Allard, Stéphane, ...
Drosophila Enhancer of Polycomb, E(Pc), is a suppressor of position-effect variegation and an enhancer of both Polycomb and trithorax mutations. A homologous yeast protein, Epl1, is a subunit of the...
Isolation of human NURF: a regulator of Engrailed gene expression
Barak, Orr, Lazzaro, Maribeth A., Lane, William S., Speicher, David W., Picketts, David J., Shiekhattar, Ramin
The modification of chromatin structure is an important regulatory mechanism for developmental gene expression. Differential expression of the mammalian ISWI genes, SNF2H and SNF2L, has suggested...
Kuo, Alfred, Zhong, Cuiling, Lane, William S., Derynck, Rik
Transforming growth factor-α (TGF-α) and related proteins represent a family of transmembrane growth factors with representatives in flies and worms. Little is known about the transport of TGF-α...
A core SMRT corepressor complex containing HDAC3 and TBL1, a WD40-repeat protein linked to deafness
Guenther, Matthew G., Lane, William S., Fischle, Wolfgang, Verdin, Eric, Lazar, Mitchell A., Shiekhattar, Ramin
The corepressor SMRT mediates repression by thyroid hormone receptor (TR) as well as other nuclear hormone receptors and transcription factors. Here we report the isolation of a novel SMRT-containing...
A protein phosphatase functions to recycle RNA polymerase II
Cho, Helen, Kim, Tae-Kyung, Mancebo, Helena, Lane, William S., Flores, Osvaldo, Reinberg, Danny
Transcription is regulated by the state of phosphorylation of a heptapeptide repeat known as the carboxy-terminal domain (CTD) present in the largest subunit of RNA polymerase II (RNAPII). RNAPII...
Chamberlain, Joel R., Lee, Yoon, Lane, William S., Engelke, David R.
Ribonuclease P (RNase P) is a ribonucleoprotein enzyme that cleaves precursor tRNA transcripts to give mature 5′ ends. RNase P in eubacteria has a large, catalytic RNA subunit and a small protein...
Doyon, Yannick, Selleck, William, Lane, William S., Tan, Song, Côté, Jacques
The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell...
The c-MYC Oncoprotein Is a Substrate of the Acetyltransferases hGCN5/PCAF and TIP60
Patel, Jagruti H., Du, Yanping, Ard, Penny G., Phillips, Charles, Carella, Beth, Chen, Chi-Ju, ...
The c-MYC oncoprotein functions as a sequence-specific transcription factor. The ability of c-MYC to activate transcription relies in part on the recruitment of cofactor complexes containing the...
Phosphorylation Site Mutations Affect Herpes Simplex Virus Type 1 ICP0 Function
Davido, David J., Von Zagorski, William F., Lane, William S., Schaffer, Priscilla A.
The herpes simplex virus type 1 (HSV-1) immediate-early (IE) regulatory protein infected-cell protein 0 (ICP0) is a strong and global transactivator of both viral and cellular genes. In a previous...
HIV-1 Tat interacts with LIS1 protein
Epie, Nicolas, Ammosova, Tatyana, Sapir, Tamar, Voloshin, Yaroslav, Lane, William S, Turner, Willie, ...
Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress
Guettouche, Toumy, Boellmann, Frank, Lane, William S, Voellmy, Richard
Nekhai, Sergei, Zhou, Meisheng, Fernandez, Anne, Lane, William S, Lamb, Ned J C, Brady, John, ...
HIV-1 gene expression is regulated by a viral transactivator protein (Tat) which induces transcriptional elongation of HIV-1 long tandem repeat (LTR). This induction requires hyperphosphorylation of...
Smith, Edwin R., Cayrou, Christelle, Huang, Rong, Lane, William S., Côté, Jacques, Lucchesi, John C.
We describe a stable, multisubunit human histone acetyltransferase complex (hMSL) that contains homologs of the Drosophila dosage compensation proteins MOF, MSL1, MSL2, and MSL3. This complex shows...
ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage
Chen, Lihong, Gilkes, Daniele M, Pan, Yu, Lane, William S, Chen, Jiandong
The p53 tumor suppressor is activated after DNA damage to maintain genomic stability and prevent transformation. Rapid activation of p53 by ionizing radiation is dependent on signaling by the ATM...
Smith, Edwin R., Cayrou, Christelle, Huang, Rong, Lane, William S., Côté, Jacques, Lucchesi, John C.
Stockinger, Walter, Zhang, Shao C., Trivedi, Vishal, Jarzylo, Larissa A., Shieh, Eugenie C., Lane, William S., ...
Fusion of phagosomes with late endocytic organelles is essential for cellular digestion of microbial pathogens, senescent cells, apoptotic bodies, and retinal outer segment fragments. To further...
Nathan, Dafna, Ingvarsdottir, Kristin, Sterner, David E., Bylebyl, Gwendolyn R., Dokmanovic, Milos, Dorsey, Jean A., ...
Covalent histone post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitylation play pivotal roles in regulating many cellular processes, including...
Sm core variation in spliceosomal small nuclear ribonucleoproteins from Trypanosoma brucei
Wang, Pingping, Palfi, Zsofia, Preusser, Christian, Lücke, Stephan, Lane, William S, Kambach, Christian, ...
Messenger RNA processing in trypanosomes by cis and trans splicing requires spliceosomal small nuclear ribonucleoproteins (snRNPs) U1, U2, U4/U6, and U5, as well as the spliced leader (SL) RNP. As in...
Functional relevance of novel p300-mediated lysine 314 and 315 acetylation of RelA/p65
Buerki, Christine, Rothgiesser, Karin M., Valovka, Taras, Owen, Heather R., Rehrauer, Hubert, Fey, Monika, ...
Nuclear factor kappaB (NF-κB) plays an important role in the transcriptional regulation of genes involved in immunity and cell survival. We show here in vitro and in vivo acetylation of RelA/p65 by...
Sasada, Tetsuro, Ghendler, Yoseph, Neveu, John M., Lane, William S., Reinherz, Ellis L.
Peptide fragments of self-proteins bound to major histocompatibility complex molecules within the thymus are important for positively selecting T cell receptor (TCR)-bearing CD4+CD8+ double positive...
ABRF-PRG04: Differentiation of Protein Isoforms
Arnott, David, Gawinowicz, Mary Ann, Kowalak, Jeffrey A., Lane, William S., Speicher, Kaye D., Turck, Christoph W., ...
Accurate protein identification sometimes requires careful discrimination between closely related protein isoforms that may differ by as little as a single amino acid substitution or...
ABRF-PRG05: De Novo Peptide Sequence Determination
Falick, Arnold M., Kowalak, Jeffrey A., Lane, William S., Phinney, Brett S., Turck, Christoph W., Weintraub, Susan T., ...
A common request of proteomics core facilities is protein identification. However, in some instances primary sequence information for the protein in question is not present in public databases. In...
Epitope tagging of endogenous genes in diverse human cell lines
Kim, Jung-Sik, Bonifant, Challice, Bunz, Fred, Lane, William S., Waldman, Todd
Epitope tagging is a powerful and commonly used approach for studying the physical properties of proteins and their functions and localization in eukaryotic cells. In the case of Saccharomyces...
Phylogenomics of Unusual Histone H2A Variants in Bdelloid Rotifers
Van Doninck, Karine, Mandigo, Morgan L., Hur, Jae H., Wang, Peter, Guglielmini, Julien, Milinkovitch, Michel C., ...
Rotifers of Class Bdelloidea are remarkable in having evolved for millions of years, apparently without males and meiosis. In addition, they are unusually resistant to desiccation and ionizing...
Chavez, Jose A., Roach, William G., Keller, Susanna R., Lane, William S., Lienhard, Gustav E.
Insulin increases glucose transport by stimulating the trafficking of intracellular GLUT4 to the cell surface, a process known as GLUT4 translocation. A key protein in signaling this process is...
Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis
Whyte, Jacqueline, Bader, Jason R., Tauhata, Sinji B.F., Raycroft, Maurice, Hornick, Jessica, Pfister, K. Kevin, ...
Cytoplasmic dynein functions at several sites during mitosis; however, the basis of targeting to each site remains unclear. Tandem mass spectrometry analysis of mitotic dynein revealed a...
Shaw, Bryan F., Schneider, Gregory F., Bilgiçer, Başar, Kaufman, George K., Neveu, John M., Lane, William S., ...
This paper reports that the acetylation of lysine ε-NH3 + groups of α-amylase—one of the most important hydrolytic enzymes used in industry—produces highly negatively charged variants that are...