Maeder, Dennis L., Anderson, Iain, Brettin, Thomas S., Bruce, David C., Gilna, Paul, Han, Cliff S., ...
Metcalf, William W., Zhang, Jun Kai, Apolinario, Ethel, Sowers, Kevin R., Wolfe, Ralph S.
New methods that allow, for the first time, genetic analysis in Archaea of the genus Methanosarcina are presented. First, several autonomously replicating plasmid shuttle vectors have been...
Metcalf, William W., Zhang, Jun Kai, Wolfe, Ralph S.
To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to...
Molecular Genetic Analysis of Phosphite and Hypophosphite Oxidation by Pseudomonas stutzeri WM88
Metcalf, William W., Wolfe, Ralph S.
The first molecular and genetic characterization of a biochemical pathway for oxidation of the reduced phosphorus (P) compounds phosphite and hypophosphite is reported. The pathway was identified in...
Boccazzi, Paolo, Zhang, Jun Kai, Metcalf, William W.
Currently, only one selectable marker is available for genetic studies in the archaeal genus Methanosarcina. Here we report the generation of selectable markers that encode resistance to pseudomonic...
Meuer, Jörn, Kuettner, H. Craig, Zhang, Jun Kai, Hedderich, Reiner, Metcalf, William W.
Ech hydrogenase (Ech) from the methanogenic archaeon Methanosarcina barkeri catalyzes the reversible reduction of ferredoxin by H2 and is a member of a distinct group of membrane-bound [NiFe]...
Zhang, Jun Kai, White, Andrea K., Kuettner, H. Craig, Boccazzi, Paolo, Metcalf, William W.
We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of...
Sluis, Miriam K., Larsen, Rachel A., Krum, Jonathan G., Anderson, Ruth, Metcalf, William W., Ensign, Scott A.
Acetone carboxylase is the key enzyme of bacterial acetone metabolism, catalyzing the condensation of acetone and CO2 to form acetoacetate. In this study, the acetone carboxylase of the purple...
The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity
Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...
Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...
Pritchett, Matthew A., Zhang, Jun Kai, Metcalf, William W.
A new genetic technique for constructing mutants of Methanosarcina acetivorans C2A by using hpt as a counterselectable marker was developed. Mutants with lesions in the hpt gene, encoding...
Yang, Kechao, Metcalf, William W.
Genetic analysis indicates that Escherichia coli possesses two independent pathways for oxidation of phosphite (Pt) to phosphate. One pathway depends on the 14-gene phn operon, which encodes the...
White, Andrea K., Metcalf, William W.
DNA sequencing and analysis of two distinct C—P lyase operons in Pseudomonas stutzeri WM88 were completed. The htxABCDEFGHIJKLMN operon encodes a hypophosphite-2-oxoglutarate dioxygenase (HtxA),...
The htx and ptx Operons of Pseudomonas stutzeri WM88 Are New Members of the Pho Regulon
White, Andrea K., Metcalf, William W.
The htx and ptx operons of Pseudomonas stutzeri WM88 allow for the use of the inorganic reduced phosphorus (P) compounds hypophosphite (P valence, +1) and phosphite (P valence, +3) as sole P sources....
Rother, Michael, Metcalf, William W.
All methanogenic Archaea examined to date rely on methanogenesis as their sole means of energy conservation. Among these are ones that use carbon monoxide as a growth substrate, producing methane via...
Blodgett, Joshua A. V., Zhang, Jun Kai, Metcalf, William W.
A fosmid library from genomic DNA of Streptomyces viridochromogenes DSM 40736 was constructed and screened for the presence of genes known to be involved in the biosynthesis of phosphinothricin...
Wilson, Marlena M., Metcalf, William W.
Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was...
Welander, Paula V., Metcalf, William W.
In the methanogenic archaeon Methanosarcina barkeri Fusaro, the N5-methyl-tetrahydrosarcinapterin (CH3-H4SPT):coenzyme M (CoM) methyltransferase, encoded by the mtr operon, catalyzes the...
Metcalf, William W., Zhang, Jun Kai, Apolinario, Ethel, Sowers, Kevin R., Wolfe, Ralph S.
New methods that allow, for the first time, genetic analysis in Archaea of the genus Methanosarcina are presented. First, several autonomously replicating plasmid shuttle vectors have been...
Metcalf, William W., Zhang, Jun Kai, Wolfe, Ralph S.
To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to...
Molecular Genetic Analysis of Phosphite and Hypophosphite Oxidation by Pseudomonas stutzeri WM88
Metcalf, William W., Wolfe, Ralph S.
The first molecular and genetic characterization of a biochemical pathway for oxidation of the reduced phosphorus (P) compounds phosphite and hypophosphite is reported. The pathway was identified in...
Boccazzi, Paolo, Zhang, Jun Kai, Metcalf, William W.
Currently, only one selectable marker is available for genetic studies in the archaeal genus Methanosarcina. Here we report the generation of selectable markers that encode resistance to pseudomonic...
Meuer, Jörn, Kuettner, H. Craig, Zhang, Jun Kai, Hedderich, Reiner, Metcalf, William W.
Ech hydrogenase (Ech) from the methanogenic archaeon Methanosarcina barkeri catalyzes the reversible reduction of ferredoxin by H2 and is a member of a distinct group of membrane-bound [NiFe]...
Zhang, Jun Kai, White, Andrea K., Kuettner, H. Craig, Boccazzi, Paolo, Metcalf, William W.
We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of...
Sluis, Miriam K., Larsen, Rachel A., Krum, Jonathan G., Anderson, Ruth, Metcalf, William W., Ensign, Scott A.
Acetone carboxylase is the key enzyme of bacterial acetone metabolism, catalyzing the condensation of acetone and CO2 to form acetoacetate. In this study, the acetone carboxylase of the purple...
The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity
Galagan, James E., Nusbaum, Chad, Roy, Alice, Endrizzi, Matthew G., Macdonald, Pendexter, FitzHugh, Will, ...
Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived...
Pritchett, Matthew A., Zhang, Jun Kai, Metcalf, William W.
A new genetic technique for constructing mutants of Methanosarcina acetivorans C2A by using hpt as a counterselectable marker was developed. Mutants with lesions in the hpt gene, encoding...
Yang, Kechao, Metcalf, William W.
Genetic analysis indicates that Escherichia coli possesses two independent pathways for oxidation of phosphite (Pt) to phosphate. One pathway depends on the 14-gene phn operon, which encodes the...
White, Andrea K., Metcalf, William W.
DNA sequencing and analysis of two distinct C—P lyase operons in Pseudomonas stutzeri WM88 were completed. The htxABCDEFGHIJKLMN operon encodes a hypophosphite-2-oxoglutarate dioxygenase (HtxA),...
The htx and ptx Operons of Pseudomonas stutzeri WM88 Are New Members of the Pho Regulon
White, Andrea K., Metcalf, William W.
The htx and ptx operons of Pseudomonas stutzeri WM88 allow for the use of the inorganic reduced phosphorus (P) compounds hypophosphite (P valence, +1) and phosphite (P valence, +3) as sole P sources....
Rother, Michael, Metcalf, William W.
All methanogenic Archaea examined to date rely on methanogenesis as their sole means of energy conservation. Among these are ones that use carbon monoxide as a growth substrate, producing methane via...
Blodgett, Joshua A. V., Zhang, Jun Kai, Metcalf, William W.
A fosmid library from genomic DNA of Streptomyces viridochromogenes DSM 40736 was constructed and screened for the presence of genes known to be involved in the biosynthesis of phosphinothricin...
Wilson, Marlena M., Metcalf, William W.
Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was...
Welander, Paula V., Metcalf, William W.
In the methanogenic archaeon Methanosarcina barkeri Fusaro, the N5-methyl-tetrahydrosarcinapterin (CH3-H4SPT):coenzyme M (CoM) methyltransferase, encoded by the mtr operon, catalyzes the...
Bose, Arpita, Pritchett, Matthew A., Rother, Michael, Metcalf, William W.
Genetic analysis of the three methanol-specific methyltransferase 1 operons (mtaCB1, mtaCB2, and mtaCB3) in Methanosarcina acetivorans led to the suggestion that each of them has a discrete function...
Maeder, Dennis L., Anderson, Iain, Brettin, Thomas S., Bruce, David C., Gilna, Paul, Han, Cliff S., ...
We report here a comparative analysis of the genome sequence of Methanosarcina barkeri with those of Methanosarcina acetivorans and Methanosarcina mazei. The genome of M. barkeri is distinguished by...
Maeder, Dennis L., Anderson, Iain, Brettin, Thomas S., Bruce, David C., Gilna, Paul, Han, Cliff S., ...
Welander, Paula V., Metcalf, William W.
A series of Methanosarcina barkeri mutants lacking the genes encoding the enzymes involved in the C1 oxidation/reduction pathway were constructed. Mutants lacking the methyl-tetrahydromethanopterin...
Bose, Arpita, Pritchett, Matthew A., Metcalf, William W.
The entry of methanol into the methylotrophic pathway of methanogenesis is mediated by the concerted effort of two methyltransferases, namely, methyltransferase 1 (MT1) and methyltransferase 2 (MT2)....