Wim De Clercq

Review on solving the forward problem in EEG source analysis (2007)

Hallez, Hans, Vanrumste, Bart, Grech, Roberta, Muscat, Joseph, De Clercq, Wim, Vergult, Anneleen, ...

Abstract Background The aim of electroencephalogram (EEG) source localization is to find the brain areas responsible for EEG waves of interest. It consists of solving forward and inverse problems....

Caspases leave the beaten track: caspase-mediated activation of NF-kappa B (2006)

Lamkanfi, M, De Clercq, Wim; U0034341, Vanden Berghe, Kristine; U0005211, Vanden Abeele, P

The proteolytic activity of the cysteinyl aspartate-specific proteases, named caspases, mainly connotes their central role in apoptosis and inflammation. In this review we report on recent data on...

Advanced preprocessing techniques and nonlinear signal analysis applied to scalp electroencephalograms for the prediction of epileptic seizures (2005)

De Clercq, Wim

Door het opmeten van potentiaalverschillen tussen elektrodes die geplaatst zijn op de schedel meet het elektro-encefalogram (EEG) de elektrische activiteit van de hersenen. Het EEG is waardevol in de...

Advanced preprocessing techniques and nonlinear signal analysis applied to scalp electroencephalograms for the prediction of epileptic seizures (2005)

De Clercq, Wim

Door het opmeten van potentiaalverschillen tussen elektrodes die geplaatst zijn op de schedel meet het elektro-encefalogram (EEG) de elektrische activiteit van de hersenen. Het EEG is waardevol in de...

Induction of apoptosis by TNF receptor 2 in a T-cell hybridoma is FADD dependent and blocked by caspase-8 inhibitors (2005)

Depuydt, B, Van Loo, G, Vanden Abeele, P, De Clercq, Wim; U0034341

Previously we reported that both human TNFR1 and TNFR2 mediate TNF-induced apoptosis in the transfected rat/mouse T cell hybridoma PC60. We show here that TNFR2-mediated apoptosis in PVC60 cells can...

INCA, a novel human caspase recruitment domain protein that inhibits interleukin-1 beta generation (2004)

Lamkanfi, M, Denecker, G, Kalai, M, D'hondt, K, Meeus, A, De Clercq, Wim; U0034341, ...

Using in silico methods for screening the human genome for new caspase recruitment domain (CARD) proteins, we have identified INCA ( Inhibitory CARD) as a protein that shares 81% identity with the...

Caspase-1 activates nuclear factor of the kappa-enhancer in B cells independently of its enzymatic activity (2004)

Lamkanfi, M, Kalai, M, Saelens, X, De Clercq, Wim; U0034341, Vanden Abeele, P

The proteolytic activity of caspases is involved in apoptosis and inflammation. In this regard, caspase-1 is required for pro-interleukin (IL)-1beta and pro-IL-18 maturation. We report here on a...

Differential signaling to apoptotic and necrotic cell death by Fas-associated death domain protein FADD (2004)

Vanden Berghe, Kristine; U0005211, Van Loo, G, Saelens, X, Van Gurp, M, Brouckaert, G, Kalai, M, ...

Two general pathways for cell death have been defined, apoptosis and necrosis. Previous studies in Jurkat cells have demonstrated that the Fas-associated death domain (FADD) is required for...

Vitamin D3 induces caspase-14 expression in psoriatic lesions and enhances caspase-14 processing in organotypic skin cultures (2004)

Lippens, S, Kockx, M, Denecker, G, Knaapen, M, Verheyen, A, Christiaen, R, ...

Caspase-14 is a nonapoptotic caspase family member whose expression in the epidermis is confined to the suprabasal layers, which consist of differentiating keratinocytes. Proteolytic activation of...

Regulation of the expression and processing of caspase-12 (2003)

Kalai, M, Lamkanfi, M, Denecker, G, Boogmans, M, Lippens, S, Meeus, A, ...

Phylogenetic analysis clusters caspase-12 with the inflammatory caspases 1 and 11. We analyzed the expression of caspase-12 in mouse embryos, adult organs, and different cell types and tested the...

Disruption of HSP90 function reverts tumor necrosis factor-induced necrosis to apoptosis (2003)

Vanden Berghe, Kristine; U0005211, Kalai, M, Van Loo, G, De Clercq, Wim; U0034341, Vanden Abeele, P

Triggering tumor necrosis factor receptor-1 (TNFR1) induces apoptosis in various cell lines. In contrast, stimulation of TNFR1 in L929sA leads to necrosis. Inhibition of HSP90, a chaperone for many...

A matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) analysis of proteins released from isolated liver mitochondria treated with recombinant truncated Bid (2002)

Van Loo, G, Demol, H, Van Gurp, M, Hoorelbeke, B, Schotte, P, Beyaert, R, ...

A crucial event in the process of apoptosis is caspase-dependent generation of truncated Bid (tBid), inducing release of cytochrome c. In an in vitro reconstitution system we combined purified...

The serine protease Omi/HtrA2 is released from mitochondria during apoptosis. Omi interacts with caspase-inhibitor XIAP and induces enhanced caspase activity (2002)

Van Loo, G, Van Gurp, M, Depuydt, B, Srinivasula, SM, Rodriguez, I, Alnemri, ES, ...

Proteome analysis of supernatant of isolated mitochondria exposed to recombinant tBid, a proapoptotic Bcl-2 member, revealed the presence of the serine protease Omi, also called HtrA2. This release...

Endonuclease G: a mitochondrial protein released in apoptosis and involved in caspase-independent DNA degradation (2001)

Van Loo, G, Schotte, P, Van Gurp, M, Demol, H, Hoorelbeke, B, Gevaert, K, ...

A hallmark of apoptosis is the fragmentation of nuclear DNA. Although this activity involves the caspase-3-dependent DNAse CAD (caspase-activated DNAse), evidence exists that DNA fragmentation can...

Heterotrimers formed by tumor necrosis factors of different species or muteins (2001)

Ameloot, P, De Clercq, Wim; U0034341, Fiers, W, Vanden Abeele, P, Brouckaert, P

Incubation of murine tumor necrosis factor (mTNF) at subnanomolar concentrations results in partial dissociation of the trimers, coinciding with a decrease in bioactivity. Using size-exclusion...

Yersinia enterocolitica YopP-induced apoptosis of macrophages involves the apoptotic signaling cascade upstream of Bid (2001)

Denecker, G, De Clercq, Wim; U0034341, Geuijen, CAW, Boland, A, Benabdillah, R, Van Gurp, M, ...

Yersinia enterocolitica induces apoptosis in macrophages by injecting the plasmid-encoded YopP (YopJ in other Yersinia species). Recently it was reported that YopP/J is a member of an ubiquitin-like...

Terminal differentiation of human keratinocytes and stratum corneum formation is associated with caspase-14 activation (2000)

Eckhart, L, De Clercq, Wim; U0034341, Ban, J, Rendl, M, Lengauer, B, Mayer, C, ...

Programmed cell death of epidermal keratinocytes (KC) results in the formation of cornified cells, which constitute the outermost skin layer, the stratum corneum. Here we show by reverse...

Phosphatidyl serine exposure during apoptosis precedes release of cytochrome c and decrease in mitochondrial transmembrane potential (2000)

Denecker, G, Dooms, H, Van Loo, G, Vercammen, D, Grooten, J, Fiers, W, ...

Time kinetics of phosphatidyl serine (PS) exposure were compared to other apoptotic parameters following different apoptotic stimuli, Our data indicate that anti-Fas treatment of L929sAhFas cells...

Proteolytic cleavage of beta-catenin by caspases: an in vitro analysis (1999)

Berx, G, Van Den Brande, Inge; U0005631, Fiers, W, De Clercq, Wim; U0034341, Vanden Abeele, P

Cleavage of structural proteins by caspases has been associated with the severe morphological changes occurring during the apoptotic process. One of the proteins regulating the connection of the...

Identification of caspases that cleave presenilin-1 and presenilin-2 - Five presenilin-1 (PS1) mutations do not alter the sensitivity of PS1 to caspases (1999)

De Jonghe, C, Van Den Brande, Inge; U0005631, De Clercq, Wim; U0034341, Van Gassen, G, Van Criekinge, W, ...

Mutations in the presenilin (PS) genes PS1 and PS2 are involved in Alzheimer's disease (AD), Recently, apoptosis-associated cleavage of PS proteins was identified. Here we demonstrate that PS1 as...

Prospections à Steene-Pitgam: analyse d’un ensemble de terre sigillée (1999)

De Clercq, Wim, Deschieter, Johan

Over the past few years, the amateur archaeologist Ph. Ducrocq systematically prospected the surroundings of the communes of Steene-Pitgam. The eleven "dumping grounds" of archaeological artefacts...