Caruthers, Jonathan, Zucker, Frank, Worthey, Elizabeth, Myler, Peter J., Buckner, Fred, Van Voorhuis, Wes, ...
We have determined the crystal structures of three homologous proteins from the pathogenic protozoans Leishmania donovani, Leishmania major, and Trypanosoma cruzi. We propose that these proteins...
Quigley, Paulene M., Korotkov, Konstantin, Baneyx, François, Hol, Wim G.J.
Heat shock proteins and proteases play a crucial role in cell survival under conditions of environmental stress. The heat shock protein Hsp31, produced by gene hchA at elevated temperatures in...
An improved crystal form of Plasmodium falciparum peptide deformylase (2004)
Robien, Mark A., Nguyen, Kiet T., Kumar, Abhinav, Hirsh, Irwin, Turley, Stewart, Pei, Dehua, ...
An altered version of peptide deformylase from Plasmodium falciparum (PfPDF), the organism that causes the most devastating form of malaria, has been cocrystallized with a synthesized inhibitor that...
An improved crystal form of Plasmodium falciparum peptide deformylase (2004)
Robien, Mark A., Nguyen, Kiet T., Kumar, Abhinav, Hirsh, Irwin, Turley, Stewart, Pei, Dehua, ...
An altered version of peptide deformylase from Plasmodium falciparum (PfPDF), the organism that causes the most devastating form of malaria, has been cocrystallized with a synthesized inhibitor that...
Protein structure-based design of anti-protozoal drugs (2002)
Bressi,Jerome C., Choe,Jungwoo, Suresh,Stephen, Buckner,Frederick S., Van Voorhis,Wesley C., ...
The repertory of drugs to fight protozoal diseases such as malaria, Chagas' disease, leishmaniasis, and African trypanosomiasis is woefully inadequate. Now, genome sequencing and structural genomics...
Dijkema, Rein, Mulders, John W.M., Grootenhuis, Peter D.J., Hol, Wim G.J., Kalk, Kor H., Boer, Bijtske De, ...
The three-dimensional structure of antistasin, a potent inhibitor of blood coagulation factor Xa, from the Mexican leech Haementeria officinalis was determined at 1.9 Å resolution by X-ray...
Schreuder, Herman A., Krengel, Ute, Lapatto, Risto, Arkema, Anita, Boer, Bijtske De, Kalk, Kor H., ...
The three-dimensional structure of antistasin, a potent inhibitor of blood coagulation factor Xa, from the Mexican leech Haementeria officinalis was determined at 1.9 Å resolution by X-ray...
Three hTIM Mutants that Provide New Insights on why TIM is a Dimer (1996)
Mainfroid, Véronique, Terpstra, Peter, Beauregard, Marc, Frère, Jean-Marie, Mande, Shekhar C., Hol, Wim G.J., ...
Human triosephosphate isomerase (hTIM), a dimeric enzyme, was altered by site-directed mutagenesis in order to determine whether it can be dissociated into monomers. Two hTIM mutants were produced,...
Mainfroid, Véronique, Mande, Shekhar C, Hol, Wim G J, Martial, Joseph, Goraj, Karine
Human triosephosphate isomerase (hTIM) is a dimeric enzyme of identical subunits, adopting the alpha/beta-barrel fold. In a previous work, a monomeric mutant of hTIM was engineered in which Met14 and...
Rentier-Delrue, Françoise, Mande, Shekhar C., Moyens, Sylvianne, Terpstra, Peter, Mainfroid, Véronique, Goraj, Karine, ...
We focused on the temperature adaptation of triosephosphate isomerase (TIM; E.C. 5.3.1.1.) by comparing the structure of TIMs isolated from bacterial organisms living in either cold or hot...
Sixma, Titia K., Aguirre, Angel, Terwisscha Van Scheltinga, Anke C., Wartna, Ellen S., Kalk, Kor H., Hol, Wim G.J.
A new native crystal form of heat-labile enterotoxin (LT) has two AB5 complexes in the asymmetric unit with different orientations of the A subunit with respect to the B pentamer. Comparison with...
Wartna, Ellen S., Zhou, Kangjing, Kalk, Kor H., Terwisscha Van Scheltinga, Anke C., Sixma, Titia K., Hol, Wim G.J.
The crystal structure determination of heat labile enterotoxin (LT) bound to two different lanthanide ions, erbium and samarium, revealed two distinct ion binding sites in the interface of the A...
Opperdoes, Fred R., Lambeir, Anne-Marie, Wierenga, Rik K., Noble, Martin E.M., Kalk, Kor H., ...
Crystals of triosephosphate isomerase from Trypanosoma brucei brucei have been used in binding studies with three competitive inhibitors of the enzyme's activity. Highly refined structures have been...
Kalk, Kor H., Groendijk, Hillie, Opperdoes, Fred R., Lambeir, Anne-Marie, Hol, Wim G.J., ...
Crystals of triosephosphate isomerase from Trypanosoma brucei brucei have been used in binding studies with three competitive inhibitors of the enzyme's activity. Highly refined structures have been...
Kalk, Kor H., Dijkstra, Bauke W., Fujinaga, Masao, Gros, Piet, Hol, Wim G.J.
In order to investigate the principles of protein thermostability, the crystal structure of thermitase from Thermoactinomyces vulgaris, a thermostable member of the subtilisin family of serine...
Harder, Wim, Veenhuis, Marten, Dijkstra, Bauke W., Rozeboom, Henriëtte, Lawson, Catherine L., ...
Alcohol oxidase, purified from the yeast Hansenula polymorpha, was crystallized in vitro for the purpose of determining its structure at atomic resolution by X-ray diffraction methods. The crystals...
Hol, Wim G.J., Kalk, Kor H., Dijkstra, Bauke W., Fujinaga, Masao, Gros, Piet
In order to investigate the principles of protein thermostability, the crystal structure of thermitase from Thermoactinomyces vulgaris, a thermostable member of the subtilisin family of serine...
Rozeboom, Henriëtte, Lawson, Catherine L., Dijkstra, Bauke W., Veenhuis, Marten, Harder, Wim, ...
Alcohol oxidase, purified from the yeast Hansenula polymorpha, was crystallized in vitro for the purpose of determining its structure at atomic resolution by X-ray diffraction methods. The crystals...
Automated protein crystallization and a new crystal form of a subtilisin:eglin complex (1987)
Kelders, Henk A., Kalk, Kor H., Gros, Piet, Hol, Wim G.J.
A procedure is described for automating labour-intensive steps of the ‘hanging drop’ protein crystallization method. An automatic sample changer is employed to fill the wells in a multi-well...
Wierenga, Rik K., Terpstra, Peter, Hol, Wim G.J.
An amino acid sequence "fingerprint” has been derived that can be used to test if a particular sequence will fold into a βαβ-unit with ADP-binding properties. It was deduced from a careful...
Hol, Wim G.J., Kalk, Kor H., Renetseder, Roland, Dijkstra, Bauke W., Drenth, Jan
The previously published three-dimensional structure of porcine pancreatic prophospholipase A2 at 3 Å resolution was found to be incompatible with the structures of bovine phospholipase A2 and...
Hol, Wim G.J., Kalk, Kor H., Renetseder, Roland, Dijkstra, Bauke W., Drenth, Jan
The previously published three-dimensional structure of porcine pancreatic prophospholipase A2 at 3 Å resolution was found to be incompatible with the structures of bovine phospholipase A2 and...
The Structure of Bovine Pancreatic Prophospholipase A2 at 3.0 Å Resolution (1982)
Hol, Wim G.J., Brandenburg, Niko P., Kalk, Kor H., Dijkstra, Bauke W., Drenth, Jan
Bovine pancreatic prophospholipase A2, the precursor of the lipolytic enzyme phospholipase A2, crystallizes in space group P3121 with cell dimensions a = b = 46.95, c = 102.0 Å. The structure was...
The Structure of Bovine Pancreatic Prophospholipase A2 at 3.0 Å Resolution (1982)
Kalk, Kor H., Dijkstra, Bauke W., Brandenburg, Niko P., Hol, Wim G.J., Drenth, Jan
Bovine pancreatic prophospholipase A2, the precursor of the lipolytic enzyme phospholipase A2, crystallizes in space group P3121 with cell dimensions a = b = 46.95, c = 102.0 Å. The structure was...
Structure of Bovine Pancreatic Phospholipase A2 at 1.7Å Resolution (1981)
Hol, Wim G.J., Kalk, Kor H., Dijkstra, Bauke W., Drenth, Jan
The crystal structure of bovine pancreatic phospholipase A2 has been refined to 1.7 Å resolution. The starting model for this refinement was the previously published structure at a resolution of 2.4...
Structure of Bovine Pancreatic Phospholipase A2 at 1.7Å Resolution (1981)
Hol, Wim G.J., Kalk, Kor H., Dijkstra, Bauke W., Drenth, Jan
The crystal structure of bovine pancreatic phospholipase A2 has been refined to 1.7 Å resolution. The starting model for this refinement was the previously published structure at a resolution of 2.4...
Izard, Tina, Ævarsson, Arnthor, Allen, Mark D., Westphal, Adrie H., Perham, Richard N., De Kok, Aart, ...
The pyruvate dehydrogenase multienzyme complex (Mr of 5–10 million) is assembled around a structural core formed of multiple copies of dihydrolipoyl acetyltransferase (E2p), which exhibits the...
Aronov, Alex M., Suresh, Stephen, Buckner, Frederick S., Van Voorhis, Wesley C., Opperdoes, Fred R., ...
The bloodstream stage of Trypanosoma brucei and probably the intracellular (amastigote) stage of Trypanosoma cruzi derive all of their energy from glycolysis. Inhibiting glycolytic enzymes may be a...
Rudenko, Gabby, Bonten, Erik, Hol, Wim. G. J., D’Azzo, Alessandra
Human protective protein/cathepsin A (PPCA), a serine carboxypeptidase, forms a multienzyme complex with β-galactosidase and neuraminidase and is required for the intralysosomal activity and...
Goranson-Siekierke, Joanne, Pohl, Ehmke, Hol, Wim G. J., Holmes, Randall K.
The homodimeric diphtheria toxin repressor (DtxR) uses Fe2+ as a corepressor, binds to iron-regulated promoters, and negatively regulates the syntheses of diphtheria toxin, corynebacterial...
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding
Martinez, Sergio E., Wu, Albert Y., Glavas, Natalie A., Tang, Xiao-Bo, Turley, Stewart, Hol, Wim G. J., ...
Cyclic nucleotide phosphodiesterases (PDEs) regulate all pathways that use cGMP or cAMP as a second messenger. Five of the 11 PDE families have regulatory segments containing GAF domains, 3 of which...
Quigley, Paulene M., Korotkov, Konstantin, Baneyx, François, Hol, Wim G. J.
Heat shock proteins (Hsps) play essential protective roles under stress conditions by preventing the formation of protein aggregates and degrading misfolded proteins. EcHsp31, the yedU (hchA) gene...
Tinker, Juliette K., Erbe, Jarrod L., Hol, Wim G. J., Holmes, Randall K.
Cholera toxin (CT) and related Escherichia coli enterotoxins LTI and LTIIb have a conserved hydrophobic region at the AB5 interface postulated to be important for toxin assembly. Hydrophobic residue...
Sites of Interaction between Aldolase and Thrombospondin-related Anonymous Protein in Plasmodium
Buscaglia, Carlos A., Coppens, Isabelle, Hol, Wim G. J., Nussenzweig, Victor
Gliding motility and host cell invasion by apicomplexan parasites are empowered by an acto-myosin motor located underneath the parasite plasma membrane. The motor is connected to host cell receptors...
Sastry, M. S. R., Quigley, Paulene M., Hol, Wim G. J., Baneyx, François
Precise control of substrate binding and release is essential for molecular chaperones to exert their protective function in times of stress. The mechanisms used are diverse and have been difficult...
Two Sensor Kinases Contribute to the Hypoxic Response of Mycobacterium tuberculosis*
Roberts, David M., Liao, Reiling P., Wisedchaisri, Goragot, Hol, Wim G. J., Sherman, David R.
Current estimates indicate that nearly a third of the world's population is latently infected with Mycobacterium tuberculosis. Reduced oxygen tension and nitric oxide exposure are two conditions...
Structure of the MTIP–MyoA complex, a key component of the malaria parasite invasion motor
Bosch, Jürgen, Turley, Stewart, Daly, Thomas M., Bogh, Stephen M., Villasmil, Michelle L., Roach, Claudia, ...
The causative agents of malaria have developed a sophisticated machinery for entering multiple cell types in the human and insect hosts. In this machinery, a critical interaction occurs between the...
Izard, Tina, Ævarsson, Arnthor, Allen, Mark D., Westphal, Adrie H., Perham, Richard N., De Kok, Aart, ...
The pyruvate dehydrogenase multienzyme complex (Mr of 5–10 million) is assembled around a structural core formed of multiple copies of dihydrolipoyl acetyltransferase (E2p), which exhibits the...
Aronov, Alex M., Suresh, Stephen, Buckner, Frederick S., Van Voorhis, Wesley C., Opperdoes, Fred R., ...
The bloodstream stage of Trypanosoma brucei and probably the intracellular (amastigote) stage of Trypanosoma cruzi derive all of their energy from glycolysis. Inhibiting glycolytic enzymes may be a...
Rudenko, Gabby, Bonten, Erik, Hol, Wim. G. J., D’Azzo, Alessandra
Human protective protein/cathepsin A (PPCA), a serine carboxypeptidase, forms a multienzyme complex with β-galactosidase and neuraminidase and is required for the intralysosomal activity and...
Goranson-Siekierke, Joanne, Pohl, Ehmke, Hol, Wim G. J., Holmes, Randall K.
The homodimeric diphtheria toxin repressor (DtxR) uses Fe2+ as a corepressor, binds to iron-regulated promoters, and negatively regulates the syntheses of diphtheria toxin, corynebacterial...
The two GAF domains in phosphodiesterase 2A have distinct roles in dimerization and in cGMP binding
Martinez, Sergio E., Wu, Albert Y., Glavas, Natalie A., Tang, Xiao-Bo, Turley, Stewart, Hol, Wim G. J., ...
Cyclic nucleotide phosphodiesterases (PDEs) regulate all pathways that use cGMP or cAMP as a second messenger. Five of the 11 PDE families have regulatory segments containing GAF domains, 3 of which...
Quigley, Paulene M., Korotkov, Konstantin, Baneyx, François, Hol, Wim G. J.
Heat shock proteins (Hsps) play essential protective roles under stress conditions by preventing the formation of protein aggregates and degrading misfolded proteins. EcHsp31, the yedU (hchA) gene...
Tinker, Juliette K., Erbe, Jarrod L., Hol, Wim G. J., Holmes, Randall K.
Cholera toxin (CT) and related Escherichia coli enterotoxins LTI and LTIIb have a conserved hydrophobic region at the AB5 interface postulated to be important for toxin assembly. Hydrophobic residue...
Sites of Interaction between Aldolase and Thrombospondin-related Anonymous Protein in Plasmodium
Buscaglia, Carlos A., Coppens, Isabelle, Hol, Wim G. J., Nussenzweig, Victor
Gliding motility and host cell invasion by apicomplexan parasites are empowered by an acto-myosin motor located underneath the parasite plasma membrane. The motor is connected to host cell receptors...
Sastry, M. S. R., Quigley, Paulene M., Hol, Wim G. J., Baneyx, François
Precise control of substrate binding and release is essential for molecular chaperones to exert their protective function in times of stress. The mechanisms used are diverse and have been difficult...
Structure of the MTIP–MyoA complex, a key component of the malaria parasite invasion motor
Bosch, Jürgen, Turley, Stewart, Daly, Thomas M., Bogh, Stephen M., Villasmil, Michelle L., Roach, Claudia, ...
The causative agents of malaria have developed a sophisticated machinery for entering multiple cell types in the human and insect hosts. In this machinery, a critical interaction occurs between the...
Bosch, Jürgen, Buscaglia, Carlos A., Krumm, Brian, Ingason, Bjarni P., Lucas, Robert, Roach, Claudia, ...
An actomyosin motor located underneath the plasma membrane drives motility and host-cell invasion of apicomplexan parasites such as Plasmodium falciparum and Plasmodium vivax, the causative agents of...
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids
Camberg, Jodi L, Johnson, Tanya L, Patrick, Marcella, Abendroth, Jan, Hol, Wim G J, Sandkvist, Maria
EpsE is a cytoplasmic component of the type II secretion system in Vibrio cholerae. Through ATP hydrolysis and an interaction with the cytoplasmic membrane protein EpsL, EpsE supports secretion of...
Structure of the conserved hypothetical protein MAL13P1.257 from Plasmodium falciparum
Holmes, Margaret A., Buckner, Frederick S., Van Voorhis, Wesley C., Mehlin, Christopher, Boni, Erica, Earnest, Thomas N., ...
The crystal structure of a conserved hypothetical protein, MAL13P1.257 from P. falciparum, has been determined at 2.17 Å resolution. The structure represents a new protein fold and is the first...
Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major
Arakaki, Tracy, Le Trong, Isolde, Phizicky, Eric, Quartley, Erin, DeTitta, George, Luft, Joseph, ...
The crystal structure of a conserved hypothetical protein from L. major, Pfam sequence family PF04543, structural genomics target ID Lmaj006129AAA, has been determined at a resolution of 1.6 Å.
Caruthers, Jonathan, Zucker, Frank, Worthey, Elizabeth, Myler, Peter J., Buckner, Fred, Van Voorhuis, Wes, ...
We have determined the crystal structures of three homologous proteins from the pathogenic protozoans Leishmania donovani, Leishmania major, and Trypanosoma cruzi. We propose that these proteins...
An improved crystal form of Plasmodium falciparum peptide deformylase
Robien, Mark A., Nguyen, Kiet T., Kumar, Abhinav, Hirsh, Irwin, Turley, Stewart, Pei, Dehua, ...
An altered version of peptide deformylase from Plasmodium falciparum (PfPDF), the organism that causes the most devastating form of malaria, has been cocrystallized with a synthesized inhibitor that...
A new native EcHsp31 structure suggests a key role of structural flexibility for chaperone function
Quigley, Paulene M., Korotkov, Konstantin, Baneyx, François, Hol, Wim G.J.
Heat shock proteins and proteases play a crucial role in cell survival under conditions of environmental stress. The heat shock protein Hsp31, produced by gene hchA at elevated temperatures in...
Structure of ribose 5-phosphate isomerase from Plasmodium falciparum
Holmes, Margaret A., Buckner, Frederick S., Van Voorhis, Wesley C., Mehlin, Christopher, Boni, Erica, ...
The crystal structure of ribose 5-phosphate isomerase from P. falciparum has been determined at 2.09 Å resolution. The structure is very similar to those of RpiA from other species, but is in some...
Arakaki, Tracy, Neely, Helen, Boni, Erica, Mueller, Natasha, Buckner, Frederick S., Van Voorhis, Wesley C., ...
The crystal structure of a phosphatidylethanolamine-binding protein from P. vivax, a homolog of Raf-kinase inhibitor protein (RKIP), has been solved to a resolution of 1.3 Å. The inferred...
Structure of a Trypanosoma brucei α/β-hydrolase fold protein with unknown function
Merritt, Ethan A., Holmes, Margaret, Buckner, Frederick S., Van Voorhis, Wesley C., Quartly, Erin, Phizicky, Eric M., ...
T. brucei gene Tb10.6k15.0140 codes for an α/β-hydrolase fold protein of unknown function. The 2.2 Å crystal structure shows that members of this sequence family retain a conserved Ser residue...