Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, (2008)
Helga Westers, Ronald Dorenbos, Jorrit Kabel, Tony Flanagan, Kevin M. Devine, ...
Reconstructing the Bacillus genome (Westers et al.) Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is...
Boersma, Ykelien L., Dröge, Melloney J., Pijning, Tjaard, Cool, Robbert H., Dijkstra, Bauke W., ...
In directed evolution experiments, success often depends on the efficacy of screening or selection methods. Genetic selections have proven to be extremely valuable for evolving enzymes with improved...
Loop Grafting of Bacillus subtilis Lipase A: Inversion of Enantioselectivity (2008)
Boersma, Ykelien L., Pijning, Tjaard, Bosma, Margriet S., Godinho, Luís F., Dröge, Melloney J., ...
Lipases are successfully applied in enantioselective biocatalysis. Most lipases contain a lid domain controlling access to the active site, but Bacillus subtilis Lipase A (LipA) is a notable...
Reetz, Manfred T., Dijkstra, Bauke W., Vrenken, Titia E., Rüggeberg, Carsten J., Pouderoyen, Gertie Van, Boersma, Ykelien L., ...
Phage display can be used as a protein-engineering tool for the selection of proteins with desirable binding properties from a library of mutants. Here we describe the application of this method for...
Darmon, Elise, Dorenbos, Ronald, Meens, Jochen, Freudl, Roland, Antelmann, Haike, Hecker, Michael, ...
The gram-positive bacterium Bacillus subtilis secretes high levels of proteins into its environment. Most of these secretory proteins are exported from the cytoplasm in an unfolded state and have to...
Boersma, Ykelien L., Dröge, Melloney J., Pouderoyen, Gertie Van, Vrenken, Titia E., Rüggeberg, Carsten J., Reetz, Manfred T., ...
Phage display can be used as a protein-engineering tool for the selection of proteins with desirable binding properties from a library of mutants. Here we describe the application of this method for...
Plaza Menacho, Ivan, Koster, Roelof, Quax, Wim J., Osinga, Jan, Sluis, Tineke Van Der, ...
The RET proto-oncogene encodes a receptor tyrosine kinase whose dysfunction plays a crucial role in the development of several neural crest disorders. Distinct activating RET mutations cause multiple...
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Stabilization of TRAIL, an all-{beta}-sheet multimeric protein, using computational redesign (2004)
Van Der Sloot, Almer M., Mullally, Margaret M., Fernandez-Ballester, Gregorio, Serrano, Luis, Quax, Wim J.
Protein thermal stability is important for therapeutic proteins, both influencing the pharmacokinetic and pharmacodynamic properties and for stability during production and shelf-life of the final...
Stabilization of TRAIL, an all {beta}-sheet multimeric protein, using computational redesign (2004)
Van Der Sloot, Almer M., Mullally, Margaret M., Fernandez-Ballester, Gregorio, Serrano, Luis, Quax, Wim J.
Protein thermal stability is important for therapeutic proteins, both influencing the pharmacokinetic and pharmacodynamic properties and for stability during production and shelf-life of the final...
Stabilization of TRAIL, an all {beta}-sheet multimeric protein, using computational redesign (2004)
Van Der Sloot, Almer M., Mullally, Margaret M., Fernandez-Ballester, Gregorio, Serrano, Luis, Quax, Wim J.
Protein thermal stability is important for therapeutic proteins, both influencing the pharmacokinetic and pharmacodynamic properties and for stability during production and shelf-life of the final...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Dijl, Jan Maarten Van, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Swaving, Jelto, Broekhuizen, Cees P., Rose, Matthias, Quax, Wim J., Driessen, Arnold J.M.
Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral...
Tjalsma, Harold, Bolhuis, Albert, Van Roosmalen, Maarten L., Wiegert, Thomas, Schumann, Wolfgang, Broekhuizen, Cees P., ...
The genes coding for the cytoskeletal proteins actin and vimentin in warm-blooded vertebrates (1982)
Dodemont, Huub J., Soriano, Philippe, Quax, Wim J., Ramaekers, Frans, Lenstra, Johannes A.
Genes coding for vimentin and actin in mammals and birds (1982)
Quax, Wim J., Dodement, Huub J., Lenstra, Johannes A., Ramaekers, Frans C.S., Soriano, Philippe
Functional Identification of the Product of the Bacillus subtilis yvaL Gene as a SecG Homologue
Swaving, Jelto, Broekhuizen, Cees P., Rose, Matthias, Quax, Wim J., Driessen, Arnold J. M.
Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral...
Development of a Lipase Fermentation Process That Uses a Recombinant Pseudomonas alcaligenes Strain
Gerritse, Gijs, Hommes, Ronald W. J., Quax, Wim J.
Pseudomonas alcaligenes M-1 secretes an alkaline lipase, which has excellent characteristics for the removal of fatty stains under modern washing conditions. A fed-batch fermentation process based on...
Tjalsma, Harold, Bolhuis, Albert, Van Roosmalen, Maarten L., Wiegert, Thomas, Schumann, Wolfgang, Broekhuizen, Cees P., ...
Approximately 47% of the genes of the Gram-positive bacterium Bacillus subtilis belong to paralogous gene families. The present studies were aimed at the functional analysis of the sip gene family of...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Quorum Quenching by an N-Acyl-Homoserine Lactone Acylase from Pseudomonas aeruginosa PAO1
Sio, Charles F., Otten, Linda G., Cool, Robbert H., Diggle, Stephen P., Braun, Peter G., Bos, Rein, ...
The virulence of the opportunistic human pathogen Pseudomonas aeruginosa PAO1 is controlled by an N-acyl-homoserine lactone (AHL)-dependent quorum-sensing system. During functional analysis of...
Dröge, Melloney J., Boersma, Ykelien L., Braun, Peter G., Buining, Robbert Jan, Julsing, Mattijs K., Selles, Karin G. A., ...
Using the phage display technology, a protein can be displayed at the surface of bacteriophages as a fusion to one of the phage coat proteins. Here we describe development of this method for fusion...
Van Der Sloot, Almer M., Tur, Vicente, Szegezdi, Eva, Mullally, Margaret M., Cool, Robbert H., Samali, Afshin, ...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potential anticancer drug that selectively induces apoptosis in a variety of cancer cells by interacting with death receptors DR4...
Functional Identification of the Product of the Bacillus subtilis yvaL Gene as a SecG Homologue
Swaving, Jelto, Broekhuizen, Cees P., Rose, Matthias, Quax, Wim J., Driessen, Arnold J. M.
Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral...
Development of a Lipase Fermentation Process That Uses a Recombinant Pseudomonas alcaligenes Strain
Gerritse, Gijs, Hommes, Ronald W. J., Quax, Wim J.
Pseudomonas alcaligenes M-1 secretes an alkaline lipase, which has excellent characteristics for the removal of fatty stains under modern washing conditions. A fed-batch fermentation process based on...
Tjalsma, Harold, Bolhuis, Albert, Van Roosmalen, Maarten L., Wiegert, Thomas, Schumann, Wolfgang, Broekhuizen, Cees P., ...
Approximately 47% of the genes of the Gram-positive bacterium Bacillus subtilis belong to paralogous gene families. The present studies were aimed at the functional analysis of the sip gene family of...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Quorum Quenching by an N-Acyl-Homoserine Lactone Acylase from Pseudomonas aeruginosa PAO1
Sio, Charles F., Otten, Linda G., Cool, Robbert H., Diggle, Stephen P., Braun, Peter G., Bos, Rein, ...
The virulence of the opportunistic human pathogen Pseudomonas aeruginosa PAO1 is controlled by an N-acyl-homoserine lactone (AHL)-dependent quorum-sensing system. During functional analysis of...
Van Der Sloot, Almer M., Tur, Vicente, Szegezdi, Eva, Mullally, Margaret M., Cool, Robbert H., Samali, Afshin, ...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potential anticancer drug that selectively induces apoptosis in a variety of cancer cells by interacting with death receptors DR4...
Dröge, Melloney J., Boersma, Ykelien L., Braun, Peter G., Buining, Robbert Jan, Julsing, Mattijs K., Selles, Karin G. A., ...
Using the phage display technology, a protein can be displayed at the surface of bacteriophages as a fusion to one of the phage coat proteins. Here we describe development of this method for fusion...
Darmon, Elise , Dorenbos, Ronald, Meens, Jochen, Freudl, Roland, Antelmann, Haike, Hecker, Michael, ...
The gram-positive bacterium Bacillus subtilis secretes high levels of proteins into its environment. Most of these secretory proteins are exported from the cytoplasm in an unfolded state and have to...
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis
Julsing, Mattijs K., Rijpkema, Michael, Woerdenbag, Herman J., Quax, Wim J., Kayser, Oliver
In comparison to other bacteria Bacillus subtilis emits the volatile compound isoprene in high concentrations. Isoprene is the smallest representative of the natural product group of terpenoids. A...
Tarrus, Marc, Van Der Sloot, Almer M., Temming, Kai, Lacombe, Marie, Opdam, Frank, Quax, Wim J., ...
Recombinant TNF-related apoptosis-inducing ligand (TRAIL) is considered a powerful and selective inducer of tumor cell death. We hypothesize that TRAIL’s potential as anticancer agent can be...
Krzeslak, Joanna, Gerritse, Gijs, Van Merkerk, Ronald, Cool, Robbert H., Quax, Wim J.
Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as...
Dubois, Jean-Yves F., Freudl, Roland, Quax, Wim J., Van Dijl, Jan Maarten
Disulfide bonds are important for the correct folding, structural integrity, and activity of many biotechnologically relevant proteins. For synthesis and subsequent secretion of these proteins in...