Mechanism of Ad5 Vaccine Immunity and Toxicity: Fiber Shaft Targeting of Dendritic Cells (2007)
Cheng Cheng, Wing-pui Kong, Rebecca L. Sheets, Phillip L. Gomez, C. Richter King, ...
Recombinant adenoviral (rAd) vectors elicit potent cellular and humoral immune responses and show promise as vaccines for HIV-1, Ebola virus, tuberculosis, malaria, and other infections. These...
Sheets, Rebecca L., Stein, Judith, Manetz, T. Scott, Duffy, Chris, Nason, Martha, Andrews, Charla, ...
The Vaccine Research Center has developed a number of vaccine candidates for different diseases/infectious agents (HIV-1, Severe Acute Respiratory Syndrome virus, West Nile virus, and Ebola virus,...
Sheets, Rebecca L., Stein, Judith, Manetz, T. Scott, Duffy, Chris, Nason, Martha, Andrews, Charla, ...
The Vaccine Research Center has developed a number of vaccine candidates for different diseases/infectious agents (HIV-1, SARS virus, WNV, and Ebola virus, plus a plasmid cytokine adjuvant - IL-2/Ig)...
Friborg, Jacques, Kong, Wing-Pui, Flowers, C. Clay, Flowers, Scarlett L., Sun, Yongnian, Foreman, Kimberly E., ...
The DNA sequence for Kaposi’s sarcoma-associated herpesvirus was originally detected in Kaposi’s sarcoma biopsy specimens. Since its discovery, it has been possible to detect virus in cell lines...
Huang, Yue, Kong, Wing-pui, Nabel, Gary J.
Immunity to human immunodeficiency virus virion-like structures or a polyprotein has been examined after DNA immunization with Rev-independent expression vectors. A Gag-Pol fusion protein stimulated...
Moore, Anne C., Kong, Wing-pui, Chakrabarti, Bimal K., Nabel, Gary J.
The effects of genetic adjuvants on humoral and cell-mediated immunity to two human immunodeficiency virus antigens, Env and Nef, have been examined in mice. Despite similar levels of gene expression...
Baig, Jamal, Levy, Daniel B., McKay, Paul F., Schmitz, Joern E., Santra, Sampa, Subbramanian, Ramu A., ...
Since most human immunodeficiency virus (HIV) infections are initiated following mucosal exposure to the virus, the anatomic containment or abortion of an HIV infection is likely to require...
Chakrabarti, Bimal K., Kong, Wing-pui, Wu, Bei-yue, Yang, Zhi-Yong, Friborg, Jacques, Ling, Xu, ...
In this study, we have investigated the effect of specific mutations in human immunodeficiency virus type 1 (HIV-1) envelope (Env) on antibody production in an effort to improve humoral immune...
Overcoming Immunity to a Viral Vaccine by DNA Priming before Vector Boosting
Yang, Zhi-yong, Wyatt, Linda S., Kong, Wing-pui, Moodie, Zoe, Moss, Bernard, Nabel, Gary J.
Replication-defective adenovirus (ADV) and poxvirus vectors have shown potential as vaccines for pathogens such as Ebola or human immunodeficiency virus in nonhuman primates, but prior immunity to...
Lemiale, Franck, Kong, Wing-pui, Akyürek, Levent M., Ling, Xu, Huang, Yue, Chakrabarti, Bimal K., ...
Replication-defective adenovirus (ADV) vectors represent a promising potential platform for the development of a vaccine for AIDS. Although this vector is typically administered intramuscularly, it...
Immunogenicity of Multiple Gene and Clade Human Immunodeficiency Virus Type 1 DNA Vaccines
Kong, Wing-Pui, Huang, Yue, Yang, Zhi-Yong, Chakrabarti, Bimal K., Moodie, Zoe, Nabel, Gary J.
The ability to elicit an immune response to a spectrum of human immunodeficiency virus type 1 (HIV-1) gene products from divergent strains is a desirable feature of an AIDS vaccine. In this study, we...
Yang, Zhi-yong, Chakrabarti, Bimal K., Xu, Ling, Welcher, Brent, Kong, Wing-pui, Leung, Kwanyee, ...
Although the B clade of human immunodeficiency virus type 1 (HIV-1) envelopes (Env) includes five highly variable regions, each of these domains contains a subset of sequences that remain conserved....
Yang, Zhi-Yong, Huang, Yue, Ganesh, Lakshmanan, Leung, Kwanyee, Kong, Wing-Pui, Schwartz, Owen, ...
The severe acute respiratory syndrome coronavirus (SARS-CoV) synthesizes several putative viral envelope proteins, including the spike (S), membrane (M), and small envelope (E) glycoproteins....
Heterologous Envelope Immunogens Contribute to AIDS Vaccine Protection in Rhesus Monkeys
Letvin, Norman L., Huang, Yue, Chakrabarti, Bimal K., Xu, Ling, Seaman, Michael S., Beaudry, Kristin, ...
Because a strategy to elicit broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies has not yet been found, the role of an Env immunogen in HIV-1 vaccine candidates remains...
Huang, Yue, Yang, Zhi-yong, Kong, Wing-pui, Nabel, Gary J.
The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of...
Mascola, John R., Sambor, Anna, Beaudry, Kristin, Santra, Sampa, Welcher, Brent, Louder, Mark K., ...
Immunization with recombinant serotype 5 adenoviral (rAd5) vectors or a combination of DNA plasmid priming and rAd5 boosting is known to elicit potent immune responses. However, little data exist...
Ebola Virus Glycoprotein Toxicity Is Mediated by a Dynamin-Dependent Protein-Trafficking Pathway
Sullivan, Nancy J., Peterson, Mary, Yang, Zhi-yong, Kong, Wing-pui, Duckers, Heinricus, Nabel, Elizabeth, ...
Ebola virus infection causes a highly lethal hemorrhagic fever syndrome associated with profound immunosuppression through its ability to induce widespread inflammation and cellular damage. Though...
Evasion of antibody neutralization in emerging severe acute respiratory syndrome coronaviruses
Yang, Zhi-yong, Werner, Heidi C., Kong, Wing-pui, Leung, Kwanyee, Traggiai, Elisabetta, Lanzavecchia, Antonio, ...
Molecular characterization of the severe acute respiratory syndrome coronavirus has revealed genetic diversity among isolates. The spike (S) glycoprotein, the major target for vaccine and immune...
Wu, Lan, Kong, Wing-pui, Nabel, Gary J.
A variety of gene-based vaccination approaches have been used to enhance the immune response to viral pathogens. Among them, the ability to perform heterologous immunization by priming with DNA and...
Kong, Wing-pui, Xu, Ling, Stadler, Konrad, Ulmer, Jeffrey B., Abrignani, Sergio, Rappuoli, Rino, ...
Although the initial isolates of the severe acute respiratory syndrome (SARS) coronavirus (CoV) are sensitive to neutralization by antibodies through their spike (S) glycoprotein, variants of S have...
Barouch, Dan H., Yang, Zhi-yong, Kong, Wing-pui, Korioth-Schmitz, Birgit, Sumida, Shawn M., Truitt, Diana M., ...
Plasmid DNA vaccines elicit potent and protective immune responses in numerous small-animal models of infectious diseases. However, their immunogenicity in primates appears less potent. Here we...
Yang, Zhi-yong, Chakrabarti, Bimal K., Xu, Ling, Welcher, Brent, Kong, Wing-pui, Leung, Kwanyee, ...
Friborg, Jacques, Kong, Wing-Pui, Flowers, C. Clay, Flowers, Scarlett L., Sun, Yongnian, Foreman, Kimberly E., ...
The DNA sequence for Kaposi’s sarcoma-associated herpesvirus was originally detected in Kaposi’s sarcoma biopsy specimens. Since its discovery, it has been possible to detect virus in cell lines...
Huang, Yue, Kong, Wing-pui, Nabel, Gary J.
Immunity to human immunodeficiency virus virion-like structures or a polyprotein has been examined after DNA immunization with Rev-independent expression vectors. A Gag-Pol fusion protein stimulated...
Moore, Anne C., Kong, Wing-pui, Chakrabarti, Bimal K., Nabel, Gary J.
The effects of genetic adjuvants on humoral and cell-mediated immunity to two human immunodeficiency virus antigens, Env and Nef, have been examined in mice. Despite similar levels of gene expression...
Baig, Jamal, Levy, Daniel B., McKay, Paul F., Schmitz, Joern E., Santra, Sampa, Subbramanian, Ramu A., ...
Since most human immunodeficiency virus (HIV) infections are initiated following mucosal exposure to the virus, the anatomic containment or abortion of an HIV infection is likely to require...
Chakrabarti, Bimal K., Kong, Wing-pui, Wu, Bei-yue, Yang, Zhi-Yong, Friborg, Jacques, Ling, Xu, ...
In this study, we have investigated the effect of specific mutations in human immunodeficiency virus type 1 (HIV-1) envelope (Env) on antibody production in an effort to improve humoral immune...
Overcoming Immunity to a Viral Vaccine by DNA Priming before Vector Boosting
Yang, Zhi-yong, Wyatt, Linda S., Kong, Wing-pui, Moodie, Zoe, Moss, Bernard, Nabel, Gary J.
Replication-defective adenovirus (ADV) and poxvirus vectors have shown potential as vaccines for pathogens such as Ebola or human immunodeficiency virus in nonhuman primates, but prior immunity to...
Lemiale, Franck, Kong, Wing-pui, Akyürek, Levent M., Ling, Xu, Huang, Yue, Chakrabarti, Bimal K., ...
Replication-defective adenovirus (ADV) vectors represent a promising potential platform for the development of a vaccine for AIDS. Although this vector is typically administered intramuscularly, it...
Immunogenicity of Multiple Gene and Clade Human Immunodeficiency Virus Type 1 DNA Vaccines
Kong, Wing-Pui, Huang, Yue, Yang, Zhi-Yong, Chakrabarti, Bimal K., Moodie, Zoe, Nabel, Gary J.
The ability to elicit an immune response to a spectrum of human immunodeficiency virus type 1 (HIV-1) gene products from divergent strains is a desirable feature of an AIDS vaccine. In this study, we...
Yang, Zhi-yong, Chakrabarti, Bimal K., Xu, Ling, Welcher, Brent, Kong, Wing-pui, Leung, Kwanyee, ...
Although the B clade of human immunodeficiency virus type 1 (HIV-1) envelopes (Env) includes five highly variable regions, each of these domains contains a subset of sequences that remain conserved....
Yang, Zhi-Yong, Huang, Yue, Ganesh, Lakshmanan, Leung, Kwanyee, Kong, Wing-Pui, Schwartz, Owen, ...
The severe acute respiratory syndrome coronavirus (SARS-CoV) synthesizes several putative viral envelope proteins, including the spike (S), membrane (M), and small envelope (E) glycoproteins....
Heterologous Envelope Immunogens Contribute to AIDS Vaccine Protection in Rhesus Monkeys
Letvin, Norman L., Huang, Yue, Chakrabarti, Bimal K., Xu, Ling, Seaman, Michael S., Beaudry, Kristin, ...
Because a strategy to elicit broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies has not yet been found, the role of an Env immunogen in HIV-1 vaccine candidates remains...
Huang, Yue, Yang, Zhi-yong, Kong, Wing-pui, Nabel, Gary J.
The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of...
Mascola, John R., Sambor, Anna, Beaudry, Kristin, Santra, Sampa, Welcher, Brent, Louder, Mark K., ...
Immunization with recombinant serotype 5 adenoviral (rAd5) vectors or a combination of DNA plasmid priming and rAd5 boosting is known to elicit potent immune responses. However, little data exist...
Ebola Virus Glycoprotein Toxicity Is Mediated by a Dynamin-Dependent Protein-Trafficking Pathway
Sullivan, Nancy J., Peterson, Mary, Yang, Zhi-yong, Kong, Wing-pui, Duckers, Heinricus, Nabel, Elizabeth, ...
Ebola virus infection causes a highly lethal hemorrhagic fever syndrome associated with profound immunosuppression through its ability to induce widespread inflammation and cellular damage. Though...
Evasion of antibody neutralization in emerging severe acute respiratory syndrome coronaviruses
Yang, Zhi-yong, Werner, Heidi C., Kong, Wing-pui, Leung, Kwanyee, Traggiai, Elisabetta, Lanzavecchia, Antonio, ...
Molecular characterization of the severe acute respiratory syndrome coronavirus has revealed genetic diversity among isolates. The spike (S) glycoprotein, the major target for vaccine and immune...
Wu, Lan, Kong, Wing-pui, Nabel, Gary J.
A variety of gene-based vaccination approaches have been used to enhance the immune response to viral pathogens. Among them, the ability to perform heterologous immunization by priming with DNA and...
Barouch, Dan H., Yang, Zhi-yong, Kong, Wing-pui, Korioth-Schmitz, Birgit, Sumida, Shawn M., Truitt, Diana M., ...
Plasmid DNA vaccines elicit potent and protective immune responses in numerous small-animal models of infectious diseases. However, their immunogenicity in primates appears less potent. Here we...
Kong, Wing-pui, Xu, Ling, Stadler, Konrad, Ulmer, Jeffrey B., Abrignani, Sergio, Rappuoli, Rino, ...
Although the initial isolates of the severe acute respiratory syndrome (SARS) coronavirus (CoV) are sensitive to neutralization by antibodies through their spike (S) glycoprotein, variants of S have...
Yang, Zhi-yong, Chakrabarti, Bimal K., Xu, Ling, Welcher, Brent, Kong, Wing-pui, Leung, Kwanyee, ...
Mechanism of Ad5 Vaccine Immunity and Toxicity: Fiber Shaft Targeting of Dendritic Cells
Cheng, Cheng, Gall, Jason G. D, Kong, Wing-pui, Sheets, Rebecca L, Gomez, Phillip L, King, C. Richter, ...
Recombinant adenoviral (rAd) vectors elicit potent cellular and humoral immune responses and show promise as vaccines for HIV-1, Ebola virus, tuberculosis, malaria, and other infections. These...
Protective immunity to lethal challenge of the 1918 pandemic influenza virus by vaccination
Kong, Wing-pui, Hood, Chantelle, Yang, Zhi-yong, Wei, Chih-Jen, Xu, Ling, García-Sastre, Adolfo, ...
The remarkable infectivity and virulence of the 1918 influenza virus resulted in an unprecedented pandemic, raising the question of whether it is possible to develop protective immunity to this virus...
Wei, Chih-Jen, Xu, Ling, Kong, Wing-Pui, Shi, Wei, Canis, Kevin, Stevens, James, ...
Although the human transmission of avian H5N1 virus remains low, the prevalence of this highly pathogenic infection in avian species underscores the need for a preventive vaccine that can be made...
Ko, Sung-Youl, Cheng, Cheng, Kong, Wing-Pui, Wang, Lingshu, Kanekiyo, Masaru, Einfeld, David, ...
Human immunodeficiency virus type 1 (HIV-1) infection is characterized by the rapid onset of intestinal T-cell depletion that initiates the progression to AIDS. The induction of protective immunity...
Kong, Wing-Pui, Wu, Lan, Wallstrom, Timothy C., Fischer, Will, Yang, Zhi-Yong, Ko, Sung-Youl, ...
An effective AIDS vaccine must control highly diverse circulating strains of human immunodeficiency virus type 1 (HIV-1). Among HIV-1 gene products, the envelope (Env) protein contains variable as...