Burkholderia pseudomalleigenome plasticity associated with genomic island variation (2008)
Tumapa, Sarinna, Holden, Matthew TG, Vesaratchavest, Mongkol, Wuthiekanun, Vanaporn, Limmathurotsakul, Direk, Chierakul, Wirongrong, ...
Abstract Background Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene transfer contributes to the genetic diversity of this pathogen and may be an...
W. Joost Wiersinga, Catharina W. Wieland, Mark C. Dessing, Narisara Chantratita, Allen C. Cheng, Direk Limmathurotsakul, ...
BackgroundToll-like receptors (TLRs) are essential in host defense against pathogens by virtue of their capacity to detect microbes and initiate the immune response. TLR2 is seen as the most...
Wuthiekanun, Vanaporn, Amornchai, Premjit, Chierakul, Wirongrong, Cheng, Allen C., White, Nicholas J., Peacock, Sharon J., ...
An enzyme-linked immunosorbent assay-based rapid cassette immunoglobulin G (IgG) and IgM immunochromogenic test kit was compared to the indirect hemagglutination test (IHA) for the diagnosis of acute...
Detection of Burkholderia pseudomallei in Soil within the Lao People's Democratic Republic
Wuthiekanun, Vanaporn, Mayxay, Mayfong, Chierakul, Wirongrong, Phetsouvanh, Rattanaphone, Cheng, Allen C., White, Nicholas J, ...
Clinical cases of melioidosis caused by the saprophyte Burkholderia pseudomallei were first noted in the Lao People's Democratic Republic (PDR) in 1999. In this study, 36% of 110 soil samples in...
Pharmacokinetics of Oral Doxycycline during Combination Treatment of Severe Falciparum Malaria
Newton, Paul N., Chaulet, Jean-François, Brockman, Alan, Chierakul, Wirongrong, Dondorp, Arjen, Ruangveerayuth, Ronatrai, ...
The pharmacokinetics of oral doxycycline administered at 200 mg every 24 h were investigated in 17 patients recovering from severe Plasmodium falciparum malaria. The data suggest that the doses of...
Rapid Immunofluorescence Microscopy for Diagnosis of Melioidosis
Wuthiekanun, Vanaporn, Desakorn, Varunee, Wongsuvan, Gumphol, Amornchai, Premjit, Cheng, Allen C., Maharjan, Bina, ...
An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were...
Role and Significance of Quantitative Urine Cultures in Diagnosis of Melioidosis
Limmathurotsakul, Direk, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Cheng, Allen C., Maharjan, Bina, Chaowagul, Wipada, ...
Melioidosis is associated with significant mortality in countries in which it is endemic. Previous studies have demonstrated that quantitative Burkholderia pseudomallei counts in blood are predictive...
Tiyawisutsri, Rachaneeporn, Peacock, Sharon J., Langa, Sayan, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, ...
The indirect hemagglutination assay routinely used to detect antibodies to Burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to B. pseudomallei, B. mallei, and B....
Peacock, Sharon J., Chieng, Grace, Cheng, Allen C., Dance, David A. B., Amornchai, Premjit, Wongsuvan, Gumphol, ...
Ashdown's medium, Burkholderia pseudomallei selective agar (BPSA), and a commercial Burkholderia cepacia medium were compared for their abilities to grow B. pseudomallei from 155 clinical specimens...
Chaowagul, Wipada, Chierakul, Wirongrong, Simpson, Andrew J., Short, Jennifer M., Stepniewska, Kasia, Maharjan, Bina, ...
Melioidosis (infection caused by Burkholderia pseudomallei) requires a prolonged course of oral antibiotics following initial intravenous therapy to reduce the risk of relapse after cessation of...
Maharjan, Bina, Chantratita, Narisara, Vesaratchavest, Mongkol, Cheng, Allen, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, ...
Human melioidosis is associated with a high rate of recurrent disease, despite adequate antimicrobial treatment. Here, we define the rate of relapse versus the rate of reinfection in 116 patients...
Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Rattanalertnavee, Jurairat, Langa, Sayan, Sirodom, Damrong, Wattanawaitunechai, Charnkij, ...
A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December...
Vesaratchavest, Mongkol, Tumapa, Sarinna, Day, Nicholas P. J., Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Holden, Matthew T. G., ...
Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern...
Wuthiekanun, Vanaporn, Amornchai, Premjit, Chierakul, Wirongrong, Cheng, Allen C., White, Nicholas J., Peacock, Sharon J., ...
An enzyme-linked immunosorbent assay-based rapid cassette immunoglobulin G (IgG) and IgM immunochromogenic test kit was compared to the indirect hemagglutination test (IHA) for the diagnosis of acute...
Detection of Burkholderia pseudomallei in Soil within the Lao People's Democratic Republic
Wuthiekanun, Vanaporn, Mayxay, Mayfong, Chierakul, Wirongrong, Phetsouvanh, Rattanaphone, Cheng, Allen C., White, Nicholas J, ...
Clinical cases of melioidosis caused by the saprophyte Burkholderia pseudomallei were first noted in the Lao People's Democratic Republic (PDR) in 1999. In this study, 36% of 110 soil samples in...
Pharmacokinetics of Oral Doxycycline during Combination Treatment of Severe Falciparum Malaria
Newton, Paul N., Chaulet, Jean-François, Brockman, Alan, Chierakul, Wirongrong, Dondorp, Arjen, Ruangveerayuth, Ronatrai, ...
The pharmacokinetics of oral doxycycline administered at 200 mg every 24 h were investigated in 17 patients recovering from severe Plasmodium falciparum malaria. The data suggest that the doses of...
Rapid Immunofluorescence Microscopy for Diagnosis of Melioidosis
Wuthiekanun, Vanaporn, Desakorn, Varunee, Wongsuvan, Gumphol, Amornchai, Premjit, Cheng, Allen C., Maharjan, Bina, ...
An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were...
Role and Significance of Quantitative Urine Cultures in Diagnosis of Melioidosis
Limmathurotsakul, Direk, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Cheng, Allen C., Maharjan, Bina, Chaowagul, Wipada, ...
Melioidosis is associated with significant mortality in countries in which it is endemic. Previous studies have demonstrated that quantitative Burkholderia pseudomallei counts in blood are predictive...
Tiyawisutsri, Rachaneeporn, Peacock, Sharon J., Langa, Sayan, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, ...
The indirect hemagglutination assay routinely used to detect antibodies to Burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to B. pseudomallei, B. mallei, and B....
Peacock, Sharon J., Chieng, Grace, Cheng, Allen C., Dance, David A. B., Amornchai, Premjit, Wongsuvan, Gumphol, ...
Ashdown's medium, Burkholderia pseudomallei selective agar (BPSA), and a commercial Burkholderia cepacia medium were compared for their abilities to grow B. pseudomallei from 155 clinical specimens...
Chaowagul, Wipada, Chierakul, Wirongrong, Simpson, Andrew J., Short, Jennifer M., Stepniewska, Kasia, Maharjan, Bina, ...
Melioidosis (infection caused by Burkholderia pseudomallei) requires a prolonged course of oral antibiotics following initial intravenous therapy to reduce the risk of relapse after cessation of...
Maharjan, Bina, Chantratita, Narisara, Vesaratchavest, Mongkol, Cheng, Allen, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, ...
Human melioidosis is associated with a high rate of recurrent disease, despite adequate antimicrobial treatment. Here, we define the rate of relapse versus the rate of reinfection in 116 patients...
Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Rattanalertnavee, Jurairat, Langa, Sayan, Sirodom, Damrong, Wattanawaitunechai, Charnkij, ...
A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December...
Vesaratchavest, Mongkol, Tumapa, Sarinna, Day, Nicholas P. J., Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Holden, Matthew T. G., ...
Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern...
Chantratita, Narisara, Wuthiekanun, Vanaporn, Thanwisai, Aunchalee, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, ...
Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast...
Biological Relevance of Colony Morphology and Phenotypic Switching by Burkholderia pseudomallei▿
Chantratita, Narisara, Wuthiekanun, Vanaporn, Boonbumrung, Khaemaporn, Tiyawisutsri, Rachaneeporn, Vesaratchavest, Mongkol, Limmathurotsakul, Direk, ...
Melioidosis is a notoriously protracted illness and is difficult to cure. We hypothesize that the causative organism, Burkholderia pseudomallei, undergoes a process of adaptation involving altered...
Brouwer, Annemarie E., Johnson, Elizabeth, Rajanuwong, Adul, Teparrukkul, Prapit, Wuthiekanun, Vannaporn, ...
In a randomized controlled trial of amphotericin B-based therapy for human immunodeficiency virus (HIV)-associated cryptococcal meningitis in Thailand, we also compared the mycological efficacy,...
Optimization of Culture of Leptospira from Humans with Leptospirosis▿
Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Limmathurotsakul, Direk, Smythe, Lee D., Symonds, Meegan L., Dohnt, Michael F., ...
A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison...
Wiersinga, W. Joost, Wieland, Catharina W, Dessing, Mark C, Chantratita, Narisara, Cheng, Allen C, Limmathurotsakul, Direk, ...
Willem Wiersinga and colleagues find up-regulation of multiple Toll-like receptors (TLRs) in peripheral blood cells of patients with melioidosis. However, only TLR2 had an effect on the immune...
Thaipadungpanit, Janjira, Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Smythe, Lee D., Petkanchanapong, Wimol, Limpaiboon, Roongrueng, ...
A sustained outbreak of human leptospirosis occurred in northeast Thailand between 1999 and 2003, the basis for which was unknown. Leptospirosis is a potentially serious infection cause by bacteria...
Doxycycline versus Azithromycin for Treatment of Leptospirosis and Scrub Typhus▿
Phimda, Kriangsak, Hoontrakul, Siriwan, Suttinont, Chuanpit, Chareonwat, Sompong, Losuwanaluk, Kitti, Chueasuwanchai, Sunee, ...
Leptospirosis and scrub typhus are important causes of acute fever in Southeast Asia. Options for empirical therapy include doxycycline and azithromycin, but it is unclear whether their efficacies...
Amornchai, Premjit, Chierakul, Wirongrong, Wuthiekanun, Vanaporn, Mahakhunkijcharoen, Yuvadee, Phetsouvanh, Rattanaphone, Currie, Bart J., ...
In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei...