Access the most recent version at doi: 10.1110/ps.041148605 References (2008)
Wolfgang Baumeister, Email Alerting, Wolfgang Baumeister
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MAPPING MOLECULAR LANDSCAPES INSIDE CELLS BY CRYOELECTRON TOMOGRAPHY (2008)
Electron Tomography (ET) is uniquely suited to obtain three-dimensional (3-D) images of large pleiomorphic structures, such as organelles or even whole cells. While the principles of ET have been...
Felix Hoppe-Seyler Lecture 2004 Mapping molecular landscapes inside cells (2008)
Cryoelectron tomography opens a window into the inner space of cells. It combines the potential of three-dimensional imaging with a close-to-life preservation of biological samples. Tomograms with...
An idea whose time has come (2007)
Banci, Lucia, Baumeister, Wolfgang, Heinemann, Udo, Schneider, Gunter, Silman, Israel, Stuart, David I, ...
A response to An idea whose time has gone by Gregory A Petsko, Genome Biology 2007, 8 :107.
Nickell, Stephan, Park, Paul S.-H., Baumeister, Wolfgang, Palczewski, Krzysztof
The rod outer segment (ROS) of photoreceptor cells houses all components necessary for phototransduction, a set of biochemical reactions that amplify and propagate a light signal. Theoretical...
Cryo-electron tomography of biological specimens (2006)
Leis, Andrew P., Beck, Martin, Gruska, Manuela, Best, Christoph, Hegerl, Reiner, Baumeister, Wolfgang, ...
This paper describes the important considerations in cryo-electron tomography (CET) of biological specimens and identifies the areas where digital signal processing can make a decisive contribution.
Cryo-electron tomography of biological specimens (2006)
Leis, Andrew P., Beck, Martin, Gruska, Manuela, Best, Christoph, Hegerl, Reiner, Baumeister, Wolfgang, ...
This paper describes the important considerations in cryo-electron tomography (CET) of biological specimens and identifies the areas where digital signal processing can make a decisive contribution.
Cryo-electron tomography of biological specimens (2006)
Leis, Andrew P., Beck, Martin, Gruska, Manuela, Best, Christoph, Hegerl, Reiner, Baumeister, Wolfgang, ...
This paper describes the important considerations in cryo-electron tomography (CET) of biological specimens and identifies the areas where digital signal processing can make a decisive contribution.
Cryo-electron tomography of biological specimens (2006)
Leis, Andrew P., Beck, Martin, Gruska, Manuela, Best, Christoph, Hegerl, Reiner, Baumeister, Wolfgang, ...
This paper describes the important considerations in cryo-electron tomography (CET) of biological specimens and identifies the areas where digital signal processing can make a decisive contribution.
Cryo-electron tomography of biological specimens (2006)
Leis, Andrew P., Beck, Martin, Gruska, Manuela, Best, Christoph, Hegerl, Reiner, Baumeister, Wolfgang, ...
This paper describes the important considerations in cryo-electron tomography (CET) of biological specimens and identifies the areas where digital signal processing can make a decisive contribution.
Cryo-electron tomography of biological specimens (2006)
Leis, Andrew P., Beck, Martin, Gruska, Manuela, Best, Christoph, Hegerl, Reiner, Baumeister, Wolfgang, ...
This paper describes the important considerations in cryo-electron tomography (CET) of biological specimens and identifies the areas where digital signal processing can make a decisive contribution.
Tamura, Tomohiro, Schoofs, Geert, ...
In a proteasome-lacking mutant of Streptomyces coelicolor A3(2), an intracellular enzyme with chymotrypsin-like activity, absent from the wild type, was detected. Complementation that restored...
The 20S Proteasome of Streptomyces coelicolor (1998)
István Nagy, Tomohiro Tamura, Jos Vanderleyden, Wolfgang Baumeister, René De Mot
20S proteasomes were purified from Streptomyces coelicolor A3(2) and shown to be built from one �-type subunit (PrcA) and one �-type subunit (PrcB). The enzyme displayed chymotrypsin-like...
Energy filtered electron tomography of iceembedded actin and vesicles (1997)
Rudo Grimm, Michael Bdrmann, Walter Hackl, Dieter Typke, Erich Sackmann, Wolfgang Baumeister
ABSTRACT Semiautomatic single-axis tilt electron tomography has been used to visualize the three-dimensional organization of actin filaments in "phantom cells, " i.e. lipid...
Düsseldorf, Univ., Math.-Naturwiss. Fak., Diss., 1973.
N-Halogen-Silylamine : massenspektroskopische Untersuchungen an Trimethylsilylverbindungen / (1971)
Thesis (doctoral)--Ludwig-Maximilians Universität München, 1971.
Tübingen, Fachbereich Geschichte-Geographie, Diss. v. 25. Aug. 1970.
Morphologische Untersuchungen zum Drüsengewicht und Gangsystem des menschlichen Pankreas / (1968)
Thesis (doctoral)--Universität Hamburg, 1968.
Böhm, Jochen, Frangakis, Achilleas S., Hegerl, Reiner, Nickell, Stephan, Typke, Dieter, Baumeister, Wolfgang
Electron tomography is the only technique available that allows us to visualize the three-dimensional structure of unfixed and unstained cells currently with a resolution of 6–8 nm, but with the...
Controlled unzipping of a bacterial surface layer with atomic force microscopy
Müller, Daniel J., Baumeister, Wolfgang, Engel, Andreas
We have combined high-resolution atomic force microscopy (AFM) imaging and force spectroscopy to gain insight into the interaction forces between the individual protomers of the hexagonally packed...
The 20S Proteasome of Streptomyces coelicolor
Nagy, István, Tamura, Tomohiro, Vanderleyden, Jos, Baumeister, Wolfgang, De Mot, René
20S proteasomes were purified from Streptomyces coelicolor A3(2) and shown to be built from one α-type subunit (PrcA) and one β-type subunit (PrcB). The enzyme displayed chymotrypsin-like activity...
A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy
Rockel, Beate, Peters, Jürgen, Kühlmorgen, Brigitte, Glaeser, Robert M., Baumeister, Wolfgang
Tripeptidyl peptidase II (TPP II) is an exopeptidase of the subtilisin type of serine proteases that is thought to act downstream of the proteasome in the ubiquitin–proteasome pathway. Recently,...
Identification of macromolecular complexes in cryoelectron tomograms of phantom cells
Frangakis, Achilleas S., Böhm, Jochen, Förster, Friedrich, Nickell, Stephan, Nicastro, Daniela, Typke, Dieter, ...
Electron tomograms of intact frozen-hydrated cells are essentially three-dimensional images of the entire proteome of the cell, and they depict the whole network of macromolecular interactions....
Nagy, István, Banerjee, Tisha, Tamura, Tomohiro, Schoofs, Geert, Gils, Ann, Proost, Paul, ...
In a proteasome-lacking mutant of Streptomyces coelicolor A3(2), an intracellular enzyme with chymotrypsin-like activity, absent from the wild type, was detected. Complementation that restored...
Stoichiometric model of the photosynthetic unit of Ectothiorhodospira halochloris
Engelhardt, Harald, Engel, Andreas, Baumeister, Wolfgang
A stoichiometric model of the photosynthetic unit of Ectothiorhodospira halochloris has been obtained by means of scanning transmission electron microscope mass determination and mass mapping in...
Kessel, Martin, Wildhaber, Ivo, Cohen, Simone, Baumeister, Wolfgang
A three-dimensional reconstruction from electron micrographs of negatively stained cell envelopes of Halobacterium volcanii has revealed the structure of the surface glycoprotein to a resolution of 2...
Cryo-electron tomography of vaccinia virus
Cyrklaff, Marek, Risco, Cristina, Fernández, Jose Jesús, Jiménez, Maria Victoria, Estéban, Mariano, Baumeister, Wolfgang, ...
The combination of cryo-microscopy and electron tomographic reconstruction has allowed us to determine the structure of one of the more complex viruses, intracellular mature vaccinia virus, at a...
Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography
Förster, Friedrich, Medalia, Ohad, Zauberman, Nathan, Baumeister, Wolfgang, Fass, Deborah
We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murine...
Molecular architecture and assembly mechanism of Drosophila tripeptidyl peptidase II
Rockel, Beate, Peters, Jürgen, Müller, Shirley A., Seyit, Gönül, Ringler, Philippe, Hegerl, Reiner, ...
In eukaryotes, tripeptidyl peptidase II (TPPII) is a crucial component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It is an amino peptidase...
Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements.
Kurucz, Eva, Andó, István, Sümegi, Máté, Hölzl, Harald, Kapelari, Barbara, Baumeister, Wolfgang, ...
The subunit contacts in the regulatory complex of the Drosophila 26 S proteasome were studied through the cross-linking of closely spaced subunits of the complex, and analysis of the cross-linking...
3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography
Nicastro, Daniela, McIntosh, J. Richard, Baumeister, Wolfgang
We have used cryo-electron tomography to investigate the 3D structure and macromolecular organization of intact, frozen-hydrated sea urchin sperm flagella in a quiescent state. The tomographic...
Böhm, Jochen, Frangakis, Achilleas S., Hegerl, Reiner, Nickell, Stephan, Typke, Dieter, Baumeister, Wolfgang
Electron tomography is the only technique available that allows us to visualize the three-dimensional structure of unfixed and unstained cells currently with a resolution of 6–8 nm, but with the...
Controlled unzipping of a bacterial surface layer with atomic force microscopy
Müller, Daniel J., Baumeister, Wolfgang, Engel, Andreas
We have combined high-resolution atomic force microscopy (AFM) imaging and force spectroscopy to gain insight into the interaction forces between the individual protomers of the hexagonally packed...
The 20S Proteasome of Streptomyces coelicolor
Nagy, István, Tamura, Tomohiro, Vanderleyden, Jos, Baumeister, Wolfgang, De Mot, René
20S proteasomes were purified from Streptomyces coelicolor A3(2) and shown to be built from one α-type subunit (PrcA) and one β-type subunit (PrcB). The enzyme displayed chymotrypsin-like activity...
A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy
Rockel, Beate, Peters, Jürgen, Kühlmorgen, Brigitte, Glaeser, Robert M., Baumeister, Wolfgang
Tripeptidyl peptidase II (TPP II) is an exopeptidase of the subtilisin type of serine proteases that is thought to act downstream of the proteasome in the ubiquitin–proteasome pathway. Recently,...
Identification of macromolecular complexes in cryoelectron tomograms of phantom cells
Frangakis, Achilleas S., Böhm, Jochen, Förster, Friedrich, Nickell, Stephan, Nicastro, Daniela, Typke, Dieter, ...
Electron tomograms of intact frozen-hydrated cells are essentially three-dimensional images of the entire proteome of the cell, and they depict the whole network of macromolecular interactions....
Nagy, István, Banerjee, Tisha, Tamura, Tomohiro, Schoofs, Geert, Gils, Ann, Proost, Paul, ...
In a proteasome-lacking mutant of Streptomyces coelicolor A3(2), an intracellular enzyme with chymotrypsin-like activity, absent from the wild type, was detected. Complementation that restored...
Stoichiometric model of the photosynthetic unit of Ectothiorhodospira halochloris
Engelhardt, Harald, Engel, Andreas, Baumeister, Wolfgang
A stoichiometric model of the photosynthetic unit of Ectothiorhodospira halochloris has been obtained by means of scanning transmission electron microscope mass determination and mass mapping in...
Kessel, Martin, Wildhaber, Ivo, Cohen, Simone, Baumeister, Wolfgang
A three-dimensional reconstruction from electron micrographs of negatively stained cell envelopes of Halobacterium volcanii has revealed the structure of the surface glycoprotein to a resolution of 2...
Cryo-electron tomography of vaccinia virus
Cyrklaff, Marek, Risco, Cristina, Fernández, Jose Jesús, Jiménez, Maria Victoria, Estéban, Mariano, Baumeister, Wolfgang, ...
The combination of cryo-microscopy and electron tomographic reconstruction has allowed us to determine the structure of one of the more complex viruses, intracellular mature vaccinia virus, at a...
Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography
Förster, Friedrich, Medalia, Ohad, Zauberman, Nathan, Baumeister, Wolfgang, Fass, Deborah
We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murine...
Molecular architecture and assembly mechanism of Drosophila tripeptidyl peptidase II
Rockel, Beate, Peters, Jürgen, Müller, Shirley A., Seyit, Gönül, Ringler, Philippe, Hegerl, Reiner, ...
In eukaryotes, tripeptidyl peptidase II (TPPII) is a crucial component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. It is an amino peptidase...
Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements.
Kurucz, Eva, Andó, István, Sümegi, Máté, Hölzl, Harald, Kapelari, Barbara, Baumeister, Wolfgang, ...
The subunit contacts in the regulatory complex of the Drosophila 26 S proteasome were studied through the cross-linking of closely spaced subunits of the complex, and analysis of the cross-linking...
3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography
Nicastro, Daniela, McIntosh, J. Richard, Baumeister, Wolfgang
We have used cryo-electron tomography to investigate the 3D structure and macromolecular organization of intact, frozen-hydrated sea urchin sperm flagella in a quiescent state. The tomographic...
Whole Cell Cryo-Electron Tomography Reveals Distinct Disassembly Intermediates of Vaccinia Virus
Cyrklaff, Marek, Linaroudis, Alexandros, Boicu, Marius, Chlanda, Petr, Baumeister, Wolfgang, Griffiths, Gareth, ...
At each round of infection, viruses fall apart to release their genome for replication, and then reassemble into stable particles within the same host cell. For most viruses, the structural details...
Luminal particles within cellular microtubules
Garvalov, Boyan K., Zuber, Benoît, Bouchet-Marquis, Cédric, Kudryashev, Mikhail, Gruska, Manuela, Beck, Martin, ...
The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind to the microtubule outer surface, but little is known about cellular components that may associate...
Three-dimensional architecture of murine rod outer segments determined by cryoelectron tomography
Nickell, Stephan, Park, Paul S.-H., Baumeister, Wolfgang, Palczewski, Krzysztof
The rod outer segment (ROS) of photoreceptor cells houses all components necessary for phototransduction, a set of biochemical reactions that amplify and propagate a light signal. Theoretical...
Cyrklaff, Marek, Kudryashev, Mikhail, Leis, Andrew, Leonard, Kevin, Baumeister, Wolfgang, Menard, Robert, ...
Microtubules are dynamic cytoskeletal structures important for cell division, polarity, and motility and are therefore major targets for anticancer and antiparasite drugs. In the invasive forms of...
Banci, Lucia, Baumeister, Wolfgang, Heinemann, Udo, Schneider, Gunter, Silman, Israel, Stuart, David I, ...
A response to An idea whose time has gone by Gregory A Petsko, Genome Biology 2007, 8:107.
Niedermann, Gabriele, Grimm, Rudolf, Geier, Elke, Maurer, Martina, Realini, Claudio, Gartmann, Christoph, ...
To generate peptides for presentation by major histocompatibility complex (MHC) class I molecules to T lymphocytes, the immune system of vertebrates has recruited the proteasomes, phylogenetically...
Hölzl, Harald, Kapelari, Barbara, Kellermann, Josef, Seemüller, Erika, Sümegi, Máté, Udvardy, Andor, ...
Drosophila melanogaster embryos are a source for homogeneous and stable 26S proteasomes suitable for structural studies. For biochemical characterization, purified 26S proteasomes were resolved by...
Xylanase Attachment to the Cell Wall of the Hyperthermophilic Bacterium Thermotoga maritima▿
Liebl, Wolfgang, Winterhalter, Christoph, Baumeister, Wolfgang, Armbrecht, Martin, Valdez, Michael
The cellular localization and processing of the endo-xylanases (1,4-β-d-xylan-xylanohydrolase; EC 3.2.1.8) of the hyperthermophile Thermotoga maritima were investigated, in particular with respect...
Cryo-electron tomography of cells: connecting structure and function
Lučić, Vladan, Leis, Andrew, Baumeister, Wolfgang
Cryo-electron tomography (cryo-ET) allows the visualization of cellular structures under close-to-life conditions and at molecular resolution. While it is inherently a static approach, yielding...