Choe, WS, Clemmitt, RH, Chase, HA, Middelberg, APJ
Integration of the chemical extraction of recombinant inclusion-body protein from Escherichia coli, and its recovery by metal-affinity expanded-bed adsorption (IMAC-EBA) under denaturing conditions,...
The economics of inclusion body processing
Lee, GH, Cooney, D, Middelberg, APJ, Choe, WS
Many recombinant proteins are often over-expressed in host cells, such as Escherichia coli, and are found as insoluble and inactive protein aggregates known as inclusion bodies (IBs). Recently, a...