Bos taurusgenome assembly (2009)
Liu, Yue, Qin, Xiang, Song, Xing-Zhi, Jiang, Huaiyang, Shen, Yufeng, Durbin, K James, ...
Abstract Background We present here the assembly of the bovine genome. The assembly method combines the BAC plus WGS local assembly used for the rat and sea urchin with the whole genome shotgun (WGS)...
Bourgogne, Agathe, Garsin, Danielle A, Qin, Xiang, Singh, Kavindra V, Sillanpaa, Jouko, Yerrapragada, Shailaja, ...
Abstract Background Enterococcus faecalis has emerged as a major hospital pathogen. To explore its diversity, we sequenced E. faecalis strain OG1RF, which is commonly used for molecular manipulation...
Highlander, Sarah K, Hultén, Kristina G, Qin, Xiang, Jiang, Huaiyang, Yerrapragada, Shailaja, Mason, Edward O, ...
Abstract Background Community acquired (CA) methicillin-resistant Staphylococcus aureus (MRSA) increasingly causes disease worldwide. USA300 has emerged as the predominant clone causing superficial...
Michael P. Mcleod, Xiang Qin, Or E. Karpathy, Jason Gioia, Sarah K. Highl, George E. Fox, ...
Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome...
Identification and characterization of virulence factors in Enterococcus faecalis (2000)
Enterococci are one of the leading causes of nosocomial infections, and Enterococcus faecalis causes the majority of enterococcal infections. However, the mechanisms of enterococcal pathogenesis are...
A simple model host for identifying Gram-positive virulence factors
Garsin, Danielle A., Sifri, Costi D., Mylonakis, Eleftherios, Qin, Xiang, Singh, Kavindra V., Murray, Barbara E., ...
We demonstrate the use of the nematode Caenorhabditis elegans as a facile and inexpensive model host for several Gram-positive human bacterial pathogens. Enterococcus faecalis, Streptococcus...
Analysis of a Gene Cluster of Enterococcus faecalis Involved in Polysaccharide Biosynthesis
Xu, Yi, Singh, Kavindra V., Qin, Xiang, Murray, Barbara E., Weinstock, George M.
Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E....
Qin, Xiang, Singh, Kavindra V., Weinstock, George M., Murray, Barbara E.
Three agr-like genes (fsrA, fsrB, and fsrC, for Enterococcus faecalis regulator) were found upstream of the previously reported gelatinase gene (gelE) and a downstream putative serine protease gene...
Qin, Xiang, Singh, Kavindra V., Weinstock, George M., Murray, Barbara E.
We have previously identified a locus, fsr, a homologue of staphylococcal agr loci, which positively regulates the expression of gelatinase and serine protease (encoded by gelE and sprE,...
Nallapareddy, Sreedhar R., Qin, Xiang, Weinstock, George M., Höök, Magnus, Murray, Barbara E.
Adhesin-mediated binding to extracellular matrix (ECM) proteins is thought to be a crucial step in the pathogenic process of many bacterial infections. We have previously reported conditional...
Effect of Disruption of a Gene Encoding an Autolysin of Enterococcus faecalis OG1RF
Qin, Xiang, Singh, Kavindra V., Xu, Yi, Weinstock, George M., Murray, Barbara E.
A mutant (TX5127) of Enterococcus faecalis OG1RF was generated by disruption mutagenesis of a previously described autolysin gene. TX5127 formed longer chains (2 to 10 cells per chain) than wild-type...
Mylonakis, Eleftherios, Engelbert, Michael, Qin, Xiang, Sifri, Costi D., Murray, Barbara E., Ausubel, Frederick M., ...
We used a rabbit endophthalmitis model to explore the role of fsrB, a gene required for the function of the fsr quorum-sensing system of Enterococcus faecalis, in pathogenicity. A nonpolar deletion...
Sifri, Costi D., Mylonakis, Eleftherios, Singh, Kavindra V., Qin, Xiang, Garsin, Danielle A., Murray, Barbara E., ...
The expression of two Enterococcus faecalis extracellular virulence-related proteins, gelatinase (GelE) and serine protease (SprE), has been shown to be positively regulated by the fsr quorum-sensing...
Complete Genome Sequence of Rickettsia typhi and Comparison with Sequences of Other Rickettsiae
McLeod, Michael P., Qin, Xiang, Karpathy, Sandor E., Gioia, Jason, Highlander, Sarah K., Fox, George E., ...
Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome...
A simple model host for identifying Gram-positive virulence factors
Garsin, Danielle A., Sifri, Costi D., Mylonakis, Eleftherios, Qin, Xiang, Singh, Kavindra V., Murray, Barbara E., ...
We demonstrate the use of the nematode Caenorhabditis elegans as a facile and inexpensive model host for several Gram-positive human bacterial pathogens. Enterococcus faecalis, Streptococcus...
Analysis of a Gene Cluster of Enterococcus faecalis Involved in Polysaccharide Biosynthesis
Xu, Yi, Singh, Kavindra V., Qin, Xiang, Murray, Barbara E., Weinstock, George M.
Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E....
Qin, Xiang, Singh, Kavindra V., Weinstock, George M., Murray, Barbara E.
Three agr-like genes (fsrA, fsrB, and fsrC, for Enterococcus faecalis regulator) were found upstream of the previously reported gelatinase gene (gelE) and a downstream putative serine protease gene...
Qin, Xiang, Singh, Kavindra V., Weinstock, George M., Murray, Barbara E.
We have previously identified a locus, fsr, a homologue of staphylococcal agr loci, which positively regulates the expression of gelatinase and serine protease (encoded by gelE and sprE,...
Nallapareddy, Sreedhar R., Qin, Xiang, Weinstock, George M., Höök, Magnus, Murray, Barbara E.
Adhesin-mediated binding to extracellular matrix (ECM) proteins is thought to be a crucial step in the pathogenic process of many bacterial infections. We have previously reported conditional...
Effect of Disruption of a Gene Encoding an Autolysin of Enterococcus faecalis OG1RF
Qin, Xiang, Singh, Kavindra V., Xu, Yi, Weinstock, George M., Murray, Barbara E.
A mutant (TX5127) of Enterococcus faecalis OG1RF was generated by disruption mutagenesis of a previously described autolysin gene. TX5127 formed longer chains (2 to 10 cells per chain) than wild-type...
Mylonakis, Eleftherios, Engelbert, Michael, Qin, Xiang, Sifri, Costi D., Murray, Barbara E., Ausubel, Frederick M., ...
We used a rabbit endophthalmitis model to explore the role of fsrB, a gene required for the function of the fsr quorum-sensing system of Enterococcus faecalis, in pathogenicity. A nonpolar deletion...
Sifri, Costi D., Mylonakis, Eleftherios, Singh, Kavindra V., Qin, Xiang, Garsin, Danielle A., Murray, Barbara E., ...
The expression of two Enterococcus faecalis extracellular virulence-related proteins, gelatinase (GelE) and serine protease (SprE), has been shown to be positively regulated by the fsr quorum-sensing...
Complete Genome Sequence of Rickettsia typhi and Comparison with Sequences of Other Rickettsiae
McLeod, Michael P., Qin, Xiang, Karpathy, Sandor E., Gioia, Jason, Highlander, Sarah K., Fox, George E., ...
Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome...
Gioia, Jason, Qin, Xiang, Jiang, Huaiyang, Clinkenbeard, Kenneth, Lo, Reggie, Liu, Yamei, ...
The draft genome sequence of Mannheimia haemolytica A1, the causative agent of bovine respiratory disease complex (BRDC), is presented. Strain ATCC BAA-410, isolated from the lung of a calf with...
Karpathy, Sandor E., Qin, Xiang, Gioia, Jason, Jiang, Huaiyang, Liu, Yamei, Petrosino, Joseph F., ...
Fusobacterium nucleatum is a prominent member of the oral microbiota and is a common cause of human infection. F. nucleatum includes five subspecies: polymorphum, nucleatum, vincentii, fusiforme, and...
Paradoxical DNA Repair and Peroxide Resistance Gene Conservation in Bacillus pumilus SAFR-032
Gioia, Jason, Yerrapragada, Shailaja, Qin, Xiang, Jiang, Huaiyang, Igboeli, Okezie C., Muzny, Donna, ...
Highlander, Sarah K, Hultén, Kristina G, Qin, Xiang, Jiang, Huaiyang, Yerrapragada, Shailaja, Mason, Edward O, ...
Durfee, Tim, Nelson, Richard, Baldwin, Schuyler, Plunkett, Guy, Burland, Valerie, Mau, Bob, ...
Escherichia coli DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and...
Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF
Bourgogne, Agathe, Garsin, Danielle A, Qin, Xiang, Singh, Kavindra V, Sillanpaa, Jouko, Yerrapragada, Shailaja, ...
A comparison of two strains of the hospital pathogen Enterococcus faecalis suggests that mediators of virulence differ between strains and that virulence does not depend on mobile gene elements