Streptococcus pneumoniae gyrA and gyrB genes specifying the DNA gyrase subunits have been cloned into pET plasmid vectors under the control of an inducible T7 promoter and have been separately...
Alovero, Fabiana L., Pan, Xiao-Su, Morris, Julia E., Manzo, Ruben H., Fisher, L. Mark
We have examined the antipneumococcal mechanisms of a series of novel fluoroquinolones that are identical to ciprofloxacin except for the addition of a benzenesulfonylamido group to the C-7...
Pan, Xiao-Su, Yague, Genoveva, Fisher, L. Mark
Mutations in DNA gyrase and/or topoisomerase IV genes are frequently encountered in quinolone-resistant mutants of Streptococcus pneumoniae. To investigate the mechanism of their effects at the...
DNA Gyrase and Topoisomerase IV Are Dual Targets of Clinafloxacin Action in Streptococcus pneumoniae
We examined the response of Streptococcus pneumoniae 7785 to clinafloxacin, a novel C-8-substituted fluoroquinolone which is being developed as an antipneumococcal agent. Clinafloxacin was highly...
Morris, Julia E., Pan, Xiao-Su, Fisher, L. Mark
Grepafloxacin, a 5-methyl-7-piperazinyl-3"-methyl analogue of ciprofloxacin, was used to obtain stepwise-selected mutants of Streptococcus pneumoniae 7785. Analysis of the quinolone...
Yague, Genoveva, Morris, Julia E., Pan, Xiao-Su, Gould, Katherine A., Fisher, L. Mark
Gemifloxacin is a recently developed fluoroquinolone with potent activity against Streptococcus pneumoniae. We show that the drug is more active than moxifloxacin, gatifloxacin, levofloxacin, and...
Pan, Xiao-Su, Hamlyn, Penelope J., Talens-Visconti, Raquel, Alovero, Fabiana L., Manzo, Ruben H., Fisher, L. Mark
Fluoroquinolones acting equally through DNA gyrase and topoisomerase IV in vivo are considered desirable in requiring two target mutations for emergence of resistant bacteria. To investigate this...
Aubry, Alexandra, Pan, Xiao-Su, Fisher, L. Mark, Jarlier, Vincent, Cambau, Emmanuelle
Genome studies suggest that DNA gyrase is the sole type II topoisomerase and likely the unique target of quinolones in Mycobacterium tuberculosis. Despite the emerging importance of quinolones in the...
Ciprofloxacin Dimers Target Gyrase in Streptococcus pneumoniae
Gould, Katherine A., Pan, Xiao-Su, Kerns, Robert J., Fisher, L. Mark
We have examined the antipneumococcal activities of novel quinolone dimers in which ciprofloxacin was tethered to itself or to pipemidic acid by linkage of C-7 piperazinyl rings. Symmetric...
Streptococcus pneumoniae gyrA and gyrB genes specifying the DNA gyrase subunits have been cloned into pET plasmid vectors under the control of an inducible T7 promoter and have been separately...
Alovero, Fabiana L., Pan, Xiao-Su, Morris, Julia E., Manzo, Ruben H., Fisher, L. Mark
We have examined the antipneumococcal mechanisms of a series of novel fluoroquinolones that are identical to ciprofloxacin except for the addition of a benzenesulfonylamido group to the C-7...
Pan, Xiao-Su, Yague, Genoveva, Fisher, L. Mark
Mutations in DNA gyrase and/or topoisomerase IV genes are frequently encountered in quinolone-resistant mutants of Streptococcus pneumoniae. To investigate the mechanism of their effects at the...
DNA Gyrase and Topoisomerase IV Are Dual Targets of Clinafloxacin Action in Streptococcus pneumoniae
We examined the response of Streptococcus pneumoniae 7785 to clinafloxacin, a novel C-8-substituted fluoroquinolone which is being developed as an antipneumococcal agent. Clinafloxacin was highly...
Morris, Julia E., Pan, Xiao-Su, Fisher, L. Mark
Grepafloxacin, a 5-methyl-7-piperazinyl-3"-methyl analogue of ciprofloxacin, was used to obtain stepwise-selected mutants of Streptococcus pneumoniae 7785. Analysis of the quinolone...
Yague, Genoveva, Morris, Julia E., Pan, Xiao-Su, Gould, Katherine A., Fisher, L. Mark
Gemifloxacin is a recently developed fluoroquinolone with potent activity against Streptococcus pneumoniae. We show that the drug is more active than moxifloxacin, gatifloxacin, levofloxacin, and...
Pan, Xiao-Su, Hamlyn, Penelope J., Talens-Visconti, Raquel, Alovero, Fabiana L., Manzo, Ruben H., Fisher, L. Mark
Fluoroquinolones acting equally through DNA gyrase and topoisomerase IV in vivo are considered desirable in requiring two target mutations for emergence of resistant bacteria. To investigate this...
Aubry, Alexandra, Pan, Xiao-Su, Fisher, L. Mark, Jarlier, Vincent, Cambau, Emmanuelle
Genome studies suggest that DNA gyrase is the sole type II topoisomerase and likely the unique target of quinolones in Mycobacterium tuberculosis. Despite the emerging importance of quinolones in the...
Ciprofloxacin Dimers Target Gyrase in Streptococcus pneumoniae
Gould, Katherine A., Pan, Xiao-Su, Kerns, Robert J., Fisher, L. Mark
We have examined the antipneumococcal activities of novel quinolone dimers in which ciprofloxacin was tethered to itself or to pipemidic acid by linkage of C-7 piperazinyl rings. Symmetric...
Laponogov, Ivan, Veselkov, Dennis A., Sohi, Maninder K., Pan, Xiao-Su, Achari, Aniruddha, Yang, Cheng, ...
The 2.7 Å crystal structure of the 55-kDa N-terminal breakage-reunion domain of topoisomerase (topo) IV subunit A (ParC) from Streptococcus pneumoniae, the first for the quinolone targets from a...