Cruz-Flores, Mary Jane; Biology Department, College Of Science, De La Salle University-Manila, Manila, Philippines; Claveriaf@dlsu.edu.ph, Garcia Claveria, Florencia; Biology Department, College Of Science, De La Salle University-Manila, Manila, Philippines, Verdida, Rodolfo; Biology Department, College Of Science, De La Salle University-Manila, Manila, Philippines, Xuan, Xuenan; National Research Center For Protozoan Diseases, Obihiro University Of Agriculture And Veterinary Medicine, Hokkaido, Japan, Igarashi, Ikuo; National Research Center For Protozoan Diseases, Obihiro University Of Agriculture And Veterinary Medicine, Hokkaido, Japan
Istraživanje je provedeno kako bi se imunokromatografskim testom (P50t-ICT) dokazala prisutnost protozoona Babesia gibsoni u pasa lutalica na Filipinima. Ukupno je bilo pretraženo 46 pasa, 17...
Isolation and Identification of an Actin Gene From Babesia gibsoni (2006)
Jinlin Zhou, Bing Huang, Hiroshi Suzuki, Kozo Fujisaki, Ikuo Igarashi, Xuenan Xuan
Hiroaki Shirafuji, Xuenan Xuan, Isao Kimata, Yasuhiro Takashima, Shinya Fukumoto, Haruki Otsuka, ...
In this study, P23 of Cryptosporidium parvum sporozoites, an immunodominant surface protein, was stably expressed in Toxoplasma gondii (Tg/P23) and its protective effects were evaluated in a mouse...
Irungu Gaturaga, Bayin Chahan, Xuenan Xuan, Xiaohong Huang, Min Liao, Shinya Fukumoto, ...
The surface antigen 1–related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal...
Shinya Fukumoto, Yukiko Sekine, Xuenan Xuan, Ikuo Igarashi, Chihiro Sugimoto, Hideyuki Nagasawa, ...
The entire P50 gene encoding a surface protein of Babesia gibsoni was cloned into the bacteria expression vector pGEX-4T-3 and subsequently expressed in Escherichia coli as a glutathione...
Yasuhiro Takashima, Xuenan Xuan, Isao Kimata, Motohiro Iseki, Yoshikatsu Kodama, Noriko Nagane, ...
In order to develop a vaccine against cryptosporidiosis in cattle, we constructed a recombinant bovine herpesvirus-1 (BHV-1) expressing an immunodominant surface protein, p23, of Cryptosporidium...
Shinya Fukumoto, Xuenan Xuan, Ikuo Igarashi, Shoufa Zhang, Jennifer Mugisha, Takashi Ogata, ...
Canine red blood cell-substituted severe combined immune deficiency (Ca-RBC-SCID) mice were prepared for canine Babesia gibsoni infection. The Ca-RBC-SCID mice infected with B. gibsoni developed a...
Ikadai, Hiromi, Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Shigeyasu, Kanemaru, Takumi, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of B. caballi merozoite. A cDNA encoding a 48-kDa...
Xuan, Xuenan, Larsen, Alejandra, Ikadai, Hiromi, Tanaka, Tetsuya, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
The gene encoding the entire Babesia equi merozoite antigen 1 (EMA-1) was inserted into a baculovirus transfer vector, and a recombinant virus expressing EMA-1 was isolated. The expressed EMA-1 was...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Nishikawa, Yoshifumi, Kousaka, Yuko, Tragoolpua, Khajornsak, Xuan, Xuenan, Makala, Levi, Fujisaki, Kozo, ...
The baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. Here we have characterized the Neospora caninum surface protein NcSRS2 produced by two...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...
Boonchit, Suthisak, Xuan, Xuenan, Yokoyama, Naoaki, Goff, Will L., Wagner, Gale, Igarashi, Ikuo
The gene encoding Babesia bovis rhoptry-associated protein 1 (RAP-1) was used to develop an enzyme-linked immunosorbent assay (ELISA) to measure specific antibodies against B. bovis. The B. bovis...
Hirata, Haruyuki, Ikadai, Hiromi, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
To isolate Babesia equi genes encoding immunodominant proteins, a cDNA expression library prepared from B. equi mRNA was immunoscreened with B. equi-infected horse serum. Eighteen positive cDNA...
Yokoyama, Naoaki, Bork, Sabine, Nishisaka, Mitsuhiro, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, ...
Babesia microti, a hemoprotozoan parasite of rodents, is also important as a zoonotic agent of human babesiosis. The Maltese cross form, which consists of four masses in an erythrocyte, is...
Hirata, Haruyuki, Xuan, Xuenan, Yokoyama, Naoaki, Nishikawa, Yoshifumi, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported...
Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...
The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...
Nagai, Akiko, Yokoyama, Naoaki, Matsuo, Tomohide, Bork, Sabine, Hirata, Haruyuki, Xuan, Xuenan, ...
Three antimalarial drugs, artesunate, pyrimethamine, and pamaquine, were evaluated for their growth-inhibitory effects against Babesia equi and Babesia caballi in in vitro culture. B. equi was more...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Tamaki, Yoh, Hirata, Haruyuki, Takabatake, Noriyuki, Bork, Sabine, Yokoyama, Naoaki, Xuan, Xuenan, ...
A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could...
Huang, Xiaohong, Xuan, Xuenan, Xu, Longshan, Zhang, Shoufa, Yokoyama, Naoaki, Suzuki, Naoyoshi, ...
An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B. caballi-infected and...
Huang, Xiaohong, Xuan, Xuenan, Hirata, Haruyuki, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Naoyoshi, ...
An immunochromatographic test using recombinant truncated surface antigen 2 for detection of antibodies against Toxoplasma gondii was developed. Evaluation of detection of the antibody in mice and...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....
Boonchit, Suthisak, Xuan, Xuenan, Yokoyama, Naoaki, Goff, Will L., Waghela, Suryakant D., Wagner, Gale, ...
An enzyme-linked immunosorbent assay (ELISA) based on a recombinant rhoptry-associated protein-1 (RAP-1) of Babesia bovis has been previously developed, but it was imperfect because some...
Hirata, Haruyuki, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, Igarashi, Ikuo
In this study, we characterized a Babesia equi Be158 gene obtained by immunoscreening a B. equi cDNA expression phage library with B. equi-infected horse serum. The Be158 gene consists of an open...
Fukumoto, Shinya, Tamaki, Yoh, Shirafuji, Hiroaki, Harakawa, Shinji, Suzuki, Hiroshi, Xuan, Xuenan
This is a report of a vaccine trial directed against Babesia gibsoni infection in dogs with the use of the recombinant antigen P50. Dogs immunized with P50 showed partial protection manifested as a...
Liao, Min, Zhang, Shoufa, Xuan, Xuenan, Zhang, Guohong, Huang, Xiaohong, Igarashi, Ikuo, ...
An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to...
Huang, Xiaohong, Xuan, Xuenan, Verdida, Rodolfo A., Zhang, Shoufa, Yokoyama, Naoaki, Xu, Longshan, ...
An immunochromatographic test for the simultaneous detection of Babesia caballi- and B. equi-specific antibodies (BceICT) was developed using a recombinant B. caballi 48-kDa rhoptry protein (rBc48)...
Ikadai, Hiromi, Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Shigeyasu, Kanemaru, Takumi, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of B. caballi merozoite. A cDNA encoding a 48-kDa...
Xuan, Xuenan, Larsen, Alejandra, Ikadai, Hiromi, Tanaka, Tetsuya, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
The gene encoding the entire Babesia equi merozoite antigen 1 (EMA-1) was inserted into a baculovirus transfer vector, and a recombinant virus expressing EMA-1 was isolated. The expressed EMA-1 was...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Nishikawa, Yoshifumi, Kousaka, Yuko, Tragoolpua, Khajornsak, Xuan, Xuenan, Makala, Levi, Fujisaki, Kozo, ...
The baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. Here we have characterized the Neospora caninum surface protein NcSRS2 produced by two...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...
Boonchit, Suthisak, Xuan, Xuenan, Yokoyama, Naoaki, Goff, Will L., Wagner, Gale, Igarashi, Ikuo
The gene encoding Babesia bovis rhoptry-associated protein 1 (RAP-1) was used to develop an enzyme-linked immunosorbent assay (ELISA) to measure specific antibodies against B. bovis. The B. bovis...
Hirata, Haruyuki, Ikadai, Hiromi, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
To isolate Babesia equi genes encoding immunodominant proteins, a cDNA expression library prepared from B. equi mRNA was immunoscreened with B. equi-infected horse serum. Eighteen positive cDNA...
Yokoyama, Naoaki, Bork, Sabine, Nishisaka, Mitsuhiro, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, ...
Babesia microti, a hemoprotozoan parasite of rodents, is also important as a zoonotic agent of human babesiosis. The Maltese cross form, which consists of four masses in an erythrocyte, is...
Hirata, Haruyuki, Xuan, Xuenan, Yokoyama, Naoaki, Nishikawa, Yoshifumi, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported...
Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...
The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...
Nagai, Akiko, Yokoyama, Naoaki, Matsuo, Tomohide, Bork, Sabine, Hirata, Haruyuki, Xuan, Xuenan, ...
Three antimalarial drugs, artesunate, pyrimethamine, and pamaquine, were evaluated for their growth-inhibitory effects against Babesia equi and Babesia caballi in in vitro culture. B. equi was more...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Tamaki, Yoh, Hirata, Haruyuki, Takabatake, Noriyuki, Bork, Sabine, Yokoyama, Naoaki, Xuan, Xuenan, ...
A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could...
Huang, Xiaohong, Xuan, Xuenan, Xu, Longshan, Zhang, Shoufa, Yokoyama, Naoaki, Suzuki, Naoyoshi, ...
An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B. caballi-infected and...
Huang, Xiaohong, Xuan, Xuenan, Hirata, Haruyuki, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Naoyoshi, ...
An immunochromatographic test using recombinant truncated surface antigen 2 for detection of antibodies against Toxoplasma gondii was developed. Evaluation of detection of the antibody in mice and...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....
Boonchit, Suthisak, Xuan, Xuenan, Yokoyama, Naoaki, Goff, Will L., Waghela, Suryakant D., Wagner, Gale, ...
An enzyme-linked immunosorbent assay (ELISA) based on a recombinant rhoptry-associated protein-1 (RAP-1) of Babesia bovis has been previously developed, but it was imperfect because some...
Hirata, Haruyuki, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, Igarashi, Ikuo
In this study, we characterized a Babesia equi Be158 gene obtained by immunoscreening a B. equi cDNA expression phage library with B. equi-infected horse serum. The Be158 gene consists of an open...
Fukumoto, Shinya, Tamaki, Yoh, Shirafuji, Hiroaki, Harakawa, Shinji, Suzuki, Hiroshi, Xuan, Xuenan
This is a report of a vaccine trial directed against Babesia gibsoni infection in dogs with the use of the recombinant antigen P50. Dogs immunized with P50 showed partial protection manifested as a...
Liao, Min, Zhang, Shoufa, Xuan, Xuenan, Zhang, Guohong, Huang, Xiaohong, Igarashi, Ikuo, ...
An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to...
Huang, Xiaohong, Xuan, Xuenan, Verdida, Rodolfo A., Zhang, Shoufa, Yokoyama, Naoaki, Xu, Longshan, ...
An immunochromatographic test for the simultaneous detection of Babesia caballi- and B. equi-specific antibodies (BceICT) was developed using a recombinant B. caballi 48-kDa rhoptry protein (rBc48)...
Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii
Khan, Asis, Böhme, Ulrike, Kelly, Krystyna A., Adlem, Ellen, Brooks, Karen, Simmonds, Mark, ...
Toxoplasma gondii is a globally distributed protozoan parasite that can infect virtually all warm-blooded animals and humans. Despite the existence of a sexual phase in the life cycle, T. gondii has...
Babesial Vector Tick Defensin against Babesia sp. Parasites▿ †
Tsuji, Naotoshi, Battsetseg, Badgar, Boldbaatar, Damdinsuren, Miyoshi, Takeharu, Xuan, Xuenan, Oliver, James H., ...
Antimicrobial peptides are major components of host innate immunity, a well-conserved, evolutionarily ancient defensive mechanism. Infectious disease-bearing vector ticks are thought to possess...
Huang, Penglong, Liao, Min, Zhang, Houshuang, Lee, Eung-goo, Nishikawa, Yoshifumi, Xuan, Xuenan
To investigate whether the production of an antigen-specific antibody is associated with Neospora caninum-induced bovine abortion, 62 serum samples were tested with an enzyme-linked immunosorbent...
A Cysteine Protease Is Critical for Babesia spp. Transmission in Haemaphysalis Ticks
Tsuji, Naotoshi, Miyoshi, Takeharu, Battsetseg, Badger, Matsuo, Tomohide, Xuan, Xuenan, Fujisaki, Kozo
Vector ticks possess a unique system that enables them to digest large amounts of host blood and to transmit various animal and human pathogens, suggesting the existence of evolutionally acquired...