Y. C. Awasthi

Interrelationship of hexosaminidases A and B: conformation of the common and the unique subunit theory.

Srivastava, S K, Wiktorowicz, J E, Awasthi, Y C

Human kidney hexosaminidase A (beta-N-acetylglucosaminidase; 2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase; EC 3.2.1.30) is a heteropolymer of two immunologically distinct...

Nomenclature for human glutathione transferases.

Mannervik, B, Awasthi, Y C, Board, P G, Hayes, J D, Di Ilio, C, Ketterer, B, ...

Human glutathione S-transferases. Characterization of the anionic forms from lung and placenta.

Dao, D D, Partridge, C A, Kurosky, A, Awasthi, Y C

Anionic glutathione S-transferases were purified from human lung and placenta. Chemical and immunochemical characterization, including polyacrylamide-gel electrophoresis, gave strong evidence that...

Rat lung glutathione S-transferases. Evidence for two distinct types of 22000-Mr subunits.

Singh, S V, Partridge, C A, Awasthi, Y C

Two immunologically distinct types of 22000-Mr subunits are present in rat lung glutathione S-transferases. One of these subunits is probably similar to Ya subunits of rat liver glutathione...

Two immunologically distinct Yc-type subunits are present in rat lung glutathione S-transferases.

Singh, S V, Awasthi, Y C

Two types of 25 000-Mr subunits are present in rat lung glutathione S-transferase I (pI 8.8). These subunits, designated Yc and Yc', are immunologically and functionally distinct from each other. The...

Glutathione S-transferases of human brain. Evidence for two immunologically distinct types of 26500-Mr subunits.

Theodore, C, Singh, S V, Hong, T D, Awasthi, Y C

Human brain contains one cationic (pI8.3) and two anionic (pI5.5 and 4.6) forms of glutathione S-transferase. The cationic form (pI8.3) and the less-anionic form (pI5.5) do not correspond to any of...

Interactions with glutathione S-transferases of porphyrins used in photodynamic therapy and naturally occurring porphyrins.

Smith, A, Nuiry, I, Awasthi, Y C

Several naturally occurring porphyrins and porphyrins used in photodynamic therapy inhibit glutathione S-transferase isoenzymes either purified from rat liver or lung or in cytosol from normal and...

Dinitrophenyl glutathione efflux from human erythrocytes is primary active ATP-dependent transport.

LaBelle, E F, Singh, S V, Srivastava, S K, Awasthi, Y C

Dinitrophenyl S-glutathione is accumulated by inside-out vesicles made from human erythrocytes in a process totally dependent on ATP and Mg2+. The vesicles were shown to accumulate dinitrophenyl...

Human liver glutathione S-transferase psi. Chemical characterization and secondary-structure comparison with other mammalian glutathione S-transferases.

Singh, S V, Kurosky, A, Awasthi, Y C

The isolation and chemical characterization of the anionic human liver glutathione S-transferase (GST) psi (pI 5.5) are described and compared with other GST isoenzymes reported for rat and human....

Glutathione S-transferases of mouse lung. Selective binding of benzo[a]pyrene metabolites by the subunits which are preferentially induced by t-butylated hydroxyanisole.

Singh, S V, Creadon, G, Das, M, Mukhtar, H, Awasthi, Y C

Six isoenzymes of glutathione S-transferase (GST) present in mouse lung have been purified and characterized. GST I (pI 9.8) is a dimer of Mr-26,500 subunits and GST II is a heterodimer of Mr-26,500...

Purification and characterization of glutathione S-transferases of human kidney.

Singh, S V, Leal, T, Ansari, G A, Awasthi, Y C

Several forms of glutathione S-transferase (GST) are present in human kidney, and the overall isoenzyme pattern of kidney differs significantly from those of other human tissues. All the three major...

Independent segregation of glutathione S-transferase and fatty acid ethyl ester synthase from pancreas and other human tissues.

Sharma, R, Gupta, S, Singhal, S S, Ahmad, H, Haque, A, Awasthi, Y C

Glutathione S-transferase (GST) isoenzymes of human pancreas were purified, characterized and evaluated for their possible role in the metabolism of ethanol. Human pancreas has at least two GST...

Different forms of human liver glutathione S-transferases arise from dimeric combinations of at least four immunologically and functionally distinct subunits.

Singh, S V, Dao, D D, Partridge, C A, Theodore, C, Srivastava, S K, Awasthi, Y C

Four immunologically distinct subunits were characterized in glutathione (GSH) S-transferases of human liver. Five cationic enzymes (pI 8.9, 8.5, 8.3, 8.2 and 8.0) have an apparently similar subunit...

Glutathione S-transferases of the bovine retina. Evidence that glutathione peroxidase activity is the result of glutathione S-transferase.

Saneto, R P, Awasthi, Y C, Srivastava, S K

We have purified two isoenzymes of glutathione S-transferase from bovine retina to apparent homogeneity through a combination of gel-filtration chromatography, affinity chromatography and isoelectric...

Interrelationship between anionic and cationic forms of glutathione S-transferases of human liver.

Awasthi, Y C, Dao, D D, Saneto, R P

Human liver glutathione S-transferases (GSH S-transferases) were fractionated into cationic and anionic proteins. During fractionation with (NH4)2SO4 the anionic GSH S-transferases are concentrated...

Interrelationship between cationic and anionic forms of glutathione S-transferases of bovine ocular lens.

Saneto, R P, Awasthi, Y C, Srivastava, S K

Since the eye is constantly exposed to potentially damaging chemical compounds present in the atmosphere and vascular system, we investigated the physiological role of glutathione S-transferase (GSH...

Purification and properties of human kidney-cortex hexosaminidases A and B.

Wiktorowicz, J E, Awasthi, Y C, Kurosky, A, Srivastava, S K

Hexosaminidases (EC 3.2.1.30) A and B from human kidney cortex were purified to homogeneity by using concanavalin A affinity chromatography, ion-exchange chromatography and gel filtration. The yield...

Purification and properties of glutathione peroxidase from human placenta.

Awasthi, Y C, Dao, D D, Lal, A K, Srivastava, S K

Glutathione peroxidase (glutathione--H2O2 oxidoreductase; EC 1.11.1.9) was purified to homogeneity from human placenta by using (NH4)2SO4 precipitation, ion-exchange chromatography, Sephadex gel...

Characterization of a novel glutathione S-transferase isoenzyme from mouse lung and liver having structural similarity to rat glutathione S-transferase 8-8.

Medh, R D, Saxena, M, Singhal, S S, Ahmad, H, Awasthi, Y C

In mouse lung, glutathione S-transferase (GST, EC 2.5.1.18) isoenzymes belonging to the three major known classes, Alpha, Mu and Pi, have been previously characterized, along with an isoenzyme (pI...

Interrelationship of hexosaminidases A and B: conformation of the common and the unique subunit theory.

Srivastava, S K, Wiktorowicz, J E, Awasthi, Y C

Human kidney hexosaminidase A (beta-N-acetylglucosaminidase; 2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase; EC 3.2.1.30) is a heteropolymer of two immunologically distinct...

Nomenclature for human glutathione transferases.

Mannervik, B, Awasthi, Y C, Board, P G, Hayes, J D, Di Ilio, C, Ketterer, B, ...

Human glutathione S-transferases. Characterization of the anionic forms from lung and placenta.

Dao, D D, Partridge, C A, Kurosky, A, Awasthi, Y C

Anionic glutathione S-transferases were purified from human lung and placenta. Chemical and immunochemical characterization, including polyacrylamide-gel electrophoresis, gave strong evidence that...

Rat lung glutathione S-transferases. Evidence for two distinct types of 22000-Mr subunits.

Singh, S V, Partridge, C A, Awasthi, Y C

Two immunologically distinct types of 22000-Mr subunits are present in rat lung glutathione S-transferases. One of these subunits is probably similar to Ya subunits of rat liver glutathione...

Two immunologically distinct Yc-type subunits are present in rat lung glutathione S-transferases.

Singh, S V, Awasthi, Y C

Two types of 25 000-Mr subunits are present in rat lung glutathione S-transferase I (pI 8.8). These subunits, designated Yc and Yc', are immunologically and functionally distinct from each other. The...

Glutathione S-transferases of human brain. Evidence for two immunologically distinct types of 26500-Mr subunits.

Theodore, C, Singh, S V, Hong, T D, Awasthi, Y C

Human brain contains one cationic (pI8.3) and two anionic (pI5.5 and 4.6) forms of glutathione S-transferase. The cationic form (pI8.3) and the less-anionic form (pI5.5) do not correspond to any of...

Interactions with glutathione S-transferases of porphyrins used in photodynamic therapy and naturally occurring porphyrins.

Smith, A, Nuiry, I, Awasthi, Y C

Several naturally occurring porphyrins and porphyrins used in photodynamic therapy inhibit glutathione S-transferase isoenzymes either purified from rat liver or lung or in cytosol from normal and...

Dinitrophenyl glutathione efflux from human erythrocytes is primary active ATP-dependent transport.

LaBelle, E F, Singh, S V, Srivastava, S K, Awasthi, Y C

Dinitrophenyl S-glutathione is accumulated by inside-out vesicles made from human erythrocytes in a process totally dependent on ATP and Mg2+. The vesicles were shown to accumulate dinitrophenyl...

Human liver glutathione S-transferase psi. Chemical characterization and secondary-structure comparison with other mammalian glutathione S-transferases.

Singh, S V, Kurosky, A, Awasthi, Y C

The isolation and chemical characterization of the anionic human liver glutathione S-transferase (GST) psi (pI 5.5) are described and compared with other GST isoenzymes reported for rat and human....

Glutathione S-transferases of mouse lung. Selective binding of benzo[a]pyrene metabolites by the subunits which are preferentially induced by t-butylated hydroxyanisole.

Singh, S V, Creadon, G, Das, M, Mukhtar, H, Awasthi, Y C

Six isoenzymes of glutathione S-transferase (GST) present in mouse lung have been purified and characterized. GST I (pI 9.8) is a dimer of Mr-26,500 subunits and GST II is a heterodimer of Mr-26,500...

Purification and characterization of glutathione S-transferases of human kidney.

Singh, S V, Leal, T, Ansari, G A, Awasthi, Y C

Several forms of glutathione S-transferase (GST) are present in human kidney, and the overall isoenzyme pattern of kidney differs significantly from those of other human tissues. All the three major...

Independent segregation of glutathione S-transferase and fatty acid ethyl ester synthase from pancreas and other human tissues.

Sharma, R, Gupta, S, Singhal, S S, Ahmad, H, Haque, A, Awasthi, Y C

Glutathione S-transferase (GST) isoenzymes of human pancreas were purified, characterized and evaluated for their possible role in the metabolism of ethanol. Human pancreas has at least two GST...

Characterization of a novel glutathione S-transferase isoenzyme from mouse lung and liver having structural similarity to rat glutathione S-transferase 8-8.

Medh, R D, Saxena, M, Singhal, S S, Ahmad, H, Awasthi, Y C

In mouse lung, glutathione S-transferase (GST, EC 2.5.1.18) isoenzymes belonging to the three major known classes, Alpha, Mu and Pi, have been previously characterized, along with an isoenzyme (pI...

Different forms of human liver glutathione S-transferases arise from dimeric combinations of at least four immunologically and functionally distinct subunits.

Singh, S V, Dao, D D, Partridge, C A, Theodore, C, Srivastava, S K, Awasthi, Y C

Four immunologically distinct subunits were characterized in glutathione (GSH) S-transferases of human liver. Five cationic enzymes (pI 8.9, 8.5, 8.3, 8.2 and 8.0) have an apparently similar subunit...

Glutathione S-transferases of the bovine retina. Evidence that glutathione peroxidase activity is the result of glutathione S-transferase.

Saneto, R P, Awasthi, Y C, Srivastava, S K

We have purified two isoenzymes of glutathione S-transferase from bovine retina to apparent homogeneity through a combination of gel-filtration chromatography, affinity chromatography and isoelectric...

Interrelationship between anionic and cationic forms of glutathione S-transferases of human liver.

Awasthi, Y C, Dao, D D, Saneto, R P

Human liver glutathione S-transferases (GSH S-transferases) were fractionated into cationic and anionic proteins. During fractionation with (NH4)2SO4 the anionic GSH S-transferases are concentrated...

Interrelationship between cationic and anionic forms of glutathione S-transferases of bovine ocular lens.

Saneto, R P, Awasthi, Y C, Srivastava, S K

Since the eye is constantly exposed to potentially damaging chemical compounds present in the atmosphere and vascular system, we investigated the physiological role of glutathione S-transferase (GSH...

Purification and properties of human kidney-cortex hexosaminidases A and B.

Wiktorowicz, J E, Awasthi, Y C, Kurosky, A, Srivastava, S K

Hexosaminidases (EC 3.2.1.30) A and B from human kidney cortex were purified to homogeneity by using concanavalin A affinity chromatography, ion-exchange chromatography and gel filtration. The yield...

Purification and properties of glutathione peroxidase from human placenta.

Awasthi, Y C, Dao, D D, Lal, A K, Srivastava, S K

Glutathione peroxidase (glutathione--H2O2 oxidoreductase; EC 1.11.1.9) was purified to homogeneity from human placenta by using (NH4)2SO4 precipitation, ion-exchange chromatography, Sephadex gel...

Increased oxidative DNA damage and hepatocyte overexpression of specific cytochrome P450 isoforms in hepatitis of mice infected with Helicobacter hepaticus.

Sipowicz, M. A., Chomarat, P., Diwan, B. A., Anver, M. A., Awasthi, Y. C., Ward, J. M., ...

A recently discovered bacterium, Helicobacter hepaticus, infects the intrahepatic bile canaliculi of mice, causing a severe chronic hepatitis culminating in liver cancer. Thus, it affords an animal...