Scientific Commons: Y. Fujii-Kuriyama

Possible function of Ah receptor nuclear translocator (Arnt) homodimer in transcriptional regulation.

Sogawa, K, Nakano, R, Kobayashi, A, Kikuchi, Y, Ohe, N, Matsushita, N, ...

Arnt (Ah receptor nuclear translocator) is a member of a transcription factor family having characteristic motifs designated bHLH (basic helix-loop-helix) and PAS and was originally found as a factor...

cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt).

Hirose, K, Morita, M, Ema, M, Mimura, J, Hamada, H, Fujii, H, ...

We isolated mouse cDNA clones (Arnt2) that are highly similar to but distinct from the aryl hydrocarbon receptor (AhR) nuclear translocator (Arnt). The composite cDNA covered a 2,443-bp sequence...

Two new members of the murine Sim gene family are transcriptional repressors and show different expression patterns during mouse embryogenesis.

Ema, M, Morita, M, Ikawa, S, Tanaka, M, Matsuda, Y, Gotoh, O, ...

From a cDNA library of mouse skeletal muscle, we have isolated mouse Sim1 (mSim1) cDNA encoding a polypeptide of 765 amino acids with striking amino acid identify in basic helix-loop-helix (89%...

Purification and properties of beta-lactamase from Proteus morganii.

Fujii-Kuriyama, Y, Yamamoto, M, Sugawara, S

The cephalosporin beta-lactamase was purified from a strain of Proteus morganii that showed resistance to beta-lactam antibiotics and produced the enzyme constitutively. The purified enzyme...

A DNA-binding factor specific for xenobiotic responsive elements of P-450c gene exists as a cryptic form in cytoplasm: its possible translocation to nucleus.

Fujisawa-Sehara, A, Yamane, M, Fujii-Kuriyama, Y

Transcription of the drug-metabolizing cytochrome P-450c gene is induced by 3-methylcholanthrene or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Previously, we defined two xenobiotic responsive...

Aberrant splicing and missense mutations cause steroid 21-hydroxylase [P-450(C21)] deficiency in humans: possible gene conversion products.

Higashi, Y, Tanae, A, Inoue, H, Hiromasa, T, Fujii-Kuriyama, Y

Four steroid 21-hydroxylase B [P-450(C21)B] genes (designated P.7, P.10-1, P.10-2, and P.3) from three P-450(C21)-deficient patients were isolated to analyze their structures and functions. Several...

Molecular analysis of patient and carrier genes with congenital steroid 21-hydroxylase deficiency by using polymerase chain reaction and single strand conformation polymorphism.

Tajima, T, Fujieda, K, Nakayama, K, Fujii-Kuriyama, Y

Steroid 21-hydroxylase deficiency is a major cause of congenital adrenal hyperplasia and is caused by genetic impairment of this enzyme. Since approximately 80% of cases are caused by point mutations...

cDNA cloning and transcriptional properties of a novel GC box-binding protein, BTEB2.

Sogawa, K, Imataka, H, Yamasaki, Y, Kusume, H, Abe, H, Fujii-Kuriyama, Y

We have cloned a cDNA for a novel GC box-binding protein designated BTEB2 from a human placenta cDNA library using rat BTEB cDNA (Imataka et al. (1992). EMBO J. 11,3663-3671. as a hybridization...

Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs.

Nabeshima, Y, Fujii-Kuriyama, Y, Muramatsu, M, Ogata, K

We report here the molecular cloning and sequence analysis of DNAs complementary to mRNAs for myosin alkali light chain of chicken embryo and adult leg skeletal muscle. pSMA2-1 contained an 818...

Complete nucleotide sequence of two steroid 21-hydroxylase genes tandemly arranged in human chromosome: a pseudogene and a genuine gene.

Higashi, Y, Yoshioka, H, Yamane, M, Gotoh, O, Fujii-Kuriyama, Y

Two 21-hydroxylase [P-450(C21)] genes have been isolated from a human genomic library using a bovine P-450(C21) cDNA. The insert DNAs containing the P-450(C21) genes were also hybridized with the...

Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene.

Fujisawa-Sehara, A, Sogawa, K, Nishi, C, Fujii-Kuriyama, Y

We have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. After combining the 5' flanking and upstream untranslated regions of the isolated P-450c...

Characterization of xenobiotic responsive elements upstream from the drug-metabolizing cytochrome P-450c gene: a similarity to glucocorticoid regulatory elements.

Fujisawa-Sehara, A, Sogawa, K, Yamane, M, Fujii-Kuriyama, Y

The DNA element governing the inducible expression of drug-metabolizing P-450c gene by xenobiotic treatments was investigated by gene transfer methods. A variety of dissected fragments from -844 to...

Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver.

Kawajiri, K, Gotoh, O, Sogawa, K, Tagashira, Y, Muramatsu, M, Fujii-Kuriyama, Y

We determined the coding nucleotide sequence of the mRNA for a 3-methylcholanthrene-inducible cytochrome P-450, P-450d, of rat liver by sequence analysis of cloned cDNAs. The predicted amino acid...

Primary structure of a cytochrome P-450: coding nucleotide sequence of phenobarbital-inducible cytochrome P-450 cDNA from rat liver.

Fujii-Kuriyama, Y, Mizukami, Y, Kawajiri, K, Sogawa, K, Muramatsu, M

We determined the coding nucleotide sequence of phenobarbital-inducible cytochrome P-450 mRNA of the rat by analysis of three cloned cDNAs and by primer extension methods. The deduced amino acid...

Molecular cloning of human interferon cDNA.

Taniguchi, T, Fujii-Kuriyama, Y, Muramatsu, M

A hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S. & Sudo, T. (1979)...

A novel cis-acting DNA element required for a high level of inducible expression of the rat P-450c gene.

Yanagida, A, Sogawa, K, Yasumoto, K I, Fujii-Kuriyama, Y

A novel cis-acting regulatory element (designated BTE for basic transcription element) was found in the region proximal to the TATA sequence of the P-450c gene by the use of deletion mutations. This...

Upstream regulatory region for inducible expression of the chicken skeletal myosin alkali light-chain gene.

Shirakata, M, Nabeshima, Y, Konishi, K, Fujii-Kuriyama, Y

The expression of the fast type of myosin alkali light chain 1 is induced during the differentiation of muscle cells. To study the mechanism of its gene regulation, we joined the sequence of the...

Mechanism of action of a repressor of dioxin-dependent induction of Cyp1a1 gene transcription.

Watson, A J, Weir-Brown, K I, Bannister, R M, Chu, F F, Reisz-Porszasz, S, Fujii-Kuriyama, Y, ...

A dominant mutant of Hepa-1 cells, c31, expresses a repressor that prevents 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-dependent stimulation of Cyp1a1 transcription. The repressor acts via the...

Location of regulatory elements responsible for drug induction in the rat cytochrome P-450c gene.

Sogawa, K, Fujisawa-Sehara, A, Yamane, M, Fujii-Kuriyama, Y

The synthesis of cytochrome P-450c is induced remarkably in cultured cells as well as animal tissues in response to added chemicals such as 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzodioxin....

Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex.

Morohashi, K, Fujii-Kuriyama, Y, Okada, Y, Sogawa, K, Hirose, T, Inayama, S, ...

We have isolated cDNA clones of the mRNA for cytochrome P-450(SCC), which catalyzes the side-chain cleavage reaction of cholesterol in bovine adrenal cortex mitochondria, by using synthetic...

Distinct organization of methylcholanthrene- and phenobarbital-inducible cytochrome P-450 genes in the rat.

Sogawa, K, Gotoh, O, Kawajiri, K, Fujii-Kuriyama, Y

The complete nucleotide sequence of the methylcholanthrene-inducible cytochrome P-450c gene was determined by sequence analysis of cloned genomic DNA and the sequence, consisting of 524 amino acids,...

Gene structure of a phenobarbital-inducible cytochrome P-450 in rat liver.

Mizukami, Y, Sogawa, K, Suwa, Y, Muramatsu, M, Fujii-Kuriyama, Y

The gene structure of a phenobarbital-inducible rat liver cytochrome P-50 was elucidated by sequence analysis of two isolated genomic clones. The total length of the gene was approximately 14...

Two regulatory proteins that bind to the basic transcription element (BTE), a GC box sequence in the promoter region of the rat P-4501A1 gene.

Imataka, H, Sogawa, K, Yasumoto, K, Kikuchi, Y, Sasano, K, Kobayashi, A, ...

The cDNAs for two DNA binding proteins of BTE, a GC box sequence in the promoter region of the P-450IA1(CYP1A1) gene, have been isolated from a rat liver cDNA library by using the BTE sequence as a...

Metabolic inactivation of retinoic acid by a novel P450 differentially expressed in developing mouse embryos.

Fujii, H, Sato, T, Kaneko, S, Gotoh, O, Fujii-Kuriyama, Y, Osawa, K, ...

Retinoic acid (RA) is a physiological agent that has a wide range of biological activity and appears to regulate developmental programs of vertebrates. However, little is known about the molecular...

Molecular mechanisms of transcription activation by HLF and HIF1alpha in response to hypoxia: their stabilization and redox signal-induced interaction with CBP/p300.

Ema, M, Hirota, K, Mimura, J, Abe, H, Yodoi, J, Sogawa, K, ...

Hypoxia-inducible factor 1 alpha (HIF1alpha) and its related factor, HLF, activate expression of a group of genes such as erythropoietin in response to low oxygen. Transfection analysis using fusion...