Baum, E Z, Bebernitz, G A, Gluzman, Y
The protease encoded by the pol gene of human immunodeficiency virus was expressed in Escherichia coli and found to be toxic to strain BL21(DE3). This toxicity provided a convenient selection for...
Baum, E Z, Bebernitz, G A, Gluzman, Y
A "cleavage cassette" specifying a decapeptide human immunodeficiency virus (HIV) protease cleavage site was introduced into six different locations of beta-galactosidase (beta-D-galactoside...
Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636.
Rasmussen, B A, Gluzman, Y, Tally, F P
Bacteroides fragilis TAL3636 produces a class B, Zn2(+)-requiring beta-lactamase. The gene, ccrA, was cloned and expressed in Escherichia coli. The gene was sequenced and shown to share greater than...
Morin, J, Johann, S, O'Hara, B, Gluzman, Y
The effect of human cytomegalovirus infection on cellular DNA synthesis in human fibroblasts was measured by fluorometry and by incorporation of radiolabeled thymidine. The results show that although...
He, B, Chou, J, Brandimarti, R, Mohr, I, Gluzman, Y, Roizman, B
Earlier studies have shown that infection of human cells by herpes simplex virus 1 (HSV-1) results in the activation of RNA-dependent protein kinase (PKR) but that the alpha subunit of eIF-2 is not...
Baum, E Z, Bebernitz, G A, Hulmes, J D, Muzithras, V P, Jones, T R, Gluzman, Y
The 45-kDa assembly protein of human cytomegalovirus is encoded by the C-terminal portion of the UL80 open reading frame (ORF). For herpes simplex virus, packaging of DNA is accompanied by cleavage...
McVey, D, Ray, S, Gluzman, Y, Berger, L, Wildeman, A G, Marshak, D R, ...
Phosphorylation of simian virus 40 (SV40) T antigen on threonine 124 activates viral DNA replication in vivo and in vitro. We have manipulated the modification of T-antigen residue 124 both...
Rasmussen, B, Noller, H F, Daubresse, G, Oliva, B, Misulovin, Z, Rothstein, D M, ...
Tetracycline analogs fell into two classes on the basis of their mode of action. Tetracycline, chlortetracycline, minocycline, doxycycline, and 6-demethyl-6-deoxytetracycline inhibited cell-free...
Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli.
Plotch, S J, Palant, O, Gluzman, Y
A cDNA clone encoding the RNA polymerase of poliovirus has been expressed in Escherichia coli under the transcriptional control of a T7 bacteriophage promoter. The poliovirus enzyme was designed to...
Jones, T R, Muzithras, V P, Gluzman, Y
The US6 gene family, located within the unique short region (US) of the human cytomegalovirus (HCMV) genome, contains six open reading frames (US6 through US11) which may encode glycoproteins, such...
Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
Mohr, I J, Fairman, M P, Stillman, B, Gluzman, Y
The biochemical activities of a series of transformation-competent, replication-defective large T-antigen point mutants were examined. The assays employed reflect partial reactions required for the...
Analysis of nonpermissivity in mouse cells overexpressing simian virus 40 T antigen.
Gerard, R D, Guggenheimer, R A, Gluzman, Y
To analyze the nature of the nonpermissivity of mouse cells for simian virus 40 (SV40) DNA replication, we isolated mouse cells producing SV40 T antigen (Tag) at levels equal to or greater than that...
To study the role of the biochemical and physiological activities of simian virus 40 (SV40) large T antigen in the lytic and transformation processes, we have analyzed DNA replication-defective,...
Van Doren, K, Hanahan, D, Gluzman, Y
Recombinant viral genomes carrying a selectable drug resistance marker have been constructed by insertion of a hybrid gene for neomycin resistance into the helper-independent adenovirus vector, delta...
Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
Strauss, M, Argani, P, Mohr, I J, Gluzman, Y
The origin-specific DNA-binding domain of simian virus 40 large T antigen was analyzed, and its C-terminal boundary was found to be at or before amino acid 259. This does not include the zinc finger...
Palindromic adenovirus type 5-simian virus 40 hybrid.
A family of novel adenovirus type 5-simian virus 40 (Ad5-SV40) recombinants (Ad5++D1) whose genomes consist of symmetrically inverted structures was isolated. Particles of Ad5++D1 could contain one...
Rasmussen, B A, Gluzman, Y, Tally, F P
One of the two major mechanisms of tetracycline resistance is ribosomal protection. Of this resistance type, tet(M) is the best characterized. Although the mechanism of tet(M) resistance has not yet...
Maulbecker, C, Mohr, I, Gluzman, Y, Bartholomew, J, Botchan, M
We describe a new complementation function within the simian virus 40 (SV40) A gene. This function is required for viral DNA replication and virus production in vivo but, surprisingly, does not...
Mohr, I J, Gluzman, Y, Fairman, M P, Strauss, M, McVey, D, Stillman, B, ...
A bacterial expression system was used to produce simian virus 40 large tumor antigen (T antigen) in the absence of the extensive posttranslational modifications that occur in mammalian cells....
Ghosh, P K, Lebowitz, P, Frisque, R J, Gluzman, Y
The 5' termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template. The 5'...
Sequence of the junction in adenovirus 2-SV40 hybrids: examples of illegitimate recombination.
Ling, L E, Manos, M M, Gluzman, Y
The nucleotide sequences of six Ad2-SV40 junctions from three Ad2-SV40 hybrid viruses (Ad2++HEY, Ad2++LEY and Ad2+D1) were determined. Comparison of parental adenovirus 2 and SV40 DNA sequences with...
Expression of early genes of origin-defective mutants of simian virus 40.
Gluzman, Y, Sambrook, J F, Frisque, R J
The nucleotide sequences of eight origin-defective mutants of simian virus 40 have been determined. All of the mutants have suffered deletions, which range in size from 4 to 241 nucleotides. Some of...
Altered restriction endonuclease cleavage pattern of simian virus 40 DNA.
Three different groups of temperature-sensitive mutants of simian virus 40, isolated and characterized by Chou and Martin (J. Virol. 13:1101--1109, 1974), have been analyzed by using restriction...
Cellular and cell-free synthesis of simian virus 40 T-antigens in permissive and transformed cells.
Prives, C, Gluzman, Y, Winocour, E
mRNA extracted from a variety of simian virus 40 (SV40)-infected monkey cell lines directs the cell-free synthesis of viral T-antigen polypeptides with molecular weights estimated as 90,000 and...
Properties of the DNA-binding domain of the simian virus 40 large T antigen.
McVey, D, Strauss, M, Gluzman, Y
T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these...
Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
Soluble extracts prepared from the nucleus and cytoplasm of human 293 cells are capable of efficient replication and supercoiling of added DNA templates that contain the origin of simian virus 40...
One boundary of the minimal origin of replication of simian virus 40 DNA lies within the A + T-rich region. Deletion of only a few bases into the adenine-thymine (AT) stretch results in a DNA...
Construction of a helper-free recombinant adenovirus that expresses polyomavirus large T antigen.
Massie, B, Gluzman, Y, Hassell, J A
Adenovirus-polyomavirus recombinant viruses were constructed in vitro by inserting a hybrid transcription unit composed of the adenovirus type 2 major late promoter and the early coding region of...
A variant of the adenovirus type 5 genome which lacks EcoRI sites has been cloned in a bacterial plasmid after the addition of EcoRI oligonucleotide linkers to its ends. Closed circular forms of the...
The large T antigen of simian virus 40 (SV40) is a multifunctional protein that is essential in both the virus lytic cycle and the oncogenic transformation of cells by SV40. To investigate the role...
Efficient transformation of human fibroblasts by adenovirus-simian virus 40 recombinants.
The origin-defective simian virus 40 (SV40) mutant 6-1 has been useful in transforming human cells (Small et al., Nature [London] 296:671-672, 1982; Nagata et al., Nature [London] 306:597-599, 1983)....
New host cell system for regulated simian virus 40 DNA replication.
Transformed monkey cell lines (CMT and BMT) that inducible express simian virus 40 (SV40) T antigen from the metallothionein promoter have been isolated and characterized. Immunoprecipitation of...
DNA-binding properties of simian virus 40 T-antigen mutants defective in viral DNA replication.
Prives, C, Covey, L, Scheller, A, Gluzman, Y
Three simian virus 40 (SV40)-transformed monkey cell lines, C2, C6, and C11, producing T-antigen variants that are unable to initiate viral DNA replication, were analyzed with respect to their...
Transfer of genes into hematopoietic cells using recombinant DNA viruses.
Karlsson, S, Humphries, R K, Gluzman, Y, Nienhuis, A W
The ability of recombinant DNA viruses to transfer genes into hematopoietic cells has been explored. A recombinant simian virus 40 (SV40) in which the early region had been replaced with the...
A herpesvirus genetic element which affects translation in the absence of the viral GADD34 function.
Novel suppressor variants of conditionally lethal HSV-1 gamma34.5 deletion mutants have been isolated which exhibit restored ability to grow on neoplastic neuronal cells. Deletion of the viral...
Properties of permissive monkey cells transformed by UV-irradiated simian virus 40.
Gluzman, Y, Davison, J, Oren, M, Winocour, E
African green monkey cells (CV1 line) were infected with UV-irradiated simian virus 40 (SV40), and permissive lines of stably transformed cells were established. These cell lines display the SV40...
Gluzman, Y., Kuff, E. L., Winocour, E.
Passage of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 at the permissive temperature in each of three permissive lines of SV40-transformed monkey CV1 cells resulted in the...
Vogel, T., Gluzman, Y., Winocour, E.
The genome of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol....
Regulation of SV40 DNA replication by phosphorylation of T antigen.
Mohr, I J, Stillman, B, Gluzman, Y
The role of phosphorylation in regulating the biochemical properties of SV40 large T antigen has been examined. Treatment of purified T antigen with calf intestinal alkaline phosphatase resulted in...
T antigen and template requirements for SV40 DNA replication in vitro.
Stillman, B, Gerard, R D, Guggenheimer, R A, Gluzman, Y
A cell-free system for replication of SV40 DNA was used to assess the effect of mutations altering either the SV40 origin of DNA replication or the virus-encoded large tumor (T) antigen. Plasmid DNAs...
Stable gene transfer and tissue-specific expression of a human globin gene using adenoviral vectors.
Karlsson, S, Van Doren, K, Schweiger, S G, Nienhuis, A W, Gluzman, Y
Helper-free double recombinant adenoviruses containing a genomic human globin gene and the neomycin resistance gene (neoR) have been constructed. The inserted globin and neoR genes are stable and...
Baum, E Z, Bebernitz, G A, Gluzman, Y
The protease encoded by the pol gene of human immunodeficiency virus was expressed in Escherichia coli and found to be toxic to strain BL21(DE3). This toxicity provided a convenient selection for...
Baum, E Z, Bebernitz, G A, Gluzman, Y
A "cleavage cassette" specifying a decapeptide human immunodeficiency virus (HIV) protease cleavage site was introduced into six different locations of beta-galactosidase (beta-D-galactoside...
Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636.
Rasmussen, B A, Gluzman, Y, Tally, F P
Bacteroides fragilis TAL3636 produces a class B, Zn2(+)-requiring beta-lactamase. The gene, ccrA, was cloned and expressed in Escherichia coli. The gene was sequenced and shown to share greater than...
Morin, J, Johann, S, O'Hara, B, Gluzman, Y
The effect of human cytomegalovirus infection on cellular DNA synthesis in human fibroblasts was measured by fluorometry and by incorporation of radiolabeled thymidine. The results show that although...
He, B, Chou, J, Brandimarti, R, Mohr, I, Gluzman, Y, Roizman, B
Earlier studies have shown that infection of human cells by herpes simplex virus 1 (HSV-1) results in the activation of RNA-dependent protein kinase (PKR) but that the alpha subunit of eIF-2 is not...
Baum, E Z, Bebernitz, G A, Hulmes, J D, Muzithras, V P, Jones, T R, Gluzman, Y
The 45-kDa assembly protein of human cytomegalovirus is encoded by the C-terminal portion of the UL80 open reading frame (ORF). For herpes simplex virus, packaging of DNA is accompanied by cleavage...
McVey, D, Ray, S, Gluzman, Y, Berger, L, Wildeman, A G, Marshak, D R, ...
Phosphorylation of simian virus 40 (SV40) T antigen on threonine 124 activates viral DNA replication in vivo and in vitro. We have manipulated the modification of T-antigen residue 124 both...
Rasmussen, B, Noller, H F, Daubresse, G, Oliva, B, Misulovin, Z, Rothstein, D M, ...
Tetracycline analogs fell into two classes on the basis of their mode of action. Tetracycline, chlortetracycline, minocycline, doxycycline, and 6-demethyl-6-deoxytetracycline inhibited cell-free...
Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli.
Plotch, S J, Palant, O, Gluzman, Y
A cDNA clone encoding the RNA polymerase of poliovirus has been expressed in Escherichia coli under the transcriptional control of a T7 bacteriophage promoter. The poliovirus enzyme was designed to...
Jones, T R, Muzithras, V P, Gluzman, Y
The US6 gene family, located within the unique short region (US) of the human cytomegalovirus (HCMV) genome, contains six open reading frames (US6 through US11) which may encode glycoproteins, such...
Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
Mohr, I J, Fairman, M P, Stillman, B, Gluzman, Y
The biochemical activities of a series of transformation-competent, replication-defective large T-antigen point mutants were examined. The assays employed reflect partial reactions required for the...
Analysis of nonpermissivity in mouse cells overexpressing simian virus 40 T antigen.
Gerard, R D, Guggenheimer, R A, Gluzman, Y
To analyze the nature of the nonpermissivity of mouse cells for simian virus 40 (SV40) DNA replication, we isolated mouse cells producing SV40 T antigen (Tag) at levels equal to or greater than that...
To study the role of the biochemical and physiological activities of simian virus 40 (SV40) large T antigen in the lytic and transformation processes, we have analyzed DNA replication-defective,...
Van Doren, K, Hanahan, D, Gluzman, Y
Recombinant viral genomes carrying a selectable drug resistance marker have been constructed by insertion of a hybrid gene for neomycin resistance into the helper-independent adenovirus vector, delta...
Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
Strauss, M, Argani, P, Mohr, I J, Gluzman, Y
The origin-specific DNA-binding domain of simian virus 40 large T antigen was analyzed, and its C-terminal boundary was found to be at or before amino acid 259. This does not include the zinc finger...
Palindromic adenovirus type 5-simian virus 40 hybrid.
A family of novel adenovirus type 5-simian virus 40 (Ad5-SV40) recombinants (Ad5++D1) whose genomes consist of symmetrically inverted structures was isolated. Particles of Ad5++D1 could contain one...
Rasmussen, B A, Gluzman, Y, Tally, F P
One of the two major mechanisms of tetracycline resistance is ribosomal protection. Of this resistance type, tet(M) is the best characterized. Although the mechanism of tet(M) resistance has not yet...
Maulbecker, C, Mohr, I, Gluzman, Y, Bartholomew, J, Botchan, M
We describe a new complementation function within the simian virus 40 (SV40) A gene. This function is required for viral DNA replication and virus production in vivo but, surprisingly, does not...
Mohr, I J, Gluzman, Y, Fairman, M P, Strauss, M, McVey, D, Stillman, B, ...
A bacterial expression system was used to produce simian virus 40 large tumor antigen (T antigen) in the absence of the extensive posttranslational modifications that occur in mammalian cells....
Ghosh, P K, Lebowitz, P, Frisque, R J, Gluzman, Y
The 5' termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template. The 5'...
Sequence of the junction in adenovirus 2-SV40 hybrids: examples of illegitimate recombination.
Ling, L E, Manos, M M, Gluzman, Y
The nucleotide sequences of six Ad2-SV40 junctions from three Ad2-SV40 hybrid viruses (Ad2++HEY, Ad2++LEY and Ad2+D1) were determined. Comparison of parental adenovirus 2 and SV40 DNA sequences with...
Expression of early genes of origin-defective mutants of simian virus 40.
Gluzman, Y, Sambrook, J F, Frisque, R J
The nucleotide sequences of eight origin-defective mutants of simian virus 40 have been determined. All of the mutants have suffered deletions, which range in size from 4 to 241 nucleotides. Some of...
Altered restriction endonuclease cleavage pattern of simian virus 40 DNA.
Three different groups of temperature-sensitive mutants of simian virus 40, isolated and characterized by Chou and Martin (J. Virol. 13:1101--1109, 1974), have been analyzed by using restriction...
Cellular and cell-free synthesis of simian virus 40 T-antigens in permissive and transformed cells.
Prives, C, Gluzman, Y, Winocour, E
mRNA extracted from a variety of simian virus 40 (SV40)-infected monkey cell lines directs the cell-free synthesis of viral T-antigen polypeptides with molecular weights estimated as 90,000 and...
Properties of the DNA-binding domain of the simian virus 40 large T antigen.
McVey, D, Strauss, M, Gluzman, Y
T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these...
Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
Soluble extracts prepared from the nucleus and cytoplasm of human 293 cells are capable of efficient replication and supercoiling of added DNA templates that contain the origin of simian virus 40...
One boundary of the minimal origin of replication of simian virus 40 DNA lies within the A + T-rich region. Deletion of only a few bases into the adenine-thymine (AT) stretch results in a DNA...
Construction of a helper-free recombinant adenovirus that expresses polyomavirus large T antigen.
Massie, B, Gluzman, Y, Hassell, J A
Adenovirus-polyomavirus recombinant viruses were constructed in vitro by inserting a hybrid transcription unit composed of the adenovirus type 2 major late promoter and the early coding region of...
A variant of the adenovirus type 5 genome which lacks EcoRI sites has been cloned in a bacterial plasmid after the addition of EcoRI oligonucleotide linkers to its ends. Closed circular forms of the...
The large T antigen of simian virus 40 (SV40) is a multifunctional protein that is essential in both the virus lytic cycle and the oncogenic transformation of cells by SV40. To investigate the role...
Efficient transformation of human fibroblasts by adenovirus-simian virus 40 recombinants.
The origin-defective simian virus 40 (SV40) mutant 6-1 has been useful in transforming human cells (Small et al., Nature [London] 296:671-672, 1982; Nagata et al., Nature [London] 306:597-599, 1983)....
New host cell system for regulated simian virus 40 DNA replication.
Transformed monkey cell lines (CMT and BMT) that inducible express simian virus 40 (SV40) T antigen from the metallothionein promoter have been isolated and characterized. Immunoprecipitation of...
DNA-binding properties of simian virus 40 T-antigen mutants defective in viral DNA replication.
Prives, C, Covey, L, Scheller, A, Gluzman, Y
Three simian virus 40 (SV40)-transformed monkey cell lines, C2, C6, and C11, producing T-antigen variants that are unable to initiate viral DNA replication, were analyzed with respect to their...
Transfer of genes into hematopoietic cells using recombinant DNA viruses.
Karlsson, S, Humphries, R K, Gluzman, Y, Nienhuis, A W
The ability of recombinant DNA viruses to transfer genes into hematopoietic cells has been explored. A recombinant simian virus 40 (SV40) in which the early region had been replaced with the...
A herpesvirus genetic element which affects translation in the absence of the viral GADD34 function.
Novel suppressor variants of conditionally lethal HSV-1 gamma34.5 deletion mutants have been isolated which exhibit restored ability to grow on neoplastic neuronal cells. Deletion of the viral...
Properties of permissive monkey cells transformed by UV-irradiated simian virus 40.
Gluzman, Y, Davison, J, Oren, M, Winocour, E
African green monkey cells (CV1 line) were infected with UV-irradiated simian virus 40 (SV40), and permissive lines of stably transformed cells were established. These cell lines display the SV40...
Gluzman, Y., Kuff, E. L., Winocour, E.
Passage of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 at the permissive temperature in each of three permissive lines of SV40-transformed monkey CV1 cells resulted in the...
Vogel, T., Gluzman, Y., Winocour, E.
The genome of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol....
Regulation of SV40 DNA replication by phosphorylation of T antigen.
Mohr, I J, Stillman, B, Gluzman, Y
The role of phosphorylation in regulating the biochemical properties of SV40 large T antigen has been examined. Treatment of purified T antigen with calf intestinal alkaline phosphatase resulted in...
T antigen and template requirements for SV40 DNA replication in vitro.
Stillman, B, Gerard, R D, Guggenheimer, R A, Gluzman, Y
A cell-free system for replication of SV40 DNA was used to assess the effect of mutations altering either the SV40 origin of DNA replication or the virus-encoded large tumor (T) antigen. Plasmid DNAs...
Stable gene transfer and tissue-specific expression of a human globin gene using adenoviral vectors.
Karlsson, S, Van Doren, K, Schweiger, S G, Nienhuis, A W, Gluzman, Y
Helper-free double recombinant adenoviruses containing a genomic human globin gene and the neomycin resistance gene (neoR) have been constructed. The inserted globin and neoR genes are stable and...
Basolo, F., Fiore, L., Calvo, S., Falcone, V., Conaldi, P. G., Fontanini, G., ...
Mammary epithelial cells (MECs) were isolated and cultured from mammary glands of healthy women undergoing reduction mammoplasty. Normal MECs were infected with the transforming hybrid virus...