Targeting of GRASP65 to the Golgi apparatus (2001)
Yoshimura,S., Misumi,Y., Ikehara,Y., Barr,F. A., Ohno,H., Mihara,K., ...
Targeting of GRASP65 to the Golgi apparatus (2001)
Yoshimura, S., Misumi, Y., Ikehara, Y., Barr, F. A., Ohno, H., Mihara, K., ...
Molecular behavior of mutant Lewis enzymes in vivo (1999)
Nishihara, S, Hiraga, T, Ikehara, Y, Iwasaki, H, Kudo, T, Yazawa, S, ...
Ikehara, Y, Kojima, N, Kurosawa, N, Kudo, T, Kono, M, Nishihara, S, ...
Kato, Y, Nakashima, K, Iwamoto, M, Murakami, H, Hiranuma, H, Koike, T, ...
The effect of IL-1 on expression of the mineralization-related phenotype by chondrocytes was examined. In cultures of rabbit growth plate chondrocytes, IL-1 beta at 0.1 ng/ml caused 95% decreases in...
Oda, K, Fujiwara, T, Ikehara, Y
We have studied the effect of brefeldin A (BFA) on the intracellular transport of the envelope proteins of vesicular stomatitis virus (VSV) and sindbis virus in primary cultured rat hepatocytes. BFA...
Oda, K, Cheng, J, Saku, T, Takami, N, Sohda, M, Misumi, Y, ...
Placental alkaline phosphatase (PLAP) is initially synthesized as a precursor (proPLAP) with a C-terminal extension. We constructed a recombinant cDNA which encodes a chimeric protein (alpha GL-PLAP)...
Redman, C A, Thomas-Oates, J E, Ogata, S, Ikehara, Y, Ferguson, M A
The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting...
Kominami, T, Miki, A, Ikehara, Y
Alkaline phosphatase was solubilized from plasma membrane of rat liver with butanol-ol, bile acids or sodium deoxycholate, and electrophoretically compared with a soluble form in serum which was...
Miki, K, Ogata, S, Misumi, Y, Ikehara, Y
We investigated the carbohydrate structure of the third component of complement (C3) newly synthesized by cultured rat hepatocytes. When the cells were incubated with [3H]mannose, [3H]galactose or...
Oda, K, Koriyama, Y, Yamada, E, Ikehara, Y
We examined the effects of weakly basic amines on the secretion and post-translational modifications of secretory proteins in cultured rat hepatocytes. Weakly basic amines such as methylamine,...
Ikehara, Y, Hayashi, Y, Ogata, S, Miki, A, Kominami, T
A major glycoprotein of rat hepatoma plasma membranes was selectively released as a soluble form by incubating the membrane with phosphatidylinositol-specific phospholipase C. The soluble form...
Primary structure of rat liver alkaline phosphatase deduced from its cDNA.
Misumi, Y, Tashiro, K, Hattori, M, Sakaki, Y, Ikehara, Y
Rat liver alkaline phosphatase (ALP) was markedly induced by treatment of rats by bile-duct ligation and colchicine injection. Taking this advantage for enrichment of ALP mRNA, we constructed a...
Biosynthesis, assembly and secretion of fibrinogen in cultured rat hepatocytes.
The biosynthesis, assembly and secretion of fibrinogen were investigated in cultured rat hepatocytes which were incubated with [35S]methionine. When initial rates of the synthesis of three fibrinogen...
Cloning, structure and expression of cDNA for mouse contrapsin and a related protein.
Ohkubo, K, Ogata, S, Misumi, Y, Takami, N, Sinohara, H, Ikehara, Y
A cDNA clone (lambda MC-2) for contrapsin, a serine-proteinase inhibitor, was isolated from a lambda ZAP mouse liver cDNA library. The 1.6 kb cDNA insert of lambda MC-2 contained an open reading...
A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-delta 1.
Kanematsu, T, Misumi, Y, Watanabe, Y, Ozaki, S, Koga, T, Iwanaga, S, ...
We have reported that two inositol 1,4,5-trisphosphate binding proteins, with molecular masses of 85 and 130 kDa, were purified from rat brain; the former protein was found to be the delta...
Oda, K, Wada, I, Takami, N, Fujiwara, T, Misumi, Y, Ikehara, Y
When fused in-frame with a C-terminal propeptide of placental alkaline phosphatase (PLAP), rat alpha 2u-globulin (alpha GL), a nonglycosylated secretory protein, was expressed on the cell surface as...
Takeuchi, H, Kanematsu, T, Misumi, Y, Yaakob, H B, Yagisawa, H, Ikehara, Y, ...
We have previously identified a novel 130 kDa protein (p130) which binds Ins(1,4,5)P3 and shares 38% sequence identity with phospholipase C-delta 1 [Kanematsu, Misumi, Watanabe, Ozaki, Koga, Iwanaga,...
Ca2+-dependent interaction of the growth-associated protein GAP-43 with the synaptic core complex.
Haruta, T, Takami, N, Ohmura, M, Misumi, Y, Ikehara, Y
The synaptic vesicle exocytosis occurs by a highly regulated mechanism: syntaxin and 25 kDa synaptosome-associated protein (SNAP-25) are assembled with vesicle-associated membrane protein (VAMP) to...
Tsujioka, H, Takami, N, Misumi, Y, Ikehara, Y
Many proteins are anchored to the cell membrane by glycosylphosphatidylinositol (GPI). One of the functions proposed for the GPI anchor is as a possible mediator in signal transduction through its...
Fukushi-Irié, M, Ito, M, Amaya, Y, Amizuka, N, Ozawa, H, Omura, S, ...
Tissue-non-specific alkaline phosphatase (TNSALP) with an Arg(54)-->Cys (R54C) or an Asp(277)-->Ala (D277A)substitution was found in a patient with hypophosphatasia [Henthorn,Raducha, Fedde, Lafferty...
Cell proliferation and renewal of mouse adrenal cortex.
Kataoka, Y, Ikehara, Y, Hattori, T
Although many hypotheses concerning cell proliferation and renewal in the adrenal cortex of mammals have been proposed, this topic has so far not been elucidated. Adrenocortical cells of adult...
Kishi, K, Yasuda, T, Ikehara, Y, Sawazaki, K, Sato, W, Iida, R
We have devised a zymogram method with high sensitivity and resolution for investigating molecular heterogeneity and genetic polymorphism of deoxyribonuclease I. A combination technique of...
Kato, Y, Nakashima, K, Iwamoto, M, Murakami, H, Hiranuma, H, Koike, T, ...
The effect of IL-1 on expression of the mineralization-related phenotype by chondrocytes was examined. In cultures of rabbit growth plate chondrocytes, IL-1 beta at 0.1 ng/ml caused 95% decreases in...
Oda, K, Fujiwara, T, Ikehara, Y
We have studied the effect of brefeldin A (BFA) on the intracellular transport of the envelope proteins of vesicular stomatitis virus (VSV) and sindbis virus in primary cultured rat hepatocytes. BFA...
Oda, K, Cheng, J, Saku, T, Takami, N, Sohda, M, Misumi, Y, ...
Placental alkaline phosphatase (PLAP) is initially synthesized as a precursor (proPLAP) with a C-terminal extension. We constructed a recombinant cDNA which encodes a chimeric protein (alpha GL-PLAP)...
Redman, C A, Thomas-Oates, J E, Ogata, S, Ikehara, Y, Ferguson, M A
The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting...
Kominami, T, Miki, A, Ikehara, Y
Alkaline phosphatase was solubilized from plasma membrane of rat liver with butanol-ol, bile acids or sodium deoxycholate, and electrophoretically compared with a soluble form in serum which was...
Miki, K, Ogata, S, Misumi, Y, Ikehara, Y
We investigated the carbohydrate structure of the third component of complement (C3) newly synthesized by cultured rat hepatocytes. When the cells were incubated with [3H]mannose, [3H]galactose or...
Oda, K, Koriyama, Y, Yamada, E, Ikehara, Y
We examined the effects of weakly basic amines on the secretion and post-translational modifications of secretory proteins in cultured rat hepatocytes. Weakly basic amines such as methylamine,...
Ikehara, Y, Hayashi, Y, Ogata, S, Miki, A, Kominami, T
A major glycoprotein of rat hepatoma plasma membranes was selectively released as a soluble form by incubating the membrane with phosphatidylinositol-specific phospholipase C. The soluble form...
Primary structure of rat liver alkaline phosphatase deduced from its cDNA.
Misumi, Y, Tashiro, K, Hattori, M, Sakaki, Y, Ikehara, Y
Rat liver alkaline phosphatase (ALP) was markedly induced by treatment of rats by bile-duct ligation and colchicine injection. Taking this advantage for enrichment of ALP mRNA, we constructed a...
Biosynthesis, assembly and secretion of fibrinogen in cultured rat hepatocytes.
The biosynthesis, assembly and secretion of fibrinogen were investigated in cultured rat hepatocytes which were incubated with [35S]methionine. When initial rates of the synthesis of three fibrinogen...
Cloning, structure and expression of cDNA for mouse contrapsin and a related protein.
Ohkubo, K, Ogata, S, Misumi, Y, Takami, N, Sinohara, H, Ikehara, Y
A cDNA clone (lambda MC-2) for contrapsin, a serine-proteinase inhibitor, was isolated from a lambda ZAP mouse liver cDNA library. The 1.6 kb cDNA insert of lambda MC-2 contained an open reading...
Cell proliferation and renewal of mouse adrenal cortex.
Kataoka, Y, Ikehara, Y, Hattori, T
Although many hypotheses concerning cell proliferation and renewal in the adrenal cortex of mammals have been proposed, this topic has so far not been elucidated. Adrenocortical cells of adult...
A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-delta 1.
Kanematsu, T, Misumi, Y, Watanabe, Y, Ozaki, S, Koga, T, Iwanaga, S, ...
We have reported that two inositol 1,4,5-trisphosphate binding proteins, with molecular masses of 85 and 130 kDa, were purified from rat brain; the former protein was found to be the delta...
Oda, K, Wada, I, Takami, N, Fujiwara, T, Misumi, Y, Ikehara, Y
When fused in-frame with a C-terminal propeptide of placental alkaline phosphatase (PLAP), rat alpha 2u-globulin (alpha GL), a nonglycosylated secretory protein, was expressed on the cell surface as...
Takeuchi, H, Kanematsu, T, Misumi, Y, Yaakob, H B, Yagisawa, H, Ikehara, Y, ...
We have previously identified a novel 130 kDa protein (p130) which binds Ins(1,4,5)P3 and shares 38% sequence identity with phospholipase C-delta 1 [Kanematsu, Misumi, Watanabe, Ozaki, Koga, Iwanaga,...
Ca2+-dependent interaction of the growth-associated protein GAP-43 with the synaptic core complex.
Haruta, T, Takami, N, Ohmura, M, Misumi, Y, Ikehara, Y
The synaptic vesicle exocytosis occurs by a highly regulated mechanism: syntaxin and 25 kDa synaptosome-associated protein (SNAP-25) are assembled with vesicle-associated membrane protein (VAMP) to...
Tsujioka, H, Takami, N, Misumi, Y, Ikehara, Y
Many proteins are anchored to the cell membrane by glycosylphosphatidylinositol (GPI). One of the functions proposed for the GPI anchor is as a possible mediator in signal transduction through its...
Fukushi-Irié, M, Ito, M, Amaya, Y, Amizuka, N, Ozawa, H, Omura, S, ...
Tissue-non-specific alkaline phosphatase (TNSALP) with an Arg(54)-->Cys (R54C) or an Asp(277)-->Ala (D277A)substitution was found in a patient with hypophosphatasia [Henthorn,Raducha, Fedde, Lafferty...
Kishi, K, Yasuda, T, Ikehara, Y, Sawazaki, K, Sato, W, Iida, R
We have devised a zymogram method with high sensitivity and resolution for investigating molecular heterogeneity and genetic polymorphism of deoxyribonuclease I. A combination technique of...
Yokoyama, H, Ikehara, Y, Kodera, Y, Ikehara, S, Yatabe, Y, Mochizuki, Y, ...
Gastric cancer metastasised to the liver was found to overexpress HER2 at a significantly higher incidence than primary gastric cancers. The purpose of the present study was to investigate the...