Y. Ishizaki

Programmed cell death by default in embryonic cells, fibroblasts, and cancer cells.

Ishizaki, Y, Cheng, L, Mudge, A W, Raff, M C

We recently proposed that most mammalian cells constitutively express all of the proteins required to undergo programmed cell death (PCD) and undergo PCD unless continuously signaled by other cells...

Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol.

Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A

Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and...

Stimulation of intermolecular ligation with E. coli DNA ligase by high concentrations of monovalent cations in polyethylene glycol solutions.

Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A

In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt...

Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase.

Kotani, H, Ishizaki, Y, Hiraoka, N, Obayashi, A

The coding region of the gene for bacteriophage SP6 RNA polymerase was cloned into pBR322, and its entire nucleotide sequence was deduced. The predicted amino acid sequence for the polymerase...

Influence of monovalent cations on the activity of T4 DNA ligase in the presence of polyethylene glycol.

Hayashi, K, Nakazawa, M, Ishizaki, Y, Obayashi, A

Monovalent cations such as Na+ and K+ inhibit the activity of T4 DNA ligase. However, the extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate; in many cases,...

Immunohistochemical detection of imidazolone, a novel advanced glycation end product, in kidneys and aortas of diabetic patients.

Niwa, T, Katsuzaki, T, Miyazaki, S, Miyazaki, T, Ishizaki, Y, Hayase, F, ...

To investigate the role of the Maillard reaction in the pathogenesis of diabetic complications, we produced several clones of monoclonal antibodies against advanced glycation end products (AGEs) by...

Programmed cell death by default in embryonic cells, fibroblasts, and cancer cells.

Ishizaki, Y, Cheng, L, Mudge, A W, Raff, M C

We recently proposed that most mammalian cells constitutively express all of the proteins required to undergo programmed cell death (PCD) and undergo PCD unless continuously signaled by other cells...

Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol.

Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A

Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and...

Stimulation of intermolecular ligation with E. coli DNA ligase by high concentrations of monovalent cations in polyethylene glycol solutions.

Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A

In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt...

Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase.

Kotani, H, Ishizaki, Y, Hiraoka, N, Obayashi, A

The coding region of the gene for bacteriophage SP6 RNA polymerase was cloned into pBR322, and its entire nucleotide sequence was deduced. The predicted amino acid sequence for the polymerase...

Influence of monovalent cations on the activity of T4 DNA ligase in the presence of polyethylene glycol.

Hayashi, K, Nakazawa, M, Ishizaki, Y, Obayashi, A

Monovalent cations such as Na+ and K+ inhibit the activity of T4 DNA ligase. However, the extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate; in many cases,...

Immunohistochemical detection of imidazolone, a novel advanced glycation end product, in kidneys and aortas of diabetic patients.

Niwa, T, Katsuzaki, T, Miyazaki, S, Miyazaki, T, Ishizaki, Y, Hayase, F, ...

To investigate the role of the Maillard reaction in the pathogenesis of diabetic complications, we produced several clones of monoclonal antibodies against advanced glycation end products (AGEs) by...