Characterization of a second lysine decarboxylase isolated from Escherichia coli.
Kikuchi, Y, Kojima, H, Tanaka, T, Takatsuka, Y, Kamio, Y
We report here on the existence of a new gene for lysine decarboxylase in Escherichia coli K-12. The hybridization experiments with a cadA probe at low stringency showed that the homologous region of...
Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.
Sugahara, T, Konno, Y, Ohta, H, Ito, K, Kaneko, J, Kamio, Y, ...
Two alkaline phosphatases were extracted from the membranes of Bacillus subtilis 168 stationary-phase cells and purified as homogeneous proteins by hydroxyapatite column chromatography. Alkaline...
Terawaki, Y, Nozue, H, Zeng, H, Hayashi, T, Kamio, Y, Itoh, Y
We constructed a system in which wild-type RepA or RepAcop1 protein was supplied in trans in various amounts to coexisting mini-Rts1 plasmids by clones of the repA or repAcop1 gene under the control...
Terawaki, Y, Hong, Z, Itoh, Y, Kamio, Y
RepA protein, essential for replication of plasmid Rts1, was found to bind in vivo immediately upstream of the repA promoter in studies with mini-Rts1 derivatives with deletions in the upstream...
Purification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA.
Kamio, Y, Itoh, Y, Terawaki, Y
RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII...
Essential DNA sequence for the replication of Rts1.
Itoh, Y, Kamio, Y, Terawaki, Y
The promoter sequence of the mini-Rts1 repA gene encoding the 33,000-dalton RepA protein that is essential for replication was defined by RNA polymerase protection experiments and by analyzing RepA...
Veillonella alcalescens ATCC 17745, a strictly anaerobic, gram-negative small coccus, requires putrescine or cadaverine for growth (M. B. Ritchey, and E. A. Delwiche, J. Bacteriol. 124:1213-1219,...
Putrescine and cadaverine are essential constituents of the peptidoglycan of Veillonella alcalescens, Veillonella parvula, and Selenomonas ruminantium and are necessary for the growth of these...
Complete nucleotide sequence of mini-Rts1 and its copy mutant.
Kamio, Y, Tabuchi, A, Itoh, Y, Katagiri, H, Terawaki, Y
The nucleotide sequence of the whole mini-Rts1 genome consisting of 1,855 base pairs was determined. In addition to the cluster of five 24-base-pair direct repeats previously detected (Y. Kamio and...
Kamio, Y, Itoh, Y, Terawaki, Y
The peptidoglycan of Selenomonas ruminantium, a strictly anaerobic bacterium, contains cadaverine (Y. Kamio, Y. Itoh, Y. Terawaki, and T. Kusano, J. Bacteriol. 145:122-128, 1981). This report...
Cadaverine is covalently linked to peptidoglycan in Selenomonas ruminantium.
Kamio, Y, Itoh, Y, Terawaki, Y, Kusano, T
Cadaverine was found to exist as a component of cell wall peptidoglycan of Selenomonas ruminantium, a strictly anaerobic bacterium. [14C]cadaverine added to the growth medium was incorporated into...
Purification and properties of Selenomonas ruminantium lysine decarboxylase.
Selenomonas ruminantium, a strictly anaerobic, gram-negative bacterium isolated from sheep rumen, contains lysine decarboxylase (Y. Kamio et al., J. Bacteriol. 145:122-128, 1981). This report...
R plasmic Rtsl-mediated production of extracellular deoxyribonuclease in Escherichia coli.
Matsumoto, H, Kamio, Y, Kobayashi, R, Terawaki, Y
Escherichia coli strains harboring Rtsl were found to excrete extracellular deoxyribonuclease. The DNase activity was greater in cells with pTW2, a mutant from Rtsl.
The outer membrane layer of the cell wall was isolated from wild-type Salmonella typhimurium LT2 as well as from its mutants producing lipopolysaccharides with shorter saccharide chains. Chemical...
The isolation procedure and characterization of the outer and inner membranes from Selenomonas ruminatium cells, a strictly anaerobic bacterium, are described. The metabolic fate of [14C]decanoate...
Outer membrane proteins and cell surface structure of Selenomonas ruminantium.
The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first,...
Nucleotide sequence of an incompatibility region of mini-Rts1 that contains five direct repeats.
The plasmid mini-Rts1, consisting of an EcoRI/HindIII fragment of about 1.8 kilobases (kb), contains an incompatibility determinant in its EcoRI/AccI region (0.5 kb). The nucleotide sequence of this...
Characterization of a second lysine decarboxylase isolated from Escherichia coli.
Kikuchi, Y, Kojima, H, Tanaka, T, Takatsuka, Y, Kamio, Y
We report here on the existence of a new gene for lysine decarboxylase in Escherichia coli K-12. The hybridization experiments with a cadA probe at low stringency showed that the homologous region of...
Purification and properties of two membrane alkaline phosphatases from Bacillus subtilis 168.
Sugahara, T, Konno, Y, Ohta, H, Ito, K, Kaneko, J, Kamio, Y, ...
Two alkaline phosphatases were extracted from the membranes of Bacillus subtilis 168 stationary-phase cells and purified as homogeneous proteins by hydroxyapatite column chromatography. Alkaline...
Terawaki, Y, Nozue, H, Zeng, H, Hayashi, T, Kamio, Y, Itoh, Y
We constructed a system in which wild-type RepA or RepAcop1 protein was supplied in trans in various amounts to coexisting mini-Rts1 plasmids by clones of the repA or repAcop1 gene under the control...
Terawaki, Y, Hong, Z, Itoh, Y, Kamio, Y
RepA protein, essential for replication of plasmid Rts1, was found to bind in vivo immediately upstream of the repA promoter in studies with mini-Rts1 derivatives with deletions in the upstream...
Purification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA.
Kamio, Y, Itoh, Y, Terawaki, Y
RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII...
Essential DNA sequence for the replication of Rts1.
Itoh, Y, Kamio, Y, Terawaki, Y
The promoter sequence of the mini-Rts1 repA gene encoding the 33,000-dalton RepA protein that is essential for replication was defined by RNA polymerase protection experiments and by analyzing RepA...
Veillonella alcalescens ATCC 17745, a strictly anaerobic, gram-negative small coccus, requires putrescine or cadaverine for growth (M. B. Ritchey, and E. A. Delwiche, J. Bacteriol. 124:1213-1219,...
Putrescine and cadaverine are essential constituents of the peptidoglycan of Veillonella alcalescens, Veillonella parvula, and Selenomonas ruminantium and are necessary for the growth of these...
Complete nucleotide sequence of mini-Rts1 and its copy mutant.
Kamio, Y, Tabuchi, A, Itoh, Y, Katagiri, H, Terawaki, Y
The nucleotide sequence of the whole mini-Rts1 genome consisting of 1,855 base pairs was determined. In addition to the cluster of five 24-base-pair direct repeats previously detected (Y. Kamio and...
Kamio, Y, Itoh, Y, Terawaki, Y
The peptidoglycan of Selenomonas ruminantium, a strictly anaerobic bacterium, contains cadaverine (Y. Kamio, Y. Itoh, Y. Terawaki, and T. Kusano, J. Bacteriol. 145:122-128, 1981). This report...
Cadaverine is covalently linked to peptidoglycan in Selenomonas ruminantium.
Kamio, Y, Itoh, Y, Terawaki, Y, Kusano, T
Cadaverine was found to exist as a component of cell wall peptidoglycan of Selenomonas ruminantium, a strictly anaerobic bacterium. [14C]cadaverine added to the growth medium was incorporated into...
Nucleotide sequence of an incompatibility region of mini-Rts1 that contains five direct repeats.
The plasmid mini-Rts1, consisting of an EcoRI/HindIII fragment of about 1.8 kilobases (kb), contains an incompatibility determinant in its EcoRI/AccI region (0.5 kb). The nucleotide sequence of this...
Purification and properties of Selenomonas ruminantium lysine decarboxylase.
Selenomonas ruminantium, a strictly anaerobic, gram-negative bacterium isolated from sheep rumen, contains lysine decarboxylase (Y. Kamio et al., J. Bacteriol. 145:122-128, 1981). This report...
R plasmic Rtsl-mediated production of extracellular deoxyribonuclease in Escherichia coli.
Matsumoto, H, Kamio, Y, Kobayashi, R, Terawaki, Y
Escherichia coli strains harboring Rtsl were found to excrete extracellular deoxyribonuclease. The DNase activity was greater in cells with pTW2, a mutant from Rtsl.
The outer membrane layer of the cell wall was isolated from wild-type Salmonella typhimurium LT2 as well as from its mutants producing lipopolysaccharides with shorter saccharide chains. Chemical...
The isolation procedure and characterization of the outer and inner membranes from Selenomonas ruminatium cells, a strictly anaerobic bacterium, are described. The metabolic fate of [14C]decanoate...
Outer membrane proteins and cell surface structure of Selenomonas ruminantium.
The protein compositions of the membrane preparations from Selenomonas ruminantium grown in glucose or lactate medium were determined by sodium dodecyl sulfate- and two-dimensional (first,...
Sex difference in the inhaled tartaric acid cough threshold in non-atopic healthy subjects.
Fujimura, M, Sakamoto, S, Kamio, Y, Matsuda, T
The threshold for cough induced by inhaled tartaric acid was measured in 71 non-atopic healthy volunteers. The cough threshold was lower in women than in men, which may be relevant to previous...
Fujimura, M, Sakamoto, S, Kamio, Y, Matsuda, T
BACKGROUND: The direct effect of bronchoconstriction on cough receptor sensitivity is unknown, and the antitussive effect of beta 2 adrenergic agonists in man has been controversial. This study was...
Fujimura, M, Sakamoto, S, Kamio, Y, Bando, T, Kurashima, K, Matsuda, T
BACKGROUND--To evaluate the effect of inhaled beta 2 adrenergic agonists on the sensitivity of airway cough receptors, the effect of inhaled procaterol on cough induced by aerosolised capsaicin, a...
Effect of oral mexiletine on the cough response to capsaicin and tartaric acid
Fujimura, M., Kamio, Y., Myou, S., Hashimoto, T.
BACKGROUND—The effect of the orally active local anaesthetic mexiletine on the cough response to two different tussive agents, a C-fibre ending stimulator capsaicin and a chemostimulant tartaric...