Nakafuku, M, Nagamine, M, Ohtoshi, A, Tanaka, K, Toh-e, A, Kaziro, Y
NF1 was first identified as the gene responsible for the pathogenesis of the human genetic disorder neurofibromatosis type 1. cDNA cloning revealed that its putative protein product has a domain...
Koide, H, Satoh, T, Nakafuku, M, Kaziro, Y
Ras is involved in signal transduction of various factors for growth, differentiation, and oncogenesis. Recent studies have revealed several proteins that function upstream and downstream of the Ras...
Tsukamoto, T, Toyama, R, Itoh, H, Kozasa, T, Matsuoka, M, Kaziro, Y
Go is a specific class ("other") of signal-transducing heterotrimeric GTP-binding proteins (G proteins) that is expressed in high levels in mammalian brain. We have cloned two different rat cDNAs...
Satoh, T, Nakafuku, M, Miyajima, A, Kaziro, Y
The protooncogene ras acts as a component of signal-transduction networks in many kinds of cells. The ras gene product (p21) is a GTP-binding protein, and the activity of the protein is regulated by...
Obara, T, Nakafuku, M, Yamamoto, M, Kaziro, Y
The gpal gene of Schizosaccharomyces pombe, which encodes a protein homologous with the alpha subunits of mammalian guanine nucleotide-binding proteins (G proteins), was isolated by...
Satoh, T, Endo, M, Nakafuku, M, Nakamura, S, Kaziro, Y
The ras gene product (p21) is a GTP-binding protein and is thought to play an important role in signal transduction of growth and differentiation in many types of mammalian cells. The p21.GTP complex...
Satoh, T, Endo, M, Nakafuku, M, Akiyama, T, Yamamoto, T, Kaziro, Y
The ras gene product (p21) is a GTP-binding protein and has been thought to transduce signals regulating proliferation or differentiation of cells. Like other GTP-binding proteins, p21.GTP is an...
Nakafuku, M, Obara, T, Kaibuchi, K, Miyajima, I, Miyajima, A, Itoh, H, ...
In a previous paper, we demonstrated that a gene coding for a protein homologous to the alpha subunit of mammalian guanine nucleotide-binding regulatory (G) proteins occurs in Saccharomyces...
Isolation and characterization of the human Gs alpha gene.
Kozasa, T, Itoh, H, Tsukamoto, T, Kaziro, Y
The gene for Gs alpha (the alpha subunit of the guanine nucleotide-binding protein Gs) was isolated from human genomic libraries using rat Gs alpha cDNA as a probe. Comparison of the nucleotide...
Matsuoka, M, Itoh, H, Kozasa, T, Kaziro, Y
We have isolated cDNA clones from rat C6 glioma cells coding for several guanine nucleotide-binding regulatory protein (G protein) alpha subunits (G alpha). The cDNA clones were then used to isolate...
MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae.
Ruggieri, R, Tanaka, K, Nakafuku, M, Kaziro, Y, Toh-e, A, Matsumoto, K
We have previously demonstrated that the IRA1-encoded protein inhibits the function of the RAS protein in a fashion antagonistic to the function of the CDC25 protein in the RAS-cAMP pathway in...
Nakafuku, M, Itoh, H, Nakamura, S, Kaziro, Y
From cross-hybridization studies with cDNAs that code for the alpha subunits of rat brain guanine nucleotide-binding regulatory (G) proteins, we have isolated a gene from yeast Saccharomyces...
Mizumoto, K, Kaziro, Y, Lipmann, F
Rat liver RNA guanylyltransferase catalyzes a GTP-PPi exchange reaction in the absence of acceptor RNA [Mizumoto, K. & Lipmann, F. (1979) Proc. Natl. Acad. Sci. USA 76, 4961-4965] suggesting that the...
Primary structure of elongation factor Tu from Escherichia coli.
Arai, K, Clark, B F, Duffy, L, Jones, M D, Kaziro, Y, Laursen, R A, ...
The amino acid sequence of elongation factor Tu (EF-Tu) from Escherichia coli has been determined. EF-Tu is a single-chain polypeptide composed of 393 amino acids (Mr 43,225 for the species bearing...
Satoh, T, Fantl, W J, Escobedo, J A, Williams, L T, Kaziro, Y
A series of pieces of evidence have shown that Ras protein acts as a transducer of the platelet-derived growth factor (PDGF) receptor-mediated signaling pathway: (i) formation of Ras.GTP is detected...
Tanaka, K, Nakafuku, M, Tamanoi, F, Kaziro, Y, Matsumoto, K, Toh-e, A
The IRA1 gene is a negative regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. To identify other genes involved in this pathway, we screened yeast genomic DNA libraries for genes...
Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.
Satoh, T, Nakamura, S, Taga, T, Matsuda, T, Hirano, T, Kishimoto, T, ...
B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is...
Hattori, S, Clanton, D J, Satoh, T, Nakamura, S, Kaziro, Y, Kawakita, M, ...
The neutralizing monoclonal antibody Y13-259 severely hampers the nucleotide exchange reaction between p21-bound and exogenous guanine nucleotides but does not interfere with the association of GDP...
Nakayama, N, Kaziro, Y, Arai, K, Matsumoto, K
The ste mutants (ste2, ste4, ste5, ste7, ste11, and ste12) are insensitive to mating factors and are, therefore, sterile. Roles of the STE gene products in the GPA1-mediated mating factor signaling...
Satoh, T, Nakamura, S, Kaziro, Y
Rat pheochromocytoma (PC12) cells differentiate to neuronal cells in response to nerve growth factor. It has been shown that microinjection of oncogenic but not proto-oncogenic p21 protein induces...
Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor.
Tsuchiya, M, Asano, S, Kaziro, Y, Nagata, S
A cDNA sequence coding for murine granulocyte colony-stimulating factor (G-CSF) has been isolated from a cDNA library prepared with mRNA derived from murine fibrosarcoma NFSA cells, which produce...
The 3.6-kilobase Bgl II-EcoRI fragment from R1 plasmid containing copA, repA, and the replication origin (ori) was inserted into the ColE1-type plasmid pUC8. The resulting hybrid plasmid replicates...
Molecular cloning and nucleotide sequence of cDNA for rat ornithine carbamoyltransferase precursor.
Takiguchi, M, Miura, S, Mori, M, Tatibana, M, Nagata, S, Kaziro, Y
Messenger RNA of rat ornithine carbamoyltransferase (EC 2.1.3.3), a mitochondrial matrix enzyme, was enriched by immunoprecipitation of rat liver free polysomes, and recombinant plasmids were...
Nagata, S, Tsunetsugu-Yokota, Y, Naito, A, Kaziro, Y
A 3.1-kilobase Bgl II fragment of Saccharomyces cerevisiae carrying the nuclear gene encoding the mitochondrial polypeptide chain elongation factor (EF) Tu has been cloned on pBR327 to yield a...
Molecular cloning and expression of a cDNA encoding the secretin receptor.
Ishihara, T, Nakamura, S, Kaziro, Y, Takahashi, T, Takahashi, K, Nagata, S
Secretin is a 27 amino acid peptide which stimulates the secretion of bicarbonate, enzymes and potassium ion from the pancreas. A complementary DNA encoding the rat secretin receptor was isolated...
Characterization of recombinant human granulocyte-colony-stimulating factor produced in mouse cells.
Tsuchiya, M, Nomura, H, Asano, S, Kaziro, Y, Nagata, S
Mouse C127I cells were transformed with a chimeric plasmid consisting of bovine papillomavirus DNA and human granulocyte-colony-stimulating factor (G-CSF) cDNA placed under the control of the SV40...
Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe.
We have cloned a ras gene homologue from fission yeast Schizosaccharomyces pombe and determined its nucleotide sequence. A putative coding sequence for 219 amino acids was found. The sequence...
Mizushima-Sugano, J, Kaziro, Y
We have located the DNA sequence involved in the stringent control of the Escherichia coli tufB operon. Various deletion and insertion mutants of the promoter locus were constructed by in vitro...
Yagi, Y, Mizumoto, K, Kaziro, Y
An RNA 5'-triphosphatase activity hydrolyzing gamma-phosphate from pppN-RNA was found to be associated with mRNA guanylyltransferase partially purified from rat liver nuclei. The activity...
Toyama, R, Mizumoto, K, Nakahara, Y, Tatsuno, T, Kaziro, Y
The mRNA capping reaction catalyzed by rat liver mRNA guanylyltransferase proceeds through an enzyme-GMP intermediate in which GMP is linked to the enzyme by a phosphoamide linkage. The studies...
Nagata, S, Nagashima, K, Tsunetsugu-Yokota, Y, Fujimura, K, Miyazaki, M, Kaziro, Y
Messenger RNA for yeast cytosolic polypeptide chain elongation factor 1 alpha (EF-1 alpha) was partially purified from Saccharomyces cerevisiae. Double-stranded complementary DNA (cDNA) was...
Suppressors of a Gpa1 Mutation Cause Sterility in Saccharomyces Cerevisiae
Miyajima, I., Nakayama, N., Nakafuku, M., Kaziro, Y., Arai, K. I., Matsumoto, K.
The Saccharomyces cerevisiae GPA1 gene encodes a protein highly homologous to the α subunit of mammalian G proteins and is essential for haploid cell growth. We have selected 77 mutants able to...
Nakafuku, M, Nagamine, M, Ohtoshi, A, Tanaka, K, Toh-e, A, Kaziro, Y
NF1 was first identified as the gene responsible for the pathogenesis of the human genetic disorder neurofibromatosis type 1. cDNA cloning revealed that its putative protein product has a domain...
Koide, H, Satoh, T, Nakafuku, M, Kaziro, Y
Ras is involved in signal transduction of various factors for growth, differentiation, and oncogenesis. Recent studies have revealed several proteins that function upstream and downstream of the Ras...
Tsukamoto, T, Toyama, R, Itoh, H, Kozasa, T, Matsuoka, M, Kaziro, Y
Go is a specific class ("other") of signal-transducing heterotrimeric GTP-binding proteins (G proteins) that is expressed in high levels in mammalian brain. We have cloned two different rat cDNAs...
Satoh, T, Nakafuku, M, Miyajima, A, Kaziro, Y
The protooncogene ras acts as a component of signal-transduction networks in many kinds of cells. The ras gene product (p21) is a GTP-binding protein, and the activity of the protein is regulated by...
Obara, T, Nakafuku, M, Yamamoto, M, Kaziro, Y
The gpal gene of Schizosaccharomyces pombe, which encodes a protein homologous with the alpha subunits of mammalian guanine nucleotide-binding proteins (G proteins), was isolated by...
Satoh, T, Endo, M, Nakafuku, M, Nakamura, S, Kaziro, Y
The ras gene product (p21) is a GTP-binding protein and is thought to play an important role in signal transduction of growth and differentiation in many types of mammalian cells. The p21.GTP complex...
Satoh, T, Endo, M, Nakafuku, M, Akiyama, T, Yamamoto, T, Kaziro, Y
The ras gene product (p21) is a GTP-binding protein and has been thought to transduce signals regulating proliferation or differentiation of cells. Like other GTP-binding proteins, p21.GTP is an...
Nakafuku, M, Obara, T, Kaibuchi, K, Miyajima, I, Miyajima, A, Itoh, H, ...
In a previous paper, we demonstrated that a gene coding for a protein homologous to the alpha subunit of mammalian guanine nucleotide-binding regulatory (G) proteins occurs in Saccharomyces...
Isolation and characterization of the human Gs alpha gene.
Kozasa, T, Itoh, H, Tsukamoto, T, Kaziro, Y
The gene for Gs alpha (the alpha subunit of the guanine nucleotide-binding protein Gs) was isolated from human genomic libraries using rat Gs alpha cDNA as a probe. Comparison of the nucleotide...
Matsuoka, M, Itoh, H, Kozasa, T, Kaziro, Y
We have isolated cDNA clones from rat C6 glioma cells coding for several guanine nucleotide-binding regulatory protein (G protein) alpha subunits (G alpha). The cDNA clones were then used to isolate...
MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae.
Ruggieri, R, Tanaka, K, Nakafuku, M, Kaziro, Y, Toh-e, A, Matsumoto, K
We have previously demonstrated that the IRA1-encoded protein inhibits the function of the RAS protein in a fashion antagonistic to the function of the CDC25 protein in the RAS-cAMP pathway in...
Nakafuku, M, Itoh, H, Nakamura, S, Kaziro, Y
From cross-hybridization studies with cDNAs that code for the alpha subunits of rat brain guanine nucleotide-binding regulatory (G) proteins, we have isolated a gene from yeast Saccharomyces...
Mizumoto, K, Kaziro, Y, Lipmann, F
Rat liver RNA guanylyltransferase catalyzes a GTP-PPi exchange reaction in the absence of acceptor RNA [Mizumoto, K. & Lipmann, F. (1979) Proc. Natl. Acad. Sci. USA 76, 4961-4965] suggesting that the...
Primary structure of elongation factor Tu from Escherichia coli.
Arai, K, Clark, B F, Duffy, L, Jones, M D, Kaziro, Y, Laursen, R A, ...
The amino acid sequence of elongation factor Tu (EF-Tu) from Escherichia coli has been determined. EF-Tu is a single-chain polypeptide composed of 393 amino acids (Mr 43,225 for the species bearing...
Satoh, T, Fantl, W J, Escobedo, J A, Williams, L T, Kaziro, Y
A series of pieces of evidence have shown that Ras protein acts as a transducer of the platelet-derived growth factor (PDGF) receptor-mediated signaling pathway: (i) formation of Ras.GTP is detected...
Tanaka, K, Nakafuku, M, Tamanoi, F, Kaziro, Y, Matsumoto, K, Toh-e, A
The IRA1 gene is a negative regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae. To identify other genes involved in this pathway, we screened yeast genomic DNA libraries for genes...
Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.
Satoh, T, Nakamura, S, Taga, T, Matsuda, T, Hirano, T, Kishimoto, T, ...
B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is...
Hattori, S, Clanton, D J, Satoh, T, Nakamura, S, Kaziro, Y, Kawakita, M, ...
The neutralizing monoclonal antibody Y13-259 severely hampers the nucleotide exchange reaction between p21-bound and exogenous guanine nucleotides but does not interfere with the association of GDP...
Nakayama, N, Kaziro, Y, Arai, K, Matsumoto, K
The ste mutants (ste2, ste4, ste5, ste7, ste11, and ste12) are insensitive to mating factors and are, therefore, sterile. Roles of the STE gene products in the GPA1-mediated mating factor signaling...
Satoh, T, Nakamura, S, Kaziro, Y
Rat pheochromocytoma (PC12) cells differentiate to neuronal cells in response to nerve growth factor. It has been shown that microinjection of oncogenic but not proto-oncogenic p21 protein induces...
Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor.
Tsuchiya, M, Asano, S, Kaziro, Y, Nagata, S
A cDNA sequence coding for murine granulocyte colony-stimulating factor (G-CSF) has been isolated from a cDNA library prepared with mRNA derived from murine fibrosarcoma NFSA cells, which produce...
The 3.6-kilobase Bgl II-EcoRI fragment from R1 plasmid containing copA, repA, and the replication origin (ori) was inserted into the ColE1-type plasmid pUC8. The resulting hybrid plasmid replicates...
Molecular cloning and nucleotide sequence of cDNA for rat ornithine carbamoyltransferase precursor.
Takiguchi, M, Miura, S, Mori, M, Tatibana, M, Nagata, S, Kaziro, Y
Messenger RNA of rat ornithine carbamoyltransferase (EC 2.1.3.3), a mitochondrial matrix enzyme, was enriched by immunoprecipitation of rat liver free polysomes, and recombinant plasmids were...
Nagata, S, Tsunetsugu-Yokota, Y, Naito, A, Kaziro, Y
A 3.1-kilobase Bgl II fragment of Saccharomyces cerevisiae carrying the nuclear gene encoding the mitochondrial polypeptide chain elongation factor (EF) Tu has been cloned on pBR327 to yield a...
Molecular cloning and expression of a cDNA encoding the secretin receptor.
Ishihara, T, Nakamura, S, Kaziro, Y, Takahashi, T, Takahashi, K, Nagata, S
Secretin is a 27 amino acid peptide which stimulates the secretion of bicarbonate, enzymes and potassium ion from the pancreas. A complementary DNA encoding the rat secretin receptor was isolated...
Characterization of recombinant human granulocyte-colony-stimulating factor produced in mouse cells.
Tsuchiya, M, Nomura, H, Asano, S, Kaziro, Y, Nagata, S
Mouse C127I cells were transformed with a chimeric plasmid consisting of bovine papillomavirus DNA and human granulocyte-colony-stimulating factor (G-CSF) cDNA placed under the control of the SV40...
Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe.
We have cloned a ras gene homologue from fission yeast Schizosaccharomyces pombe and determined its nucleotide sequence. A putative coding sequence for 219 amino acids was found. The sequence...
Mizushima-Sugano, J, Kaziro, Y
We have located the DNA sequence involved in the stringent control of the Escherichia coli tufB operon. Various deletion and insertion mutants of the promoter locus were constructed by in vitro...
Yagi, Y, Mizumoto, K, Kaziro, Y
An RNA 5'-triphosphatase activity hydrolyzing gamma-phosphate from pppN-RNA was found to be associated with mRNA guanylyltransferase partially purified from rat liver nuclei. The activity...
Toyama, R, Mizumoto, K, Nakahara, Y, Tatsuno, T, Kaziro, Y
The mRNA capping reaction catalyzed by rat liver mRNA guanylyltransferase proceeds through an enzyme-GMP intermediate in which GMP is linked to the enzyme by a phosphoamide linkage. The studies...
Nagata, S, Nagashima, K, Tsunetsugu-Yokota, Y, Fujimura, K, Miyazaki, M, Kaziro, Y
Messenger RNA for yeast cytosolic polypeptide chain elongation factor 1 alpha (EF-1 alpha) was partially purified from Saccharomyces cerevisiae. Double-stranded complementary DNA (cDNA) was...
Suppressors of a Gpa1 Mutation Cause Sterility in Saccharomyces Cerevisiae
Miyajima, I., Nakayama, N., Nakafuku, M., Kaziro, Y., Arai, K. I., Matsumoto, K.
The Saccharomyces cerevisiae GPA1 gene encodes a protein highly homologous to the α subunit of mammalian G proteins and is essential for haploid cell growth. We have selected 77 mutants able to...