Y. Kuchino

Publication List Details

Number

48

Co-Authors

Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: evolutionary implications.

Yokogawa, T, Suzuki, T, Ueda, T, Mori, M, Ohama, T, Kuchino, Y, ...

In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal...

Possible role of splice acceptor site in expression of unspliced gag-containing message of Moloney murine leukemia virus.

Oshima, M, Odawara, T, Matano, T, Sakahira, H, Kuchino, Y, Iwamoto, A, ...

Moloney murine leukemia virus (MLV) having the gag coding region alone, G3.6, produced a low level of mRNA (1/10 of the wild-type level). Ligation of 441 nucleotides (nt) containing a splice acceptor...

A functional role for death proteases in s-Myc- and c-Myc-mediated apoptosis.

Kagaya, S, Kitanaka, C, Noguchi, K, Mochizuki, T, Sugiyama, A, Asai, A, ...

Upon activation, cell surface death receptors, Fas/APO-1/CD95 and tumor necrosis factor receptor-1 (TNFR-1), are attached to cytosolic adaptor proteins, which in turn recruit caspase-8...

Analysis of Moloney murine leukemia virus revertants mutated at the gag-pol junction.

Odawara, T, Yoshikura, H, Ohshima, M, Tanaka, T, Jones, D S, Nemoto, F, ...

Among Moloney murine leukemia viruses (Mo-MuLVs) having stop codons other than UAG at the gag-pol junction, Mo-MuLV with UAA, but not with UGA, had a replication disadvantage. Mo-MuLV with a...

Isolation and characterization of s-myc, a member of the rat myc gene family.

Sugiyama, A, Kume, A, Nemoto, K, Lee, S Y, Asami, Y, Nemoto, F, ...

A 9-kilobase-pair clone containing a myc-related gene that we have designated s-myc was isolated from a rat genomic library. The entire nucleotide sequence of the cloned DNA was determined, showing...

Natural UAG suppressor glutamine tRNA is elevated in mouse cells infected with Moloney murine leukemia virus.

Kuchino, Y, Beier, H, Akita, N, Nishimura, S

Two species of glutamine tRNA were isolated from mouse liver and their nucleotide sequences were determined. The minor glutamine tRNA(tRNA(UmUGGln)) that possesses UmUG (where Um stands for...

The effect of specific mutations at and around the gag-pol gene junction of Moloney murine leukaemia virus.

Jones, D S, Nemoto, F, Kuchino, Y, Masuda, M, Yoshikura, H, Nishimura, S

By carrying out oligonucleotide-directed mutagenesis, in vitro, on a 3.3 kb XhoI-HindIII fragment from Moloney murine leukaemia virus Mo-MuLV proviral DNA, inserted into the phagemid pTZ19R, nine...

Nucleotide sequences of three proline tRNAs from Salmonella typhimurium.

Kuchino, Y, Yabusaki, Y, Mori, F, Nishimura, S

The nucleotide sequences of three proline tRNAs from Salmonella typhimurium were determined by post-labeling procedures. The three proline tRNAs had almost identical sequences in the D-arm and T psi...

Nucleotide sequence of initiator tRNA from Mycobacterium smegmatis.

Vani, B R, Ramakrishnan, T, Kuchino, Y, Nishimura, S

The nucleotide sequence of initiator tRNA from Mycobacterium smegmatis was determined to be pCGCGGGGUGGAGCAGCUCGGDAGCUCGCUGGGCUCAUAACCCAGAGm7GUCG CAGGU psi CGm1AAUCCUGUCCCCGCUACCAOH . The nucleotide...

Mammalian tRNA genes: nucleotide sequence of rat genes for tRNAAsp, tRNAGly and tRNAGlu.

Sekiya, T, Kuchino, Y, Nishimura, S

A cloned 2.1 kb fragment of rat DNA hybridized to purified tRNAAsp has been sequenced. The result revealed that in addition to the putative gene for tRNAAspGAU(C), the fragment contained the...

Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.

Kuchino, Y, Borek, E, Grunberger, D, Mushinski, J F, Nishimura, S

Nucleotide sequences of normal mouse liver tRNAPhe and tumor-specific tRNAPhes isolated from Ehrlich ascites tumor and neuroblastoma cells were examined by post-labeling techniques. The results...

Nucleotide sequence of Streptomyces griseus initiator tRNA.

Kuchino, Y, Yamamoto, I, Nishimura, S

The primary structure of initiator tRNA from Streptomyces griseus was determined by post-labeling procedures. The nucleotide sequence is...

Nucleotide sequence of cytoplasmic initiator tRNA from Tetrahymena thermophila.

Kuchino, Y, Mita, T, Nishimura, S

The total primary structure of cytoplasmic initiator tRNA from Tetrahymena thermophila mating type IV, was determined by post labeling techniques. The sequence is...

Isolation of hydroxy-Y base from rat liver tRNAPhe.

Kasai, H, Yamaizumi, Z, Kuchino, Y, Nishimura, S

A Y-base derivative was isolated from rat liver tRNAPhe and its structure was assigned to be alpha-(carboxyamino)-beta-hydroxy-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-a]purine-7-butyric acid...

Nucleotide sequence of starfish initiator tRNA.

Kuchino, Y, Kato, M, Sugisaki, H, Nishimura, S

The nucleotide sequence of starfish ovary initiator tRNA was determined to be...

Two nucleotides next to the anticodon of cytoplasmic rat tRNA(Asp) are likely generated by RNA editing.

Beier, H, Lee, M C, Sekiya, T, Kuchino, Y, Nishimura, S

The nucleotide sequences of major cytoplasmic tRNA(Asp) from rat liver and rat ascites hepatoma comprise a U32 and C33 next to the anticodon as was confirmed by different procedures. Additionally we...

Identification and nucleotide sequence determination of a potential primer tRNA for reverse transcription of a Drosophila retrotransposon, 297.

Inouye, S, Saigo, K, Yamada, K, Kuchino, Y

Drosophila retrotransposons have been shown to have genes for enzymes similar to the reverse transcriptase of retroviruse. They may possibly be involved in genome replication on translocation (15)....

Structure and transcription of the tRNAPro1 gene from Escherichia coli.

Kuchino, Y, Mori, F, Nishimura, S

A 5 kbp DNA fragment containing the tRNAPro1 gene from Escherichia coli was cloned into Charon 21A phage and sequenced by the M13 DNA sequencing technique. When the cloned DNA fragment was used as a...

Biosynthesis of the modified nucleoside Q in transfer RNA.

Kuchino, Y, Kasai, H, Nihei, K, Nishimura, S

During biosynthesis of the modified nucleoside Q, 7-(4,5-DIHYDROXYL-1-1-CYCLOPENTEN-3-YL-AMINOMETHYL)-7-DEAZAGUANOSINE, IN TRNA, the carbon atom at position 8 in precursor molecule guanine was...

Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's.

Kasai, H, Kuchino, Y, Nihei, K, Nishimura, S

The modified nucleoside, 7-(4,5-cis-dihydroxy-1-cyclopenten-3-yl-aminomethyl)-7-deazaguanosine, designated as Q, and its derivative, Q*, were found in tRNA's from various organisms, including several...

Tetrahymena thermophila glutamine tRNA and its gene that corresponds to UAA termination codon.

Kuchino, Y, Hanyu, N, Tashiro, F, Nishimura, S

Nucleotide sequence analysis of one of several tRNA genes cloned from Tetrahymena thermophila macronuclear DNA indicated that it corresponds to a tRNA species having TTA as anticodon. Subsequently,...

Insertion (sufB) in the anticodon loop or base substitution (sufC) in the anticodon stem of tRNA(Pro)2 from Salmonella typhimurium induces suppression of frameshift mutations.

Sroga, G E, Nemoto, F, Kuchino, Y, Björk, G R

The dominant +1 frameshift suppressors sufA6, sufB1 and sufB2, in Salmonella typhimurium act at runs of C and affect tRNA(Pro)1, tRNA(Pro)2 and tRNA(Pro)2, respectively. A recessive +1 frameshift...

Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: evolutionary implications.

Yokogawa, T, Suzuki, T, Ueda, T, Mori, M, Ohama, T, Kuchino, Y, ...

In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal...

Possible role of splice acceptor site in expression of unspliced gag-containing message of Moloney murine leukemia virus.

Oshima, M, Odawara, T, Matano, T, Sakahira, H, Kuchino, Y, Iwamoto, A, ...

Moloney murine leukemia virus (MLV) having the gag coding region alone, G3.6, produced a low level of mRNA (1/10 of the wild-type level). Ligation of 441 nucleotides (nt) containing a splice acceptor...

A functional role for death proteases in s-Myc- and c-Myc-mediated apoptosis.

Kagaya, S, Kitanaka, C, Noguchi, K, Mochizuki, T, Sugiyama, A, Asai, A, ...

Upon activation, cell surface death receptors, Fas/APO-1/CD95 and tumor necrosis factor receptor-1 (TNFR-1), are attached to cytosolic adaptor proteins, which in turn recruit caspase-8...

Analysis of Moloney murine leukemia virus revertants mutated at the gag-pol junction.

Odawara, T, Yoshikura, H, Ohshima, M, Tanaka, T, Jones, D S, Nemoto, F, ...

Among Moloney murine leukemia viruses (Mo-MuLVs) having stop codons other than UAG at the gag-pol junction, Mo-MuLV with UAA, but not with UGA, had a replication disadvantage. Mo-MuLV with a...

Isolation and characterization of s-myc, a member of the rat myc gene family.

Sugiyama, A, Kume, A, Nemoto, K, Lee, S Y, Asami, Y, Nemoto, F, ...

A 9-kilobase-pair clone containing a myc-related gene that we have designated s-myc was isolated from a rat genomic library. The entire nucleotide sequence of the cloned DNA was determined, showing...

Natural UAG suppressor glutamine tRNA is elevated in mouse cells infected with Moloney murine leukemia virus.

Kuchino, Y, Beier, H, Akita, N, Nishimura, S

Two species of glutamine tRNA were isolated from mouse liver and their nucleotide sequences were determined. The minor glutamine tRNA(tRNA(UmUGGln)) that possesses UmUG (where Um stands for...

Insertion (sufB) in the anticodon loop or base substitution (sufC) in the anticodon stem of tRNA(Pro)2 from Salmonella typhimurium induces suppression of frameshift mutations.

Sroga, G E, Nemoto, F, Kuchino, Y, Björk, G R

The dominant +1 frameshift suppressors sufA6, sufB1 and sufB2, in Salmonella typhimurium act at runs of C and affect tRNA(Pro)1, tRNA(Pro)2 and tRNA(Pro)2, respectively. A recessive +1 frameshift...

The effect of specific mutations at and around the gag-pol gene junction of Moloney murine leukaemia virus.

Jones, D S, Nemoto, F, Kuchino, Y, Masuda, M, Yoshikura, H, Nishimura, S

By carrying out oligonucleotide-directed mutagenesis, in vitro, on a 3.3 kb XhoI-HindIII fragment from Moloney murine leukaemia virus Mo-MuLV proviral DNA, inserted into the phagemid pTZ19R, nine...

Nucleotide sequences of three proline tRNAs from Salmonella typhimurium.

Kuchino, Y, Yabusaki, Y, Mori, F, Nishimura, S

The nucleotide sequences of three proline tRNAs from Salmonella typhimurium were determined by post-labeling procedures. The three proline tRNAs had almost identical sequences in the D-arm and T psi...

Nucleotide sequence of initiator tRNA from Mycobacterium smegmatis.

Vani, B R, Ramakrishnan, T, Kuchino, Y, Nishimura, S

The nucleotide sequence of initiator tRNA from Mycobacterium smegmatis was determined to be pCGCGGGGUGGAGCAGCUCGGDAGCUCGCUGGGCUCAUAACCCAGAGm7GUCG CAGGU psi CGm1AAUCCUGUCCCCGCUACCAOH . The nucleotide...

Mammalian tRNA genes: nucleotide sequence of rat genes for tRNAAsp, tRNAGly and tRNAGlu.

Sekiya, T, Kuchino, Y, Nishimura, S

A cloned 2.1 kb fragment of rat DNA hybridized to purified tRNAAsp has been sequenced. The result revealed that in addition to the putative gene for tRNAAspGAU(C), the fragment contained the...

Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.

Kuchino, Y, Borek, E, Grunberger, D, Mushinski, J F, Nishimura, S

Nucleotide sequences of normal mouse liver tRNAPhe and tumor-specific tRNAPhes isolated from Ehrlich ascites tumor and neuroblastoma cells were examined by post-labeling techniques. The results...

Nucleotide sequence of Streptomyces griseus initiator tRNA.

Kuchino, Y, Yamamoto, I, Nishimura, S

The primary structure of initiator tRNA from Streptomyces griseus was determined by post-labeling procedures. The nucleotide sequence is...

Nucleotide sequence of cytoplasmic initiator tRNA from Tetrahymena thermophila.

Kuchino, Y, Mita, T, Nishimura, S

The total primary structure of cytoplasmic initiator tRNA from Tetrahymena thermophila mating type IV, was determined by post labeling techniques. The sequence is...

Isolation of hydroxy-Y base from rat liver tRNAPhe.

Kasai, H, Yamaizumi, Z, Kuchino, Y, Nishimura, S

A Y-base derivative was isolated from rat liver tRNAPhe and its structure was assigned to be alpha-(carboxyamino)-beta-hydroxy-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-a]purine-7-butyric acid...

Nucleotide sequence of starfish initiator tRNA.

Kuchino, Y, Kato, M, Sugisaki, H, Nishimura, S

The nucleotide sequence of starfish ovary initiator tRNA was determined to be...

Two nucleotides next to the anticodon of cytoplasmic rat tRNA(Asp) are likely generated by RNA editing.

Beier, H, Lee, M C, Sekiya, T, Kuchino, Y, Nishimura, S

The nucleotide sequences of major cytoplasmic tRNA(Asp) from rat liver and rat ascites hepatoma comprise a U32 and C33 next to the anticodon as was confirmed by different procedures. Additionally we...

Identification and nucleotide sequence determination of a potential primer tRNA for reverse transcription of a Drosophila retrotransposon, 297.

Inouye, S, Saigo, K, Yamada, K, Kuchino, Y

Drosophila retrotransposons have been shown to have genes for enzymes similar to the reverse transcriptase of retroviruse. They may possibly be involved in genome replication on translocation (15)....

Structure and transcription of the tRNAPro1 gene from Escherichia coli.

Kuchino, Y, Mori, F, Nishimura, S

A 5 kbp DNA fragment containing the tRNAPro1 gene from Escherichia coli was cloned into Charon 21A phage and sequenced by the M13 DNA sequencing technique. When the cloned DNA fragment was used as a...

Biosynthesis of the modified nucleoside Q in transfer RNA.

Kuchino, Y, Kasai, H, Nihei, K, Nishimura, S

During biosynthesis of the modified nucleoside Q, 7-(4,5-DIHYDROXYL-1-1-CYCLOPENTEN-3-YL-AMINOMETHYL)-7-DEAZAGUANOSINE, IN TRNA, the carbon atom at position 8 in precursor molecule guanine was...

Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's.

Kasai, H, Kuchino, Y, Nihei, K, Nishimura, S

The modified nucleoside, 7-(4,5-cis-dihydroxy-1-cyclopenten-3-yl-aminomethyl)-7-deazaguanosine, designated as Q, and its derivative, Q*, were found in tRNA's from various organisms, including several...

Tetrahymena thermophila glutamine tRNA and its gene that corresponds to UAA termination codon.

Kuchino, Y, Hanyu, N, Tashiro, F, Nishimura, S

Nucleotide sequence analysis of one of several tRNA genes cloned from Tetrahymena thermophila macronuclear DNA indicated that it corresponds to a tRNA species having TTA as anticodon. Subsequently,...