Y. Miyakawa

Publication List Details

Period

1987 - 2005

Number

54

Co-Authors

Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene (2002)

Sugauchi, F, Orito, E, Ichida, T, Kato, H, Sakugawa, H, Kakumu, S, ...

The entire nucleotide sequences of 70 hepatitis B virus (HBV) isolates of genotype B (HBV/B), including 38 newly determined and 32 retrieved from the international DNA database (DDBJ/EMBL/GenBank),...

Mammary and renal tumor induction by low doses of adriamycin in Sprague-Dawley rats (1987)

Jang, J.J., Takahashi, M., Hasegawa, R., Furukawa, F., Toyoda, K., Sato, H., ...

The oncogenic potential of adriamycin was studied in both sexes of Spragu-Dawley rats. Single i.v. injection of 10 or 2 mg and repeated injections of 2 mg of adriamycin/kg body wt within 2 weeks...

Hepatitis C virus variants from Vietnam are classifiable into the seventh, eighth, and ninth major genetic groups.

Tokita, H, Okamoto, H, Tsuda, F, Song, P, Nakata, S, Chosa, T, ...

Thirty-four (41%) of 83 hepatitis C virus (HCV) isolates from commercial blood donors in Vietnam were not classifiable into genotype I/1a, II/1b, III/2a, IV/2b, or V/3a; for 15 of them, the sequence...

In vitro activity of the antimicrobial peptides human and rabbit defensins and porcine leukocyte protegrin against Mycobacterium tuberculosis.

Miyakawa, Y, Ratnakar, P, Rao, A G, Costello, M L, Mathieu-Costello, O, Lehrer, R I, ...

Three independent assay methods were used to investigate the activities of antimicrobial peptides (human and rabbit defensins and protegrin from porcine leukocytes) against Mycobacterium tuberculosis...

Timed action of the gene products required for septum formation in the cell cycle of Bacillus subtilis.

Miyakawa, Y, Komano, T, Maruyama, Y

Four isogenic strains of temperature-sensitive septationless mutants, whose mutations are located on different genes, were used to study the periods of action of the gene products required for the...

Naturally occurring escape mutants of hepatitis B virus with various mutations in the S gene in carriers seropositive for antibody to hepatitis B surface antigen.

Yamamoto, K, Horikita, M, Tsuda, F, Itoh, K, Akahane, Y, Yotsumoto, S, ...

Hepatitis B virus (HBV) DNA was extracted from sera of six carriers with hepatitis B e antigen as well as antibody to hepatitis B surface antigen and sequenced within the pre-S regions and the S...

Hepatitis B virus with mutations in the core promoter for an e antigen-negative phenotype in carriers with antibody to e antigen.

Okamoto, H, Tsuda, F, Akahane, Y, Sugai, Y, Yoshiba, M, Moriyama, K, ...

Hepatitis B virus (HBV) DNA clones were propagated from 57 carriers with antibody to hepatitis B e antigen (HBeAg) and sequenced within nucleotides (nt) 1685 to 1926 including the core promoter (nt...

Allelic subtypic determinants of hepatitis B surface antigen (i and t) that are distinct from d/y or w/r.

Ohnuma, H, Machida, A, Okamoto, H, Tsuda, F, Sakamoto, M, Tanaka, T, ...

A monoclonal antibody (I-18) was raised against an enneapeptide representing amino acids 125 to 133 of the product of the S gene of hepatitis B virus DNA [S(125-133) segment] with a sequence of...

Chimeric hepatitis B virus core particles with parts or copies of the hepatitis C virus core protein.

Yoshikawa, A, Tanaka, T, Hoshi, Y, Kato, N, Tachibana, K, Iizuka, H, ...

Either parts or multiple copies of the core gene of hepatitis C virus (HCV) were fused to the 3' terminus of the hepatitis B virus (HBV) core gene with 34 codons removed. As many as four copies of...

Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen.

Okamoto, H, Yotsumoto, S, Akahane, Y, Yamanaka, T, Miyazaki, Y, Sugai, Y, ...

The C gene of hepatitis B virus (HBV) codes for a nucleocapsid protein made of 183 amino acid residues and is preceded in phase by the precore (pre-C) region, encoding 29 residues. The pre-C-region...

Phosphorylation in the carboxyl-terminal domain of the capsid protein of hepatitis B virus: evaluation with a monoclonal antibody.

Machida, A, Ohnuma, H, Tsuda, F, Yoshikawa, A, Hoshi, Y, Tanaka, T, ...

The capsid protein of hepatitis B virus (p21c) is made of 183 amino acids coded for by the C gene. By using p21c isolated from Dane particles (hepatitis B virus) as an immunogen, a monoclonal...

Point mutation in the S gene of hepatitis B virus for a d/y or w/r subtypic change in two blood donors carrying a surface antigen of compound subtype adyr or adwr.

Okamoto, H, Imai, M, Tsuda, F, Tanaka, T, Miyakawa, Y, Mayumi, M

Genomes of hepatitis B virus (HBV) were cloned from the plasma of a blood donor who carried subviral particles of three distinct subtypes in the following proportions: adr, 25%; ayr, 63%; and adyr,...

Free and integrated forms of hepatitis B virus DNA in human hepatocellular carcinoma cells (PLC/342) propagated in nude mice.

Imai, M, Hoshi, Y, Okamoto, H, Matsui, T, Tsurimoto, T, Matsubara, K, ...

Primary hepatocellular carcinoma cells (PLC/342) propagated in nude mice produce hepatitis B surface antigen of subtype adr, as well as core particles containing viral DNA and DNA polymerase. Free...

Antigenic role of stress-induced catalase of Salmonella typhimurium in cell-mediated immunity.

Kagaya, K, Miyakawa, Y, Watanabe, K, Fukazawa, Y

The ability of the H2O2-induced catalase of Salmonella typhimurium to induce cell-mediated immunity against S. typhimurium infection in mice was examined. When exponentially growing cells of S....

Role of specific determinants in mannan of Candida albicans serotype A in adherence to human buccal epithelial cells.

Miyakawa, Y, Kuribayashi, T, Kagaya, K, Suzuki, M, Nakase, T, Fukazawa, Y

Candida albicans serotype A (C. albicans A) possesses a specific antigen, designated antigen 6, which resides in mannans on the cell surface. To determine the role of the mannan moiety of the C....

Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction.

Miyakawa, Y, Mabuchi, T, Kagaya, K, Fukazawa, Y

A 2-kbp DNA fragment, EO3, that was present in multiple copies in the Candida albicans genome was isolated for use in developing a detection method for C. albicans by polymerase chain reaction (PCR)....

New method for detection of Candida albicans in human blood by polymerase chain reaction.

Miyakawa, Y, Mabuchi, T, Fukazawa, Y

We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment....

Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans.

Miyakawa, Y, Kagaya, K, Fukazawa, Y, Soe, G

Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity...

Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein.

Nagayama, R, Tsuda, F, Okamoto, H, Wang, Y, Mitsui, T, Tanaka, T, ...

Hepatitis C virus (HCV) samples in 155 sera, from patients with chronic non-A, non-B liver disease and blood donors, were grouped into four genotypes (I, II, III, and IV) by amplification of...

Cell-cycle-specific inhibition by chloramphenicol of septum fromation and cell division in synchronized cells of Bacillus subtilis.

Miyakawa, Y, Komano, T, Maruyama, Y

The relationship between protein synthesis and processes of cell division was studied by using synchronized cells of Bacillus subtilis 168. The addition of chloramphenicol at the beginning of...

A synthetic peptide vaccine involving the product of the pre-S(2) region of hepatitis B virus DNA: protective efficacy in chimpanzees.

Itoh, Y, Takai, E, Ohnuma, H, Kitajima, K, Tsuda, F, Machida, A, ...

The S gene encoding the major surface polypeptide of hepatitis B virus is preceded by the region pre-S(2) with a capacity to code for 55 amino acid residues. In the product of region pre-S(2), the...

Duality of hepatitis B e antigen in serum of persons infected with hepatitis B virus: evidence for the nonidentity of e antigen with immunoglobulins.

Takahashi, K, Imai, M, Miyakawa, Y, Iwakiri, S, Mayumi, M

Hepatitis B e antigen (HBeAg) is detected in the serum of some persons infected with hepatitis B virus. Owing to a close correlation of HBeAg and hepatitis B virus in the serum, it has been used as a...

Hepatitis B antigen-associated deoxyribonucleic acid polymerase activity and e antigen/anti-e system.

Imai, M, Tachibana, F C, Moritsugu, Y, Miyakawa, Y, Mayumi, M

Serum samples of 403 asymptomatic blood donors carrying hepatitis B surface antigen (HB5Ag) were concentrated threefold and tested for e antigen and antibody to e antigen (anti-e) by immunodiffusion....

Hepatitis C virus variants from Vietnam are classifiable into the seventh, eighth, and ninth major genetic groups.

Tokita, H, Okamoto, H, Tsuda, F, Song, P, Nakata, S, Chosa, T, ...

Thirty-four (41%) of 83 hepatitis C virus (HCV) isolates from commercial blood donors in Vietnam were not classifiable into genotype I/1a, II/1b, III/2a, IV/2b, or V/3a; for 15 of them, the sequence...

In vitro activity of the antimicrobial peptides human and rabbit defensins and porcine leukocyte protegrin against Mycobacterium tuberculosis.

Miyakawa, Y, Ratnakar, P, Rao, A G, Costello, M L, Mathieu-Costello, O, Lehrer, R I, ...

Three independent assay methods were used to investigate the activities of antimicrobial peptides (human and rabbit defensins and protegrin from porcine leukocytes) against Mycobacterium tuberculosis...

Timed action of the gene products required for septum formation in the cell cycle of Bacillus subtilis.

Miyakawa, Y, Komano, T, Maruyama, Y

Four isogenic strains of temperature-sensitive septationless mutants, whose mutations are located on different genes, were used to study the periods of action of the gene products required for the...

Naturally occurring escape mutants of hepatitis B virus with various mutations in the S gene in carriers seropositive for antibody to hepatitis B surface antigen.

Yamamoto, K, Horikita, M, Tsuda, F, Itoh, K, Akahane, Y, Yotsumoto, S, ...

Hepatitis B virus (HBV) DNA was extracted from sera of six carriers with hepatitis B e antigen as well as antibody to hepatitis B surface antigen and sequenced within the pre-S regions and the S...

Hepatitis B virus with mutations in the core promoter for an e antigen-negative phenotype in carriers with antibody to e antigen.

Okamoto, H, Tsuda, F, Akahane, Y, Sugai, Y, Yoshiba, M, Moriyama, K, ...

Hepatitis B virus (HBV) DNA clones were propagated from 57 carriers with antibody to hepatitis B e antigen (HBeAg) and sequenced within nucleotides (nt) 1685 to 1926 including the core promoter (nt...

Allelic subtypic determinants of hepatitis B surface antigen (i and t) that are distinct from d/y or w/r.

Ohnuma, H, Machida, A, Okamoto, H, Tsuda, F, Sakamoto, M, Tanaka, T, ...

A monoclonal antibody (I-18) was raised against an enneapeptide representing amino acids 125 to 133 of the product of the S gene of hepatitis B virus DNA [S(125-133) segment] with a sequence of...

Chimeric hepatitis B virus core particles with parts or copies of the hepatitis C virus core protein.

Yoshikawa, A, Tanaka, T, Hoshi, Y, Kato, N, Tachibana, K, Iizuka, H, ...

Either parts or multiple copies of the core gene of hepatitis C virus (HCV) were fused to the 3' terminus of the hepatitis B virus (HBV) core gene with 34 codons removed. As many as four copies of...

Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen.

Okamoto, H, Yotsumoto, S, Akahane, Y, Yamanaka, T, Miyazaki, Y, Sugai, Y, ...

The C gene of hepatitis B virus (HBV) codes for a nucleocapsid protein made of 183 amino acid residues and is preceded in phase by the precore (pre-C) region, encoding 29 residues. The pre-C-region...

Phosphorylation in the carboxyl-terminal domain of the capsid protein of hepatitis B virus: evaluation with a monoclonal antibody.

Machida, A, Ohnuma, H, Tsuda, F, Yoshikawa, A, Hoshi, Y, Tanaka, T, ...

The capsid protein of hepatitis B virus (p21c) is made of 183 amino acids coded for by the C gene. By using p21c isolated from Dane particles (hepatitis B virus) as an immunogen, a monoclonal...

Point mutation in the S gene of hepatitis B virus for a d/y or w/r subtypic change in two blood donors carrying a surface antigen of compound subtype adyr or adwr.

Okamoto, H, Imai, M, Tsuda, F, Tanaka, T, Miyakawa, Y, Mayumi, M

Genomes of hepatitis B virus (HBV) were cloned from the plasma of a blood donor who carried subviral particles of three distinct subtypes in the following proportions: adr, 25%; ayr, 63%; and adyr,...

Free and integrated forms of hepatitis B virus DNA in human hepatocellular carcinoma cells (PLC/342) propagated in nude mice.

Imai, M, Hoshi, Y, Okamoto, H, Matsui, T, Tsurimoto, T, Matsubara, K, ...

Primary hepatocellular carcinoma cells (PLC/342) propagated in nude mice produce hepatitis B surface antigen of subtype adr, as well as core particles containing viral DNA and DNA polymerase. Free...

Antigenic role of stress-induced catalase of Salmonella typhimurium in cell-mediated immunity.

Kagaya, K, Miyakawa, Y, Watanabe, K, Fukazawa, Y

The ability of the H2O2-induced catalase of Salmonella typhimurium to induce cell-mediated immunity against S. typhimurium infection in mice was examined. When exponentially growing cells of S....

Role of specific determinants in mannan of Candida albicans serotype A in adherence to human buccal epithelial cells.

Miyakawa, Y, Kuribayashi, T, Kagaya, K, Suzuki, M, Nakase, T, Fukazawa, Y

Candida albicans serotype A (C. albicans A) possesses a specific antigen, designated antigen 6, which resides in mannans on the cell surface. To determine the role of the mannan moiety of the C....

Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction.

Miyakawa, Y, Mabuchi, T, Kagaya, K, Fukazawa, Y

A 2-kbp DNA fragment, EO3, that was present in multiple copies in the Candida albicans genome was isolated for use in developing a detection method for C. albicans by polymerase chain reaction (PCR)....

New method for detection of Candida albicans in human blood by polymerase chain reaction.

Miyakawa, Y, Mabuchi, T, Fukazawa, Y

We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment....

Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans.

Miyakawa, Y, Kagaya, K, Fukazawa, Y, Soe, G

Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity...

Genotype dependence of hepatitis C virus antibodies detectable by the first-generation enzyme-linked immunosorbent assay with C100-3 protein.

Nagayama, R, Tsuda, F, Okamoto, H, Wang, Y, Mitsui, T, Tanaka, T, ...

Hepatitis C virus (HCV) samples in 155 sera, from patients with chronic non-A, non-B liver disease and blood donors, were grouped into four genotypes (I, II, III, and IV) by amplification of...

Cell-cycle-specific inhibition by chloramphenicol of septum fromation and cell division in synchronized cells of Bacillus subtilis.

Miyakawa, Y, Komano, T, Maruyama, Y

The relationship between protein synthesis and processes of cell division was studied by using synchronized cells of Bacillus subtilis 168. The addition of chloramphenicol at the beginning of...

A synthetic peptide vaccine involving the product of the pre-S(2) region of hepatitis B virus DNA: protective efficacy in chimpanzees.

Itoh, Y, Takai, E, Ohnuma, H, Kitajima, K, Tsuda, F, Machida, A, ...

The S gene encoding the major surface polypeptide of hepatitis B virus is preceded by the region pre-S(2) with a capacity to code for 55 amino acid residues. In the product of region pre-S(2), the...

Duality of hepatitis B e antigen in serum of persons infected with hepatitis B virus: evidence for the nonidentity of e antigen with immunoglobulins.

Takahashi, K, Imai, M, Miyakawa, Y, Iwakiri, S, Mayumi, M

Hepatitis B e antigen (HBeAg) is detected in the serum of some persons infected with hepatitis B virus. Owing to a close correlation of HBeAg and hepatitis B virus in the serum, it has been used as a...

Hepatitis B antigen-associated deoxyribonucleic acid polymerase activity and e antigen/anti-e system.

Imai, M, Tachibana, F C, Moritsugu, Y, Miyakawa, Y, Mayumi, M

Serum samples of 403 asymptomatic blood donors carrying hepatitis B surface antigen (HB5Ag) were concentrated threefold and tested for e antigen and antibody to e antigen (anti-e) by immunodiffusion....

Common antigen structures of HL-A antigens: I. Antigenic determinants recognizable by rabbits on papain- solubilized HL-A molecular fragments

Miyakawa, Y., Tanigaki, N., Yagi, Y., Pressman, D.

Antigenic determinants recognizable by rabbits were found to be present on the molecular fragments (48,000 Daltons) which were obtained by papain-solubilization of the membrane fractions of cultured...

Haemagglutination inhibition assay of the common determinants and subspecificities of Australia antigen

Imai, M., Yamashita, Y., Miyakawa, Y., Mayumi, M.

Antigenic determinants of Australia antigen (AuAg) may be determined by haemagglutination inhibition with ease and high sensitivity. The reaction depends on the inhibitory effect of the test...

Application of human erythrocytes to a radioimmune assay of immune complexes in serum

Tsuda, F., Miyakawa, Y., Mayumi, M.

An immune adherence receptor exists on the surface of primate erythrocytes, and has been characterized as a receptor for the activated third component of complement (C3b). We have applied human red...

Small and large forms of hepatitis B e antigen in the serum: determination by two-site sandwich radioimmunoassay with monoclonal antibodies.

Baba, K, Ise, I, Aihara, S, Kishimoto, S, Tsuda, F, Tachibana, K, ...

Serum samples containing hepatitis B e antigen (HBeAg) were subjected to electrophoresis in agarose, and fast-migrating 'small' HBeAg and slow-migrating 'large' IgG-associated HBeAg were pooled...

Translation products of pre-S(1), pre-S(2) regions and the S gene of hepatitis B virus: susceptibility of their antigenic activities to treatment with heat, urea formalin or pepsin

Ohnuma, H., Machida, A., Takai, Emiko, Tsuda, F., Miyamoto, H., Tanaka, T., ...

Hepatitis B subviral particles, purified from plasma of asymptomatic carriers seropositive for hepatitis B e antigen, were treated with various conditions reported for the processing of vaccines....

Influence of cerebral embolism on brain monoamines

Ishihara, N., Welch, K. M. A., Meyer, J. S., Chabi, E., Naritomi, H., Wang, T-P. F., ...

In baboons the right cerebral hemisphere was embolised by a shower of microemboli, immediately followed by one large embolus designed to occlude the middle cerebral artery (MCA). One hour after...