Taxonomy and function of C1 protein kinase C homology domains (1997)
HURLEY, J. H., NEWTON, A. C., PARKER, P. J., BLUMBERG, P. M., NISHIZUKA, Y.
Mammalian phospholipase D: phosphatidylethanolamine as an essential component.
Nakamura, S, Kiyohara, Y, Jinnai, H, Hitomi, T, Ogino, C, Yoshida, K, ...
Bovine kidney phospholipase D (PLD) was assayed by measuring the formation of phosphatidylethanol from added radioactive phosphatidylcholine (PtdCho) in the presence of ethanol, guanosine...
Mammalian phospholipase D: activation by ammonium sulfate and nucleotides.
Nakamura, S, Shimooku, K, Akisue, T, Jinnai, H, Hitomi, T, Kiyohara, Y, ...
Phospholipase D (PLD) associated with the rat kidney membrane was activated by guanine 5'-[gamma-thio]triphosphate and a cytosol fraction that contained ADP-ribosylation factor. When assayed by...
Tsujishita, Y, Asaoka, Y, Nishizuka, Y
Permeabilized human leukemia HL-60 and U-937 cells suspended in an acidic or alkaline medium release various unsaturated fatty acids, most abundantly oleic and arachidonic acids. Concomitant...
Asaoka, Y, Yoshida, K, Sasaki, Y, Nishizuka, Y, Murakami, M, Kudo, I, ...
Both 2-lysophosphatidylcholine and cis-unsaturated fatty acids were previously shown to intensify agonist-induced cellular responses by enhancing the diacylglycerol-dependent activation of protein...
Asaoka, Y, Yoshida, K, Sasaki, Y, Nishizuka, Y
2-Lysophosphatidylcholine and cis-unsaturated fatty acids such as linoleic and linolenic acids, which are the products of the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2 (EC...
Isolation and characterization of the epsilon subspecies of protein kinase C from rat brain.
Koide, H, Ogita, K, Kikkawa, U, Nishizuka, Y
The epsilon subspecies of protein kinase C (epsilon PKC) was purified to near homogeneity from the soluble fraction of rat brain by successive chromatographies on DEAE-cellulose, threonine-Sepharose,...
Isolation and characterization of delta-subspecies of protein kinase C from rat brain.
Ogita, K, Miyamoto, S, Yamaguchi, K, Koide, H, Fujisawa, N, Kikkawa, U, ...
The delta-subspecies of protein kinase C (delta PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose...
Platelet activation by simultaneous actions of diacylglycerol and unsaturated fatty acids.
Yoshida, K, Asaoka, Y, Nishizuka, Y
Several cis-unsaturated fatty acids such as oleic, linoleic, linolenic, eicosapentaenoic, and docosahexaenoic acids added directly to intact human platelets greatly enhance protein kinase C...
Asaoka, Y, Oka, M, Yoshida, K, Sasaki, Y, Nishizuka, Y
2-Lysophosphatidylcholine (lysoPtdCho), a product of the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2, greatly potentiates the activation of human resting T lymphocytes that is...
Watanabe, T, Ono, Y, Taniyama, Y, Hazama, K, Igarashi, K, Ogita, K, ...
Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate...
Shinomura, T, Asaoka, Y, Oka, M, Yoshida, K, Nishizuka, Y
Kinetic properties of the purified alpha, beta, and gamma subspecies of protein kinase C (PKC) to respond to diacylglycerol, phosphatidylserine (PtdSer), and Ca2+ were reinvestigated in the presence...
Asaoka, Y, Oka, M, Yoshida, K, Nishizuka, Y
DL-1,2-Dioctanoylglycerol (1,2-DiC8) added to human peripheral resting T lymphocytes was rapidly metabolized to produce octanoic acid and further to small molecules, probably by the action of...
Sustained activation of protein kinase C is essential to HL-60 cell differentiation to macrophage.
Aihara, H, Asaoka, Y, Yoshida, K, Nishizuka, Y
Although a single dose of phorbol 12-myristate 13-acetate (PMA) allowed HL-60 cells to differentiate to macrophages, a single dose of membrane-permeant diacylglycerol (DAG), 1,2-dioctanoylglycerol...
Activation of resting human T cells requires prolonged stimulation of protein kinase C.
Berry, N, Ase, K, Kishimoto, A, Nishizuka, Y
Purified resting human T cells can be induced to express the alpha subunit of the interleukin 2 receptor and to proliferate by treatment with 12-O-tetradecanoylphorbol-13-acetate plus ionomycin but...
Immunocytochemical localization of the alpha subspecies of protein kinase C in rat brain.
Ito, A, Saito, N, Hirata, M, Kose, A, Tsujino, T, Yoshihara, C, ...
The distribution of the alpha subspecies of protein kinase C (PKC) in rat brain was demonstrated immunocytochemically by using polyclonal antibodies raised against a synthetic oligopeptide...
Protein kinase C in Saccharomyces cerevisiae: comparison with the mammalian enzyme.
Ogita, K, Miyamoto, S, Koide, H, Iwai, T, Oka, M, Ando, K, ...
Protein kinase C (PKC) was detected in the yeast Saccharomyces cerevisiae with bovine myelin basic protein as the phosphate acceptor. The enzyme was purified at least 500-fold by a four-step column...
Comparison of guanosine triphosphate split and polypeptide synthesis with a purified E. coli system.
Immunocytochemical localization of the beta I subspecies of protein kinase C in rat brain.
Hosoda, K, Saito, N, Kose, A, Ito, A, Tsujino, T, Ogita, K, ...
Polyclonal antibodies were raised against an oligopeptide containing an amino acid sequence specific for beta I protein kinase C (PKC) in order to localize this subspecies of PKC in the rat brain....
Protein kinase C zeta subspecies from rat brain: its structure, expression, and properties.
Ono, Y, Fujii, T, Ogita, K, Kikkawa, U, Igarashi, K, Nishizuka, Y
The primary structure of the zeta subspecies of rat brain protein kinase C was deduced from its overlapping cDNAs. The zeta subspecies of protein kinase C consists of 592 amino acid residues with the...
Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence.
Ono, Y, Fujii, T, Igarashi, K, Kuno, T, Tanaka, C, Kikkawa, U, ...
Protein kinase C normally has a tandem repeat of a characteristic cysteine-rich sequence in C1, the conserved region of the regulatory domain. These sequences resemble the DNA-binding zinc finger...
Hamasima, N, Takahashi, T, Taguchi, O, Nishizuka, Y, Stockert, E, Old, L J, ...
To investigate the genetic regulation of TL expression, 12 transgenic mouse strains on a C3H (TL-nonexpressing) background have been derived: two Tg.Tlaa-3 strains with Tlaa-3 isolated from A-strain...
Nucleotide sequences of cDNAs for alpha and gamma subspecies of rat brain protein kinase C.
Ono, Y, Fujii, T, Igarashi, K, Kikkawa, U, Ogita, K, Nishizuka, Y
Ueda, R, Tanimoto, M, Takahashi, T, Ogata, S, Nishida, K, Namikawa, R, ...
Nine antigens systems were defined. Two were related to HLA-A,B,C and to Ia-like antigens; the others could be grouped into three categories. (i) NL-22, NL-1: NL-22 antibody reacted with leukemia...
Nishida, Y, Hata, M, Ayaki, T, Ryo, H, Yamagata, M, Shimizu, K, ...
The genomic and cDNA fragments of Drosophila melanogaster, homologous to human c-raf-1, were cloned. The nucleotide sequence predicted the primary structure of a polypeptide of 666 amino acid...
Mammalian phospholipase D: phosphatidylethanolamine as an essential component.
Nakamura, S, Kiyohara, Y, Jinnai, H, Hitomi, T, Ogino, C, Yoshida, K, ...
Bovine kidney phospholipase D (PLD) was assayed by measuring the formation of phosphatidylethanol from added radioactive phosphatidylcholine (PtdCho) in the presence of ethanol, guanosine...
Mammalian phospholipase D: activation by ammonium sulfate and nucleotides.
Nakamura, S, Shimooku, K, Akisue, T, Jinnai, H, Hitomi, T, Kiyohara, Y, ...
Phospholipase D (PLD) associated with the rat kidney membrane was activated by guanine 5'-[gamma-thio]triphosphate and a cytosol fraction that contained ADP-ribosylation factor. When assayed by...
Tsujishita, Y, Asaoka, Y, Nishizuka, Y
Permeabilized human leukemia HL-60 and U-937 cells suspended in an acidic or alkaline medium release various unsaturated fatty acids, most abundantly oleic and arachidonic acids. Concomitant...
Asaoka, Y, Yoshida, K, Sasaki, Y, Nishizuka, Y, Murakami, M, Kudo, I, ...
Both 2-lysophosphatidylcholine and cis-unsaturated fatty acids were previously shown to intensify agonist-induced cellular responses by enhancing the diacylglycerol-dependent activation of protein...
Asaoka, Y, Yoshida, K, Sasaki, Y, Nishizuka, Y
2-Lysophosphatidylcholine and cis-unsaturated fatty acids such as linoleic and linolenic acids, which are the products of the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2 (EC...
Isolation and characterization of the epsilon subspecies of protein kinase C from rat brain.
Koide, H, Ogita, K, Kikkawa, U, Nishizuka, Y
The epsilon subspecies of protein kinase C (epsilon PKC) was purified to near homogeneity from the soluble fraction of rat brain by successive chromatographies on DEAE-cellulose, threonine-Sepharose,...
Isolation and characterization of delta-subspecies of protein kinase C from rat brain.
Ogita, K, Miyamoto, S, Yamaguchi, K, Koide, H, Fujisawa, N, Kikkawa, U, ...
The delta-subspecies of protein kinase C (delta PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose...
Platelet activation by simultaneous actions of diacylglycerol and unsaturated fatty acids.
Yoshida, K, Asaoka, Y, Nishizuka, Y
Several cis-unsaturated fatty acids such as oleic, linoleic, linolenic, eicosapentaenoic, and docosahexaenoic acids added directly to intact human platelets greatly enhance protein kinase C...
Asaoka, Y, Oka, M, Yoshida, K, Sasaki, Y, Nishizuka, Y
2-Lysophosphatidylcholine (lysoPtdCho), a product of the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2, greatly potentiates the activation of human resting T lymphocytes that is...
Watanabe, T, Ono, Y, Taniyama, Y, Hazama, K, Igarashi, K, Ogita, K, ...
Several lines of CHO cells stably overexpressing protein kinase C (PKC) subspecies to various extents were established by the DNA-mediated transfer. Upon treatment with phorbol 12-myristate...
Shinomura, T, Asaoka, Y, Oka, M, Yoshida, K, Nishizuka, Y
Kinetic properties of the purified alpha, beta, and gamma subspecies of protein kinase C (PKC) to respond to diacylglycerol, phosphatidylserine (PtdSer), and Ca2+ were reinvestigated in the presence...
Asaoka, Y, Oka, M, Yoshida, K, Nishizuka, Y
DL-1,2-Dioctanoylglycerol (1,2-DiC8) added to human peripheral resting T lymphocytes was rapidly metabolized to produce octanoic acid and further to small molecules, probably by the action of...
Sustained activation of protein kinase C is essential to HL-60 cell differentiation to macrophage.
Aihara, H, Asaoka, Y, Yoshida, K, Nishizuka, Y
Although a single dose of phorbol 12-myristate 13-acetate (PMA) allowed HL-60 cells to differentiate to macrophages, a single dose of membrane-permeant diacylglycerol (DAG), 1,2-dioctanoylglycerol...
Activation of resting human T cells requires prolonged stimulation of protein kinase C.
Berry, N, Ase, K, Kishimoto, A, Nishizuka, Y
Purified resting human T cells can be induced to express the alpha subunit of the interleukin 2 receptor and to proliferate by treatment with 12-O-tetradecanoylphorbol-13-acetate plus ionomycin but...
Immunocytochemical localization of the alpha subspecies of protein kinase C in rat brain.
Ito, A, Saito, N, Hirata, M, Kose, A, Tsujino, T, Yoshihara, C, ...
The distribution of the alpha subspecies of protein kinase C (PKC) in rat brain was demonstrated immunocytochemically by using polyclonal antibodies raised against a synthetic oligopeptide...
Protein kinase C in Saccharomyces cerevisiae: comparison with the mammalian enzyme.
Ogita, K, Miyamoto, S, Koide, H, Iwai, T, Oka, M, Ando, K, ...
Protein kinase C (PKC) was detected in the yeast Saccharomyces cerevisiae with bovine myelin basic protein as the phosphate acceptor. The enzyme was purified at least 500-fold by a four-step column...
Comparison of guanosine triphosphate split and polypeptide synthesis with a purified E. coli system.
Immunocytochemical localization of the beta I subspecies of protein kinase C in rat brain.
Hosoda, K, Saito, N, Kose, A, Ito, A, Tsujino, T, Ogita, K, ...
Polyclonal antibodies were raised against an oligopeptide containing an amino acid sequence specific for beta I protein kinase C (PKC) in order to localize this subspecies of PKC in the rat brain....
Protein kinase C zeta subspecies from rat brain: its structure, expression, and properties.
Ono, Y, Fujii, T, Ogita, K, Kikkawa, U, Igarashi, K, Nishizuka, Y
The primary structure of the zeta subspecies of rat brain protein kinase C was deduced from its overlapping cDNAs. The zeta subspecies of protein kinase C consists of 592 amino acid residues with the...
Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence.
Ono, Y, Fujii, T, Igarashi, K, Kuno, T, Tanaka, C, Kikkawa, U, ...
Protein kinase C normally has a tandem repeat of a characteristic cysteine-rich sequence in C1, the conserved region of the regulatory domain. These sequences resemble the DNA-binding zinc finger...
Hamasima, N, Takahashi, T, Taguchi, O, Nishizuka, Y, Stockert, E, Old, L J, ...
To investigate the genetic regulation of TL expression, 12 transgenic mouse strains on a C3H (TL-nonexpressing) background have been derived: two Tg.Tlaa-3 strains with Tlaa-3 isolated from A-strain...
Nucleotide sequences of cDNAs for alpha and gamma subspecies of rat brain protein kinase C.
Ono, Y, Fujii, T, Igarashi, K, Kikkawa, U, Ogita, K, Nishizuka, Y
Ueda, R, Tanimoto, M, Takahashi, T, Ogata, S, Nishida, K, Namikawa, R, ...
Nine antigens systems were defined. Two were related to HLA-A,B,C and to Ia-like antigens; the others could be grouped into three categories. (i) NL-22, NL-1: NL-22 antibody reacted with leukemia...
Nishida, Y, Hata, M, Ayaki, T, Ryo, H, Yamagata, M, Shimizu, K, ...
The genomic and cDNA fragments of Drosophila melanogaster, homologous to human c-raf-1, were cloned. The nucleotide sequence predicted the primary structure of a polypeptide of 666 amino acid...
Experimental autoimmune orchitis after neonatal thymectomy in the mouse.
Experimental autoimmune orchitis (EAO) developed in (C57Bl/6Cr x A/JCr)F1 mice 3-4 months after neonatal thymectomy (Tx) without any sensitization. The age of Tx was critical for induction of EAO: Tx...
Autoimmune oophoritis in thymectomized mice: T cell requirement in adoptive cell transfer.
Experimental autoimmune oophoritis characterized by rapid loss of oocytes with infiltration of lymphocytes and circulating anti-oocyte antibodies could be induced in (C57Bl/6Cr x A/JCr)F1 mice after...
Autoimmune oophoritis in thymectomized mice: detection of circulating antibodies against oocytes.
Taguchi, O, Nishizuka, Y, Sakakura, T, Kojima, A
Previous studies from our laboratory described a particular type of ovarian dysgenesis which develops in mice after neonatal thymectomy (Tx) at the critical age of 2-4 days after birth. The present...
Ichikawa, T, Taguchi, O, Takahashi, T, Ikeda, H, Takeuchi, M, Tanaka, T, ...
This paper describes the spontaneous occurrence of an autoimmune uveoretinitis in nude (nu/nu) mice reconstituted when 4 weeks old by the grafting of rat embryonic thymus. The uveoretinitis was...
Experimental autoimmune prostatitis after neonatal thymectomy in the mouse.
Taguchi, O, Kojima, A, Nishizuka, Y
Experimental autoimmune prostatitis (EAP) could be induced in (C3H/HeMs X 129/J)F1 mice by thymectomy (Tx) at 3 days (Tx-3) but not at 0 or 7 days after birth. Appearance of EAP was noticed in the...
Taxonomy and function of C1 protein kinase C homology domains.
Hurley, J. H., Newton, A. C., Parker, P. J., Blumberg, P. M., Nishizuka, Y.
C1 domains are compact alpha/beta structural units of about 50 amino acids which tightly bind two zinc ions. These domains were first discovered as the loci of phorbol ester and diacylglycerol...