Y. Ohshima

Publication List Details

Period

1988 - 2004

Number

71

Co-Authors

Electron microscopy of the strain on the Si(111)$7\times7$ surface induced by the STM tip (2001)

Naitoh, Y., Takayanagi, K., Ohshima, Y., Hirayama, H.

The Si(111)7x7 surface was observed by reflection electron microscopy (REM) and scanning tunneling microscopy (STM) simultaneously in an ultra-high vacuum electron microscope. The distance between...

HYDROGEN-BONDING NETWORK AND NONRADIATIVE DYNAMICS OF HYDRATED ACRIDINE CLUSTERS (2001)

Mitsui, M., Ohshima, Y., Kajimoto, O., Ishiuchi, S., Sakai, M., Fujii, M.

$^{a}$M. Mitsui, Y. Ohshima, S. Ishiuchi, M. Sakai, and M. Fujii, Chem. Phys. Lett. 317, 211 (2000). $^{b}$B. C. Lim, J. Chem. Phys. 90, 6770 (1986).

$S_{1}-S_{0}$ VIBRONIC SPECTRA OF BENZENE CLUSTERS REVISITED: I. THE TETRAMER (2001)

Iimori, T., Ohshima, Y.

$^{a}$T. Iimori and Y. Ohshima, J. Chem. Phys. 114 2867 (2001). $^{b}$J. B. Hopkins, D. E. Powers and R. E. Smalley, J. Phys. Chem. 84 3739 (1981). $^{c}$O. Engkvist, P. Hobza, H. L. Selzle and E. W....

$S_{1}-S_{0}$ VIBRONIC SPECTRA OF BENZENE CLUSTERS REVISITED: II. THE TRIMER (2001)

Iimori, T., Aoki, Y., Ohshima, Y.

$^{a}$T. Iimori and Y. Ohshima, J. Chem. Phys. 114, 2867 (2001). $^{b}$W. Scherzer, O. Kratzschmar, H. L. Selzle, and E. W. Schlag, Z. Naturforsch. 47a, 1248 (1992). $^{c}$O. Engkvist, P. Hobza, H....

DETECTION OF TWO ISOMERS OF THE CYANOVINYL RADICAL: $H_{2}CCCN$ AND CHCHCN BY FTMW SPECTROSCOPY (2000)

Tang, J., Seiki, K., Sumiyoshi, Y., Endo, Y., Ohshima, Y.

Author Institution: Department of Basic Science, The University of Tokyo; Department of Chemistry, Kyoto University

ROTATIONAL SPECTRUM OF $N_{2}-CO$: THE $ORTHO-N_{2}$ AND $PARA-N_{2}$ (1999)

Kawashima, Y., Ohshima, Y., Endo, Y.

$^{a}$ Y. Kawashima and K. Nishizawa, Chem. Phys. Lett. 249, 87 (1996) $^{b}$ Y. Xu and A.R.W. McKellar, J. Chem. Phys. 104, 2488 (1996).

PURE ROTATIONAL SPECTRA OF RARE GAS-$HCO^{+}$ COMPLEXES (1997)

SEKI, K., SUMIYOSHI, Y., ENDO, Y., OHSHIMA, Y.

$^{m}$Y. Ohshima, Y. Sumiyoshi, and Y. Endo, 51st International Symposium on Molecular Spectroscopy, Paper WF05 (1996). $^{n}$A. Nowek and J. Leszczynski, {J. Chem. Phys}., \textbf{105}, 6388 (1996).

LASER INDUCED FLUORESCENCE SPECTROSCOPY OF THE $C_{4}H$ AND $C_{4}D$ RADICALS (1996)

HOSHINA, K., KOHGUCHI, H., ENDO, Y., OHSHIMA, Y.

$^{a}$C. A. Gottlieb, E. W. Gottlieb, P. Thaddeus, and M. Kawamura, Astrophys. J. 275, 916 (1983). $^{b}$D. R. Woodward, J. C. Person, C. A. Gottlieb,and C. A. Thaddeus, Astrophys. J. 333, L29...

Measurement of inclusive particle spectra and test of MLLA prediction in e+e- annihilation at \Sqrt s = 58 GeV (1995)

Itoh, R, Yamauchi, M, Yamaguchi, A, Abe, K, Abe, T, Adachi, I, ...

Inclusive momentum spectra are measured for all charged particles and for each of \pi^{\pm}, K^{\pm}, K^0/\overline{K^0}, and p/\overline{p} in hadronic events produced via e^+e^- annihilation at...

PDN-FTMW SPECTROSCOPY OF THE Kr-OH COMPLEX (1995)

Sato, K., Ohshima, Y., Endo, Yasuki

$^{1}$. Y. Ohshima, M. Iida, and Y. Endo, J. Chem. Phys. 95, 7001 (1991). $^{2}$. Y. Endo, H. Kohguchi, and Y. Ohshima, Faraday Discuss. 97, 341 (1994).

FTMW SPECTRA OF $NaBH_{4}$ and $KBH_{4}$ (1993)

Kawashima, Y., Ohshima, Y., Endo, Y., Hirota, E.

Author Institution: Kanagawa Institute of Technology; Department of Pure and Applied Science, The College of Arts and Sciences, The University of Tokyo; Department of Pure and Applied Science, The...

PULSED LASER-MICROWAVE DOUBLE RESONANCE STUDY OF $SO_{2}CT-XT$ BAND (1990)

Endo, Y., Ohshima, Y.

Author Institution: Department of Pure and Applied Sciences, College of Arts and Sciences, The University of Tokyo

Cloning and characterization of a human DEAH-box RNA helicase, a functional homolog of fission yeast Cdc28/Prp8.

Imamura, O, Saiki, K, Tani, T, Ohshima, Y, Sugawara, M, Furuichi, Y

During the splicing process, spliceosomal snRNAs undergo numerous conformational rearrangements that appear to be catalyzed by proteins belonging to the DEAD/H-box superfamily of RNA helicases. We...

The fission yeast prp10(+) gene involved in pre-mRNA splicing encodes a homologue of highly conserved splicing factor, SAP155.

Habara, Y, Urushiyama, S, Tani, T, Ohshima, Y

In the fission yeast Schizosaccharomyces pombe, 14 prp (pre-mRNAprocessing) mutants have been isolated to date. We cloned the prp10(+) gene by complementation of the temperature-sensitive growth of...

Mechanistic study of inhibition of levofloxacin absorption by aluminum hydroxide.

Tanaka, M, Kurata, T, Fujisawa, C, Ohshima, Y, Aoki, H, Okazaki, O, ...

The mechanisms of reduction in absorption of levofloxacin (LVFX) by coadministration of aluminum hydroxide were studied. The partition coefficient of LVFX (0.1 mM) between chloroform and phosphate...

Antiphagocytic effect of the capsule of Staphylococcus simulans.

Ohshima, Y, Schumacher-Perdreau, F, Peters, G, Quie, P G, Pulverer, G

An encapsulated strain of Staphylococcus simulans was observed to be more resistant to phagocytosis by human granulocytes than was a nonencapsulated strain. Phagocytosis of the encapsulated strain...

Cell surface antigen of encapsulated Staphylococcus epidermidis ATCC 31432.

Ohshima, Y, Ichiman, Y, Usui, Y, Ko, H L, Beuth, J, Pulverer, G, ...

The cell surface antigen (CSA) from encapsulated Staphylococcus epidermidis ATCC 31432 was isolated and fractionated by DEAE-Sephadex ion-exchange chromatography. It yielded a single precipitin line...

A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase.

Lundgren, K, Allan, S, Urushiyama, S, Tani, T, Ohshima, Y, Frendewey, D, ...

The fission-yeast gene cdc28+ was originally identified in a screen for temperature-sensitive mutants that exhibit a cell-division cycle arrest and was found to be required for mitosis. We undertook...

Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe.

Azad, A K, Tani, T, Shiki, N, Tsuneyoshi, S, Urushiyama, S, Ohshima, Y

Nucleocytoplasmic transport of mRNA is essential for eukaryotic gene expression. However, how mRNA is exported from the nucleus is mostly unknown. To elucidate the mechanisms of mRNA transport, we...

Novel models for RNA splicing that involve a small nuclear RNA.

Ohshima, Y, Itoh, M, Okada, N, Miyata, T

Nucleotide sequences of mammalian small nuclear RNAs (snRNAs) have been analyzed with a computer program for complementarity with sequences around a splice junction of various eukaryotic mRNA...

A pseudogene cluster in the leader region of the Euglena chloroplast 16S-23S rRNA genes.

Miyata, T, Kikuno, R, Ohshima, Y

The nucleotide sequence of a region (leader region) preceding the 5'-end of 16S-23S rRNA gene region of Euglena gracilis chloroplast DNA was compared with the homologous sequences that code for the...

Structural analysis of gene loci for rat U1 small nuclear RNA.

Watanabe-Nagasu, N, Itoh, Y, Tani, T, Okano, K, Koga, N, Okada, N, ...

Four phage clones which hybridize with U1 small nuclear RNA were obtained from a rat gene library. Two clones contain a presumed pseudogene. A third clone includes two gene candidates that are...

Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA.

Ohshima, Y, Okada, N, Tani, T, Itoh, Y, Itoh, M

We have isolated four clones which hybridize with U6 (4.8S) nuclear RNA, a mammalian small nuclear RNA(nRNA), from DNA of BALB/C mouse liver. Their restriction maps are totally different from each...

Beta-globin transcripts carrying a single intron with three adjacent nucleotides of 5' exon are efficiently spliced in vitro irrespective of intron position or surrounding exon sequences.

Mayeda, A, Ohshima, Y

To examine the role of exon sequences and intron position in the splicing of an mRNA precursor, we prepared series of sense or anti-sense transcripts of human beta-globin cDNA in which a cassette...

Synthesis of N2, N2, 7-trimethylguanosine cap derivatives.

Iwase, R, Sekine, M, Tokumoto, Y, Ohshima, Y, Hata, T

Several derivatives of N2,N2-7-trimethylguanosine (m3(2,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine...

Three distinct activities possibly involved in mRNA splicing are found in a nuclear fraction lacking U1 and U2 RNA.

Mayeda, A, Tatei, K, Kitayama, H, Takemura, K, Ohshima, Y

A nuclear extract from HeLa cells was fractionated by DEAE-Sepharose chromatography, and the fractions were assayed for the binding activity for a small RNA transcript carrying a splice junction or...

Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site.

Aroian, R V, Levy, A D, Koga, M, Ohshima, Y, Kramer, J M, Sternberg, P W

The dinucleotide AG, found at the 3' end of virtually all eukaryotic pre-mRNA introns, is thought to be essential for splicing. Reduction-of-function mutations in two Caenorhabditis elegans genes,...

An mRNA-type intron is present in the Rhodotorula hasegawae U2 small nuclear RNA gene.

Takahashi, Y, Urushiyama, S, Tani, T, Ohshima, Y

Splicing an mRNA precursor requires multiple factors involving five small nuclear RNA (snRNA) species called U1, U2, U4, U5, and U6. The presence of mRNA-type introns in the U6 snRNA genes of some...

Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.

Mayeda, A, Ohshima, Y

We constructed SP6-human beta-globin derivative plasmids that included possible donor site (5' splice site) sequences at a specified position within the first intron. The runoff transcripts from...

U1 RNA-protein complex preferentially binds to both 5' and 3' splice junction sequences in RNA or single-stranded DNA.

Tatei, K, Takemura, K, Mayeda, A, Fujiwara, Y, Tanaka, H, Ishihama, A, ...

We have investigated factors that recognize the splice junctions for mRNA by means of a rapid and sensitive filter binding assay using chemically synthesized single-stranded (ss) DNA (16-21...

Assumed initiation site of fibroin gene transcription.

Tsuda, M, Ohshima, Y, Suzuki, Y

To map the initiation site of fibroin gene transcription on cloned genomic fibroin DNA (pFb29), we looked for precursors of fibroin mRNA, first by applying the hybridization mapping method to the RNA...

Cloning of the silk fibroin gene and its flanking sequences.

Ohshima, Y, Suzuki, Y

Thirteen Escherichia coli clones containing the whole or a part of the fibroin gene (16 kilobases long) have been isolated. The starting material was DNA extracted from the posterior silk glands of...

Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.

Habara, Y, Urushiyama, S, Shibuya, T, Ohshima, Y, Tani, T

prp12-1 is one of the mutants defective in pre-mRNA splicing at a nonpermissive temperature in Schizosaccharomyces pombe. We found that the prp12+ gene encodes a protein highly homologous with a...

Activity of chimeric RNAs of U6 snRNA and (-)sTRSV in the cleavage of a substrate RNA.

Tani, T, Takahashi, Y, Ohshima, Y

U6 small nuclear RNA is one of the spliceosomal RNAs essential for pre-mRNA splicing. Discovery of mRNA-type introns in the highly conserved region of the U6 snRNA genes led to the hypothesis that U6...

The Prp1(+) Gene Required for Pre-mRNA Splicing in Schizosaccharomyces Pombe Encodes a Protein That Contains Tpr Motifs and Is Similar to Prp6p of Budding Yeast

Urushivama, S., Tani, T., Ohshima, Y.

The prp (pre-mRNA processing) mutants of the fission yeast Schizosaccharomyces pombe have a defect in pre-mRNA splicing and accumulate mRNA precursors at a restrictive temperature. One of the prp...

Characterization of the ptr6(+) gene in fission yeast: a possible involvement of a transcriptional coactivator TAF in nucleocytoplasmic transport of mRNA.

Shibuya, T, Tsuneyoshi, S, Azad, A K, Urushiyama, S, Ohshima, Y, Tani, T

Transport of mRNA from the nucleus to the cytoplasm is one of the important steps in gene expression in eukaryotic cells. To elucidate a mechanism of mRNA export, we identified a novel ptr [poly(A)+...

Cloning and characterization of a human DEAH-box RNA helicase, a functional homolog of fission yeast Cdc28/Prp8.

Imamura, O, Saiki, K, Tani, T, Ohshima, Y, Sugawara, M, Furuichi, Y

During the splicing process, spliceosomal snRNAs undergo numerous conformational rearrangements that appear to be catalyzed by proteins belonging to the DEAD/H-box superfamily of RNA helicases. We...

The fission yeast prp10(+) gene involved in pre-mRNA splicing encodes a homologue of highly conserved splicing factor, SAP155.

Habara, Y, Urushiyama, S, Tani, T, Ohshima, Y

In the fission yeast Schizosaccharomyces pombe, 14 prp (pre-mRNAprocessing) mutants have been isolated to date. We cloned the prp10(+) gene by complementation of the temperature-sensitive growth of...

Mechanistic study of inhibition of levofloxacin absorption by aluminum hydroxide.

Tanaka, M, Kurata, T, Fujisawa, C, Ohshima, Y, Aoki, H, Okazaki, O, ...

The mechanisms of reduction in absorption of levofloxacin (LVFX) by coadministration of aluminum hydroxide were studied. The partition coefficient of LVFX (0.1 mM) between chloroform and phosphate...

Antiphagocytic effect of the capsule of Staphylococcus simulans.

Ohshima, Y, Schumacher-Perdreau, F, Peters, G, Quie, P G, Pulverer, G

An encapsulated strain of Staphylococcus simulans was observed to be more resistant to phagocytosis by human granulocytes than was a nonencapsulated strain. Phagocytosis of the encapsulated strain...

Cell surface antigen of encapsulated Staphylococcus epidermidis ATCC 31432.

Ohshima, Y, Ichiman, Y, Usui, Y, Ko, H L, Beuth, J, Pulverer, G, ...

The cell surface antigen (CSA) from encapsulated Staphylococcus epidermidis ATCC 31432 was isolated and fractionated by DEAE-Sephadex ion-exchange chromatography. It yielded a single precipitin line...

A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase.

Lundgren, K, Allan, S, Urushiyama, S, Tani, T, Ohshima, Y, Frendewey, D, ...

The fission-yeast gene cdc28+ was originally identified in a screen for temperature-sensitive mutants that exhibit a cell-division cycle arrest and was found to be required for mitosis. We undertook...

Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe.

Azad, A K, Tani, T, Shiki, N, Tsuneyoshi, S, Urushiyama, S, Ohshima, Y

Nucleocytoplasmic transport of mRNA is essential for eukaryotic gene expression. However, how mRNA is exported from the nucleus is mostly unknown. To elucidate the mechanisms of mRNA transport, we...

Activity of chimeric RNAs of U6 snRNA and (-)sTRSV in the cleavage of a substrate RNA.

Tani, T, Takahashi, Y, Ohshima, Y

U6 small nuclear RNA is one of the spliceosomal RNAs essential for pre-mRNA splicing. Discovery of mRNA-type introns in the highly conserved region of the U6 snRNA genes led to the hypothesis that U6...

Novel models for RNA splicing that involve a small nuclear RNA.

Ohshima, Y, Itoh, M, Okada, N, Miyata, T

Nucleotide sequences of mammalian small nuclear RNAs (snRNAs) have been analyzed with a computer program for complementarity with sequences around a splice junction of various eukaryotic mRNA...

A pseudogene cluster in the leader region of the Euglena chloroplast 16S-23S rRNA genes.

Miyata, T, Kikuno, R, Ohshima, Y

The nucleotide sequence of a region (leader region) preceding the 5'-end of 16S-23S rRNA gene region of Euglena gracilis chloroplast DNA was compared with the homologous sequences that code for the...

Structural analysis of gene loci for rat U1 small nuclear RNA.

Watanabe-Nagasu, N, Itoh, Y, Tani, T, Okano, K, Koga, N, Okada, N, ...

Four phage clones which hybridize with U1 small nuclear RNA were obtained from a rat gene library. Two clones contain a presumed pseudogene. A third clone includes two gene candidates that are...

Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA.

Ohshima, Y, Okada, N, Tani, T, Itoh, Y, Itoh, M

We have isolated four clones which hybridize with U6 (4.8S) nuclear RNA, a mammalian small nuclear RNA(nRNA), from DNA of BALB/C mouse liver. Their restriction maps are totally different from each...

Beta-globin transcripts carrying a single intron with three adjacent nucleotides of 5' exon are efficiently spliced in vitro irrespective of intron position or surrounding exon sequences.

Mayeda, A, Ohshima, Y

To examine the role of exon sequences and intron position in the splicing of an mRNA precursor, we prepared series of sense or anti-sense transcripts of human beta-globin cDNA in which a cassette...

Synthesis of N2, N2, 7-trimethylguanosine cap derivatives.

Iwase, R, Sekine, M, Tokumoto, Y, Ohshima, Y, Hata, T

Several derivatives of N2,N2-7-trimethylguanosine (m3(2,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine...

Three distinct activities possibly involved in mRNA splicing are found in a nuclear fraction lacking U1 and U2 RNA.

Mayeda, A, Tatei, K, Kitayama, H, Takemura, K, Ohshima, Y

A nuclear extract from HeLa cells was fractionated by DEAE-Sepharose chromatography, and the fractions were assayed for the binding activity for a small RNA transcript carrying a splice junction or...

Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site.

Aroian, R V, Levy, A D, Koga, M, Ohshima, Y, Kramer, J M, Sternberg, P W

The dinucleotide AG, found at the 3' end of virtually all eukaryotic pre-mRNA introns, is thought to be essential for splicing. Reduction-of-function mutations in two Caenorhabditis elegans genes,...

An mRNA-type intron is present in the Rhodotorula hasegawae U2 small nuclear RNA gene.

Takahashi, Y, Urushiyama, S, Tani, T, Ohshima, Y

Splicing an mRNA precursor requires multiple factors involving five small nuclear RNA (snRNA) species called U1, U2, U4, U5, and U6. The presence of mRNA-type introns in the U6 snRNA genes of some...

Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.

Mayeda, A, Ohshima, Y

We constructed SP6-human beta-globin derivative plasmids that included possible donor site (5' splice site) sequences at a specified position within the first intron. The runoff transcripts from...

U1 RNA-protein complex preferentially binds to both 5' and 3' splice junction sequences in RNA or single-stranded DNA.

Tatei, K, Takemura, K, Mayeda, A, Fujiwara, Y, Tanaka, H, Ishihama, A, ...

We have investigated factors that recognize the splice junctions for mRNA by means of a rapid and sensitive filter binding assay using chemically synthesized single-stranded (ss) DNA (16-21...

Assumed initiation site of fibroin gene transcription.

Tsuda, M, Ohshima, Y, Suzuki, Y

To map the initiation site of fibroin gene transcription on cloned genomic fibroin DNA (pFb29), we looked for precursors of fibroin mRNA, first by applying the hybridization mapping method to the RNA...

Cloning of the silk fibroin gene and its flanking sequences.

Ohshima, Y, Suzuki, Y

Thirteen Escherichia coli clones containing the whole or a part of the fibroin gene (16 kilobases long) have been isolated. The starting material was DNA extracted from the posterior silk glands of...

The Prp1(+) Gene Required for Pre-mRNA Splicing in Schizosaccharomyces Pombe Encodes a Protein That Contains Tpr Motifs and Is Similar to Prp6p of Budding Yeast

Urushivama, S., Tani, T., Ohshima, Y.

The prp (pre-mRNA processing) mutants of the fission yeast Schizosaccharomyces pombe have a defect in pre-mRNA splicing and accumulate mRNA precursors at a restrictive temperature. One of the prp...

Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.

Habara, Y, Urushiyama, S, Shibuya, T, Ohshima, Y, Tani, T

prp12-1 is one of the mutants defective in pre-mRNA splicing at a nonpermissive temperature in Schizosaccharomyces pombe. We found that the prp12+ gene encodes a protein highly homologous with a...

Characterization of the ptr6(+) gene in fission yeast: a possible involvement of a transcriptional coactivator TAF in nucleocytoplasmic transport of mRNA.

Shibuya, T, Tsuneyoshi, S, Azad, A K, Urushiyama, S, Ohshima, Y, Tani, T

Transport of mRNA from the nucleus to the cytoplasm is one of the important steps in gene expression in eukaryotic cells. To elucidate a mechanism of mRNA export, we identified a novel ptr [poly(A)+...