Scientific Commons: Y. Pommier

This article not subject to U.S. Copyright. Published 1998 by the American Chemical Society

Interaction of an alkylating camptothecin derivative with a DNA base at topoisomerase I-DNA cleavage sites.

Pommier, Y, Kohlhagen, G, Kohn, K W, Leteurtre, F, Wani, M C, Wall, M E

DNA topoisomerase I (top1) is a ubiquitous nuclear enzyme. It is specifically inhibited by camptothecin, a natural product derived from the bark of the tree Camptotheca acuminata. Camptothecin and...

Inhibition of human immunodeficiency virus type 1 integrase by 3'-azido-3'-deoxythymidylate.

Mazumder, A, Cooney, D, Agbaria, R, Gupta, M, Pommier, Y

The effects of 3'-azido-3'-deoxythymidine (AZT) and three of its intracellular metabolites, azido- thymidine mono-, di-, and triphosphates, on the human immunodeficiency virus type 1 integrase have...

Inhibitors of human immunodeficiency virus integrase.

Fesen, M R, Kohn, K W, Leteurtre, F, Pommier, Y

In an effort to further extend the number of targets for development of antiretroviral agents, we have used an in vitro integrase assay to investigate a variety of chemicals, including topoisomerase...

In vivo sequencing of camptothecin-induced topoisomerase I cleavage sites in human colon carcinoma cells.

Pondarré, C, Strumberg, D, Fujimori, A, Torres-León, R, Pommier, Y

Camptothecin (CPT) is a specific topoisomerase I (top1) poison which traps top1 cleavable complexes; e.g. top1-linked DNA single-strand breaks with 5'-hydroxyl and 3'-top1 linked termini. CPT is also...

Human DNA topoisomerase I-mediated cleavage and recombination of duck hepatitis B virus DNA in vitro.

Pourquier, P, Jensen, A D, Gong, S S, Pommier, Y, Rogler, C E

In this study, we report that eukaryotic topoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B virus (DHBV). Using synthetic oligonucleotides mimicking the three-strand flap...

T30177, an oligonucleotide stabilized by an intramolecular guanosine octet, is a potent inhibitor of laboratory strains and clinical isolates of human immunodeficiency virus type 1.

Ojwang, J O, Buckheit, R W, Pommier, Y, Mazumder, A, De Vreese, K, Esté, J A, ...

T30177, an oligonucleotide composed of only deoxyguanosine and thymidine, is 17 nucleotides in length and contains single phosphorothioate internucleoside linkages at its 5' and 3' ends for...

Diarylsulfones, a novel class of human immunodeficiency virus type 1 integrase inhibitors.

Neamati, N, Mazumder, A, Zhao, H, Sunder, S, Burke, T R, Schultz, R J, ...

A majority of reported human immunodeficiency virus type 1 integrase (HIV-1 IN) inhibitors are polyhydroxylated aromatic compounds containing two phenyl rings separated by aliphatic or aromatic...

Identification within the simian virus 40 genome of a chromosomal loop attachment site that contains topoisomerase II cleavage sites.

Pommier, Y, Cockerill, P N, Kohn, K W, Garrard, W T

We demonstrate that the simian virus 40 genome contains a single MAR (matrix association region) that maps within a large T-antigen coding region (nucleotides 4071 to 4377). This region contains...

DNA unwinding and inhibition of mouse leukemia L1210 DNA topoisomerase I by intercalators.

Pommier, Y, Covey, J M, Kerrigan, D, Markovits, J, Pham, R

The DNA unwinding effects of some 9-aminoacridine derivatives were compared under reaction conditions that could be used to study drug-induced topoisomerase II inhibition. An assay was designed to...

Processing of deoxyuridine mismatches and abasic sites by human immunodeficiency virus type-1 integrase.

Mazumder, A, Pommier, Y

We have examined the activities of HIV-1 integrase on substrates containing mismatches, composed of deoxyuridine at different positions in either the processed or nonprocessed strand of viral DNA,...

Intermolecular disintegration and intramolecular strand transfer activities of wild-type and mutant HIV-1 integrase.

Mazumder, A, Engelman, A, Craigie, R, Fesen, M, Pommier, Y

We report the activities of HIV integrase protein on a novel DNA substrate, consisting of a pair of gapped duplex molecules. Integrase catalyzed an intermolecular disintegration reaction that...

Methylphosphonodiester substitution near the conserved CA dinucleotide in the HIV LTR alters both extent of 3'-processing and choice of nucleophile by HIV-1 integrase.

Mazumder, A, Gupta, M, Pommier, Y

We present evidence suggesting that the 3'-processing activity of HIV-1 integrase is dramatically affected by electrostatic and/or steric perturbations 3' to the conserved CA dinucleotide. When the...

Induction of cleavage in topoisomerase I c-DNA by topoisomerase I enzymes from calf thymus and wheat germ in the presence and absence of camptothecin.

Tanizawa, A, Kohn, K W, Pommier, Y

In this study, we further examined the sequence selectivity of camptothecin in mammalian topoisomerase I cDNA from human and Chinese hamster. In the absence of camptothecin, almost all the bases at...

Local base sequence preferences for DNA cleavage by mammalian topoisomerase II in the presence of amsacrine or teniposide.

Pommier, Y, Capranico, G, Orr, A, Kohn, K W

Several classes of antitumor drugs are known to stabilize topoisomerase complexes in which the enzyme is covalently bound to a terminus of a DNA strand break. The DNA cleavage sites generally are...

Nucleosome positioning as a critical determinant for the DNA cleavage sites of mammalian DNA topoisomerase II in reconstituted simian virus 40 chromatin.

Capranico, G, Jaxel, C, Roberge, M, Kohn, K W, Pommier, Y

We have assessed the ability of nucleosomes to influence the formation of mammalian topoisomerase II-DNA complexes by mapping the sites of cleavage induced by four unrelated topoisomerase II...

Local sequence requirements for DNA cleavage by mammalian topoisomerase II in the presence of doxorubicin.

Capranico, G, Kohn, K W, Pommier, Y

Doxorubicin, a DNA-intercalator, is one of several anti-cancer drugs that have been found to stabilizes topoisomerase II cleavage complexes at drug-specific DNA sites. The distribution and DNA...

Topoisomerase I interaction with SV40 DNA in the presence and absence of camptothecin.

Jaxel, C, Kohn, K W, Pommier, Y

Camptothecin is an antitumor drug, which is a specific inhibitor of eukaryotic topoisomerase I. Enzyme inhibition is related to the stabilization of cleavable complexes between topoisomerase I and...

111 Indium labelling of red cells: its clinical use in haemolytic anaemias.

Najean, Y, Pommier, Y, Deschrywer, F, Arrago, J P

Role of oxygen radicals generated by NADPH oxidase in apoptosis induced in human leukemia cells.

Hiraoka, W, Vazquez, N, Nieves-Neira, W, Chanock, S J, Pommier, Y

We have used a human leukemia cell line that, after homologous recombination knockout of the gp91-phox subunit of the phagocyte respiratory-burst oxidase cytochrome b-558, mimics chronic...

Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes.

Shao, R G, Cao, C X, Zhang, H, Kohn, K W, Wold, M S, Pommier, Y

Replication protein A (RPA) is a DNA single-strand binding protein essential for DNA replication, recombination and repair. In human cells treated with the topoisomerase inhibitors camptothecin or...