Bailey, K., Keyte, J. W., Chan, W. C., Greenwood, D., Mahida, Y. R.
In the human gastrointestinal tract, microorganisms are present in large numbers in the colon but are sparse in the proximal small intestine. In this study, we have shown that acid extracts of fresh...
Mahida, Y. R., Galvin, A., Makh, S., Hyde, S., Sanfilippo, L., Borriello, S. P., ...
We have previously shown that Clostridium difficile toxin A induces detachment of human colonic epithelial cells from the basement membrane and subsequent cell death by apoptosis. Because these cells...
Functional interleukin-2 receptors on intestinal epithelial cells.
Ciacci, C, Mahida, Y R, Dignass, A, Koizumi, M, Podolsky, D K
The presence of receptors for the cytokine IL-2 was assessed in the IEC-6 cell line established from normal rat crypt epithelium and primary intestinal epithelial cells. 125I-IL-2 was found to...
Coutinho, H B, Da Mota, H C, Coutinho, V B, Robalinho, T I, Furtado, A F, Walker, E, ...
AIM: To determine immunocytochemically whether preterm and newborn infants with necrotising enterocolitis (NEC) show differences in numbers of lysozyme positive Paneth cells compared with normal...
Solomon, K., Webb, J., Ali, N., Robins, R. A., Mahida, Y. R.
In this study we investigated the in vitro responses of peripheral blood mononuclear preparations and purified monocytes to Clostridium difficile toxin A. In contrast to the responses of T and B...
Bailey, K., Keyte, J. W., Chan, W. C., Greenwood, D., Mahida, Y. R.
In the human gastrointestinal tract, microorganisms are present in large numbers in the colon but are sparse in the proximal small intestine. In this study, we have shown that acid extracts of fresh...
Mahida, Y. R., Galvin, A., Makh, S., Hyde, S., Sanfilippo, L., Borriello, S. P., ...
We have previously shown that Clostridium difficile toxin A induces detachment of human colonic epithelial cells from the basement membrane and subsequent cell death by apoptosis. Because these cells...
Functional interleukin-2 receptors on intestinal epithelial cells.
Ciacci, C, Mahida, Y R, Dignass, A, Koizumi, M, Podolsky, D K
The presence of receptors for the cytokine IL-2 was assessed in the IEC-6 cell line established from normal rat crypt epithelium and primary intestinal epithelial cells. 125I-IL-2 was found to...
Coutinho, H B, Da Mota, H C, Coutinho, V B, Robalinho, T I, Furtado, A F, Walker, E, ...
AIM: To determine immunocytochemically whether preterm and newborn infants with necrotising enterocolitis (NEC) show differences in numbers of lysozyme positive Paneth cells compared with normal...
Solomon, K., Webb, J., Ali, N., Robins, R. A., Mahida, Y. R.
In this study we investigated the in vitro responses of peripheral blood mononuclear preparations and purified monocytes to Clostridium difficile toxin A. In contrast to the responses of T and B...
Early cytokine responses during intestinal parasitic infections.
Ishikawa, N, Goyal, P K, Mahida, Y R, Li, K F, Wakelin, D
Infections with gastro-intestinal nematodes elicit immune and inflammatory responses mediated by cytokines released from T-helper type-2 (Th2) cells. In vitro assays of cells from the mesenteric...
Mahida, Y R, Wu, K C, Jewell, D P
Antigen-presenting activity in mononuclear cells, isolated from normal and inflamed human ileum and colon, has been characterized using allogeneic mixed lymphocyte reaction with resting T cells as...
Senju, M, Wu, K C, Mahida, Y R, Jewell, D P
Using two colour immunofluorescence with fluorescein isothiocyanate and phycoerythrin labelled monoclonal antibodies and multiparameter flow cytometry, we investigated the coexpression of CD4 and CD8...
Mahida, Y R, Lamming, C E, Gallagher, A, Hawthorne, A B, Hawkey, C J
Interleukin 1 beta in biopsy specimens from inflamed colonic mucosa of patients with active inflammatory bowel disease was studied. Compared with normal colonic mucosal biopsy specimens, a...
Mahida, Y R, Kurlac, L, Gallagher, A, Hawkey, C J
Peripheral venous plasma concentrations of interleukin-6 were studied in 21 patients with active Crohn's disease, 20 patients with ulcerative colitis, and 16 control subjects. Interleukin-6 was...
Mahida, Y R, Makh, S, Hyde, S, Gray, T, Borriello, S P
Clostridium difficile is the aetiological agent of pseudomembranous colitis, and animal studies suggest the essential role of secreted toxin A in inducing disease. This study examined the biological...
Comparative modes of action of lactitol and lactulose in the treatment of hepatic encephalopathy.
Patil, D H, Westaby, D, Mahida, Y R, Palmer, K R, Rees, R, Clark, M L, ...
Lactitol, an unabsorbed sugar with defined laxative threshold and superior taste properties has been suggested as an alternative to lactulose in the treatment of hepatic encephalopathy. In the...
Mahida, Y R, Wu, K, Jewell, D P
IL1-beta production by mononuclear cells isolated from normal and active inflammatory bowel disease mucosa was studied. Significantly more IL1-beta was produced spontaneously by mononuclear cells...
Macrophage subpopulations in lamina propria of normal and inflamed colon and terminal ileum.
Mahida, Y R, Patel, S, Gionchetti, P, Vaux, D, Jewell, D P
The aim of this study was to characterise human intestinal macrophages in normal and inflamed ileum and colon. Immunoperoxidase staining with a panel of monoclonal antibodies and histochemical...
Respiratory burst activity of intestinal macrophages in normal and inflammatory bowel disease.
Mahida, Y R, Wu, K C, Jewell, D P
Macrophages isolated from normal mucosa (greater than 5 cm from tumour) and inflamed mucosa (from patients with inflammatory bowel disease) of colon and ileum were studied for their ability to...
Johal, S S, Hammond, J, Solomon, K, James, P D, Mahida, Y R
Objectives: Clostridium difficile associated diarrhoea (CDAD) is a hospital acquired infection in which optimal methods for diagnosis and the scale of the problem in the community remain to be...
Ranitidine, aspirin, food, and the stomach.
Cole, A. T., Brundell, S., Hudson, N., Hawthorne, A. B., Mahida, Y. R., Hawkey, C. J.
Cunliffe, R N, Kamal, M, James, P D, Mahida, Y R
Background/Aims: The normal intestinal epithelium is increasingly being recognised as an important component of the mucosal innate protection against microorganisms. Human neutrophil defensins 1–3...
Wu, K C, Mahida, Y R, Priddle, J D, Jewell, D P
We studied immunoglobulin production by isolated intestinal mononuclear cells from 25 patients with active inflammatory bowel disease (IBD) and 17 controls undergoing surgical resections for...
Wu, K C, Mahida, Y R, Priddle, J D, Jewell, D P
The effect of macrophages on spontaneous immunoglobulin production by isolated human intestinal mononuclear cells (MNC) is unknown. Depletion of macrophages by adherence to fibronectin or by panning...
Plasma and tissue interleukin-2 receptor levels in inflammatory bowel disease.
Mahida, Y R, Gallagher, A, Kurlak, L, Hawkey, C J
Plasma and tissue interleukin-2 receptor (IL-2R) levels were determined in patients with active ulcerative colitis and Crohn's disease. Compared with healthy controls (median 440 U/ml; range...
Macrophage and lymphocyte subpopulations in magnifying endoscopic lesions of Crohn's disease.
Gionchetti, P, Mahida, Y R, Patel, S, Jewell, D P
Using dye staining and a magnifying colonoscope, small lesions in the rectum of patients with Crohn's disease (which had otherwise appeared normal on sigmoidoscopy) can be detected. Subpopulations of...
Mahida, Y R, Patel, S, Jewell, D P
Macrophages in sections of human terminal ileal Peyer's patches were studied using a panel of monoclonal antibodies. Germinal centre macrophages were large and strongly positive for acid phosphatase...
Interleukin 2 receptor expression by macrophages in inflammatory bowel disease.
Mahida, Y R, Patel, S, Wu, K, Jewell, D P
The expression of interleukin 2 receptor by macrophages from normal and inflamed terminal ileum and colon has been studied by using two monoclonal antibodies. In tissue sections from normal ileum and...
MAHIDA, Y R, GALVIN, A M, GRAY, T, MAKH, S, McALINDON, M E, SEWELL, H F, ...
Lymphocytes and macrophages are present in the normal intestinal lamina propria, separated from the epithelial monolayer by the basement membrane. There is evidence for movement of mononuclear cells...
McAlindon, M E, Galvin, A, McKaig, B, Gray, T, Sewell, H F, Mahida, Y R
Activated mucosal macrophages are derived from circulating monocytes and appear to play a major role in the pathogenesis of IBD. We have recently shown that IBD, but not normal, mucosal macrophages...
Sanfilippo, L, Li, C K F, Seth, R, Balwin, T J, Menozzi, M G, Mahida, Y R
Bacteroides fragilis toxin (BFT) has been shown to be capable of inducing intestinal mucosal inflammation in animals. Such inflammation may be responsible for diarrhoea, which occurs in some, but not...
Kamal, M, Wakelin, D, Ouellette, A J, Smith, A, Podolsky, D K, Mahida, Y R
Secretions of Paneth, intermediate and goblet cells have been implicated in innate intestinal host defense. We have investigated the role of T cells in effecting alterations in small intestinal...
Selective reduction of intestinal trefoil factor in untreated coeliac disease
CIACCI, C, DI VIZIO, D, SETH, R, INSABATO, G, MAZZACCA, G, PODOLSKY, D K, ...
The trefoil factor family (TFF) encompasses small peptides of which intestinal trefoil factor (ITF) is expressed specifically in goblet cells of the small and large intestine. Previous studies have...
Johal, S S, Lambert, C P, Hammond, J, James, P D, Borriello, S P, Mahida, Y R
Background: In Clostridium difficile associated diarrhoea (CDAD), histological changes in the colonic mucosa range from minimal inflammation to pseudomembranous colitis (PMC). The disease also recurs...
Hawthorne, A B, Mahida, Y R, Cole, A T, Hawkey, C J
1. Gastric damage induced by low-dose aspirin and the protective effect of enteric-coating was assessed in healthy volunteers in a double-blind placebo-controlled cross-over trial using Latin square...
Mahida, Y. R., Chapman, R. W., Jewell, D. P.
Three cases of chronic pancreatitis occurring in patients with small intestinal diverticulosis and bacterial overgrowth are reported. In two of the cases, pancreatic supplements were therapeutically...
Clostridium difficile-associated diarrhoea.
Wight, N., Curtis, H., Hyde, J., Borriello, S. P., Mahida, Y. R.
At our hospital, the number of cases of Clostridium difficile-associated diarrhoea increased from 29 in 1993 to 210 in 1995. The case notes of 110 patients with C difficile-associated diarrhoea...
Sutton, P A, Li, S, Webb, J, Solomon, K, Brazier, J, Mahida, Y R
Clostridium difficile induces mucosal inflammation via secreted toxins A and B and initial interactions between the toxins and intestinal epithelial cells (which lead to loss of barrier function) are...