SNP and haplotype mapping for genetic analysis in the rat (2008)
The STAR Consortium, Saar, K, Beck, Alfred, Bihoreau, MT, Birney, E, Brocklebank D, D, ...
The laboratory rat is one of the most extensively studied model organisms. Inbred laboratory rat strains originated from limited Rattus norvegicus founder populations, and the inherited genetic...
DNA sequence and comparative analysis of chimpanzee chromosome 22 (2004)
Watanabe, H., Fujiyama, A., Hattory, M., Taylor, T. D., Toyoda, A., Kuroki, Y., ...
DIGIT: a novel gene finding program by combining gene-finders (2003)
T. Yada, T. Takagi, Y. Totoki, Y. Sakaki, Y. Takaeda
We have developed a general purpose algorithm which finds genes by combining plural existing gene-finders. The algorithm has been implemented into a novel gene-finder named DIGIT. An outline of the...
Initial sequencing and analysis of the human genome (2001)
Lander, ES, Linton, LM, Birren, B, Nusbaum, C, Zody, MC, Baldwin, J, ...
The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and...
Uematsu, C., Nishida, J., Okano, K., Miura, F., Ito, T., Sakaki, Y., ...
A method based on the multiplex polymerase chain reaction (PCR) and gel electrophoresis for the comparative analysis of gene expression levels was developed. Using the method many cDNA fragments from...
Imai, T, Baba, M, Shoudai, K, Ishizuka, K, Nakagawa, T, ...
By searching the expressed sequence tag (EST) database, we identified partial cDNA sequences encoding a polypeptide with significant sequence identity to the human CC chemokine...
Fiten, P, Nomiyama, H, Sakaki, Y, Miura, R, Yoshie, O, ...
Monocyte chemotactic proteins (MCPs) form a subfamily of chemokines that recruit leukocytes to sites of inflammation and that may contribute to tumor-associated leukocyte infiltration and to the...
Imai, T, Kusuda, J, Tei, H, ...
Partial overlapping cDNA sequences likely to encode a novel human CC chemokine were identified from the GenBank Expressed Sequence Tag data base. Using these sequences, we isolated full-length cDNA...
Iron Loss Analysis of Ferrite Cores (1997)
Saotome, H., Kawai, T., Sakaki, Y.
Iron loss measurement of ferrite cores up to MHz range is carried out. Taking the DC magnetic hysteresis, the eddy and displacement currents into account, the electromagnetic fields and iron losses...
Iron Loss Analysis of Ferrite Cores (1997)
Saotome, H., Kawai, T., Sakaki, Y.
Iron loss measurement of ferrite cores up to MHz range is carried out. Taking the DC magnetic hysteresis, the eddy and displacement currents into account, the electromagnetic fields and iron losses...
Iron Loss Analysis of Ferrite Cores (1997)
Saotome, H., Kawai, T., Sakaki, Y.
Iron loss measurement of ferrite cores up to MHz range is carried out. Taking the DC magnetic hysteresis, the eddy and displacement currents into account, the electromagnetic fields and iron losses...
Gene identification in 1.6-Mb region of the Down syndrome region on chromosome 21 (1997)
Ohira, M, Seki, N, Nagase, T, Suzuki, E, Nomura, N, Ohara, O, ...
A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2 (1996)
Ueno, M, Satoh, T, Nomiyama, H, Tei, H, Takeda, M, ...
CC chemokines are cytokines that attract and activate leukocytes. The human genes for the CC chemokines are clustered on chromosome 17. To elucidate the genomic organization of the CC chemokine...
GENAS: a database system for nucleic acid sequence analysis (1984)
Kuhara, S, Matsuo, F, Futamura, S, Fujita, A, Shinohara, T, Takagi, T, ...
New 500 kV Electron Microscope (1965)
TADANO, B., KIMURA, H., KATAGIRI, S., NISHIGAKI, M., UYEDA, R., SAKAKI, Y., ...
A new high-voltage electron microscope is completed. A 10-stage electron gun with a Cockcroft-Walton type of accelerator of the same stages is enclosed in a pressure tank of 3 kg/cm2 Freon gas. The...
High Voltage (300KV) Electron Microscope and its Applications (1959)
TADANO, B, KATAGIRI, S., ICHIGE, K., SAKAKI, Y., MARUSE, S.
A spray device of the van de Graaff generator for 300 kV electron microscope was newly designed so that the high voltage output has the stability of 10−4/30 sec. sufficient for obtaining the...
Nakashima, K, Nobuhisa, I, Deshimaru, M, Nakai, M, Ogawa, T, Shimohigashi, Y, ...
The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes...
Accelerated evolution of Trimeresurus flavoviridis venom gland phospholipase A2 isozymes.
Nakashima, K, Ogawa, T, Oda, N, Hattori, M, Sakaki, Y, Kihara, H, ...
Six Trimeresurus flavoviridis (Habu snake) venom gland phospholipase A2 (PLA2) isozyme genes were found to consist of four exons and three introns and to encode proteins of 138 amino acid residues,...
Ogawa, T, Oda, N, Nakashima, K, Sasaki, H, Hattori, M, Sakaki, Y, ...
As a step toward understanding the structure and function of phospholipases A2 (PLA2s), we isolated and sequenced several cDNAs encoding Trimeresurus flavoviridis venom PLA2 isozymes including two...
Uematsu, C., Nishida, J., Okano, K., Miura, F., Ito, T., Sakaki, Y., ...
A method based on the multiplex polymerase chain reaction (PCR) and gel electrophoresis for the comparative analysis of gene expression levels was developed. Using the method many cDNA fragments from...
Hattori, M, Tsukahara, F, Furuhata, Y, Tanahashi, H, Hirose, M, Saito, M, ...
We developed a novel in vitro method for making nested deletions and applied it to a large-scale DNA sequencing. A DNA fragment to be sequenced (up to 15 kb long) was cloned with a new vector...
Bioluminescence of the Ca2+-binding photoprotein aequorin after cysteine modification.
Kurose, K, Inouye, S, Sakaki, Y, Tsuji, F I
Aequorin is a monomeric Ca2+-binding protein (Mr, 21,400) that emits light upon reacting with Ca2+. The protein has three Ca2+-binding sites, three cysteine residues, and a noncovalently bound...
Molecular cloning of cDNAs of human liver and placenta NADH-cytochrome b5 reductase.
Yubisui, T, Naitoh, Y, Zenno, S, Tamura, M, Takeshita, M, Sakaki, Y
A cDNA coding for human liver NADH-cytochrome b5 reductase (cytochrome b5 reductase, EC 1.6.2.2) was cloned from a human liver cDNA library constructed in phage lambda gt11. The library was screened...
A HindIII polymorphism detected by the cDNA encoding amyloid beta protein of Alzheimer's disease.
Sasaki, H, Oishi, N, Furuya, H, Yoshioka, K, Yamada, T, Ogawa, S, ...
Kurose, K, Hata, K, Hattori, M, Sakaki, Y
From the general views of the eukaryotic transcription systems, L1 (or L1-like) retrotransposons that encode some proteins are unusual. L1, unlike other protein-coding elements, is transcribed...
The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
Miyoshi, J, Kagimoto, M, Soeda, E, Sakaki, Y
The human c-Ha-ras2 gene, one of two known members of the Harvey ras family, is reportedly located on the X-chromosome and has lost introns (1, 2). There has heretofore been no information on its...
Direct repeats surrounding the ribosomal RNA genes of Physarum polycephalum.
Hattori, M, Ljljana, A, Sakaki, Y
Sequence homology was found between the external transcribed spacer and the terminal non-transcribed spacer of Physarum polycephalum rDNA. The homologous sequences were located 2kb upstream from the...
Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum.
Nomiyama, H, Sakaki, Y, Takagi, Y
The Physarum polycephalum 26S ribosomal RNA gene contains two intervening sequences (introns). The DNA sequence of one of these introns was analyzed together with that of its flanking regions...
Nucleotide sequence of Physarum polycephalum 5.8S rRNA gene and its flanking regions.
Otsuka, T, Nomiyama, H, Sakaki, Y, Takagi, Y
The nucleotide sequence of Physarum polycephalum 5.8S rRNA gene and its flanking regions has been determined. The homologies of the 5.8S rRNA sequence with those of Saccharomyces, Chlamydomonas and...
GENAS: a database system for nucleic acid sequence analysis.
Kuhara, S, Matsuo, F, Futamura, S, Fujita, A, Shinohara, T, Takagi, T, ...
A database system, named GENAS (GENe Analyzing System), for computer analysis of sequence was constructed using Adbis which is a relational database management system (1). GENAS enables us to...
Sequence analysis of a KpnI family member near the 3' end of human beta-globin gene.
Hattori, M, Hidaka, S, Sakaki, Y
We determined the complete nucleotide sequence (6125 bp) of a full-length member of human KpnI family, designated T beta G41, which is located about 3 kb downstream from the beta-globin gene....
Some KpnI family members are associated with the Alu family in the human genome.
Miyake, T, Migita, K, Sakaki, Y
The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5'...
Nomiyama, H, Kuhara, S, Kukita, T, Otsuka, T, Sakaki, Y
The 26S ribosomal RNA gene of Physarum polycephalum is interrupted by two introns, and we have previously determined the sequence of one of them (intron 1) (Nomiyama et al. Proc.Natl.Acad.Sci.USA 78,...
Hohjoh, H, Minakami, R, Sakaki, Y
L1 (LINE-1), a long interspersed repetitive DNA family of mammalian genomes, is thought to be a sequence family derived from a retrotransposon-like element(s), but its actively transposable unit(s)...
Ito, T, Tanahashi, H, Misumi, Y, Sakaki, Y
During acute inflammation, a group of liver-derived plasma proteins, acute phase proteins (APPs), increase in concentration. Interleukin-6 (IL-6) is responsible for this increase via the induction of...
A novel procedure for selective cloning of NotI linking fragments from mammalian genomes.
A novel procedure has been developed for selective cloning of NotI linking fragments from mammalian genomes. Since the majority of the NotI sites in mammalian genomes are considered to be localized...
Fujita, A, Hattori, M, Takenaka, O, Sakaki, Y
We previously reported that some L1 family (KpnI family) members are closely associated with the Alu family sequence. To understand the details of the L1-Alu association, the structure of a L1-Alu...
A bacteriophage (phiYS40) infectious to an extreme thermophile, Thermus thermophilus HB8, was isolated and characterized. phiYS40 grows over the temperature range of 56 to 78 C, and the optimum...
Otsuka, T, Nomiyama, H, Yoshida, H, Kukita, T, Kuhara, S, Sakaki, Y
The complete nucleotide sequences of the 5.8S and 26S rRNA genes of Physarum polycephalum and the transcribed spacer between them were determined. Comparison of the sequences with those of the...
Cloning and sequence analysis of cDNA for the luminescent protein aequorin.
Inouye, S, Noguchi, M, Sakaki, Y, Takagi, Y, Miyata, T, Iwanaga, S, ...
The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. A cDNA library of Aequorea was...
Structure of Met30 variant of transthyretin and its amyloidogenic implications.
Terry, C J, Damas, A M, Oliveira, P, Saraiva, M J, Alves, I L, Costa, P P, ...
Familial amyloidotic polyneuropathy (FAP) is an autosomal dominant hereditary type of lethal amyloidosis involving single (or double) amino acid substitutions in the amyloidogenic protein...
Furuya, H, Yoshioka, K, Sasaki, H, Sakaki, Y, Nakazato, M, Matsuo, H, ...
A Japanese family with atypical type I familial amyloidotic polyneuropathy (FAP) in Iiyama, Japan was studied. Most of the family members have dysfunctions in the central nervous system, in addition...
Primary structure of rat liver alkaline phosphatase deduced from its cDNA.
Misumi, Y, Tashiro, K, Hattori, M, Sakaki, Y, Ikehara, Y
Rat liver alkaline phosphatase (ALP) was markedly induced by treatment of rats by bile-duct ligation and colchicine injection. Taking this advantage for enrichment of ALP mRNA, we constructed a...
Minakami, R, Kurose, K, Etoh, K, Furuhata, Y, Hattori, M, Sakaki, Y
L1 (LINE-1) is a long interspersed repetitive sequence derived from a retrotransposon. Transfection studies using the CAT gene as a reporter demonstrated that the first 155bp in the human L1 sequence...
Nakashima, K, Nobuhisa, I, Deshimaru, M, Nakai, M, Ogawa, T, Shimohigashi, Y, ...
The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes...
Accelerated evolution of Trimeresurus flavoviridis venom gland phospholipase A2 isozymes.
Nakashima, K, Ogawa, T, Oda, N, Hattori, M, Sakaki, Y, Kihara, H, ...
Six Trimeresurus flavoviridis (Habu snake) venom gland phospholipase A2 (PLA2) isozyme genes were found to consist of four exons and three introns and to encode proteins of 138 amino acid residues,...
Ogawa, T, Oda, N, Nakashima, K, Sasaki, H, Hattori, M, Sakaki, Y, ...
As a step toward understanding the structure and function of phospholipases A2 (PLA2s), we isolated and sequenced several cDNAs encoding Trimeresurus flavoviridis venom PLA2 isozymes including two...
Uematsu, C., Nishida, J., Okano, K., Miura, F., Ito, T., Sakaki, Y., ...
A method based on the multiplex polymerase chain reaction (PCR) and gel electrophoresis for the comparative analysis of gene expression levels was developed. Using the method many cDNA fragments from...
Hattori, M, Tsukahara, F, Furuhata, Y, Tanahashi, H, Hirose, M, Saito, M, ...
We developed a novel in vitro method for making nested deletions and applied it to a large-scale DNA sequencing. A DNA fragment to be sequenced (up to 15 kb long) was cloned with a new vector...
Bioluminescence of the Ca2+-binding photoprotein aequorin after cysteine modification.
Kurose, K, Inouye, S, Sakaki, Y, Tsuji, F I
Aequorin is a monomeric Ca2+-binding protein (Mr, 21,400) that emits light upon reacting with Ca2+. The protein has three Ca2+-binding sites, three cysteine residues, and a noncovalently bound...
Molecular cloning of cDNAs of human liver and placenta NADH-cytochrome b5 reductase.
Yubisui, T, Naitoh, Y, Zenno, S, Tamura, M, Takeshita, M, Sakaki, Y
A cDNA coding for human liver NADH-cytochrome b5 reductase (cytochrome b5 reductase, EC 1.6.2.2) was cloned from a human liver cDNA library constructed in phage lambda gt11. The library was screened...
A HindIII polymorphism detected by the cDNA encoding amyloid beta protein of Alzheimer's disease.
Sasaki, H, Oishi, N, Furuya, H, Yoshioka, K, Yamada, T, Ogawa, S, ...
Kurose, K, Hata, K, Hattori, M, Sakaki, Y
From the general views of the eukaryotic transcription systems, L1 (or L1-like) retrotransposons that encode some proteins are unusual. L1, unlike other protein-coding elements, is transcribed...
Minakami, R, Kurose, K, Etoh, K, Furuhata, Y, Hattori, M, Sakaki, Y
L1 (LINE-1) is a long interspersed repetitive sequence derived from a retrotransposon. Transfection studies using the CAT gene as a reporter demonstrated that the first 155bp in the human L1 sequence...
The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
Miyoshi, J, Kagimoto, M, Soeda, E, Sakaki, Y
The human c-Ha-ras2 gene, one of two known members of the Harvey ras family, is reportedly located on the X-chromosome and has lost introns (1, 2). There has heretofore been no information on its...
Direct repeats surrounding the ribosomal RNA genes of Physarum polycephalum.
Hattori, M, Ljljana, A, Sakaki, Y
Sequence homology was found between the external transcribed spacer and the terminal non-transcribed spacer of Physarum polycephalum rDNA. The homologous sequences were located 2kb upstream from the...
Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum.
Nomiyama, H, Sakaki, Y, Takagi, Y
The Physarum polycephalum 26S ribosomal RNA gene contains two intervening sequences (introns). The DNA sequence of one of these introns was analyzed together with that of its flanking regions...
Nucleotide sequence of Physarum polycephalum 5.8S rRNA gene and its flanking regions.
Otsuka, T, Nomiyama, H, Sakaki, Y, Takagi, Y
The nucleotide sequence of Physarum polycephalum 5.8S rRNA gene and its flanking regions has been determined. The homologies of the 5.8S rRNA sequence with those of Saccharomyces, Chlamydomonas and...
GENAS: a database system for nucleic acid sequence analysis.
Kuhara, S, Matsuo, F, Futamura, S, Fujita, A, Shinohara, T, Takagi, T, ...
A database system, named GENAS (GENe Analyzing System), for computer analysis of sequence was constructed using Adbis which is a relational database management system (1). GENAS enables us to...
Sequence analysis of a KpnI family member near the 3' end of human beta-globin gene.
Hattori, M, Hidaka, S, Sakaki, Y
We determined the complete nucleotide sequence (6125 bp) of a full-length member of human KpnI family, designated T beta G41, which is located about 3 kb downstream from the beta-globin gene....
Some KpnI family members are associated with the Alu family in the human genome.
Miyake, T, Migita, K, Sakaki, Y
The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5'...
Nomiyama, H, Kuhara, S, Kukita, T, Otsuka, T, Sakaki, Y
The 26S ribosomal RNA gene of Physarum polycephalum is interrupted by two introns, and we have previously determined the sequence of one of them (intron 1) (Nomiyama et al. Proc.Natl.Acad.Sci.USA 78,...
Hohjoh, H, Minakami, R, Sakaki, Y
L1 (LINE-1), a long interspersed repetitive DNA family of mammalian genomes, is thought to be a sequence family derived from a retrotransposon-like element(s), but its actively transposable unit(s)...
Ito, T, Tanahashi, H, Misumi, Y, Sakaki, Y
During acute inflammation, a group of liver-derived plasma proteins, acute phase proteins (APPs), increase in concentration. Interleukin-6 (IL-6) is responsible for this increase via the induction of...
A novel procedure for selective cloning of NotI linking fragments from mammalian genomes.
A novel procedure has been developed for selective cloning of NotI linking fragments from mammalian genomes. Since the majority of the NotI sites in mammalian genomes are considered to be localized...
Fujita, A, Hattori, M, Takenaka, O, Sakaki, Y
We previously reported that some L1 family (KpnI family) members are closely associated with the Alu family sequence. To understand the details of the L1-Alu association, the structure of a L1-Alu...
A bacteriophage (phiYS40) infectious to an extreme thermophile, Thermus thermophilus HB8, was isolated and characterized. phiYS40 grows over the temperature range of 56 to 78 C, and the optimum...
Otsuka, T, Nomiyama, H, Yoshida, H, Kukita, T, Kuhara, S, Sakaki, Y
The complete nucleotide sequences of the 5.8S and 26S rRNA genes of Physarum polycephalum and the transcribed spacer between them were determined. Comparison of the sequences with those of the...
Cloning and sequence analysis of cDNA for the luminescent protein aequorin.
Inouye, S, Noguchi, M, Sakaki, Y, Takagi, Y, Miyata, T, Iwanaga, S, ...
The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. A cDNA library of Aequorea was...
Structure of Met30 variant of transthyretin and its amyloidogenic implications.
Terry, C J, Damas, A M, Oliveira, P, Saraiva, M J, Alves, I L, Costa, P P, ...
Familial amyloidotic polyneuropathy (FAP) is an autosomal dominant hereditary type of lethal amyloidosis involving single (or double) amino acid substitutions in the amyloidogenic protein...
Furuya, H, Yoshioka, K, Sasaki, H, Sakaki, Y, Nakazato, M, Matsuo, H, ...
A Japanese family with atypical type I familial amyloidotic polyneuropathy (FAP) in Iiyama, Japan was studied. Most of the family members have dysfunctions in the central nervous system, in addition...
Primary structure of rat liver alkaline phosphatase deduced from its cDNA.
Misumi, Y, Tashiro, K, Hattori, M, Sakaki, Y, Ikehara, Y
Rat liver alkaline phosphatase (ALP) was markedly induced by treatment of rats by bile-duct ligation and colchicine injection. Taking this advantage for enrichment of ALP mRNA, we constructed a...
Iwaki, T., Iwaki, A., Tateishi, J., Sakaki, Y., Goldman, J. E.
To understand the significance of the accumulation of alpha B-crystallin in Rosenthal fibers within astrocytes, the expression and metabolism of alpha B-crystallin in glioma cell lines were examined...