Y. Yoshikai

Protective Roles of γδ T Cells and Interleukin-15 in Escherichia coli Infection in Mice

Takano, M., Nishimura, H., Kimura, Y., Mokuno, Y., Washizu, J., Itohara, S., ...

The number of γδ T cells in the peritoneal cavity was increased after an intraperitoneal (i.p.) infection with Escherichia coli in lipopolysaccharide (LPS)-responsive C3H/HeN mice but not in...

Increased susceptibility to primary infection with Listeria monocytogenes in germfree mice may be due to lack of accumulation of L-selectin+ CD44+ T cells in sites of inflammation.

Inagaki, H, Suzuki, T, Nomoto, K, Yoshikai, Y

The host defense of germfree (GF) mice against primary infection with Listeria monocytogenes was compared with that of specific-pathogen-free (SPF) mice. In SPF mice, the numbers of bacteria in the...

CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis.

Fuse, Y, Nishimura, H, Maeda, K, Yoshikai, Y

CD95 (Fas) is known to mediate activation-induced T-cell death by apoptosis. To understand the role of CD95 during the course of bacterial infection, we examined the kinetics of alphabeta and...

Prevention of indigenous infection of mice with Escherichia coli by nonspecific immunostimulation.

Nomoto, K, Yokokura, T, Mitsuyama, M, Yoshikai, Y, Nomoto, K

We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an intestinal infection with indigenous Escherichia coli induced by the drug (K....

A novel V beta 2-specific endogenous mouse mammary tumor virus which is capable of producing a milk-borne exogenous virus.

Niimi, N, Wajjwalku, W, Ando, Y, Nakamura, N, Ueda, M, Yoshikai, Y

We have previously reported new Mtv loci, Mtv-48 and -51, in the Japanese laboratory mouse strains CS and NC. Here we show by backcross analysis that both Mtv-48 and -51 cosegregate with very slow...

Apoptosis by CD95 (Fas)-dependent and -independent mechanisms in Peyer's patch lymphocytes in murine retrovirus-induced immunodeficiency syndrome.

Usami, J, Hiromatsu, K, Aoki, Y, Kobayashi, N, Makino, M, Yagita, H, ...

CD95 (Fas)/CD95 ligand (CD95 L)-mediated apoptosis is thought to be involved in the delayed progression of murine AIDS (MAIDS) induced by LP-BM5 murine leukemia virus (MuLV). We show evidence of...

Clonal expansion of superantigen-reactive T cells is resistant to FK506 in mice with AIDS.

Yoshikai, Y, Kidokoro, H, Kimura, K, Aoki, Y, Makino, M, Hiromatsu, K

Subcutaneous injection of FK506 (10 mg/kg of body weight) completely blocked the clonal expansion of staphylococcal enterotoxin A (SEA)-reactive T cells in healthy (control) mice after SEA injection...

Sendai virus pneumonia: evidence for the early recruitment of gamma delta T cells during the disease course.

Ogasawara, T, Emoto, M, Kiyotani, K, Shimokata, K, Yoshida, T, Nagai, Y, ...

We previously reported that gamma delta T cells appeared and could play a protective role early in infections with intracellular bacteria such as Listeria monocytogenes, Mycobacterium bovis BCG, and...

Age-associated increase in the expression of T-cell antigen receptor gamma-chain gene in conventional and germfree mice.

Yoshikai, Y, Matsuzaki, G, Kishihara, K, Nomoto, K, Yokokura, T, Nomoto, K

To determine whether environmental antigens such as intestinal microflora contribute to expansion of the T-cell repertoire, age-related changes in the expression level of T-cell antigen receptor...

Protective effect of Lactobacillus casei on Pseudomonas aeruginosa infection in mice.

Miake, S, Nomoto, K, Yokokura, T, Yoshikai, Y, Mutai, M, Nomoto, K

The protective effect of heat-killed Lactobacillus casei YIT9018 (LC 9018) against Pseudomonas aeruginosa infection in mice was compared with that of Corynebacterium parvum. Survival of mice after...

Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor beta chain.

Toyonaga, B, Yoshikai, Y, Vadasz, V, Chin, B, Mak, T W

The organization and sequences of the human beta-chain T-cell receptor diversity, joining, and constant region segments are described. The beta chain of the human T-cell receptor, analogous to the...

Predominant activation and expansion of V gamma 9-bearing gamma delta T cells in vivo as well as in vitro in Salmonella infection.

Hara, T, Mizuno, Y, Takaki, K, Takada, H, Akeda, H, Aoki, T, ...

Gamma delta T cell receptor-positive cells (gamma delta T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on gamma delta T cells in...

Protective Roles of γδ T Cells and Interleukin-15 in Escherichia coli Infection in Mice

Takano, M., Nishimura, H., Kimura, Y., Mokuno, Y., Washizu, J., Itohara, S., ...

The number of γδ T cells in the peritoneal cavity was increased after an intraperitoneal (i.p.) infection with Escherichia coli in lipopolysaccharide (LPS)-responsive C3H/HeN mice but not in...

Increased susceptibility to primary infection with Listeria monocytogenes in germfree mice may be due to lack of accumulation of L-selectin+ CD44+ T cells in sites of inflammation.

Inagaki, H, Suzuki, T, Nomoto, K, Yoshikai, Y

The host defense of germfree (GF) mice against primary infection with Listeria monocytogenes was compared with that of specific-pathogen-free (SPF) mice. In SPF mice, the numbers of bacteria in the...

CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis.

Fuse, Y, Nishimura, H, Maeda, K, Yoshikai, Y

CD95 (Fas) is known to mediate activation-induced T-cell death by apoptosis. To understand the role of CD95 during the course of bacterial infection, we examined the kinetics of alphabeta and...

Prevention of indigenous infection of mice with Escherichia coli by nonspecific immunostimulation.

Nomoto, K, Yokokura, T, Mitsuyama, M, Yoshikai, Y, Nomoto, K

We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an intestinal infection with indigenous Escherichia coli induced by the drug (K....

A novel V beta 2-specific endogenous mouse mammary tumor virus which is capable of producing a milk-borne exogenous virus.

Niimi, N, Wajjwalku, W, Ando, Y, Nakamura, N, Ueda, M, Yoshikai, Y

We have previously reported new Mtv loci, Mtv-48 and -51, in the Japanese laboratory mouse strains CS and NC. Here we show by backcross analysis that both Mtv-48 and -51 cosegregate with very slow...

Apoptosis by CD95 (Fas)-dependent and -independent mechanisms in Peyer's patch lymphocytes in murine retrovirus-induced immunodeficiency syndrome.

Usami, J, Hiromatsu, K, Aoki, Y, Kobayashi, N, Makino, M, Yagita, H, ...

CD95 (Fas)/CD95 ligand (CD95 L)-mediated apoptosis is thought to be involved in the delayed progression of murine AIDS (MAIDS) induced by LP-BM5 murine leukemia virus (MuLV). We show evidence of...

Clonal expansion of superantigen-reactive T cells is resistant to FK506 in mice with AIDS.

Yoshikai, Y, Kidokoro, H, Kimura, K, Aoki, Y, Makino, M, Hiromatsu, K

Subcutaneous injection of FK506 (10 mg/kg of body weight) completely blocked the clonal expansion of staphylococcal enterotoxin A (SEA)-reactive T cells in healthy (control) mice after SEA injection...

Sendai virus pneumonia: evidence for the early recruitment of gamma delta T cells during the disease course.

Ogasawara, T, Emoto, M, Kiyotani, K, Shimokata, K, Yoshida, T, Nagai, Y, ...

We previously reported that gamma delta T cells appeared and could play a protective role early in infections with intracellular bacteria such as Listeria monocytogenes, Mycobacterium bovis BCG, and...

Age-associated increase in the expression of T-cell antigen receptor gamma-chain gene in conventional and germfree mice.

Yoshikai, Y, Matsuzaki, G, Kishihara, K, Nomoto, K, Yokokura, T, Nomoto, K

To determine whether environmental antigens such as intestinal microflora contribute to expansion of the T-cell repertoire, age-related changes in the expression level of T-cell antigen receptor...

Protective effect of Lactobacillus casei on Pseudomonas aeruginosa infection in mice.

Miake, S, Nomoto, K, Yokokura, T, Yoshikai, Y, Mutai, M, Nomoto, K

The protective effect of heat-killed Lactobacillus casei YIT9018 (LC 9018) against Pseudomonas aeruginosa infection in mice was compared with that of Corynebacterium parvum. Survival of mice after...

Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor beta chain.

Toyonaga, B, Yoshikai, Y, Vadasz, V, Chin, B, Mak, T W

The organization and sequences of the human beta-chain T-cell receptor diversity, joining, and constant region segments are described. The beta chain of the human T-cell receptor, analogous to the...

Predominant activation and expansion of V gamma 9-bearing gamma delta T cells in vivo as well as in vitro in Salmonella infection.

Hara, T, Mizuno, Y, Takaki, K, Takada, H, Akeda, H, Aoki, T, ...

Gamma delta T cell receptor-positive cells (gamma delta T cells) have recently been implicated to play a role in the protection against infectious pathogens. Serial studies on gamma delta T cells in...

Effects of in vivo administration of anti-IL-10 monoclonal antibody on the host defence mechanism against murine Salmonella infection.

Arai, T, Hiromatsu, K, Nishimura, H, Kimura, Y, Kobayashi, N, Ishida, H, ...

Interleukin-10 (IL-10) is a cytokine that regulates various macrophage functions. To elucidate the involvement of endogenous IL-10 in the early stage of murine salmonellosis, we examined the effect...

Evidence for the early recruitment of T-cell receptor gamma delta+ T cells during rat listeriosis.

Kimura, Y, Tomida, S, Matsumoto, Y, Hiromatsu, K, Yoshikai, Y

We have previously reported that heat-shock protein (hsp) 60-reactive T-cell receptor (TCR)gamma delta+ T cells appear in the peritoneal cavity during the early stage of infection with Listeria...

A protective role of gamma delta T cells in primary infection with Listeria monocytogenes in autoimmune non-obese diabetic mice.

Usami, J, Hiromatsu, K, Matsumoto, Y, Maeda, K, Inagaki, H, Suzuki, T, ...

We investigated the host defense mechanism in primary infection with Listeria monocytogenes in non-obese diabetic (NOD) mice at pre-diabetic stage showing an impaired responsiveness of the alpha beta...

Conversion of Salmonella typhimurium to L-forms contributes to the maintenance of acquired immunity against murine typhoid.

Kita, E, Emoto, M, Nishikawa, F, Yoshikai, Y, Kashiba, S

Conversion of Salmonella typhimurium to L-forms, both in vitro and in vivo, resulted in the expression of proteins cross-reacting to the mycobacterial 65,000 MW heat-shock protein (hsp). Immunization...

An increase in number of T-cell receptor gamma/delta-bearing T cells in athymic nude mice treated with complete Freund's adjuvants.

Yoshikai, Y, Matsuzaki, G, Inoue, T, Nomoto, K

It has been reported previously that environmental antigens such as intestinal microflora play an important role in an age-associated increase in number of T-cell receptor gamma/delta-bearing T...

Different expression of T-cell receptor beta-chain variable region genes in lymph nodes of lpr mice with different alleles of the major histocompatibility complex.

Ohga, S, Yoshikai, Y, Kishihara, K, Matsuzaki, G, Ogimoto, M, Nomoto, K

In order to search for a possible role of abnormally proliferating T cells in developing autoimmune disease in lpr mice, and to define the difference of the T cells among various lpr-congeneic mice...

Two differential pathways from double-negative to double-positive thymocytes.

Matsumoto, K, Yoshikai, Y, Moroi, Y, Asano, T, Ando, T, Nomoto, K

Murine thymocytes are divided into four major populations on the basis of expression of CD4 and CD8 antigens. The bulk of evidence favours the view that CD4-CD8- cells can develop into CD4-CD8+ and...

Effect of recombinant human granulocyte colony-stimulating factor (rh G-CSF) on murine resistance against Listeria monocytogenes.

Serushago, B A, Yoshikai, Y, Handa, T, Mitsuyama, M, Muramori, K, Nomoto, K

Recombinant human granulocyte colony-stimulating factor (rh G-CSF) enhanced resistance of mice against Listeria monocytogenes (LM) as determined by survival and bacterial growth. Mice pretreated with...

Sequential appearance of T-cell receptor gamma delta- and alpha beta-bearing intestinal intra-epithelial lymphocytes in mice after irradiation.

Yoshikai, Y, Ishida, A, Murosaki, S, Ando, T, Nomoto, K

We have previously reported that T-cell receptor (TcR) gamma delta-bearing T cells precede TcR alpha beta-bearing T cells in appearance in the thymus after whole-body irradiation. In the present...

The stage of negative selection in tolerance induction in neonatal mice.

Ando, T, Yoshikai, Y, Matsuzaki, G, Takimoto, H, Nomoto, K

In flow microfluorometry analysis of thymus and lymph node cells of C57BL/6(I-E-,Mlsb) mice rendered neonatally tolerant to (C57BL/6 x AKR/J)F1 (I-E+,Mlsb/a) lymphoid cells, both CD4+ and CD8+ cells...

Rearrangements of T-cell antigen receptor gamma and delta chain genes are detected in the long-term cultured bone marrow cells of athymic nude mice but not in those of euthymic mice.

Yoshikai, Y, Takeda, Y, Ohga, S, Kishihara, K, Matsuzaki, G, Nomoto, K

We have previously shown that extrathymic rearrangements of T-cell receptor (TcR) gamma and delta chain genes occur in the peripheral lymphoid tissues of athymic nude mice. To further determine where...

Abnormal rearrangements of T-cell receptor genes occur in long-term cultured bone marrow cells of lpr/lpr mice.

Ohga, S, Yoshikai, Y, Matsumoto, K, Kishihara, K, Matsuzaki, G, Nomoto, K

To search the abnormality in prethymic T-cell precursors in lpr/lpr(lpr) mice, rearrangement and expression of T-cell receptor (TcR) genes were investigated in long-term cultured bone marrow (LTBM)...

Clonal deletion of self-Mls-reactive thymocytes at the early stage in H-2-compatible but Mls-disparate radiation chimeras.

Ogimoto, M, Yoshikai, Y, Matsuzaki, G, Ohga, S, Matsumoto, K, Nomoto, K

Clonal deletion of T cells capable of recognizing both host-type Mls and donor-type Mls occurred in the peripheral mature T-cell pool in radiation bone marrow chimeras of two H-2-compatible...

T cells expressing both L-selectin and CD44 molecules increase in number in peritoneal exudate cells and in vitro-stimulated spleen cells from mice immunized intraperitoneally with Listeria monocytogenes.

Li, X Y, Matsuzaki, G, Yoshikai, Y, Muramori, K, Nomoto, K

L-selectin, which was first reported as MEL-14 antigen in mice, is a type of animal lectin and expressed on lymphocytes, neutrophils and macrophages. L-selectin has been reported to be a homing...

Characterization of T-cell receptor gamma delta T cells appearing at the early phase of murine Listeria monocytogenes infection.

Matsuzaki, G, Hiromatsu, K, Yoshikai, Y, Muramori, K, Nomoto, K

It has been shown that T-cell receptor (TcR) gamma delta + CD4- CD8- T cells increase in number and have an important role in early protection in murine Listeria monocytogenes infection. In this...

A protective role of extrathymic alpha beta TcR cells in the liver in primary murine salmonellosis.

Matsumoto, Y, Emoto, M, Usami, J, Maeda, K, Yoshikai, Y

The liver comprises unique T cells differentiating extrathymically and expressing an intermediate intensity of alpha beta T-cell receptor (TcR) and a high intensity of leucocyte function antigen-1...

Inhibition of allograft rejection by anti-T-cell receptor-alpha beta monoclonal antibodies preserving resistance to bacterial infection.

Eto, M, Yoshikai, Y, Nishimura, Y, Hiromatsu, K, Maeda, T, Nomoto, K, ...

Anti-CD3 monoclonal antibody (mAb) has been administered in clinical organ transplantation to reverse acute allograft rejection; however, severe immunodeficiency can result from such mAb treatment...

V delta 5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity.

Kobayashi, N, Matsuzaki, G, Yoshikai, Y, Seki, R, Ivanyi, J, Nomoto, K

The purpose of this paper was to study the gamma delta T-cell receptor repertoire and target specificity following stimulation of peripheral T cells of BALB/c mice with autologous or bacterial...

Protective immunity to Listeria monocytogenes in neonatally thymectomized (NTx) mice: involvement of T cells distinct from those in sham-thymectomized mice.

Watanabe, Y, Mitsuyama, M, Koga, T, Handa, T, Yoshikai, Y, Nomoto, K

Neonatally thymectomized (NTx) mice, whose ability to mount antigen-specific cell-mediated immunity is reported to be generally defective, were found to be capable of mounting a normal level of...

Th0-like CD4+ T cells protect mice with murine retrovirus-induced immunodeficiency syndrome (MAIDS) against co-infection with Listeria monocytogenes.

Hiromatsu, K, Nishimura, H, Kimura, K, Aoki, Y, Usami, J, Kobayashi, N, ...

We examined the host defence mechanism against infection with Listeria monocytogenes, a facultative intracellular bacterium, in mice with murine acquired immunodeficiency syndrome (MAIDS) caused by...

Effect of stimulation and blockade of mononuclear phagocyte system on the delayed footpad reaction to SRBC in mice.

Yoshikai, Y, Miake, S, Matsumoto, T, Nomoto, K, Takeya, K

The delayed footpad reaction to sheep erythrocytes (SRBC) was studied in mice whose mononuclear phagocyte system (MPS) was blocked or stimulated. Colloidal carbon or carrageenan was used for the...

Relationship between non-specific activity of macrophages and immune responses to Listeria monocytogenes.

Yoshikai, Y, Miake, S, Matsumoto, T, Nomoto, K, Takeya, K

Delayed footpad reactions and acquired cellular resistance to Listeria monocytogenes were studied in mice whose mononuclear phagocyte system (MPS) had been blocked or stimulated. Colloidal carbon was...

Effect of stimulation and blockade of mononuclear phagocyte system on the induction of suppressor T cells of delayed footpad reaction to SRBC in mice.

Yoshikai, Y, Miake, S, Matsumoto, T, Nomoto, K, Takeya, K

The role of mononuclear phagocyte system (MPS) in the induction of suppressor T cells which depress the delayed footpad reaction to sheep erythrocytes (SRBC) was studied in mice in which MPS was...

Thymus-dependent modulation of Ly49 inhibitory receptor expression on NK1.1+γ/δ T cells

Hara, T, Nishimura, H, Hasegawa, Y, Yoshikai, Y

Major histocompatibility complex (MHC) class I-specific inhibitory receptors are expressed not only on natural killer (NK) cells but also on some subsets of T cells. We here show Ly49 expression on...

Subpopulations of CD4+ cells in lpr/lpr mice: differences in expression of T cell receptor/CD3 complex and proliferative responses.

Asano, T, Yoshikai, Y, Matsumoto, K, Matuzaki, G, Nomoto, K

CD4+ cells from autoimmune-prone C57BL/6 lpr/lpr mice contain two subpopulations, B220-CD4+ and B220+CD4+ cells. Highly purified B220-CD4+ cells from C57BL/6 +/+ and lpr/lpr mice were examined by...

Expression and sequences of T cell receptor beta-chain variable genes in the enlarged lymph nodes of C57BL/6-lpr/lpr mice.

Ohga, S, Yoshikai, Y, Kishihara, K, Matsuzaki, G, Asano, T, Nomoto, K

An autosomal recessive gene, lpr, is responsible for lymphoproliferation and autoimmunity of lpr-mice, in which background genes are also known to influence the development of autoimmune disease. To...

Novel roles of osteopontin and CXC chemokine ligand 7 in the defence against mycobacterial infection

Khajoee, V, Saito, M, Takada, H, Nomura, A, Kusuhara, K, Yoshida, S-I, ...

Granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced human monocyte-derived macrophage (GM-Mφ) or macrophage CSF (M-CSF)-induced human monocyte-derived Mφ (M-Mφ) are distinct in terms...

Serum levels of IL-10, IL-15 and soluble tumour necrosis factor-alpha (TNF-α) receptors in type C chronic liver disease

KAKUMU, S, OKUMURA, A, ISHIKAWA, T, YANO, M, ENOMOTO, A, NISHIMURA, H, ...

We previously reported that the number of TNF-α-producing cells was increased in the liver of patients with type C chronic liver disease. To understand further the pathophysiology of this change, we...

Interleukin-15 production at the early stage after oral infection with Listeria monocytogenes in mice

MITANI, A, NISHIMURA, H, HIROSE, K, WASHIZU, J, KIMURA, Y, TANAKA, S, ...

We previously reported that exogenous interleukin-15 (IL-15) induces proliferation and activation of intestinal intraepithelial lymphocytes (i-IEL) in naive mice. To investigate the ability of...

Vγ1+ γδ T cells play protective roles at an early phase of murine cytomegalovirus infection through production of interferon-γ

Ninomiya, T, Takimoto, H, Matsuzaki, G, Hamano, S, Yoshida, H, Yoshikai, Y, ...

Cytomegalovirus (CMV) causes severe opportunistic infection in immunocompromised hosts. The importance of conventional αβ T cells in protection against CMV infection has been well documented....