High-resolution DNA-binding specificity analysis of yeast transcription factors (2009)
Zhu, Cong, McCord, Rachel Patton, Shi, Zhenwei, Berger, Michael F., Newburger, Daniel E., ...
Transcription factors (TFs) regulate the expression of genes through sequence-specific interactions with DNA-binding sites. However, despite recent progress in identifying in vivo TF binding sites by...
Taycher, Elena, Rolfs, Andreas, Hu, Yanhui, Zuo, Dongmei, Mohr, Stephanie E, Williamson, Janice, ...
Abstract Background Whereas the molecular assembly of protein expression clones is readily automated and routinely accomplished in high throughput, sequence verification of these clones is still...
Hu, Yanhui, Rolfs, Andreas, Bhullar, Bhupinder, Murthy, Tellamraju V. S., Zhu, Cong, Berger, Michael F., ...
The availability of an annotated genome sequence for the yeast Saccharomyces cerevisiae has made possible the proteome-scale study of protein function and protein–protein interactions. These...
PlasmID: a centralized repository for plasmid clone information and distribution (2007)
Zuo, Dongmei, Mohr, Stephanie E., Hu, Yanhui, Taycher, Elena, Rolfs, Andreas, Kramer, Jason, ...
The Plasmid Information Database (PlasmID; http://plasmid.hms.harvard.edu) was developed as a community-based resource portal to facilitate search and request of plasmid clones shared with the...
Hu, Yanhui, Rolfs, Andreas, Bhullar, Bhupinder, Murthy, Tellamraju V. S., Zhu, Cong, Berger, Michael F., ...
The availability of an annotated genome sequence for the yeast Saccharomyces cerevisiae has made possible the proteome-scale study of protein function and protein–protein interactions. These...
Bmc Bioinformatics, Elena Taycher, Andreas Rolfs, Yanhui Hu, Dongmei Zuo, Stephanie E Mohr, ...
Software A novel approach to sequence validating protein expression clones with automated decision making
Nonaqueous phase liquids (NAPLs) introduced into an aquifer can cause long-term contamination. While some of the NAPL may be recoverable, most will dissolve into the surrounding water over a period...
During muscle differentiation in vertebrates, myoblasts initially form in somites and then migrate to proper locations in the trunk and limbs. Once there, these cells are faced with one of three...
During muscle differentiation in vertebrates, myoblasts initially form in somites and then migrate to proper locations in the trunk and limbs. Once there, these cells are faced with one of three...
Hu, Yanhui, Cascone, Pamela J., Cheng, Lihong, Sun, Danhui, Nambu, John R., Schwartz, Lawrence M.
During myogenesis, reductions in trophic factor availability signal most myoblasts to fuse, up-regulate the expression of muscle-specific genes, and form myotubes. Those cells failing to...
Proteome-scale purification of human proteins from bacteria
Braun, Pascal, Hu, Yanhui, Shen, Binghua, Halleck, Allison, Koundinya, Malvika, Harlow, Ed, ...
The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps...
Park, Jaehong, Hu, Yanhui, Murthy, T. V. S., Vannberg, Fredrik, Shen, Binghua, Rolfs, Andreas, ...
Kinases catalyze the phosphorylation of proteins, lipids, sugars, nucleosides, and other important cellular metabolites and play key regulatory roles in all aspects of eukaryotic cell physiology....
Hu, Yanhui, Cascone, Pamela J., Cheng, Lihong, Sun, Danhui, Nambu, John R., Schwartz, Lawrence M.
During myogenesis, reductions in trophic factor availability signal most myoblasts to fuse, up-regulate the expression of muscle-specific genes, and form myotubes. Those cells failing to...
Proteome-scale purification of human proteins from bacteria
Braun, Pascal, Hu, Yanhui, Shen, Binghua, Halleck, Allison, Koundinya, Malvika, Harlow, Ed, ...
The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps...
Park, Jaehong, Hu, Yanhui, Murthy, T. V. S., Vannberg, Fredrik, Shen, Binghua, Rolfs, Andreas, ...
Kinases catalyze the phosphorylation of proteins, lipids, sugars, nucleosides, and other important cellular metabolites and play key regulatory roles in all aspects of eukaryotic cell physiology....
PlasmID: a centralized repository for plasmid clone information and distribution
Zuo, Dongmei, Mohr, Stephanie E., Hu, Yanhui, Taycher, Elena, Rolfs, Andreas, Kramer, Jason, ...
The Plasmid Information Database (PlasmID; ) was developed as a community-based resource portal to facilitate search and request of plasmid clones shared with the Dana-Farber/Harvard Cancer Center...
A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis
Murthy, Tal, Rolfs, Andreas, Hu, Yanhui, Shi, Zhenwei, Raphael, Jacob, Moreira, Donna, ...
The rapid development of new technologies for the high throughput (HT) study of proteins has increased the demand for comprehensive plasmid clone resources that support protein expression. These...
A novel approach to sequence validating protein expression clones with automated decision making
Taycher, Elena, Rolfs, Andreas, Hu, Yanhui, Zuo, Dongmei, Mohr, Stephanie E, Williamson, Janice, ...
Hu, Yanhui, Rolfs, Andreas, Bhullar, Bhupinder, Murthy, Tellamraju V. S., Zhu, Cong, Berger, Michael F., ...
The availability of an annotated genome sequence for the yeast Saccharomyces cerevisiae has made possible the proteome-scale study of protein function and protein–protein interactions. These...
A Biomedically Enriched Collection of 7000 Human ORF Clones
Rolfs, Andreas, Hu, Yanhui, Ebert, Lars, Hoffmann, Dietmar, Zuo, Dongmei, Ramachandran, Niro, ...
We report the production and availability of over 7000 fully sequence verified plasmid ORF clones representing over 3400 unique human genes. These ORF clones were derived using the human MGC...
Rolfs, Andreas, Montor, Wagner R., Yoon, Sang Sun, Hu, Yanhui, Bhullar, Bhupinder, Kelley, Fontina, ...
Cholera, an infectious disease with global impact, is caused by pathogenic strains of the bacterium Vibrio cholerae. High-throughput functional proteomics technologies now offer the opportunity to...
Kinase requirements in human cells: I. Comparing kinase requirements across various cell types
Grueneberg, Dorre A., Degot, Sebastien, Pearlberg, Joseph, Li, Wenliang, Davies, Joan E., Baldwin, Amy, ...
shRNA loss-of-function screens were used to identify kinases that were rate-limiting for promoting cell proliferation and survival. Here, we study the differences in kinase requirements among various...
High-resolution DNA-binding specificity analysis of yeast transcription factors
Zhu, Cong, McCord, Rachel Patton, Shi, Zhenwei, Berger, Michael F., Newburger, Daniel E., ...
Transcription factors (TFs) regulate the expression of genes through sequence-specific interactions with DNA-binding sites. However, despite recent progress in identifying in vivo TF binding sites by...