Yaoping Zhang

Whole-genome shotgun optical mapping of rhodospirillum rubrum (2004)

Reslewic, Susan, Zhou, Shiguo, Place, Mike, Zhang, Yaoping, Briska, Adam, Goldstein, Steve, ...

Rhodospirillum rubrum is a phototrophic purple non-sulfur bacterium known for its unique and well-studied nitrogen fixation and carbon monoxide oxidation systems, and as a source of hydrogen and...

Correlation of Activity Regulation and Substrate Recognition of the ADP-Ribosyltransferase That Regulates Nitrogenase Activity in Rhodospirillum rubrum

Kim, Kitai, Zhang, Yaoping, Roberts, Gary P.

In Rhodospirillum rubrum, nitrogenase activity is regulated posttranslationally through the ADP-ribosylation of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase (DRAT)....

Mutagenesis and Functional Characterization of the glnB, glnA, and nifA Genes from the Photosynthetic Bacterium Rhodospirillum rubrum

Zhang, Yaoping, Pohlmann, Edward L., Ludden, Paul W., Roberts, Gary P.

Nitrogen fixation is tightly regulated in Rhodospirillum rubrum at two different levels: transcriptional regulation of nif expression and posttranslational regulation of dinitrogenase reductase by...

Effects of Perturbations of the Nitrogenase Electron Transfer Chain on Reversible ADP-Ribosylation of Nitrogenase Fe Protein in Klebsiella pneumoniae Strains Bearing the Rhodospirillum rubrum dra Operon

Halbleib, Cale M., Zhang, Yaoping, Roberts, Gary P., Ludden, Paul W.

The redox state of nitrogenase Fe protein is shown to affect regulation of ADP-ribosylation in Klebsiella pneumoniae strains transformed by plasmids carrying dra genes from Rhodospirillum rubrum. The...

Effect of PII and Its Homolog GlnK on Reversible ADP-Ribosylation of Dinitrogenase Reductase by Heterologous Expression of the Rhodospirillum rubrum Dinitrogenase Reductase ADP-Ribosyl Transferase–Dinitrogenase Reductase-Activating Glycohydrolase Regulatory System in Klebsiella pneumoniae

Zhang, Yaoping, Pohlmann, Edward L., Halbleib, Cale M., Ludden, Paul W., Roberts, Gary P.

Reversible ADP-ribosylation of dinitrogenase reductase, catalyzed by the dinitrogenase reductase ADP-ribosyl transferase–dinitrogenase reductase-activating glycohydrolase (DRAT-DRAG) regulatory...

Effects of Specific Amino Acid Substitutions on Activities of Dinitrogenase Reductase-Activating Glycohydrolase from Rhodospirillum rubrum

Antharavally, Babu S., Poyner, Russell R., Zhang, Yaoping, Roberts, Gary P., Ludden, Paul W.

Site-directed mutagenesis of the draG gene was used to generate altered forms of dinitrogenase reductase-activating glycohydrolase (DRAG) with D123A, H142L, H158N, D243G, and E279R substitutions. The...

Functional Characterization of Three GlnB Homologs in the Photosynthetic Bacterium Rhodospirillum rubrum: Roles in Sensing Ammonium and Energy Status

Zhang, Yaoping, Pohlmann, Edward L., Ludden, Paul W., Roberts, Gary P.

The GlnB (PII) protein, the product of glnB, has been characterized previously in the photosynthetic bacterium Rhodospirillum rubrum. Here we describe identification of two other PII homologs in this...

Identification of critical residues in GlnB for its activation of NifA activity in the photosynthetic bacterium Rhodospirillum rubrum

Zhang, Yaoping, Pohlmann, Edward L., Roberts, Gary P.

The PII regulatory protein family is unusually widely distributed, being found in all three domains of life. Three PII homologs called GlnB, GlnK, and GlnJ have been identified in the photosynthetic...

GlnD Is Essential for NifA Activation, NtrB/NtrC-Regulated Gene Expression, and Posttranslational Regulation of Nitrogenase Activity in the Photosynthetic, Nitrogen-Fixing Bacterium Rhodospirillum rubrum

Zhang, Yaoping, Pohlmann, Edward L., Roberts, Gary P.

GlnD is a bifunctional uridylyltransferase/uridylyl-removing enzyme and is thought to be the primary sensor of nitrogen status in the cell. It plays an important role in nitrogen assimilation and...

Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

Reslewic, Susan, Zhou, Shiguo, Place, Mike, Zhang, Yaoping, Briska, Adam, Goldstein, Steve, ...

Rhodospirillum rubrum is a phototrophic purple nonsulfur bacterium known for its unique and well-studied nitrogen fixation and carbon monoxide oxidation systems and as a source of hydrogen and...

Identification of Rhodospirillum rubrum GlnB Variants That Are Altered in Their Ability To Interact with Different Targets in Response to Nitrogen Status Signals

Zhu, Yu, Conrad, Mary C., Zhang, Yaoping, Roberts, Gary P.

In Rhodospirillum rubrum, NifA, the transcriptional activator for the nif genes, is posttranslationally activated only by the uridylylated form of GlnB, one of three PII homologs in the organism. We...

Correlation of Activity Regulation and Substrate Recognition of the ADP-Ribosyltransferase That Regulates Nitrogenase Activity in Rhodospirillum rubrum

Kim, Kitai, Zhang, Yaoping, Roberts, Gary P.

In Rhodospirillum rubrum, nitrogenase activity is regulated posttranslationally through the ADP-ribosylation of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase (DRAT)....

Mutagenesis and Functional Characterization of the glnB, glnA, and nifA Genes from the Photosynthetic Bacterium Rhodospirillum rubrum

Zhang, Yaoping, Pohlmann, Edward L., Ludden, Paul W., Roberts, Gary P.

Nitrogen fixation is tightly regulated in Rhodospirillum rubrum at two different levels: transcriptional regulation of nif expression and posttranslational regulation of dinitrogenase reductase by...

Effects of Perturbations of the Nitrogenase Electron Transfer Chain on Reversible ADP-Ribosylation of Nitrogenase Fe Protein in Klebsiella pneumoniae Strains Bearing the Rhodospirillum rubrum dra Operon

Halbleib, Cale M., Zhang, Yaoping, Roberts, Gary P., Ludden, Paul W.

The redox state of nitrogenase Fe protein is shown to affect regulation of ADP-ribosylation in Klebsiella pneumoniae strains transformed by plasmids carrying dra genes from Rhodospirillum rubrum. The...

Effect of PII and Its Homolog GlnK on Reversible ADP-Ribosylation of Dinitrogenase Reductase by Heterologous Expression of the Rhodospirillum rubrum Dinitrogenase Reductase ADP-Ribosyl Transferase–Dinitrogenase Reductase-Activating Glycohydrolase Regulatory System in Klebsiella pneumoniae

Zhang, Yaoping, Pohlmann, Edward L., Halbleib, Cale M., Ludden, Paul W., Roberts, Gary P.

Reversible ADP-ribosylation of dinitrogenase reductase, catalyzed by the dinitrogenase reductase ADP-ribosyl transferase–dinitrogenase reductase-activating glycohydrolase (DRAT-DRAG) regulatory...

Effects of Specific Amino Acid Substitutions on Activities of Dinitrogenase Reductase-Activating Glycohydrolase from Rhodospirillum rubrum

Antharavally, Babu S., Poyner, Russell R., Zhang, Yaoping, Roberts, Gary P., Ludden, Paul W.

Site-directed mutagenesis of the draG gene was used to generate altered forms of dinitrogenase reductase-activating glycohydrolase (DRAG) with D123A, H142L, H158N, D243G, and E279R substitutions. The...

Functional Characterization of Three GlnB Homologs in the Photosynthetic Bacterium Rhodospirillum rubrum: Roles in Sensing Ammonium and Energy Status

Zhang, Yaoping, Pohlmann, Edward L., Ludden, Paul W., Roberts, Gary P.

The GlnB (PII) protein, the product of glnB, has been characterized previously in the photosynthetic bacterium Rhodospirillum rubrum. Here we describe identification of two other PII homologs in this...

Identification of critical residues in GlnB for its activation of NifA activity in the photosynthetic bacterium Rhodospirillum rubrum

Zhang, Yaoping, Pohlmann, Edward L., Roberts, Gary P.

The PII regulatory protein family is unusually widely distributed, being found in all three domains of life. Three PII homologs called GlnB, GlnK, and GlnJ have been identified in the photosynthetic...

GlnD Is Essential for NifA Activation, NtrB/NtrC-Regulated Gene Expression, and Posttranslational Regulation of Nitrogenase Activity in the Photosynthetic, Nitrogen-Fixing Bacterium Rhodospirillum rubrum

Zhang, Yaoping, Pohlmann, Edward L., Roberts, Gary P.

GlnD is a bifunctional uridylyltransferase/uridylyl-removing enzyme and is thought to be the primary sensor of nitrogen status in the cell. It plays an important role in nitrogen assimilation and...

Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

Reslewic, Susan, Zhou, Shiguo, Place, Mike, Zhang, Yaoping, Briska, Adam, Goldstein, Steve, ...

Rhodospirillum rubrum is a phototrophic purple nonsulfur bacterium known for its unique and well-studied nitrogen fixation and carbon monoxide oxidation systems and as a source of hydrogen and...

Identification of Rhodospirillum rubrum GlnB Variants That Are Altered in Their Ability To Interact with Different Targets in Response to Nitrogen Status Signals

Zhu, Yu, Conrad, Mary C., Zhang, Yaoping, Roberts, Gary P.

In Rhodospirillum rubrum, NifA, the transcriptional activator for the nif genes, is posttranslationally activated only by the uridylylated form of GlnB, one of three PII homologs in the organism. We...

Specificity and Regulation of Interaction between the PII and AmtB1 Proteins in Rhodospirillum rubrum▿

Wolfe, David M., Zhang, Yaoping, Roberts, Gary P.

The nitrogen regulatory protein PII and the ammonia gas channel AmtB are both found in most prokaryotes. Interaction between these two proteins has been observed in several organisms and may regulate...