Yasunori Machida

Plant Cells Without Detectable Plastids are Generated in the crumpled leaf Mutant of Arabidopsis thaliana (2009)

Chen, Yuling, Asano, Tomoya, Fujiwara, Makoto T., Yoshida, Shigeo, Machida, Yasunori, Yoshioka, Yasushi

Plastids are maintained in cells by proliferating prior to cell division and being partitioned to each daughter cell during cell division. It is unclear, however, whether cells without plastids are...

{beta}C1, the pathogenicity factor of TYLCCNV, interacts with AS1 to alter leaf development and suppress selective jasmonic acid responses (2008)

Yang, Jun-Yi, Iwasaki, Mayumi, Machida, Chiyoko, Machida, Yasunori, Zhou, Xueping, Chua, Nam-Hai

Viruses induce pathogenic symptoms on plants but the molecular basis is poorly understood. Here, we show that transgenic Arabidopsis expressing the pathogenesis protein βC1 of Tomato yellow leaf...

Phosphorylation of NtMAP65-1 by a MAP kinase down-regulates its activity of microtubule bundling and stimulates progression of cytokinesis of tobacco cells (2006)

Sasabe, Michiko, Soyano, Takashi, Takahashi, Yuji, Sonobe, Seiji, Igarashi, Hisako, Itoh, Tomohiko J., ...

The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by...

Oncogene 6b from Agrobacterium tumefaciens Induces Abaxial Cell Division at Late Stages of Leaf Development and Modifies Vascular Development in Petioles (2006)

Terakura, Shinji, Kitakura, Saeko, Ishikawa, Masaki, Ueno, Yoshihisa, Fujita, Tomomichi, Machida, Chiyoko, ...

The 6b gene in the T-DNA region of the Ti plasmids of Agrobacterium tumefaciens and A. vitis is able to generate shooty calli in phytohormone-free culture of leaf sections of tobacco transformed with...

Oncogene 6b from Agrobacterium tumefaciens Induces Abaxial Cell Division at Late Stages of Leaf Development and Modifies Vascular Development in Petioles (2006)

Terakura, Shinji, Kitakura, Saeko, Ishikawa, Masaki, Ueno, Yoshihisa, Fujita, Tomomichi, Machida, Chiyoko, ...

The 6b gene in the T-DNA region of the Ti plasmids of Agrobacterium tumefaciens and A. vitis is able to generate shooty calli in phytohormone-free culture of leaf sections of tobacco transformed with...

A Mitogen-Activated Protein Kinase NtMPK4 Activated by SIPKK Is Required for Jasmonic Acid Signaling and Involved in Ozone Tolerance via Stomatal Movement in Tobacco (2005)

Gomi, Kenji, Ogawa, Daisuke, Katou, Shinpei, Kamada, Hiroshi, Nakajima, Nobuyoshi, Saji, Hikaru, ...

The mitogen-activated protein kinase (MAPK) cascade is involved in responses to biotic and abiotic stress in plants. In this study, we isolated a new MAPK, NtMPK4, which is a tobacco homolog of...

A Mitogen-activated Protein Kinase NtMPK4 Activated by SIPKK is Required for Jasmonic Acid Signaling and Involved in Ozone Tolerance via Stomatal Movement in Tobacco (2005)

Gomi, Kenji, Ogawa, Daisuke, Katou, Shinpei, Kamada, Hiroshi, Nakajima, Nobuyoshi, Saji, Hikaru, ...

The mitogen-activated protein kinase (MAPK) cascade is involved in responses to biotic and abiotic stress in plants. In this study, we isolated a new MAPK, NtMPK4, which is a tobacco homolog of...

A Mitogen-Activated Protein Kinase NtMPK4 Activated by SIPKK Is Required for Jasmonic Acid Signaling and Involved in Ozone Tolerance via Stomatal Movement in Tobacco (2005)

Gomi, Kenji, Ogawa, Daisuke, Katou, Shinpei, Kamada, Hiroshi, Nakajima, Nobuyoshi, Saji, Hikaru, ...

The mitogen-activated protein kinase (MAPK) cascade is involved in responses to biotic and abiotic stress in plants. In this study, we isolated a new MAPK, NtMPK4, which is a tobacco homolog of...

A MAP Kinase Cascade That Controls Plant Cytokinesis (2004)

Takahashi, Yuji, Soyano, Takashi, Sasabe, Michiko, Machida, Yasunori

Several components of mitogen-activated protein kinase (MAPK) cascades have been identified in higher plants and have been implicated in cellular responses to a wide variety of abiotic and biotic...

The ASYMMETRIC LEAVES2 Gene of Arabidopsis thaliana, Required for Formation of a Symmetric Flat Leaf Lamina, Encodes a Member of a Novel Family of Proteins Characterized by Cysteine Repeats and a Leucine Zipper (2002)

Iwakawa, Hidekazu, Ueno, Yoshihisa, Semiarti, Endang, Onouchi, Hitoshi, Kojima, Shoko, Tsukaya, Hirokazu, ...

The ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana is involved in the establishment of the leaf venation system, which includes the prominent midvein, as well as in the development of a...

ACR4, a Putative Receptor Kinase Gene of Arabidopsis thaliana, that is Expressed in the Outer Cell Layers of Embryos and Plants, is Involved in Proper Embryogenesis (2002)

Tanaka, Hirokazu, Watanabe, Masaru, Watanabe, Daisuke, Tanaka, Toshihiro, Machida, Chiyoko, Machida, Yasunori

The surfaces of higher plants are characterized by epidermis, which usually consists of a single layer of cells. The epidermis is derived from the outer cell layer of the embryo or protoderm, which...

The Expression Pattern of the Gene for NPK1 Protein Kinase Related to Mitogen-Activated Protein Kinase Kinase Kinase (MAPKKK) in a Tobacco Plant: Correlation with Cell Proliferation (1998)

Nakashima, Marina, Hirano, Keiko, Nakashima, Seiko, Banno, Hiroharu, Nishihama, Ryuichi, Machida, Yasunori

Mitogen-activated protein kinase (MAPK) cascades consist of members of three families of protein kinases: the MAPK family, the MAPK kinase family, and the MAPK kinase kinase (MAPKKK) family. Some of...

Transient Gene Expression in Plant Cells Mediated by Agrobacterium tumefaciens: Application for the Analysis of Virulence Loci (1996)

Yoshioka, Yasushi, Takahashi, Yoshito, Matsuoka, Ken, Nakamura, Kenzo, Koizumi, Jun, Kojima, Mineo, ...

A simple system is described for detection of the transfer of T-DNA from Agrobacterium cells to suspension-cultured tobacco BY-2 cells. A modified reporter gene for rβ-glucuronidase (GUS) that...

Plant Homologues of Components of MAPK (Mitogen-Activated Protein Kinase) Signal Pathways in Yeast and Animal Cells (1995)

Nishihama, Ryuichi, Banno, Hiroharu, Shibata, Wataru, Hirano, Keiko, Nakashima, Marina, Usami, Shoji, ...

As they respond to numerous extracellular and intracellular stimuli, plants develop various morphological features and the capacity for a large variety of physiological processes during their growth....

Organ-Specific and Auxin-Inducible Expression of Two Tobacco par A-Related Genes in Transgenic Plants (1994)

Niwa, Yasuo, Muranaka, Toshiya, Baba, Akiko, Machida, Yasunori

We have isolated four genomic DNA clones that contain the transcription initiation site of the parA gene(s) from a tobacco genomic library by hybridization with the 5′ segment of the parA cDNA...

Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells (1991)

Onouchi, Hitoshi, Yokoi, Kumi, Machida, Chiyoko, Matsuzaki, Hiroaki, Oshima, Yasuji, Matsuoka, Ken, ...

Recombinase encoded by the R gene of pSR1 of Zygosaccharomyces rouxii mediates reciprocal recombination between two specific recombination sites (RSs) to induce excision or inversion of the DNA...

Right-hand border regions of octopine T-DNA are recognized by RNA polymerase of Agrobacterium as well as by VirD1 and VirD2 proteins (1988)

Niwa, Yasuo, Yamamoto, Akiko, Machida, Chiyoko, Takebe, Itaru, Machida, Yasunori

The T-DNA of octopine Ti plasmid of Agrobacterium tumefaciens contains TL- and TR-DNA regions each bounded by 25 base-pair-repeats (designated A, B, C and D from left to right). Short DNA segments...

Characterization of nascent DNA fragments produced by excision of uracil residues in DNA (1981)

Machida, Yasunori, Okazaki, Tuneko, Miyake, Tetsuo, Ohtsuka, Eiko, Ikehara, Morio

Nascent short DNA chains could result from repair of incorporated uracil residues or be intermediates in discontinuous replication. We have characterized short DNA chains having...

Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases

Bhalerao, Rishikesh P., Salchert, Klaus, Bakó, László, Ökrész, László, Szabados, László, Muranaka, Toshiya, ...

Mutation of the PRL1 gene, encoding a regulatory WD protein, results in glucose hypersensitivity and derepression of glucose-regulated genes in Arabidopsis. The yeast SNF1 protein kinase, a key...

Characterization of the transposition pattern of the Ac element in Arabidopsis thaliana using endonuclease I-SceI

Machida, Chiyoko, Onouchi, Hitoshi, Koizumi, Jun, Hamada, Susumu, Semiarti, Endang, Torikai, Satomi, ...

We have investigated physical distances and directions of transposition of the maize transposable element Ac in Arabidopsis thaliana. We prepared a transferred DNA (T-DNA) construct that carried a...

G2/M-Phase–Specific Transcription during the Plant Cell Cycle Is Mediated by c-Myb–Like Transcription Factors

Ito, Masaki, Araki, Satoshi, Matsunaga, Sachihiro, Itoh, Takashi, Nishihama, Ryuichi, Machida, Yasunori, ...

Plant B-type cyclin genes are expressed specifically in late G2- and M-phases during the cell cycle. Their promoters contain a common cis-acting element, called the MSA (M-specific activator)...

The Protein Encoded by Oncogene 6b from Agrobacterium tumefaciens Interacts with a Nuclear Protein of Tobacco

Kitakura, Saeko, Fujita, Tomomichi, Ueno, Yoshihisa, Terakura, Shinji, Wabiko, Hiroetsu, Machida, Yasunori

The 6b gene in the T-DNA from Agrobacterium has oncogenic activity in plant cells, inducing tumor formation, the phytohormone-independent division of cells, and alterations in leaf morphology. The...

NQK1/NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis

Soyano, Takashi, Nishihama, Ryuichi, Morikiyo, Kayoko, Ishikawa, Masaki, Machida, Yasunori

The tobacco protein kinase NPK1 is a MAPKKK that regulates formation of the cell plate during cytokinesis. In the present study, we have identified tobacco NQK1/NtMEK1 and NRK1 as a MAPKK and a MAPK,...

Factor inducing Agrobacterium tumefaciens vir gene expression is present in monocotyledonous plants

Usami, Shoji, Okamoto, Shigehisa, Takebe, Itaru, Machida, Yasunori

Agrobacterium tumefaciens harboring the tumor-inducing Ti plasmid incites crown gall tumor on dicotyledonous species. Upon infection of these plants, Ti plasmid DNA sequence is stably transferred...

Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1

Machida, Yasunori, Machida, Chiyoko, Ohtsubo, Hisako, Ohtsubo, Eiichi

We demonstrate that mutants with deletions at either end of the insertion sequence IS1 lose the ability to mediate cointegration of two plasmids, whereas mutants with deletions or an insertion within...

The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis

Nishihama, Ryuichi, Ishikawa, Masaki, Araki, Satoshi, Soyano, Takashi, Asada, Tetsuhiro, Machida, Yasunori

Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role...

Phosphorylation of NtMAP65-1 by a MAP kinase down-regulates its activity of microtubule bundling and stimulates progression of cytokinesis of tobacco cells

Sasabe, Michiko, Soyano, Takashi, Takahashi, Yuji, Sonobe, Seiji, Igarashi, Hisako, Itoh, Tomohiko J., ...

The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by...

Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases

Bhalerao, Rishikesh P., Salchert, Klaus, Bakó, László, Ökrész, László, Szabados, László, Muranaka, Toshiya, ...

Mutation of the PRL1 gene, encoding a regulatory WD protein, results in glucose hypersensitivity and derepression of glucose-regulated genes in Arabidopsis. The yeast SNF1 protein kinase, a key...

Characterization of the transposition pattern of the Ac element in Arabidopsis thaliana using endonuclease I-SceI

Machida, Chiyoko, Onouchi, Hitoshi, Koizumi, Jun, Hamada, Susumu, Semiarti, Endang, Torikai, Satomi, ...

We have investigated physical distances and directions of transposition of the maize transposable element Ac in Arabidopsis thaliana. We prepared a transferred DNA (T-DNA) construct that carried a...

G2/M-Phase–Specific Transcription during the Plant Cell Cycle Is Mediated by c-Myb–Like Transcription Factors

Ito, Masaki, Araki, Satoshi, Matsunaga, Sachihiro, Itoh, Takashi, Nishihama, Ryuichi, Machida, Yasunori, ...

Plant B-type cyclin genes are expressed specifically in late G2- and M-phases during the cell cycle. Their promoters contain a common cis-acting element, called the MSA (M-specific activator)...

The Protein Encoded by Oncogene 6b from Agrobacterium tumefaciens Interacts with a Nuclear Protein of Tobacco

Kitakura, Saeko, Fujita, Tomomichi, Ueno, Yoshihisa, Terakura, Shinji, Wabiko, Hiroetsu, Machida, Yasunori

The 6b gene in the T-DNA from Agrobacterium has oncogenic activity in plant cells, inducing tumor formation, the phytohormone-independent division of cells, and alterations in leaf morphology. The...

NQK1/NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis

Soyano, Takashi, Nishihama, Ryuichi, Morikiyo, Kayoko, Ishikawa, Masaki, Machida, Yasunori

The tobacco protein kinase NPK1 is a MAPKKK that regulates formation of the cell plate during cytokinesis. In the present study, we have identified tobacco NQK1/NtMEK1 and NRK1 as a MAPKK and a MAPK,...

Factor inducing Agrobacterium tumefaciens vir gene expression is present in monocotyledonous plants

Usami, Shoji, Okamoto, Shigehisa, Takebe, Itaru, Machida, Yasunori

Agrobacterium tumefaciens harboring the tumor-inducing Ti plasmid incites crown gall tumor on dicotyledonous species. Upon infection of these plants, Ti plasmid DNA sequence is stably transferred...

The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis

Nishihama, Ryuichi, Ishikawa, Masaki, Araki, Satoshi, Soyano, Takashi, Asada, Tetsuhiro, Machida, Yasunori

Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role...

Factors determining frequency of plasmid cointegration mediated by insertion sequence IS1

Machida, Yasunori, Machida, Chiyoko, Ohtsubo, Hisako, Ohtsubo, Eiichi

We demonstrate that mutants with deletions at either end of the insertion sequence IS1 lose the ability to mediate cointegration of two plasmids, whereas mutants with deletions or an insertion within...

Phosphorylation of NtMAP65-1 by a MAP kinase down-regulates its activity of microtubule bundling and stimulates progression of cytokinesis of tobacco cells

Sasabe, Michiko, Soyano, Takashi, Takahashi, Yuji, Sonobe, Seiji, Igarashi, Hisako, Itoh, Tomohiko J., ...

The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by...

Control of plant cytokinesis by an NPK1-mediated mitogen-activated protein kinase cascade.

Soyano, Takashi, Ishikawa, Masaki, Nishihama, Ryuichi, Araki, Satoshi, Ito, Mayumi, Ito, Masaki, ...

Cytokinesis is the last essential step in the distribution of genetic information to daughter cells and partition of the cytoplasm. In plant cells, various proteins have been found in the...

Histone Deacetylases and ASYMMETRIC LEAVES2 Are Involved in the Establishment of Polarity in Leaves of Arabidopsis[W]

Ueno, Yoshihisa, Ishikawa, Takaaki, Watanabe, Keiro, Terakura, Shinji, Iwakawa, Hidekazu, Okada, Kiyotaka, ...

We show that two Arabidopsis thaliana genes for histone deacetylases (HDACs), HDT1/HD2A and HDT2/HD2B, are required to establish leaf polarity in the presence of mutant ASYMMETRIC LEAVES2 (AS2) or...

An Oncoprotein from the Plant Pathogen Agrobacterium Has Histone Chaperone–Like Activity[W]

Terakura, Shinji, Ueno, Yoshihisa, Tagami, Hideaki, Kitakura, Saeko, Machida, Chiyoko, Wabiko, Hiroetsu, ...

Protein 6b, encoded by T-DNA from the pathogen Agrobacterium tumefaciens, stimulates the plant hormone–independent division of cells in culture in vitro and induces aberrant cell growth and the...

βC1, the pathogenicity factor of TYLCCNV, interacts with AS1 to alter leaf development and suppress selective jasmonic acid responses

Yang, Jun-Yi, Iwasaki, Mayumi, Machida, Chiyoko, Machida, Yasunori, Zhou, Xueping, Chua, Nam-Hai

Viruses induce pathogenic symptoms on plants but the molecular basis is poorly understood. Here, we show that transgenic Arabidopsis expressing the pathogenesis protein βC1 of Tomato yellow leaf...

Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana, and functional and molecular comparisons between AS2 and other family members

Matsumura, Yoko, Iwakawa, Hidekazu, Machida, Yasunori, Machida, Chiyoko

The ASYMMETRIC LEAVES2 (AS2) gene is required for the generation of the flat and symmetrical shape of the leaf lamina in Arabidopsis. AS2 encodes a plant-specific protein with an AS2/LATERAL ORGAN...