Yasushi Kawaguchi

Clinical usefulness of anti-RNA polymerase III antibody measurement by enzyme-linked immunosorbent assay (2009)

Satoh, Takashi, Ishikawa, Osamu, Ihn, Hironobu, Endo, Hirahito, Kawaguchi, Yasushi, Sasaki, Tetsuo, ...

Objective. To evaluate the clinical usefulness of measuring anti-RNA polymerase (RNAP) III antibody with a commercially available ELISA in Japanese patients with SSc. Methods. This multicentre study...

The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2 (2008)

Tanaka, Michiko, Sata, Tetsutaro, Kawaguchi, Yasushi

Abstract The herpes simplex virus 1 (HSV-1) UL7 gene is highly conserved among herpesviridae . Since the construction of recombinant HSV-1 with a mutation in the UL7 gene has not been reported, the...

Association of the diplotype configuration at the N-acetyltransferase 2 gene with adverse events with co-trimoxazole in Japanese patients with systemic lupus erythematosus (2007)

Soejima, Makoto, Sugiura, Tomoko, Kawaguchi, Yasushi, Kawamoto, Manabu, Katsumata, Yasuhiro, Takagi, Kae, ...

Abstract Although co-trimoxazole (trimethoprim-sulphamethoxazole) is an effective drug for prophylaxis against and treatment of Pneumocystis pneumonia, patients often experience adverse events with...

NOS2polymorphisms associated with the susceptibility to pulmonary arterial hypertension with systemic sclerosis: contribution to the transcriptional activity (2006)

Kawaguchi, Yasushi, Tochimoto, Akiko, Hara, Masako, Kawamoto, Manabu, Sugiura, Tomoko, Katsumata, Yasuhiro, ...

Abstract Systemic sclerosis (SSc) is a connective tissue disease characterized by tissue fibrosis. One of several complications of SSc, pulmonary arterial hypertension (PAH) can be refractory to...

Expression and function of inducible co-stimulator in patients with systemic lupus erythematosus: possible involvement in excessive interferon-γ and anti-double-stranded DNA antibody production (2006)

Kawamoto, Manabu, Harigai, Masayoshi, Hara, Masako, Kawaguchi, Yasushi, Tezuka, Katsunari, Tanaka, Michi, ...

Abstract Inducible co-stimulator (ICOS) is the third member of the CD28/cytotoxic T-lymphocyte associated antigen-4 family and is involved in the proliferation and activation of T cells. A detailed...

A promoter haplotype of the interleukin-18gene is associated with juvenile idiopathic arthritis in the Japanese population (2006)

Sugiura, Tomoko, Maeno, Nobuaki, Kawaguchi, Yasushi, Takei, Syuji, Imanaka, Hiroyuki, Kawano, Yoshifumi, ...

Abstract Recently, we reported that genetic polymorphisms within the human IL18 gene were associated with disease susceptibility to adult-onset Still's disease (AOSD), which is characterized by...

Increased IL-15 production of muscle cells in polymyositis and dermatomyositis (2002)

Sugiura, Tomoko, Harigai, Masayoshi, Kawaguchi, Yasushi, Takagi, Kae, Fukasawa, Chikako, Ohsako-Higami, Satomi, ...

In polymyositis (PM)/dermatomyositis (DM), various cytokines, especially macrophage‐derived cytokines such as IL‐1α, IL‐1β and tumor necrosis factor (TNF)‐α, are expressed...

A single amino acid substitution in the cyclin D binding domain of the infected cell protein no. 0 abrogates the neuroinvasiveness of herpes simplex virus without affecting its ability to replicate

Van Sant, Charles, Kawaguchi, Yasushi, Roizman, Bernard

The infected cell protein no. 0 (ICP0) of herpes simplex virus 1 is a promiscuous transactivator shown to enhance the expression of genes introduced into cells by infection or transfection. The...

Herpes simplex virus 1 α regulatory protein ICP0 functionally interacts with cellular transcription factor BMAL1

Kawaguchi, Yasushi, Tanaka, Michiko, Yokoymama, Akihiko, Matsuda, Go, Kato, Kentaro, Kagawa, Hiroyuki, ...

The infected cell protein no. 0 (ICP0) of herpes simplex virus 1 (HSV-1) is a promiscuous transactivator shown to enhance the expression of gene introduced into cells by infection or transfection. At...

Interaction of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP) with HS1-Associated Protein X-1: Implication of Cytoplasmic Function of EBNA-LP

Kawaguchi, Yasushi, Nakajima, Kaori, Igarashi, Mie, Morita, Tomoko, Tanaka, Michiko, Suzuki, Mikiko, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) consists of W1W2 repeats and a unique C-terminal Y1Y2 domain and has been suggested to play an important role in EBV-induced...

Cellular Elongation Factor 1δ Is Modified in Cells Infected with Representative Alpha-, Beta-, or Gammaherpesviruses

Kawaguchi, Yasushi, Matsumura, Tomio, Roizman, Bernard, Hirai, Kanji

Earlier reports (Y. Kawaguchi, R. Bruni, and B. Roizman, J. Virol. 71:1019–1024, 1997; Y. Kawaguchi, C. Van Sant, and B. Roizman, J. Virol. 72:1731–1736, 1998) showed that herpes simplex virus 1...

Eukaryotic Elongation Factor 1δ Is Hyperphosphorylated by the Protein Kinase Encoded by the UL13 Gene of Herpes Simplex Virus 1

Kawaguchi, Yasushi, Van Sant, Charles, Roizman, Bernard

The translation elongation factor 1δ (EF-1δ) consists of two forms, a hypophosphorylated form (apparent Mr, 38,000) and a hyperphosphorylated form (apparent Mr, 40,000). Earlier Y. Kawaguchi, R....

Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

Sonoda, Kengo, Sakaguchi, Masashi, Okamura, Hiroshi, Yokogawa, Kenji, Tokunaga, Eiji, Tokiyoshi, Sachio, ...

An earlier report (M. Sakaguchi et al., Vaccine 16:472–479, 1998) showed that recombinant Marek's disease virus type 1 (rMDV1) expressing the fusion (F) protein of Newcastle disease virus (NDV-F)...

Identification of Major Phosphorylation Sites of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP): Ability of EBNA-LP To Induce Latent Membrane Protein 1 Cooperatively with EBNA-2 Is Regulated by Phosphorylation†

Yokoyama, Akihiko, Tanaka, Michiko, Matsuda, Go, Kato, Kentaro, Kanamori, Mikiko, Kawasaki, Hiroshi, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) is a phosphoprotein suggested to play important roles in EBV-induced immortalization of B cells. One of the potential functions of...

Conserved Region CR2 of Epstein-Barr Virus Nuclear Antigen Leader Protein Is a Multifunctional Domain That Mediates Self-Association as well as Nuclear Localization and Nuclear Matrix Association

Tanaka, Michiko, Yokoyama, Akihiko, Igarashi, Mie, Matsuda, Go, Kato, Kentaro, Kanamori, Mikiko, ...

Self-association of viral proteins is important for many of their functions, including enzymatic, transcriptional, and transformational activities. Epstein-Barr virus (EBV) nuclear antigen leader...

Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo

Tanaka, Michiko, Kagawa, Hiroyuki, Yamanashi, Yuji, Sata, Tetsutaro, Kawaguchi, Yasushi

In recent years, several laboratories have reported on the cloning of herpes simplex virus type 1 (HSV-1) genomes as bacterial artificial chromosomes (BACs) in Escherichia coli and on procedures to...

Conserved Protein Kinases Encoded by Herpesviruses and Cellular Protein Kinase cdc2 Target the Same Phosphorylation Site in Eukaryotic Elongation Factor 1δ

Kawaguchi, Yasushi, Kato, Kentaro, Tanaka, Michiko, Kanamori, Mikiko, Nishiyama, Yukihiro, Yamanashi, Yuji

Earlier studies have shown that translation elongation factor 1δ (EF-1δ) is hyperphosphorylated in various mammalian cells infected with representative alpha-, beta-, and gammaherpesviruses and...

Nuclear Magnetic Resonance Studies of Poly(3-Hydroxybutyrate) and Polyphosphate Metabolism in Alcaligenes eutrophus

Doi, Yoshiharu, Kawaguchi, Yasushi, Nakamura, Yoshiyuki, Kunioka, Masao

The metabolic pathways of poly(3-hydroxybutyrate) (PHB) and polyphosphate in the microorganism Alcaligenes eutrophus H16 were studied by 1H, 13C, and 31P nuclear magnetic resonance (NMR) spectroscopy...

Epstein-Barr Virus Nuclear Antigen Leader Protein Induces Expression of Thymus- and Activation-Regulated Chemokine in B Cells

Kanamori, Mikiko, Watanabe, Shinya, Honma, Reiko, Kuroda, Masayuki, Imai, Shosuke, Takada, Kenzo, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) plays a critical role in transformation of primary B lymphocytes to continuously proliferating lymphoblastoid cell lines (LCLs). To...

Endogenous IL-1α from systemic sclerosis fibroblasts induces IL-6 and PDGF-A

Kawaguchi, Yasushi, Hara, Masako, Wright, Timothy M.

It is reported that fibroblasts derived from clinically affected skin areas of patients with systemic sclerosis (SSc) have the ability to overproduce several cytokines and growth factors (i.e., IL-6,...

Herpes Simplex Virus Type 1 UL51 Protein Is Involved in Maturation and Egress of Virus Particles

Nozawa, Naoki, Kawaguchi, Yasushi, Tanaka, Michiko, Kato, Akihisa, Kato, Ai, Kimura, Hiroshi, ...

The UL51 gene of herpes simplex virus type 1 (HSV-1) encodes a phosphoprotein whose homologs are conserved throughout the herpes virus family. Recently, we reported that UL51 protein colocalizes with...

Identification of Proteins Phosphorylated Directly by the Us3 Protein Kinase Encoded by Herpes Simplex Virus 1

Kato, Akihisa, Yamamoto, Mayuko, Ohno, Takashi, Kodaira, Hiroshi, Nishiyama, Yukihiro, Kawaguchi, Yasushi

We have developed a system to analyze the specific protein kinase activity of herpes simplex virus 1 Us3 in vitro and shown that Us3 directly phosphorylates viral proteins UL34, ICP22, and Us9 and...

Herpes Simplex Virus 1-Encoded Protein Kinase UL13 Phosphorylates Viral Us3 Protein Kinase and Regulates Nuclear Localization of Viral Envelopment Factors UL34 and UL31

Kato, Akihisa, Yamamoto, Mayuko, Ohno, Takashi, Tanaka, Michiko, Sata, Tetsutaro, Nishiyama, Yukihiro, ...

UL13 and Us3 are protein kinases encoded by herpes simplex virus 1. We report here that Us3 is a physiological substrate for UL13 in infected cells, based on the following observations. (i) The...

A promoter haplotype of the interleukin-18 gene is associated with juvenile idiopathic arthritis in the Japanese population

Sugiura, Tomoko, Maeno, Nobuaki, Kawaguchi, Yasushi, Takei, Syuji, Imanaka, Hiroyuki, Kawano, Yoshifumi, ...

Recently, we reported that genetic polymorphisms within the human IL18 gene were associated with disease susceptibility to adult-onset Still's disease (AOSD), which is characterized by...

Expression and function of inducible co-stimulator in patients with systemic lupus erythematosus: possible involvement in excessive interferon-γ and anti-double-stranded DNA antibody production

Kawamoto, Manabu, Harigai, Masayoshi, Hara, Masako, Kawaguchi, Yasushi, Tezuka, Katsunari, Tanaka, Michi, ...

Inducible co-stimulator (ICOS) is the third member of the CD28/cytotoxic T-lymphocyte associated antigen-4 family and is involved in the proliferation and activation of T cells. A detailed functional...

Epstein-Barr Virus Protein Kinase BGLF4 Is a Virion Tegument Protein That Dissociates from Virions in a Phosphorylation-Dependent Process and Phosphorylates the Viral Immediate-Early Protein BZLF1

Asai, Risa, Kato, Ai, Kato, Kentaro, Kanamori-Koyama, Mikiko, Sugimoto, Ken, Sairenji, Takeshi, ...

Epstein-Barr virus (EBV) BGLF4 is a viral protein kinase that is expressed in the lytic phase of infection and is packaged in virions. We report here that BGLF4 is a tegument protein that dissociates...

A single amino acid substitution in the cyclin D binding domain of the infected cell protein no. 0 abrogates the neuroinvasiveness of herpes simplex virus without affecting its ability to replicate

Van Sant, Charles, Kawaguchi, Yasushi, Roizman, Bernard

The infected cell protein no. 0 (ICP0) of herpes simplex virus 1 is a promiscuous transactivator shown to enhance the expression of genes introduced into cells by infection or transfection. The...

Herpes simplex virus 1 α regulatory protein ICP0 functionally interacts with cellular transcription factor BMAL1

Kawaguchi, Yasushi, Tanaka, Michiko, Yokoymama, Akihiko, Matsuda, Go, Kato, Kentaro, Kagawa, Hiroyuki, ...

The infected cell protein no. 0 (ICP0) of herpes simplex virus 1 (HSV-1) is a promiscuous transactivator shown to enhance the expression of gene introduced into cells by infection or transfection. At...

Interaction of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP) with HS1-Associated Protein X-1: Implication of Cytoplasmic Function of EBNA-LP

Kawaguchi, Yasushi, Nakajima, Kaori, Igarashi, Mie, Morita, Tomoko, Tanaka, Michiko, Suzuki, Mikiko, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) consists of W1W2 repeats and a unique C-terminal Y1Y2 domain and has been suggested to play an important role in EBV-induced...

Cellular Elongation Factor 1δ Is Modified in Cells Infected with Representative Alpha-, Beta-, or Gammaherpesviruses

Kawaguchi, Yasushi, Matsumura, Tomio, Roizman, Bernard, Hirai, Kanji

Earlier reports (Y. Kawaguchi, R. Bruni, and B. Roizman, J. Virol. 71:1019–1024, 1997; Y. Kawaguchi, C. Van Sant, and B. Roizman, J. Virol. 72:1731–1736, 1998) showed that herpes simplex virus 1...

Eukaryotic Elongation Factor 1δ Is Hyperphosphorylated by the Protein Kinase Encoded by the UL13 Gene of Herpes Simplex Virus 1

Kawaguchi, Yasushi, Van Sant, Charles, Roizman, Bernard

The translation elongation factor 1δ (EF-1δ) consists of two forms, a hypophosphorylated form (apparent Mr, 38,000) and a hyperphosphorylated form (apparent Mr, 40,000). Earlier Y. Kawaguchi, R....

Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

Sonoda, Kengo, Sakaguchi, Masashi, Okamura, Hiroshi, Yokogawa, Kenji, Tokunaga, Eiji, Tokiyoshi, Sachio, ...

An earlier report (M. Sakaguchi et al., Vaccine 16:472–479, 1998) showed that recombinant Marek's disease virus type 1 (rMDV1) expressing the fusion (F) protein of Newcastle disease virus (NDV-F)...

Identification of Major Phosphorylation Sites of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP): Ability of EBNA-LP To Induce Latent Membrane Protein 1 Cooperatively with EBNA-2 Is Regulated by Phosphorylation†

Yokoyama, Akihiko, Tanaka, Michiko, Matsuda, Go, Kato, Kentaro, Kanamori, Mikiko, Kawasaki, Hiroshi, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) is a phosphoprotein suggested to play important roles in EBV-induced immortalization of B cells. One of the potential functions of...

Conserved Region CR2 of Epstein-Barr Virus Nuclear Antigen Leader Protein Is a Multifunctional Domain That Mediates Self-Association as well as Nuclear Localization and Nuclear Matrix Association

Tanaka, Michiko, Yokoyama, Akihiko, Igarashi, Mie, Matsuda, Go, Kato, Kentaro, Kanamori, Mikiko, ...

Self-association of viral proteins is important for many of their functions, including enzymatic, transcriptional, and transformational activities. Epstein-Barr virus (EBV) nuclear antigen leader...

Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo

Tanaka, Michiko, Kagawa, Hiroyuki, Yamanashi, Yuji, Sata, Tetsutaro, Kawaguchi, Yasushi

In recent years, several laboratories have reported on the cloning of herpes simplex virus type 1 (HSV-1) genomes as bacterial artificial chromosomes (BACs) in Escherichia coli and on procedures to...

Conserved Protein Kinases Encoded by Herpesviruses and Cellular Protein Kinase cdc2 Target the Same Phosphorylation Site in Eukaryotic Elongation Factor 1δ

Kawaguchi, Yasushi, Kato, Kentaro, Tanaka, Michiko, Kanamori, Mikiko, Nishiyama, Yukihiro, Yamanashi, Yuji

Earlier studies have shown that translation elongation factor 1δ (EF-1δ) is hyperphosphorylated in various mammalian cells infected with representative alpha-, beta-, and gammaherpesviruses and...

Nuclear Magnetic Resonance Studies of Poly(3-Hydroxybutyrate) and Polyphosphate Metabolism in Alcaligenes eutrophus

Doi, Yoshiharu, Kawaguchi, Yasushi, Nakamura, Yoshiyuki, Kunioka, Masao

The metabolic pathways of poly(3-hydroxybutyrate) (PHB) and polyphosphate in the microorganism Alcaligenes eutrophus H16 were studied by 1H, 13C, and 31P nuclear magnetic resonance (NMR) spectroscopy...

Epstein-Barr Virus Nuclear Antigen Leader Protein Induces Expression of Thymus- and Activation-Regulated Chemokine in B Cells

Kanamori, Mikiko, Watanabe, Shinya, Honma, Reiko, Kuroda, Masayuki, Imai, Shosuke, Takada, Kenzo, ...

Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) plays a critical role in transformation of primary B lymphocytes to continuously proliferating lymphoblastoid cell lines (LCLs). To...

Endogenous IL-1α from systemic sclerosis fibroblasts induces IL-6 and PDGF-A

Kawaguchi, Yasushi, Hara, Masako, Wright, Timothy M.

It is reported that fibroblasts derived from clinically affected skin areas of patients with systemic sclerosis (SSc) have the ability to overproduce several cytokines and growth factors (i.e., IL-6,...

Herpes Simplex Virus Type 1 UL51 Protein Is Involved in Maturation and Egress of Virus Particles

Nozawa, Naoki, Kawaguchi, Yasushi, Tanaka, Michiko, Kato, Akihisa, Kato, Ai, Kimura, Hiroshi, ...

The UL51 gene of herpes simplex virus type 1 (HSV-1) encodes a phosphoprotein whose homologs are conserved throughout the herpes virus family. Recently, we reported that UL51 protein colocalizes with...

Identification of Proteins Phosphorylated Directly by the Us3 Protein Kinase Encoded by Herpes Simplex Virus 1

Kato, Akihisa, Yamamoto, Mayuko, Ohno, Takashi, Kodaira, Hiroshi, Nishiyama, Yukihiro, Kawaguchi, Yasushi

We have developed a system to analyze the specific protein kinase activity of herpes simplex virus 1 Us3 in vitro and shown that Us3 directly phosphorylates viral proteins UL34, ICP22, and Us9 and...

Herpes Simplex Virus 1-Encoded Protein Kinase UL13 Phosphorylates Viral Us3 Protein Kinase and Regulates Nuclear Localization of Viral Envelopment Factors UL34 and UL31

Kato, Akihisa, Yamamoto, Mayuko, Ohno, Takashi, Tanaka, Michiko, Sata, Tetsutaro, Nishiyama, Yukihiro, ...

UL13 and Us3 are protein kinases encoded by herpes simplex virus 1. We report here that Us3 is a physiological substrate for UL13 in infected cells, based on the following observations. (i) The...

Epstein-Barr Virus Protein Kinase BGLF4 Is a Virion Tegument Protein That Dissociates from Virions in a Phosphorylation-Dependent Process and Phosphorylates the Viral Immediate-Early Protein BZLF1

Asai, Risa, Kato, Ai, Kato, Kentaro, Kanamori-Koyama, Mikiko, Sugimoto, Ken, Sairenji, Takeshi, ...

Epstein-Barr virus (EBV) BGLF4 is a viral protein kinase that is expressed in the lytic phase of infection and is packaged in virions. We report here that BGLF4 is a tegument protein that dissociates...

A promoter haplotype of the interleukin-18 gene is associated with juvenile idiopathic arthritis in the Japanese population

Sugiura, Tomoko, Maeno, Nobuaki, Kawaguchi, Yasushi, Takei, Syuji, Imanaka, Hiroyuki, Kawano, Yoshifumi, ...

Recently, we reported that genetic polymorphisms within the human IL18 gene were associated with disease susceptibility to adult-onset Still's disease (AOSD), which is characterized by...

Expression and function of inducible co-stimulator in patients with systemic lupus erythematosus: possible involvement in excessive interferon-γ and anti-double-stranded DNA antibody production

Kawamoto, Manabu, Harigai, Masayoshi, Hara, Masako, Kawaguchi, Yasushi, Tezuka, Katsunari, Tanaka, Michi, ...

Inducible co-stimulator (ICOS) is the third member of the CD28/cytotoxic T-lymphocyte associated antigen-4 family and is involved in the proliferation and activation of T cells. A detailed functional...

NOS2 polymorphisms associated with the susceptibility to pulmonary arterial hypertension with systemic sclerosis: contribution to the transcriptional activity

Kawaguchi, Yasushi, Tochimoto, Akiko, Hara, Masako, Kawamoto, Manabu, Sugiura, Tomoko, Katsumata, Yasuhiro, ...

Systemic sclerosis (SSc) is a connective tissue disease characterized by tissue fibrosis. One of several complications of SSc, pulmonary arterial hypertension (PAH) can be refractory to treatment,...

Phosphorylation of MCM4 at Sites Inactivating DNA Helicase Activity of the MCM4-MCM6-MCM7 Complex during Epstein-Barr Virus Productive Replication

Kudoh, Ayumi, Daikoku, Tohru, Ishimi, Yukio, Kawaguchi, Yasushi, Shirata, Noriko, Iwahori, Satoko, ...

Induction of Epstein-Barr virus (EBV) lytic replication blocks chromosomal DNA replication notwithstanding an S-phase-like cellular environment with high cyclin-dependent kinase (CDK) activity. We...

Intracellular IL-1α-binding proteins contribute to biological functions of endogenous IL-1α in systemic sclerosis fibroblasts

Kawaguchi, Yasushi, Nishimagi, Emi, Tochimoto, Akiko, Kawamoto, Manabu, Katsumata, Yasuhiro, Soejima, Makoto, ...

The aberrant production of precursor IL-1α (pre-IL-1α) in skin fibroblasts that are derived from systemic sclerosis (SSc) is associated with the induction of IL-6 and procollagen, which contributes...

Association of the diplotype configuration at the N-acetyltransferase 2 gene with adverse events with co-trimoxazole in Japanese patients with systemic lupus erythematosus

Soejima, Makoto, Sugiura, Tomoko, Kawaguchi, Yasushi, Kawamoto, Manabu, Katsumata, Yasuhiro, Takagi, Kae, ...

Although co-trimoxazole (trimethoprim-sulphamethoxazole) is an effective drug for prophylaxis against and treatment of Pneumocystis pneumonia, patients often experience adverse events with this...

Truncated Form of the Epstein-Barr Virus Protein EBNA-LP Protects against Caspase-Dependent Apoptosis by Inhibiting Protein Phosphatase 2A▿

Garibal, Julie, Hollville, Émilie, Bell, Andrew I., Kelly, Gemma L., Renouf, Benjamin, Kawaguchi, Yasushi, ...

The Epstein-Barr virus (EBV)-encoded leader protein, EBNA-LP, strongly activates the EBNA2-mediated transcriptional activation of cellular and viral genes and is therefore important for EBV-induced...

Enhanced Phosphorylation of Transcription Factor Sp1 in Response to Herpes Simplex Virus Type 1 Infection Is Dependent on the Ataxia Telangiectasia-Mutated Protein▿

Iwahori, Satoko, Shirata, Noriko, Kawaguchi, Yasushi, Weller, Sandra K., Sato, Yoshitaka, Kudoh, Ayumi, ...

The ataxia telangiectasia-mutated (ATM) protein, a member of the related phosphatidylinositol 3-like kinase family encoded by a gene responsible for the human genetic disorder ataxia telangiectasia,...

Simultaneous Tracking of Capsid, Tegument, and Envelope Protein Localization in Living Cells Infected with Triply Fluorescent Herpes Simplex Virus 1▿

Sugimoto, Ken, Uema, Masashi, Sagara, Hiroshi, Tanaka, Michiko, Sata, Tetsutaro, Hashimoto, Yasuhiro, ...

We report here the construction of a triply fluorescent-tagged herpes simplex virus 1 (HSV-1) expressing capsid protein VP26, tegument protein VP22, and envelope protein gB as fusion proteins with...

Identification of a Physiological Phosphorylation Site of the Herpes Simplex Virus 1-Encoded Protein Kinase Us3 Which Regulates Its Optimal Catalytic Activity In Vitro and Influences Its Function in Infected Cells▿

Kato, Akihisa, Tanaka, Michiko, Yamamoto, Mayuko, Asai, Risa, Sata, Tetsutaro, Nishiyama, Yukihiro, ...

Us3 is a serine/threonine protein kinase encoded by herpes simplex virus 1 (HSV-1). Here, we report the identification of a physiological Us3 phosphorylation site on serine at position 147 (Ser-147)...

The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2

Tanaka, Michiko, Sata, Tetsutaro, Kawaguchi, Yasushi

The herpes simplex virus 1 (HSV-1) UL7 gene is highly conserved among herpesviridae. Since the construction of recombinant HSV-1 with a mutation in the UL7 gene has not been reported, the involvement...

Herpes Simplex Virus 1 Protein Kinase Us3 Phosphorylates Viral Envelope Glycoprotein B and Regulates Its Expression on the Cell Surface▿

Kato, Akihisa, Arii, Jun, Shiratori, Ikuo, Akashi, Hiroomi, Arase, Hisashi, Kawaguchi, Yasushi

Us3 is a serine-threonine protein kinase encoded by herpes simplex virus 1 (HSV-1). As reported here, we attempted to identify the previously unreported physiological substrate of Us3 in...

Herpesvirus gB-Induced Fusion between the Virion Envelope and Outer Nuclear Membrane during Virus Egress Is Regulated by the Viral US3 Kinase▿

Wisner, Todd W., Wright, Catherine C., Kato, Akihisa, Kawaguchi, Yasushi, Mou, Fan, Baines, Joel D., ...

Herpesvirus capsids collect along the inner surface of the nuclear envelope and bud into the perinuclear space. Enveloped virions then fuse with the outer nuclear membrane (NM). We previously showed...

Herpesvirus protein ICP27 switches PML isoform by altering mRNA splicing

Nojima, Takayuki, Oshiro-Ideue, Takako, Nakanoya, Hiroto, Kawamura, Hidenobu, Morimoto, Tomomi, Kawaguchi, Yasushi, ...

Viruses use alternative splicing to produce a broad series of proteins from small genomes by utilizing the cellular splicing machinery. Since viruses use cellular RNA binding proteins for viral RNA...

Entry of Herpes Simplex Virus 1 and Other Alphaherpesviruses via the Paired Immunoglobulin-Like Type 2 Receptor α▿

Arii, Jun, Uema, Masashi, Morimoto, Tomomi, Sagara, Hiroshi, Akashi, Hiroomi, Ono, Etsuro, ...

Herpes simplex virus 1 (HSV-1) enters cells either via fusion of the virion envelope and host cell plasma membrane or via endocytosis, depending on the cell type. In the study reported here, we...