Yasutada Imamura

Intercellular Accumulation of Type V Collagen Fibrils in Accordance with Cell Aggregation (2008)

Kihara, Takanori, Imamura, Yasutada, Takemura, Yukitoshi, Mizuno, Kazunori, Adachi, Eijiro, Hayashi, Toshihiko

We reported previously that human fibroblasts form clumps when cultured on a dish coated with reconstituted type V collagen fibrils. Essentially all the type V collagen fibrils, initially coated on...

Recognition of longer duplex DNA by cooperative strand invasion (2007)

Sugiyama, Toru, Imamura, Yasutada, Kurihara, Masaaki, Kittaka, Atsushi

Peptide nucleic acid is a synthetic DNA mimic in which the sugar-phosphate backbone has been replaced by a peptide backbone. A remarkable feature of PNA is its ability to recognize sequences within...

Sequence-specific recognition of double-stranded DNA by cooperative strand invasion (2006)

Sugiyama, Toru, Imamura, Yasutada, Hakamata, Wataru, Kurihara, Masaaki, Kittaka, Atsushi

A remarkable feature of peptide nucleic acid (PNA) is its ability to recognize some sequences within duplex DNA by strand invasion. In order to improve binding properties of PNA triplex invasion, we...

Cooperative strand invasion of double-stranded DNA by peptide nucleic acid (2005)

Sugiyama, Toru, Imamura, Yasutada, Hakamata, Wataru, Kurihara, Masaaki, Kittaka, Atsushi

Peptide nucleic acid is a synthetic DNA mimic in which the sugar-phosphate backbone has been replaced by a peptide backbone. A remarkable feature of PNA is its ability to recognize some sequences...

Secretion of Non-Helical Collagenous Pblypeptides of {alpha}1(IV) and {alpha}2(IV) Chains upon Depletion of Ascorbate by Cultured Human Cells (2001)

Yoshikawa, Kiwamu, Takahashi, Seiichiro, Imamura, Yasutada, Sado, Yoshikazu, Hayashi, Toshihiko

Our previous report showed that human fetal lung fibroblasts secreted non-disulfide-bonded, non-helical collagenous polypeptides of α1(IV) and α2(IV) chains depending on culture conditions...

Analysis of Matrix Protein Components of the Dermis-Like Structure Formed in a Long-Term Culture of Human Fibroblasts: Type VI Collagen Is a Major Component (1998)

Hazeki, Noriko, Yamato, Masayuki, Imamura, Yasutada, Sasaki, Tasuku, Nakazato, Koichi, Yamamoto, Keiichi, ...

Formation of a dermis-like structure by a long-term culture of fibroblasts in the presence of ascorbic acid is a potential model for tissue organization or wound healing, and has its practical use as...

The 160k {alpha}1(IV) Chain, a Short Form of a Type IV Collagen Polypeptide, of Bovine Lens Capsule Retains the NC1 Domain (1996)

Iwata, Masao, Sado, Yoshikazu, Sasaki, Tasuku, Imamura, Yasutada, Ninomiya, Yoshifumi, Hayashi, Toshihiko

We recently reported that the bovine lens capsule contained a shorter α1(IV) chain (160k) as a major polypeptide in addition to the 180k α1(IV) chain [J. Biochem. (1995) 117, 1298-1304]. Two...

Evidence for a Short Form of {alpha}1(IV) as a Major Polypeptide in Bovine Lens Capsule (1995)

Iwata, Masao, Imamura, Yasutada, Sasaki, Tasuku, Hayashi, Toshihiko

The extracts from bovine lens capsule with acetic acid contained, after reduction, three major collagenous polypeptides with Mr = 180k, 175k, and 160k, which were specifically immuno-stained...

Analysis of the Binding Sites of Gd3+ on Ca2+-Transporting ATPase of the Sarcoplasmic Reticulum through Its Effects on Fluorescence of Tryptophan Residues and a Covalently Attached Fluorescent Probe N-(l-Anilinonaphthyl-4)maleimide (1991)

Imamura, Yasutada, Kawakita, Masao

Interaction of Ca2+ and Gd3+ ions with Ca2+-transporting ATPase of the sarcoplasmic reticulum (SR-ATPase) was analyzed. Binding of Ca2+ to the transport site caused an enhancement...

Location of the Ca2+-Transport Site of Ca2+-Transporting ATPase of the Sarcoplasmic Reticulum as Determined by Analysis of Paramagnetic Interaction between Gd3+ Ions Bound at the Transport Site and Membrane-Embedded Nitroxide Spin Probes (1991)

Imamura, Yasutada, Kawakita, Masao

Gd3+ ions were bound to the Ca2+-transport site of Ca2+-transporting ATPase of the sarcoplasmic reticulum (SR-ATPase) and their effect on the ESR spectrum of spin-probes, which were...

Ca2+-Dependent Conformational Change of the ATP-Binding Site of Ca2+-Transporting ATPase of Sarcoplasmic Reticulum as Revealed by an Alteration of the Target-Site Specificity of Adenosine Tr iphosphopyridoxal (1989)

Yamamoto, Hisanori, Imamura, Yasutada, Tagaya, Mitsuo, Fukui, Toshio, Kawakita, Masao

Adenosinetriphosphopyridoxal (AP3PL) specifically modifies Lys684 of Ca2+-ATPase of sarcoplasmic reticulum (SR-ATPase) in the presence of Ca2+, leading to its inactivation (Yamamoto, H. et al. (1988)...

Purification of Limited Tryptic Fragments of Ca2+, Mg2+-Adenosine Triphosphatase of the Sarcoplasmic Reticulum and Identification of Conformation-Sensitive Cleavage Sites (1989)

Imamura, Yasutada, Kawakita, Masao

Sarcoplasmic reticulum membranes were treated with trypsin, and samples enriched with A1a, A1b, and C fragments (Saito, K. et al. (1984) J. Biochem. 95, 1297–1304), respectively, were prepared. A1b...

Limited Tryptic Digestion of Ca2+, Mg2+-Adenosine Triphosphatase of the Sarcoplasmic Reticulum: Enzymatic Properties of A1b+B Complex (1986)

IMAMURA, Yasutada, KAWAKITA, Masao

Sarcoplasmic reticulum membranes were treated with trypsin under conditions leading to accumulation of B and three other fragments a little smaller than A1 namely A1a, A1b, and C (Mr 27,000–28,000)...

Effect of Temperature and Added Ligands on the Susceptibility of Ca2+,Mg2+-Adenosine Triphosphatase of the Sarcoplasmic Reticulum to Trypsin (1984)

SAITO, Kimiko, IMAMURA, Yasutada, KAWAKITA, Masao

Sarcoplasmic reticulum membranes were subjected to limited proteolysis by trypsin under various conditions and the products were analyzed with SDS-polyacrylamide gel electrophoresis. Besides the...

Conformational Change of Ca2+,Mg2+-Adenosine Triphosphatase of Sarcoplasmic Reticulum upon Binding of Ca2+ and Adenyl-5'-yl-Imidodiphosphate as Detected by Trypsin Sensitivity Analysis (1984)

IMAMURA, Yasutada, SAITO, Kimiko, KAWAKITA, Masao

Ca2+-Mg2+-ATPase of sarcoplasmic reticulum was subjected to tryptic digestion under various conditions and the cleavage patterns were compared. The first tryptic cleavage to yield the...

Order of Intron Removal Influences Multiple Splice Outcomes, Including a Two-Exon Skip, in a COL5A1 Acceptor-Site Mutation That Results in Abnormal Pro-α1(V) N-Propeptides and Ehlers-Danlos Syndrome Type I

Takahara, Kazuhiko, Schwarze, Ulrike, Imamura, Yasutada, Hoffman, Guy G., Toriello, Helga, Smith, Lynne T., ...

Ehlers-Danlos syndrome (EDS) type I (the classical variety) is a dominantly inherited, genetically heterogeneous connective-tissue disorder. Mutations in the COL5A1 and COL5A2 genes, which encode...