Yi-Wei Tang

Plasminogen activator inhibitor-1 modulates adipocyte differentiation (2006)

Kanjanabuch, Talerngsak, Mao, Su-Li, Hao, Chuan-Ming, Tang, Yi-Wei, ...

Increased plasminogen activator inhibitor-1 (PAI-1) is linked to obesity and insulin resistance. However, the functional role of PAI-1 in adipocytes is unknown. This study was designed to investigate...

Comparison of Protein A Gene Sequencing with Pulsed-Field Gel Electrophoresis and Epidemiologic Data for Molecular Typing of Methicillin-Resistant Staphylococcus aureus

Tang, Yi-Wei, Waddington, Michael G., Smith, Douglas H., Manahan, Janice M., Kohner, Peggy C., Highsmith, Leanne M., ...

The epidemiologic relatedness of methicillin-resistant Staphylococcus aureus (MRSA) isolates is currently determined by analysis of chromosomal DNA restriction patterns by pulsed-field gel...

Identification of Coryneform Bacterial Isolates by Ribosomal DNA Sequence Analysis

Tang, Yi-Wei, Von Graevenitz, Alexander, Waddington, Michael G., Hopkins, Marlene K., Smith, Douglas H., Li, Haijing, ...

Identification of coryneform bacteria to the species level is important in certain circumstances for differentiating contamination and/or colonization from infection, which influences decisions...

Comparison of Human Immunodeficiency Virus Type 1 RNA Sequence Heterogeneity in Cerebrospinal Fluid and Plasma

Tang, Yi-Wei, Huong, Joe T.-J., Lloyd, Robert M., Spearman, Paul, Haas, David W.

The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma...

Direct Identification of Bacteria from Positive Blood Cultures by Amplification and Sequencing of the 16S rRNA Gene: Evaluation of BACTEC 9240 Instrument True- Positive and False-Positive Results

Qian, Qinfang, Tang, Yi-Wei, Kolbert, Christopher P., Torgerson, Catherine A., Hughes, John G., Vetter, Emily A., ...

In a previous study which evaluated the BACTEC 9240 automated blood culture system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.), we noted a 1.3% “instrument false-positive” rate....

Comparative Evaluation of Colorimetric Microtiter Plate Systems for Detection of Herpes Simplex Virus in Cerebrospinal Fluid

Tang, Yi-Wei, Rys, Paul N., Rutledge, Barbara J., Mitchell, P. Shawn, Smith, Thomas F., Persing, David H.

In the past few years, application of the PCR to the detection of herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) from patients with encephalitis and meningitis has become standard...

Comparison of Phenotypic and Genotypic Techniques for Identification of Unusual Aerobic Pathogenic Gram-Negative Bacilli

Tang, Yi-Wei, Ellis, Nicole M., Hopkins, Marlene K., Smith, Douglas H., Dodge, Deborah E., Persing, David H.

Rapid and accurate identification of bacterial pathogens is a fundamental goal of clinical microbiology, but one that is difficult or impossible for many slow-growing and fastidious organisms. We...

Interleukin-4 Diminishes CD8+ Respiratory Syncytial Virus-Specific Cytotoxic T-Lymphocyte Activity In Vivo

Aung, Sandra, Tang, Yi-Wei, Graham, Barney S.

Although interleukin-4 (IL-4) has been implicated in respiratory syncytial virus (RSV)-enhanced disease, the mechanism by which it modulates immune responses to primary RSV infection remains unclear....

Enterococcus faecium-Related Outbreak with Molecular Evidence of Transmission from Pigs to Humans

Lu, Hong-Zhou, Weng, Xin-Hua, Li, Haijing, Yin, You-Kuan, Pang, Mao-Yin, Tang, Yi-Wei

Between 24 July and 31 August 1998, thousands of domestic pigs died of hemorrhagic shock in three adjunct counties along the YangZi River in Jiangshu Province, China. From 28 July to 6 September...

Measurement of Human Cytomegalovirus Loads by Quantitative Real-Time PCR for Monitoring Clinical Intervention in Transplant Recipients

Li, Haijing, Dummer, J. Stephen, Estes, Wray R., Meng, Shufang, Wright, Peter F., Tang, Yi-Wei

Quantitative monitoring of human cytomegalovirus (HCMV) infection is helpful in determining appropriate antiviral management of transplant recipients. Quantitative PCR technologies have demonstrated...

Genetic Analysis of Crimean-Congo Hemorrhagic Fever Virus in Russia

Yashina, Lyudmila, Vyshemirskii, Oleg, Seregin, Sergei, Petrova, Irina, Samokhvalov, Evgeny, Lvov, Dmitry, ...

Genetic analysis of wild-type Crimean-Congo hemorrhagic fever (CCHF) virus strains recovered in the European part of Russia was performed. Reverse transcriptase PCR followed by direct sequencing was...

Rapidly Increasing Prevalence of Penicillin-Resistant Streptococcus pneumoniae in Middle Tennessee: a 10-Year Clinical and Molecular Analysis

Tang, Yi-Wei, Li, Haijing, Griffin, Jane P., Haas, David W., D'Agata, Erika M. C.

The clinical and molecular epidemiology of penicillin-resistant Streptococcus pneumoniae and the diagnostic accuracy of a six-primer PCR assay in identifying penicillin resistance were analyzed by...

Herpesvirus DNA Is Consistently Detected in Lungs of Patients with Idiopathic Pulmonary Fibrosis

Tang, Yi-Wei, Johnson, Joyce E., Browning, Philip J., Cruz-Gervis, Roberto A., Davis, Angela, Graham, Barney S., ...

On the basis of earlier reports associating Epstein-Barr Virus (EBV) with half of the cases of idiopathic pulmonary fibrosis (IPF), we hypothesized that chronic infection with EBV or a closely...

Major Outbreak of Toxic Shock-Like Syndrome Caused by Streptococcus mitis

Lu, Hong-Zhou, Weng, Xin-Hua, Zhu, Bai, Li, Haijing, Yin, You-Kuan, Zhang, Yong-Xin, ...

Severe illness caused by viridans streptococci rarely occurs in immunocompetent hosts. Between December 1990 and May 1991, thousands of patients in the YangZi River Delta area of Jiangsu Province,...

Characterization of a Novel Rapidly Growing Mycobacterium Species Associated with Sepsis

Hong, Tao, Ray Butler, W., Hollis, Frank, Floyd, Margaret M., Toney, Sean R., Tang, Yi-Wei, ...

A rapidly growing mycobacterium was isolated five times from blood cultures from a 6-year-old female patient with relapsed pre-B-cell acute lymphocytic leukemia. All five isolates had identical...

Surveillance of Childhood Influenza Virus Infection: What Is the Best Diagnostic Method To Use for Archival Samples?

Frisbie, Brent, Tang, Yi-Wei, Griffin, Marie, Poehling, Katherine, Wright, Peter F., Holland, Kathy, ...

Despite the clinical importance of influenza virus in pediatric respiratory infections, the optimal set of diagnostic tests to use when conducting studies using archival samples is not clear. In this...

Normal Values for CD4 and CD8 Lymphocyte Subsets in Healthy Chinese Adults from Shanghai

Jiang, Weiming, Kang, Laiyi, Lu, Hong-Zhou, Pan, Xiaozhang, Lin, Qingneng, Pan, Qichao, ...

The aim of this study was to establish reference ranges for lymphocyte subsets in Chinese adults. Venous blood specimens were obtained from 614 healthy, human immunodeficiency virus...

Clinical Use of 16S rRNA Gene Sequencing To Identify Mycoplasma felis and M. gateae Associated with Feline Ulcerative Keratitis

Gray, Larry D., Ketring, Kerry L., Tang, Yi-Wei

Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as...

Comparative Evaluation of Three Commercial Systems for Nucleic Acid Extraction from Urine Specimens

Tang, Yi-Wei, Sefers, Susan E., Li, Haijing, Kohn, Debra J., Procop, Gary W.

A nucleic acid extraction system that can handle small numbers of specimens with a short test turnaround time and short hands-on time is desirable for emergent testing. We performed a comparative...

Clonal Spread of Serogroup W135 Meningococcal Disease in Turkey

Kilic, Abdullah, Urwin, Rachel, Li, Haijing, Saracli, Mehmet A., Stratton, Charles W., Tang, Yi-Wei

Six cases of Neisseria meningitidis serogroup W135 meningococcal infection have been reported in Turkey since 2003. Seven isolates recovered from four meningococcal meningitis patients and two...

Hospital-Acquired Bordetella bronchiseptica Infection following Hematopoietic Stem Cell Transplantation

Huebner, Elizabeth S., Christman, Brian, Dummer, Stephen, Tang, Yi-Wei, Goodman, Stacey

Two patients who had undergone nonmyeloablative allogeneic stem cell transplantation 53 and 112 days earlier and were being monitored at the same transplant center developed severe Bordetella...

Comparison of Protein A Gene Sequencing with Pulsed-Field Gel Electrophoresis and Epidemiologic Data for Molecular Typing of Methicillin-Resistant Staphylococcus aureus

Tang, Yi-Wei, Waddington, Michael G., Smith, Douglas H., Manahan, Janice M., Kohner, Peggy C., Highsmith, Leanne M., ...

The epidemiologic relatedness of methicillin-resistant Staphylococcus aureus (MRSA) isolates is currently determined by analysis of chromosomal DNA restriction patterns by pulsed-field gel...

Identification of Coryneform Bacterial Isolates by Ribosomal DNA Sequence Analysis

Tang, Yi-Wei, Von Graevenitz, Alexander, Waddington, Michael G., Hopkins, Marlene K., Smith, Douglas H., Li, Haijing, ...

Identification of coryneform bacteria to the species level is important in certain circumstances for differentiating contamination and/or colonization from infection, which influences decisions...

Comparison of Human Immunodeficiency Virus Type 1 RNA Sequence Heterogeneity in Cerebrospinal Fluid and Plasma

Tang, Yi-Wei, Huong, Joe T.-J., Lloyd, Robert M., Spearman, Paul, Haas, David W.

The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma...

Direct Identification of Bacteria from Positive Blood Cultures by Amplification and Sequencing of the 16S rRNA Gene: Evaluation of BACTEC 9240 Instrument True- Positive and False-Positive Results

Qian, Qinfang, Tang, Yi-Wei, Kolbert, Christopher P., Torgerson, Catherine A., Hughes, John G., Vetter, Emily A., ...

In a previous study which evaluated the BACTEC 9240 automated blood culture system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.), we noted a 1.3% “instrument false-positive” rate....

Comparative Evaluation of Colorimetric Microtiter Plate Systems for Detection of Herpes Simplex Virus in Cerebrospinal Fluid

Tang, Yi-Wei, Rys, Paul N., Rutledge, Barbara J., Mitchell, P. Shawn, Smith, Thomas F., Persing, David H.

In the past few years, application of the PCR to the detection of herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) from patients with encephalitis and meningitis has become standard...

Comparison of Phenotypic and Genotypic Techniques for Identification of Unusual Aerobic Pathogenic Gram-Negative Bacilli

Tang, Yi-Wei, Ellis, Nicole M., Hopkins, Marlene K., Smith, Douglas H., Dodge, Deborah E., Persing, David H.

Rapid and accurate identification of bacterial pathogens is a fundamental goal of clinical microbiology, but one that is difficult or impossible for many slow-growing and fastidious organisms. We...

Interleukin-4 Diminishes CD8+ Respiratory Syncytial Virus-Specific Cytotoxic T-Lymphocyte Activity In Vivo

Aung, Sandra, Tang, Yi-Wei, Graham, Barney S.

Although interleukin-4 (IL-4) has been implicated in respiratory syncytial virus (RSV)-enhanced disease, the mechanism by which it modulates immune responses to primary RSV infection remains unclear....

Enterococcus faecium-Related Outbreak with Molecular Evidence of Transmission from Pigs to Humans

Lu, Hong-Zhou, Weng, Xin-Hua, Li, Haijing, Yin, You-Kuan, Pang, Mao-Yin, Tang, Yi-Wei

Between 24 July and 31 August 1998, thousands of domestic pigs died of hemorrhagic shock in three adjunct counties along the YangZi River in Jiangshu Province, China. From 28 July to 6 September...

Measurement of Human Cytomegalovirus Loads by Quantitative Real-Time PCR for Monitoring Clinical Intervention in Transplant Recipients

Li, Haijing, Dummer, J. Stephen, Estes, Wray R., Meng, Shufang, Wright, Peter F., Tang, Yi-Wei

Quantitative monitoring of human cytomegalovirus (HCMV) infection is helpful in determining appropriate antiviral management of transplant recipients. Quantitative PCR technologies have demonstrated...

Genetic Analysis of Crimean-Congo Hemorrhagic Fever Virus in Russia

Yashina, Lyudmila, Vyshemirskii, Oleg, Seregin, Sergei, Petrova, Irina, Samokhvalov, Evgeny, Lvov, Dmitry, ...

Genetic analysis of wild-type Crimean-Congo hemorrhagic fever (CCHF) virus strains recovered in the European part of Russia was performed. Reverse transcriptase PCR followed by direct sequencing was...

Rapidly Increasing Prevalence of Penicillin-Resistant Streptococcus pneumoniae in Middle Tennessee: a 10-Year Clinical and Molecular Analysis

Tang, Yi-Wei, Li, Haijing, Griffin, Jane P., Haas, David W., D'Agata, Erika M. C.

The clinical and molecular epidemiology of penicillin-resistant Streptococcus pneumoniae and the diagnostic accuracy of a six-primer PCR assay in identifying penicillin resistance were analyzed by...

Herpesvirus DNA Is Consistently Detected in Lungs of Patients with Idiopathic Pulmonary Fibrosis

Tang, Yi-Wei, Johnson, Joyce E., Browning, Philip J., Cruz-Gervis, Roberto A., Davis, Angela, Graham, Barney S., ...

On the basis of earlier reports associating Epstein-Barr Virus (EBV) with half of the cases of idiopathic pulmonary fibrosis (IPF), we hypothesized that chronic infection with EBV or a closely...

Major Outbreak of Toxic Shock-Like Syndrome Caused by Streptococcus mitis

Lu, Hong-Zhou, Weng, Xin-Hua, Zhu, Bai, Li, Haijing, Yin, You-Kuan, Zhang, Yong-Xin, ...

Severe illness caused by viridans streptococci rarely occurs in immunocompetent hosts. Between December 1990 and May 1991, thousands of patients in the YangZi River Delta area of Jiangsu Province,...

Characterization of a Novel Rapidly Growing Mycobacterium Species Associated with Sepsis

Hong, Tao, Ray Butler, W., Hollis, Frank, Floyd, Margaret M., Toney, Sean R., Tang, Yi-Wei, ...

A rapidly growing mycobacterium was isolated five times from blood cultures from a 6-year-old female patient with relapsed pre-B-cell acute lymphocytic leukemia. All five isolates had identical...

Surveillance of Childhood Influenza Virus Infection: What Is the Best Diagnostic Method To Use for Archival Samples?

Frisbie, Brent, Tang, Yi-Wei, Griffin, Marie, Poehling, Katherine, Wright, Peter F., Holland, Kathy, ...

Despite the clinical importance of influenza virus in pediatric respiratory infections, the optimal set of diagnostic tests to use when conducting studies using archival samples is not clear. In this...

Normal Values for CD4 and CD8 Lymphocyte Subsets in Healthy Chinese Adults from Shanghai

Jiang, Weiming, Kang, Laiyi, Lu, Hong-Zhou, Pan, Xiaozhang, Lin, Qingneng, Pan, Qichao, ...

The aim of this study was to establish reference ranges for lymphocyte subsets in Chinese adults. Venous blood specimens were obtained from 614 healthy, human immunodeficiency virus...

Clinical Use of 16S rRNA Gene Sequencing To Identify Mycoplasma felis and M. gateae Associated with Feline Ulcerative Keratitis

Gray, Larry D., Ketring, Kerry L., Tang, Yi-Wei

Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as...

Comparative Evaluation of Three Commercial Systems for Nucleic Acid Extraction from Urine Specimens

Tang, Yi-Wei, Sefers, Susan E., Li, Haijing, Kohn, Debra J., Procop, Gary W.

A nucleic acid extraction system that can handle small numbers of specimens with a short test turnaround time and short hands-on time is desirable for emergent testing. We performed a comparative...

Clonal Spread of Serogroup W135 Meningococcal Disease in Turkey

Kilic, Abdullah, Urwin, Rachel, Li, Haijing, Saracli, Mehmet A., Stratton, Charles W., Tang, Yi-Wei

Six cases of Neisseria meningitidis serogroup W135 meningococcal infection have been reported in Turkey since 2003. Seven isolates recovered from four meningococcal meningitis patients and two...

Hospital-Acquired Bordetella bronchiseptica Infection following Hematopoietic Stem Cell Transplantation

Huebner, Elizabeth S., Christman, Brian, Dummer, Stephen, Tang, Yi-Wei, Goodman, Stacey

Two patients who had undergone nonmyeloablative allogeneic stem cell transplantation 53 and 112 days earlier and were being monitored at the same transplant center developed severe Bordetella...

Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2

Allawi, Hatim T., Li, Haijing, Sander, Tamara, Aslanukov, Azamat, Lyamichev, Victor I., Blackman, Amondrea, ...

We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method...

Comparative Evaluation of Three Commercially Available Methodologies for Hepatitis C Virus Genotyping

Schutzbank, Ted E., Sefers, Susan E., Kahmann, Nicole, Li, Haijing, Tang, Yi-Wei

We compared the performances of three hepatitis C virus genotyping methodologies supplied by Bayer, Abbott, and Third Wave Technologies. Genotypes were determined for 136 of 137 specimens by the...

Simultaneous Amplification and Identification of 25 Human Papillomavirus Types with Templex Technology▿

Han, Jian, Swan, David C., Smith, Sharon J., Lum, Shanjuan H., Sefers, Susan E., Unger, Elizabeth R., ...

The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects...

Antimicrobial Susceptibility Patterns and Staphylococcal Cassette Chromosome mec Types of, as Well as Panton-Valentine Leukocidin Occurrence among, Methicillin-Resistant Staphylococcus aureus Isolates from Children and Adults in Middle Tennessee▿

Kilic, Abdullah, Li, Haijing, Stratton, Charles W., Tang, Yi-Wei

Antimicrobial susceptibility patterns, Panton-Valentine leukocidin (PVL) occurrence, and staphylococcal cassette chromosome mec (SCCmec) types in methicillin-resistant Staphylococcus aureus (MRSA)...

Necrotizing Fasciitis from Vibrio vulnificus in a Patient with Undiagnosed Hepatitis and Cirrhosis▿

Muldrew, Kenneth L., Miller, Russell R., Kressin, Megan, Tang, Yi-Wei, Stratton, Charles

Necrotizing fasciitis due to Vibrio vulnificus may result in overwhelming sepsis, leading to death in some patients. Significant risk factors for severe disease include preexisting liver disease. We...

Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection

Muldrew, Kenneth L., Simpson, Jean F., Stratton, Charles W., Tang, Yi-Wei

We report the use of molecular techniques in the diagnosis of a case of culture-negative necrotizing fasciitis occurring in a 32-year-old female with no significant past medical history and who died...

Detection of Medically Important Ehrlichia by Quantitative Multicolor TaqMan Real-Time Polymerase Chain Reaction of the dsb Gene

Doyle, C. Kuyler, Labruna, Marcelo B., Breitschwerdt, Edward B., Tang, Yi-Wei, Corstvet, Richard E., Hegarty, Barbara C., ...

Ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses, in addition to causing serious and fatal infections in companion animals and livestock. We...

StaphPlex System for Rapid and Simultaneous Identification of Antibiotic Resistance Determinants and Panton-Valentine Leukocidin Detection of Staphylococci from Positive Blood Cultures▿

Tang, Yi-Wei, Kilic, Abdullah, Yang, Qunying, McAllister, Sigrid K., Li, Haijing, Miller, Rebecca S., ...

Phenotypic methods take several days for identification and antimicrobial susceptibility testing of staphylococcal isolates after gram-positive cocci in clusters (GPCC) are observed in positive blood...

Simultaneous Detection and High-Throughput Identification of a Panel of RNA Viruses Causing Respiratory Tract Infections▿

Li, Haijing, McCormac, Melinda A., Estes, R. Wray, Sefers, Susan E., Dare, Ryan K., Chappell, James D., ...

Clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically...

Prevalence of and Molecular Basis for Tuberculosis Drug Resistance in the Republic of Georgia: Validation of a QIAplex System for Detection of Drug Resistance-Related Mutations▿

Gegia, Medea, Mdivani, Nino, Mendes, Rodrigo E., Li, Haijing, Akhalaia, Maka, Han, Jian, ...

We developed a QIAplex system for the simultaneous detection of 24 Mycobacterium tuberculosis gene mutations responsible for resistance to isoniazid (INH), rifampin (RIF), streptomycin (STM), and...

Early Infant Human Immunodeficiency Virus Type 1 Detection Suitable for Resource-Limited Settings with Multiple Circulating Subtypes by Use of Nested Three-Monoplex DNA PCR and Dried Blood Spots▿

Zhang, Qi, Wang, Linhong, Jiang, Yan, Fang, Liwen, Pan, Pinliang, Gong, Shuangyan, ...

The early detection of human immunodeficiency virus type 1 (HIV-1) infection in infants is complicated by the persistence of maternal antibodies and by diverse HIV-1 subtypes. We developed a nested,...

Acinetobacter septicus sp. nov. Association with a Nosocomial Outbreak of Bacteremia in a Neonatal Intensive Care Unit▿

Kilic, Abdullah, Li, Haijing, Mellmann, Alexander, Basustaoglu, Ahmet C., Kul, Mustafa, Senses, Zeynep, ...

Acinetobacter species other than Acinetobacter baumannii have rarely been reported to be associated with nosocomial outbreaks of bloodstream infections. Within a period of 1 week, seven...

Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Positive Blood Cultures by Isothermal Amplification and a Disposable Detection Device▿

Goldmeyer, James, Li, Haijing, McCormac, Melinda, Cook, Steve, Stratton, Charles, Lemieux, Bertrand, ...

A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing...

Prospective Evaluation of Rapid Antigen Tests for Diagnosis of Respiratory Syncytial Virus and Human Metapneumovirus Infections▿

Aslanzadeh, Jaber, Zheng, Xiaotian, Li, Haijing, Tetreault, Janice, Ratkiewicz, Irene, Meng, Shufang, ...

Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are two important viral pathogens that cause respiratory tract infections in the pediatric population. The rapid detection of these...

Multicenter Comparison of PCR Assays for Detection of Human Herpesvirus 6 DNA in Serum▿

Flamand, Louis, Gravel, Annie, Boutolleau, David, Alvarez-Lafuente, Roberto, Jacobson, Steve, Malnati, Mauro S., ...

Human herpesvirus 6 (HHV-6) is a ubiquitous virus with which infections have been associated with pathologies ranging from delayed bone marrow engraftment to a variety of neurological diseases. The...

MVPlex Assay for Direct Detection of Methicillin-Resistant Staphylococcus aureus in Naris and Other Swab Specimens▿

Podzorski, Raymond P., Li, Haijing, Han, Jian, Tang, Yi-Wei

We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant...

Clonal Dissemination of Staphylococcus epidermidis in an Oncology Ward▿

Muldrew, Kenneth L., Tang, Yi-Wei, Li, Haijing, Stratton, Charles W.

Coagulase-negative staphylococci (CoNS) are the main cause of catheter-related infections, especially among immunosuppressed and neutropenic patients, as well as a source of bacterial contamination...

Qualitative and Quantitative Detection of Chlamydophila pneumoniae DNA in Cerebrospinal Fluid from Multiple Sclerosis Patients and Controls

Tang, Yi-Wei, Sriram, Subramaniam, Li, Haijing, Yao, Song-yi, Meng, Shufang, Mitchell, William M., ...

A standardized molecular test for the detection of Chlamydophila pneumoniae DNA in cerebrospinal fluid (CSF) would assist the further assessment of the association of C. pneumoniae with multiple...

Application of Isothermal Helicase-Dependent Amplification with a Disposable Detection Device in a Simple Sensitive Stool Test for Toxigenic Clostridium difficile

Chow, Wing Huen A., McCloskey, Cindy, Tong, Yanhong, Hu, Lin, You, Qimin, Kelly, Ciarán P., ...

Enzyme immunoassays (EIAs) are commonly used for the diagnosis of cases of Clostridium difficile-associated diarrhea (CDAD). However, these EIAs have high false-negative rates, even in patients with...

Detection of Viruses in Human Adenoid Tissues by Use of Multiplex PCR▿

Sato, Masatoki, Li, Haijing, Ikizler, Mine R., Werkhaven, Jay A., Williams, John V., Chappell, James D., ...

By PCR, we detected a high frequency of viruses in adenoids obtained from children without acute respiratory symptoms. Our results suggest that persistent/latent viral infection in the respiratory...