Yoshifumi Takeda

Pre-Tango structures on curves (2002)

Takeda, Yoshifumi, Yokogawa, Kôji

The pre-Tango structure is a certain invertible sheaf of locally exact differentials on a curve in positive characteristic. On any curve of sufficiently high genus, there necessarily exist pre-Tango...

A nontoxic mutant of cholera toxin elicits Th2-type responses for enhanced mucosal immunity

Yamamoto, Shingo, Kiyono, Hiroshi, Yamamoto, Masafumi, Imaoka, Koichi, Yamamoto, Miho, Fujihashi, Kohtaro, ...

We have characterized a nontoxic mutant of cholera toxin (CT) as a mucosal adjuvant in mice. The mutant CT was made by substitution of serine with phenylalanine at position 61 of the A subunit...

A 1.2-Å snapshot of the final step of bacterial cell wall biosynthesis

Lee, Wenlin, McDonough, Michael A., Kotra, Lakshmi P., Li, Zhi-Hong, Silvaggi, Nicholas R., Takeda, Yoshifumi, ...

The cell wall imparts structural strength and shape to bacteria. It is made up of polymeric glycan chains with peptide branches that are cross-linked to form the cell wall. The cross-linking...

Clonal Diversity among Recently Emerged Strains of Vibrio parahaemolyticus O3:K6 Associated With Pandemic Spread

Bag, Prasanta K., Nandi, Suvobroto, Bhadra, Rupak K., Ramamurthy, T., Bhattacharya, S. K., Nishibuchi, M., ...

The genomes of the O3:K6 strains of Vibrio parahaemolyticus which abruptly emerged in Calcutta, India, in February 1996 and which demonstrated an unusual potential to spread and an enhanced...

Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India

Garg, Pallavi, Nandy, Ranjan K., Chaudhury, Papiya, Chowdhury, Nandini Roy, De, Keya, Ramamurthy, T., ...

The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular...

Virulence Genes in Environmental Strains of Vibrio cholerae

Chakraborty, Soumen, Mukhopadhyay, Asish K., Bhadra, Rupak Kumar, Ghosh, Amar Nath, Mitra, Rupak, Shimada, Toshio, ...

The virulence of a pathogen is dependent on a discrete set of genetic determinants and their well-regulated expression. The ctxAB and tcpA genes are known to play a cardinal role in maintaining...

Cell Vacuolation, a Manifestation of the El Tor Hemolysin of Vibrio cholerae

Mitra, Rupak, Figueroa, Paula, Mukhopadhyay, Asish K., Shimada, Toshio, Takeda, Yoshifumi, Berg, Douglas E., ...

Culture supernatants of nontoxigenic nonepidemic clinical strains of Vibrio cholerae belonging to diverse serogroups were found to induce vacuolation of nonconfluent HeLa cells. The vacuoles became...

Protective Effect of Lactobacillus casei Strain Shirota on Shiga Toxin-Producing Escherichia coli O157:H7 Infection in Infant Rabbits

Ogawa, Michinaga, Shimizu, Kensuke, Nomoto, Koji, Takahashi, Masatoshi, Watanuki, Masaaki, Tanaka, Ryuichiro, ...

We examined colonization patterns of Shiga toxin-producing Escherichia coli (STEC), concentrations of Shiga toxins (Stxs) and specific immunoglobulin A (lgA) against Stxs and STEC bacterial cell...

Characterization of VPI Pathogenicity Island and CTXφ Prophage in Environmental Strains of Vibrio cholerae

Mukhopadhyay, Asish K., Chakraborty, Soumen, Takeda, Yoshifumi, Nair, G. Balakrish, Berg, Douglas E.

Environmental isolates of Vibrio cholerae of eight randomly amplified polymorphic DNA (RAPD) fingerprint types from Calcutta, India, that were unusual in containing toxin-coregulated pilus or cholera...

Molecular Epidemiology of Reemergent Vibrio cholerae O139 Bengal in India

Mukhopadhyay, Asish Kumar, Basu, Arnab, Garg, Pallavi, Bag, Prasanta K., Ghosh, Amit, Bhattacharya, S. K., ...

We report the prevalence of the O139 serogroup in Calcutta, India, after its reemergence in August 1996 and the spread of the reemerged clone to other parts of the country by using previously...

Identification and Characterization of a Newly Isolated Shiga Toxin 2-Converting Phage from Shiga Toxin-Producing Escherichia coli

Watarai, Masahisa, Sato, Toshio, Kobayashi, Midori, Shimizu, Takeshi, Yamasaki, Shinji, Tobe, Toru, ...

Shiga toxins 1 (Stx1) and 2 (Stx2) are encoded by toxin-converting bacteriophages of Stx-producing Escherichia coli (STEC), and so far two Stx1- and one Stx2-converting phages have been isolated from...

Mutational Analysis of Superantigen Activity Responsible for the Induction of Skin Erythema by Streptococcal Pyrogenic Exotoxin C

Yamaoka, Junichi, Nakamura, Eijiro, Takeda, Yoshifumi, Imamura, Sadao, Minato, Nagahiro

Streptococcal pyrogenic exotoxin C (SPEC), when injected intradermally, induces erythema in unsensitized rabbits. In the present study, we examined whether this erythema induction is due to the...

Prevalence of the Pandemic Genotype of Vibrio parahaemolyticus in Dhaka, Bangladesh, and Significance of Its Distribution across Different Serotypes

Bhuiyan, N. A., Ansaruzzaman, M., Kamruzzaman, M., Alam, Khorshed, Chowdhury, N. R., Nishibuchi, M., ...

Sixty-six strains of Vibrio parahaemolyticus belonging to 14 serotypes were isolated from hospitalized patients in Dhaka, Bangladesh, from January 1998 to December 2000. Among these, 48 strains...

Escalating Association of Vibrio cholerae O139 with Cholera Outbreaks in India

Sinha, Sutapa, Chakraborty, Rupa, De, Keya, Khan, Asis, Datta, Simanti, Ramamurthy, T., ...

Between December 1999 and December 2000, teams from the National Institute of Cholera and Enteric Diseases, Calcutta, India, examined eight outbreaks of cholera, which occurred in different parts of...

Isolation of Shigella dysenteriae Type 1 and S. flexneri Strains from Surface Waters in Bangladesh: Comparative Molecular Analysis of Environmental Shigella Isolates versus Clinical Strains

Faruque, Shah M., Khan, Rasel, Kamruzzaman, M., Yamasaki, Shinji, Ahmad, Q. Shafi, Azim, Tasnim, ...

Bacillary dysentery caused by Shigella species is a public health problem in developing countries including Bangladesh. Although, shigellae-contaminated food and drinks are often the source of the...

Cloning and Characterization of Genes Encoding Homologues of the B Subunit of Cholera Toxin and the Escherichia coli Heat-Labile Enterotoxin from Clinical Isolates of Citrobacter freundii and E. coli

Karasawa, Tadahiro, Ito, Hideaki, Tsukamoto, Teizo, Yamasaki, Shinji, Kurazono, Hisao, Faruque, Shah M., ...

We identified and characterized a gene encoding a homologue of the B subunits of cholera toxin (CTB) and heat-labile enterotoxin (LTB) of Escherichia coli from a clinical isolate of Citrobacter...

Genome Analysis of a Novel Shiga Toxin 1 (Stx1)-Converting Phage Which Is Closely Related to Stx2-Converting Phages but Not to Other Stx1-Converting Phages

Sato, Toshio, Shimizu, Takeshi, Watarai, Masahisa, Kobayashi, Midori, Kano, Shigeyuki, Hamabata, Takashi, ...

Two Stx-converting phages, designated Stx1φ and Stx2φ-II, were isolated from an Escherichia coli O157:H7 strain, Morioka V526, and their entire nucleotide sequences were determined. The genomes of...

Antigenic Difference Between Polar Monotrichous and Peritrichous Flagella of Vibrio parahaemolyticus

Shinoda, Sumio, Honda, Takeshi, Takeda, Yoshifumi, Miwatani, Toshio

Polar monotrichous and peritrichous flagella of Vibrio parahaemolyticus were isolated and purified separately. On hydroxylapatite column chromatography, the flagellins of polar monotrichous flagella...

Characterization of Aminoacyl Transfer Ribonucleic Acid Formation Stimulated by Polyamines

Takeda, Yoshifumi, Matsuzaki, Kazuyoshi, Igarashi, Kazuei

From a comparative study of aminoacyl transfer ribonucleic acid (tRNA) formation stimulated by polyamines or by Mg2+, it is shown that both reactions have the same requirements for adenosine...

Association of Cytolethal Distending Toxin Locus cdtB with Enteropathogenic Escherichia coli Isolated from Patients with Acute Diarrhea in Calcutta, India

Pandey, Mritunjay, Khan, Asis, Das, Suresh C., Sarkar, Bhaswati, Kahali, Soumen, Chakraborty, Subhra, ...

Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These...

Emergence and evolution of Vibrio cholerae O139

Faruque, Shah M., Sack, David A., Sack, R. Bradley, Colwell, Rita R., Takeda, Yoshifumi, Nair, G. Balakrish

The emergence of Vibrio cholerae O139 Bengal during 1992–1993 was associated with large epidemics of cholera in India and Bangladesh and, initially, with a total displacement of the existing V....

Clonal Multidrug-Resistant Shigella dysenteriae Type 1 Strains Associated with Epidemic and Sporadic Dysenteries in Eastern India

Pazhani, Gururaja Perumal, Sarkar, Bhaswati, Ramamurthy, Thandavarayan, Bhattacharya, S. K., Takeda, Yoshifumi, Niyogi, S. K.

Multidrug-resistant strains of Shigella dysenteriae type 1 were implicated in three outbreaks and sporadic cases of dysentery in eastern India in 2002 and 2003. After a hiatus of 14 years, this...

Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7

Asahara, Takashi, Shimizu, Kensuke, Nomoto, Koji, Hamabata, Takashi, Ozawa, Ayako, Takeda, Yoshifumi

The anti-infectious activity of probiotic Bifidobacteria against Shiga toxin-producing Escherichia coli (STEC) O157:H7 was examined in a fatal mouse STEC infection model. Stable colonization of the...

Existence of Two Distinct Hemolysins in Vibrio parahaemolyticus

Sakurai, Jun, Matsuzaki, Akiko, Takeda, Yoshifumi, Miwatani, Toshio

Two distinct hemolysins were demonstrated in Vibrio parahaemolyticus. A thermostable direct hemolysin purified from V. parahemolyticus WP-1, a Kanagawa phenomenon (KP)-positive strain, is...

Demonstration of a Temperature-Dependent Inactivating Factor of the Thermostable Direct Hemolysin in Vibrio parahaemolyticus

Takeda, Yoshifumi, Hori, Yayoi, Miwatani, Toshio

A factor was found in Vibrio parahaemolyticus which inactivated the hemolytic activity of a purified, thermostable direct hemolysin. The inactivating factor was associated with the hemolysin but...

Effect of Heat (Arrhenius Effect) on Crude Hemolysin of Vibrio parahaemolyticus

Miwatani, Toshio, Takeda, Yoshifumi, Sakurai, Jun, Yoshihara, Akiko, Taga, Sekiko

Crude hemolysins prepared from various strains of Vibrio parahaemolyticus, which give positive Kanagawa phenomenon, were partly inactivated by heating at 60 C, but not inactivated significantly by...

Development of a PCR-Restriction Fragment Length Polymorphism Assay for the Epidemiological Analysis of Shiga Toxin-Producing Escherichia coli

Shima, Kensuke, Terajima, Jun, Sato, Toshio, Nishimura, Kazuhiko, Tamura, Kazumichi, Watanabe, Haruo, ...

Six characteristic regions (I to VI) were identified in Shiga toxin 2 (Stx2) phages (T. Sato, T. Shimizu, M. Watarai, M. Kobayashi, S. Kano, T. Hamabata, Y. Takeda, and S. Yamasaki, Gene 309:35-48,...

Diverse CTX Phages among Toxigenic Vibrio cholerae O1 and O139 Strains Isolated between 1994 and 2002 in an Area Where Cholera is Endemic in Bangladesh

Nusrin, Suraia, Khan, G. Yeahia, Bhuiyan, N. A., Ansaruzzaman, M., Hossain, M. A., Safa, Ashrafus, ...

PCR surveillance of the rstR genes of CTX phages in Vibrio cholerae O1 and O139 showed no relationship between the incidence of disease and changes in the rstR but showed variations in their presence...

Nontoxic Shiga Toxin Derivatives from Escherichia coli Possess Adjuvant Activity for the Augmentation of Antigen-Specific Immune Responses via Dendritic Cell Activation

Ohmura, Mari, Yamamoto, Masafumi, Tomiyama-Miyaji, Chikako, Yuki, Yoshikazu, Takeda, Yoshifumi, Kiyono, Hiroshi

Shiga toxin (Stx) derivatives, such as the Stx1 B subunit (StxB1), which mediates toxin binding to the membrane, and mutant Stx1 (mStx1), which is a nontoxic doubly mutated Stx1 harboring amino acid...

A nontoxic mutant of cholera toxin elicits Th2-type responses for enhanced mucosal immunity

Yamamoto, Shingo, Kiyono, Hiroshi, Yamamoto, Masafumi, Imaoka, Koichi, Yamamoto, Miho, Fujihashi, Kohtaro, ...

We have characterized a nontoxic mutant of cholera toxin (CT) as a mucosal adjuvant in mice. The mutant CT was made by substitution of serine with phenylalanine at position 61 of the A subunit...

A 1.2-Å snapshot of the final step of bacterial cell wall biosynthesis

Lee, Wenlin, McDonough, Michael A., Kotra, Lakshmi P., Li, Zhi-Hong, Silvaggi, Nicholas R., Takeda, Yoshifumi, ...

The cell wall imparts structural strength and shape to bacteria. It is made up of polymeric glycan chains with peptide branches that are cross-linked to form the cell wall. The cross-linking...

Clonal Diversity among Recently Emerged Strains of Vibrio parahaemolyticus O3:K6 Associated With Pandemic Spread

Bag, Prasanta K., Nandi, Suvobroto, Bhadra, Rupak K., Ramamurthy, T., Bhattacharya, S. K., Nishibuchi, M., ...

The genomes of the O3:K6 strains of Vibrio parahaemolyticus which abruptly emerged in Calcutta, India, in February 1996 and which demonstrated an unusual potential to spread and an enhanced...

Emergence of Vibrio cholerae O1 Biotype El Tor Serotype Inaba from the Prevailing O1 Ogawa Serotype Strains in India

Garg, Pallavi, Nandy, Ranjan K., Chaudhury, Papiya, Chowdhury, Nandini Roy, De, Keya, Ramamurthy, T., ...

The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular...

Virulence Genes in Environmental Strains of Vibrio cholerae

Chakraborty, Soumen, Mukhopadhyay, Asish K., Bhadra, Rupak Kumar, Ghosh, Amar Nath, Mitra, Rupak, Shimada, Toshio, ...

The virulence of a pathogen is dependent on a discrete set of genetic determinants and their well-regulated expression. The ctxAB and tcpA genes are known to play a cardinal role in maintaining...

Cell Vacuolation, a Manifestation of the El Tor Hemolysin of Vibrio cholerae

Mitra, Rupak, Figueroa, Paula, Mukhopadhyay, Asish K., Shimada, Toshio, Takeda, Yoshifumi, Berg, Douglas E., ...

Culture supernatants of nontoxigenic nonepidemic clinical strains of Vibrio cholerae belonging to diverse serogroups were found to induce vacuolation of nonconfluent HeLa cells. The vacuoles became...

Protective Effect of Lactobacillus casei Strain Shirota on Shiga Toxin-Producing Escherichia coli O157:H7 Infection in Infant Rabbits

Ogawa, Michinaga, Shimizu, Kensuke, Nomoto, Koji, Takahashi, Masatoshi, Watanuki, Masaaki, Tanaka, Ryuichiro, ...

We examined colonization patterns of Shiga toxin-producing Escherichia coli (STEC), concentrations of Shiga toxins (Stxs) and specific immunoglobulin A (lgA) against Stxs and STEC bacterial cell...

Characterization of VPI Pathogenicity Island and CTXφ Prophage in Environmental Strains of Vibrio cholerae

Mukhopadhyay, Asish K., Chakraborty, Soumen, Takeda, Yoshifumi, Nair, G. Balakrish, Berg, Douglas E.

Environmental isolates of Vibrio cholerae of eight randomly amplified polymorphic DNA (RAPD) fingerprint types from Calcutta, India, that were unusual in containing toxin-coregulated pilus or cholera...

Molecular Epidemiology of Reemergent Vibrio cholerae O139 Bengal in India

Mukhopadhyay, Asish Kumar, Basu, Arnab, Garg, Pallavi, Bag, Prasanta K., Ghosh, Amit, Bhattacharya, S. K., ...

We report the prevalence of the O139 serogroup in Calcutta, India, after its reemergence in August 1996 and the spread of the reemerged clone to other parts of the country by using previously...

Identification and Characterization of a Newly Isolated Shiga Toxin 2-Converting Phage from Shiga Toxin-Producing Escherichia coli

Watarai, Masahisa, Sato, Toshio, Kobayashi, Midori, Shimizu, Takeshi, Yamasaki, Shinji, Tobe, Toru, ...

Shiga toxins 1 (Stx1) and 2 (Stx2) are encoded by toxin-converting bacteriophages of Stx-producing Escherichia coli (STEC), and so far two Stx1- and one Stx2-converting phages have been isolated from...

Mutational Analysis of Superantigen Activity Responsible for the Induction of Skin Erythema by Streptococcal Pyrogenic Exotoxin C

Yamaoka, Junichi, Nakamura, Eijiro, Takeda, Yoshifumi, Imamura, Sadao, Minato, Nagahiro

Streptococcal pyrogenic exotoxin C (SPEC), when injected intradermally, induces erythema in unsensitized rabbits. In the present study, we examined whether this erythema induction is due to the...

Prevalence of the Pandemic Genotype of Vibrio parahaemolyticus in Dhaka, Bangladesh, and Significance of Its Distribution across Different Serotypes

Bhuiyan, N. A., Ansaruzzaman, M., Kamruzzaman, M., Alam, Khorshed, Chowdhury, N. R., Nishibuchi, M., ...

Sixty-six strains of Vibrio parahaemolyticus belonging to 14 serotypes were isolated from hospitalized patients in Dhaka, Bangladesh, from January 1998 to December 2000. Among these, 48 strains...

Escalating Association of Vibrio cholerae O139 with Cholera Outbreaks in India

Sinha, Sutapa, Chakraborty, Rupa, De, Keya, Khan, Asis, Datta, Simanti, Ramamurthy, T., ...

Between December 1999 and December 2000, teams from the National Institute of Cholera and Enteric Diseases, Calcutta, India, examined eight outbreaks of cholera, which occurred in different parts of...

Isolation of Shigella dysenteriae Type 1 and S. flexneri Strains from Surface Waters in Bangladesh: Comparative Molecular Analysis of Environmental Shigella Isolates versus Clinical Strains

Faruque, Shah M., Khan, Rasel, Kamruzzaman, M., Yamasaki, Shinji, Ahmad, Q. Shafi, Azim, Tasnim, ...

Bacillary dysentery caused by Shigella species is a public health problem in developing countries including Bangladesh. Although, shigellae-contaminated food and drinks are often the source of the...

Cloning and Characterization of Genes Encoding Homologues of the B Subunit of Cholera Toxin and the Escherichia coli Heat-Labile Enterotoxin from Clinical Isolates of Citrobacter freundii and E. coli

Karasawa, Tadahiro, Ito, Hideaki, Tsukamoto, Teizo, Yamasaki, Shinji, Kurazono, Hisao, Faruque, Shah M., ...

We identified and characterized a gene encoding a homologue of the B subunits of cholera toxin (CTB) and heat-labile enterotoxin (LTB) of Escherichia coli from a clinical isolate of Citrobacter...

Genome Analysis of a Novel Shiga Toxin 1 (Stx1)-Converting Phage Which Is Closely Related to Stx2-Converting Phages but Not to Other Stx1-Converting Phages

Sato, Toshio, Shimizu, Takeshi, Watarai, Masahisa, Kobayashi, Midori, Kano, Shigeyuki, Hamabata, Takashi, ...

Two Stx-converting phages, designated Stx1φ and Stx2φ-II, were isolated from an Escherichia coli O157:H7 strain, Morioka V526, and their entire nucleotide sequences were determined. The genomes of...

Antigenic Difference Between Polar Monotrichous and Peritrichous Flagella of Vibrio parahaemolyticus

Shinoda, Sumio, Honda, Takeshi, Takeda, Yoshifumi, Miwatani, Toshio

Polar monotrichous and peritrichous flagella of Vibrio parahaemolyticus were isolated and purified separately. On hydroxylapatite column chromatography, the flagellins of polar monotrichous flagella...

Characterization of Aminoacyl Transfer Ribonucleic Acid Formation Stimulated by Polyamines

Takeda, Yoshifumi, Matsuzaki, Kazuyoshi, Igarashi, Kazuei

From a comparative study of aminoacyl transfer ribonucleic acid (tRNA) formation stimulated by polyamines or by Mg2+, it is shown that both reactions have the same requirements for adenosine...

Association of Cytolethal Distending Toxin Locus cdtB with Enteropathogenic Escherichia coli Isolated from Patients with Acute Diarrhea in Calcutta, India

Pandey, Mritunjay, Khan, Asis, Das, Suresh C., Sarkar, Bhaswati, Kahali, Soumen, Chakraborty, Subhra, ...

Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These...

Emergence and evolution of Vibrio cholerae O139

Faruque, Shah M., Sack, David A., Sack, R. Bradley, Colwell, Rita R., Takeda, Yoshifumi, Nair, G. Balakrish

The emergence of Vibrio cholerae O139 Bengal during 1992–1993 was associated with large epidemics of cholera in India and Bangladesh and, initially, with a total displacement of the existing V....

Clonal Multidrug-Resistant Shigella dysenteriae Type 1 Strains Associated with Epidemic and Sporadic Dysenteries in Eastern India

Pazhani, Gururaja Perumal, Sarkar, Bhaswati, Ramamurthy, Thandavarayan, Bhattacharya, S. K., Takeda, Yoshifumi, Niyogi, S. K.

Multidrug-resistant strains of Shigella dysenteriae type 1 were implicated in three outbreaks and sporadic cases of dysentery in eastern India in 2002 and 2003. After a hiatus of 14 years, this...

Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7

Asahara, Takashi, Shimizu, Kensuke, Nomoto, Koji, Hamabata, Takashi, Ozawa, Ayako, Takeda, Yoshifumi

The anti-infectious activity of probiotic Bifidobacteria against Shiga toxin-producing Escherichia coli (STEC) O157:H7 was examined in a fatal mouse STEC infection model. Stable colonization of the...

Existence of Two Distinct Hemolysins in Vibrio parahaemolyticus

Sakurai, Jun, Matsuzaki, Akiko, Takeda, Yoshifumi, Miwatani, Toshio

Two distinct hemolysins were demonstrated in Vibrio parahaemolyticus. A thermostable direct hemolysin purified from V. parahemolyticus WP-1, a Kanagawa phenomenon (KP)-positive strain, is...

Demonstration of a Temperature-Dependent Inactivating Factor of the Thermostable Direct Hemolysin in Vibrio parahaemolyticus

Takeda, Yoshifumi, Hori, Yayoi, Miwatani, Toshio

A factor was found in Vibrio parahaemolyticus which inactivated the hemolytic activity of a purified, thermostable direct hemolysin. The inactivating factor was associated with the hemolysin but...