Shimizu, Masato, Hiroaki, Hidekazu, Kohda, Daisuke, Hosoya, Toshihiko, Akiyama-Oda, Yasuko, Hotta, Yoshiki, ...
Drosophila GCM (glial cell missing) is a novel DNA-binding protein that determines the fate of glial precursors from the neural default to glia. The GCM protein contains the functional domain that is...
Kobayashi, Hisao, Inoue, Akihiro, Mikawa, Takashi, Kuwayama, Hideto, Hotta, Yoshiki, Masaki, Tomoh, ...
Two proteins with myosin light chain kinase activity and electrophoretic molecular weights of 155,000 and 130,000 were each isolated from bovine stomach smooth muscle [Kuwayama, H., Suzuki, M., Koga,...
The Drosophila mutant no receptor potential A (norpA) is the phototransduction-defective mutant which lacks phosphatidylinositol-specific phospholipase C (PI-PLC) activity. Recently, norpA cDNA was...
Sakai, Yutaka, Okamoto, Hitoshi, Mogami, Kaname, Matsuo, Hiroki, Hotta, Yoshiki
Artificially mutagenized Drosophila Act88F actin genes with triple and double mutations were expressed in the indirect flight muscles of transgenic flies. The triple mutant actin, GD246T...
Sakai, Yutaka, Okamoto, Hitoshi, Mogami, Kaname, Yamada, Takuma, Hotta, Yoshiki
A mutant β-actin with an amino acid substitution from Gly-245 to Asp has been shown to be related to tumorigenic transformation of a human fibroblast cell line (Leavitt, J. et al (1987)Mol. Cell...
Suzuki, Emiko, Hirosawa, Kazushige, Hotta, Yoshiki
A Drosophila visual mutant, rdgA, has photoreceptor cells whose rhabdomeres degenerate in several days after eclosion. Incorporations of 3H-amino acids, and 3H-mannose and 3H-glucosamine residues...
Membrane-Associated Phospholipase C of Drosophila Retina (1988)
Inoue, Hiroko, Yoshioka, Tohru, Hotta, Yoshiki
Phospholipase C activities against phosphatidylinositol 4,5-bisphosphate and phos-phatidylinositol have been examined using head homogenate of Drosophila visual mutants. In many mutants the enzyme...
Evidence that Arachidonic Acid Is Deficient in Phosphatidylinositol of Drosophila Heads (1985)
YOSHIOKA, Tohru, INOUE, Hiroko, KASAMA, Takeshi, SEYAMA, Yousuke, NAKASHIMA, Shigeru, NOZAWA, Yoshinori, ...
We have found that arachidonic (20 : 4) acid is indetectable in phosphatidylinositol and diacylglycerol extracted from Drosophila heads. After careful examinations of the lipid extraction processes...
YOSHIOKA, Tohru, INOUE, Hiroko, HOTTA, Yoshiki
Phosphatidylinositol phosphodiesterase activity was found to be entirely absent in the head of the Drosophila visual mutant, norpA. It was also demonstrated that the enzyme activity was highly...
MOGAMI, Kaname, FUJITA, Shinobu C., HOTTA, Yoshiki
When proteins of whole Drosophila thorax were analyzed by two-dimensional gel electrophoresis, 186 spots were detected by protein staining with Coomassie brilliant blue R-250. Two methods were...
Courtship in Drosophila Mosaics: Sex-Specific Foci for Sequential Action Patterns (1976)
Hotta, Yoshiki, Benzer, Seymour
Mosaic fate mapping is used to locate the foci determining sex-specific steps in the mating behavior of Drosophila. Male performance of following females and displaying wing vibration toward them...
Genetic Dissection of the Drosophila Nervous System by means of Mosaics (1970)
Hotta, Yoshiki, Benzer, Seymour
Given a mutant having abnormal behavior, the anatomical domain responsible for the deficit may be identified by the use of genetic mosaicism. Individuals may be produced in which a portion of the...
The gcm-motif: A novel DNA-binding motif conserved in Drosophila and mammals
Akiyama, Yasuko, Hosoya, Toshihiko, Poole, Anthony M., Hotta, Yoshiki
In the Drosophila nervous system, the glial cells missing gene (gcm) is transiently expressed in glial precursors to switch their fate from the neuronal default to glia. It encodes a novel 504-amino...
Genetic Dissection of the Drosophila Nervous System by Means of Mosaics
Hotta, Yoshiki, Benzer, Seymour
Given a mutant having abnormal behavior, the anatomical domain responsible for the deficit may be identified by the use of genetic mosaicism. Individuals may be produced in which a portion of the...
Actin gene mutations in Drosophila; heat shock activation in the indirect flight muscles
Hiromi, Yasushi, Hotta, Yoshiki
We have identified four mutations affecting the actin III isoform in the indirect flight muscles (IFM) of Drosophila. One mutation does not produce any protein product, and three direct the synthesis...
Okamoto, Hitoshi, Hiromi, Yasushi, Ishikawa, Etsuko, Yamada, Takuma, Isoda, Kazuyoshi, Maekawa, Hideaki, ...
Heat-shock proteins (hsps) are constitutively induced by the mutant actins in the Drosophila indirect flight muscles (IFM). We compared primary structures of the mutant actin genes (KM75 and HH5)...
The gcm-motif: A novel DNA-binding motif conserved in Drosophila and mammals
Akiyama, Yasuko, Hosoya, Toshihiko, Poole, Anthony M., Hotta, Yoshiki
In the Drosophila nervous system, the glial cells missing gene (gcm) is transiently expressed in glial precursors to switch their fate from the neuronal default to glia. It encodes a novel 504-amino...
Genetic Dissection of the Drosophila Nervous System by Means of Mosaics
Hotta, Yoshiki, Benzer, Seymour
Given a mutant having abnormal behavior, the anatomical domain responsible for the deficit may be identified by the use of genetic mosaicism. Individuals may be produced in which a portion of the...
Actin gene mutations in Drosophila; heat shock activation in the indirect flight muscles
Hiromi, Yasushi, Hotta, Yoshiki
We have identified four mutations affecting the actin III isoform in the indirect flight muscles (IFM) of Drosophila. One mutation does not produce any protein product, and three direct the synthesis...
Okamoto, Hitoshi, Hiromi, Yasushi, Ishikawa, Etsuko, Yamada, Takuma, Isoda, Kazuyoshi, Maekawa, Hideaki, ...
Heat-shock proteins (hsps) are constitutively induced by the mutant actins in the Drosophila indirect flight muscles (IFM). We compared primary structures of the mutant actin genes (KM75 and HH5)...