Hu, Guowu, Hacham, Moshe, Waterman, Scott R., Panepinto, John, Shin, Soowan, Liu, Xiaoguang, ...
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Structure of Atg5.Atg16, a complex essential for autophagy. (2007)
Matsushita, Minako, Suzuki, Nobuo N., Obara, Keisuke, Fujioka, Yuko, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
Atg5 is covalently modified with a ubiquitin-like modifier, Atg12, and the Atg12-Atg5 conjugate further forms a complex with the multimeric protein Atg16. The Atg12-Atg5·Atg16 multimeric complex...
Structure of Atg5.Atg16, a complex essential for autophagy. (2007)
Matsushita, Minako, Suzuki, Nobuo N., Obara, Keisuke, Fujioka, Yuko, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
Atg5 is covalently modified with a ubiquitin-like modifier, Atg12, and the Atg12-Atg5 conjugate further forms a complex with the multimeric protein Atg16. The Atg12-Atg5·Atg16 multimeric complex...
Inoue, Yuko, Suzuki, Takao, Hattori, Masaki, Yoshimoto, Kohki, Ohsumi, Yoshinori, Moriyasu, Yuji
In Arabidopsis root tips cultured in medium containing sufficient nutrients and the membrane-permeable protease inhibitor E-64d, parts of the cytoplasm accumulated in the vacuoles of the cells from...
Inoue, Yuko, Suzuki, Takao, Hattori, Masaki, Yoshimoto, Kohki, Ohsumi, Yoshinori, Moriyasu, Yuji
In Arabidopsis root tips cultured in medium containing sufficient nutrients and the membrane-permeable protease inhibitor E-64d, parts of the cytoplasm accumulated in the vacuoles of the cells from...
A Unified Nomenclature for Yeast Autophagy-Related Genes (2003)
Klionsky, Daniel J., Cregg, James M., Emr, Scott D., Sakai, Yasuyoshi, Sandoval, Ignacio V., ...
Suzuki, Takashi, Nakagawa, Masahiro, Yoshikawa, Ayumu, Sasagawa, Noboru, Yoshimori, Tamotsu, Ohsumi, Yoshinori, ...
Chloroquine myopathy is a drug poisoning disease involving rimmed vacuole formation. By Western blot analysis, we investigated posttranslational modification of LC3 in cultured cells with a high...
Scott, Sidney V., Nice III, Daniel C., Nau, Johnathan J., Weisman, Lois S., Kamada, Yoshiaki, Keizer-Gunnink, Ineke, ...
We have been studying protein components that function in the cytoplasm to vacuole targeting (Cvt) pathway and the overlapping process of macroautophagy. The Vac8 and Apg13 proteins are required for...
Nakamura, Norihiro, Matsuura, Akira, Wada, Yoh, Ohsumi, Yoshinori
Acidification inside vacuoles has been shown to play a key role in a number of physiologically important cellular events. We studied the role of vacuolar membrane H+-ATPase in the autophagic process...
Vacuolar Function in the Phosphate Homeostasis of the Yeast Saccharomyces cerevisiae (1996)
Shirahama, Kanae, Yazaki, Yoshiaki, Sakano, Katsuhiro, Wada, Yoh, Ohsumi, Yoshinori
We studied physiological roles of the yeast vacuole in the phosphate metabolism using 31P-in vivo nuclear magnetic resonance (NMR) spectroscopy. Under phosphate starvation wild-type yeast cells...
Konishi, Jin, Denda, Kimitoshi, Oshima, Tairo, Wakagi, Takayoshi, Uchida, Etsuko, Ohsumi, Yoshinori, ...
Immunological cross-reactivity among three types of H+-ATPases, that is, three archaebacterial ATPases, the F1-ATPase from thermophilic bacterium PS3 (TF1 and the vacuolar membrane ATPase from...
Yoshimori, Tamotsu, Yamagata, Fumi, Yamamoto, Akitsugu, Mizushima, Noboru, Kabeya, Yukiko, Nara, Atsuki, ...
The mouse SKD1 is an AAA-type ATPase homologous to the yeast Vps4p implicated in transport from endosomes to the vacuole. To elucidate a possible role of SKD1 in mammalian endocytosis, we generated a...
George, Michael D., Baba, Misuzu, Scott, Sidney V., Mizushima, Noboru, Garrison, Brian S., Ohsumi, Yoshinori, ...
The cytoplasm-to-vacuole targeting (Cvt) pathway and macroautophagy are dynamic events involving the rearrangement of membrane to form a sequestering vesicle in the cytosol, which subsequently...
Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy
Tanida, Isei, Mizushima, Noboru, Kiyooka, Miho, Ohsumi, Mariko, Ueno, Takashi, Ohsumi, Yoshinori, ...
In the yeast Saccharomyces cerevisiae, the Apg12p–Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate...
Ishihara, Naotada, Hamasaki, Maho, Yokota, Sadaki, Suzuki, Kuninori, Kamada, Yoshiaki, Kihara, Akio, ...
Double membrane structure, autophagosome, is formed de novo in the process of autophagy in the yeast Saccharomyces cerevisiae, and many Apg proteins participate in this process. To further understand...
Suzuki, Kuninori, Kirisako, Takayoshi, Kamada, Yoshiaki, Mizushima, Noboru, Noda, Takeshi, Ohsumi, Yoshinori
Macroautophagy is a bulk degradation process induced by starvation in eukaryotic cells. In yeast, 15 Apg proteins coordinate the formation of autophagosomes. Several key reactions performed by these...
Hanaoka, Hideki, Noda, Takeshi, Shirano, Yumiko, Kato, Tomohiko, Hayashi, Hiroaki, Shibata, Daisuke, ...
Autophagy is an intracellular process for vacuolar bulk degradation of cytoplasmic components. The molecular machinery responsible for yeast and mammalian autophagy has recently begun to be...
Tsugawa, Akiko, Ohsumi, Yoshinori
The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger...
Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene
Qu, Xueping, Yu, Jie, Bhagat, Govind, Furuya, Norihiko, Hibshoosh, Hanina, Troxel, Andrea, ...
Malignant cells often display defects in autophagy, an evolutionarily conserved pathway for degrading long-lived proteins and cytoplasmic organelles. However, as yet, there is no genetic evidence for...
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Kabeya, Yukiko, Mizushima, Noboru, Ueno, Takashi, Yamamoto, Akitsugu, Kirisako, Takayoshi, Noda, Takeshi, ...
Little is known about the protein constituents of autophagosome membranes in mammalian cells. Here we demonstrate that the rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of...
Mukaiyama, Hiroyuki, Baba, Misuzu, Osumi, Masako, Aoyagi, Satoshi, Kato, Nobuo, Ohsumi, Yoshinori, ...
Microautophagy is a versatile process in which vacuolar or lysosomal membranes directly sequester cytosolic targets for degradation. Recent genetic evidence suggested that microautophagy uses...
Mizushima, Noboru, Yamamoto, Akitsugu, Matsui, Makoto, Yoshimori, Tamotsu, Ohsumi, Yoshinori
Macroautophagy mediates the bulk degradation of cytoplasmic components. It accounts for the degradation of most long-lived proteins: cytoplasmic constituents, including organelles, are sequestered...
Studies on a Factor Enhancing Colicin E3 Activity In Vitro
Ohsumi, Yoshinori, Imahori, Kazutomo
The mechanism of action of colicin E3 (E3) was investigated in an in vitro system. Purified ribosomes are less susceptible to E3 than crude or washed ribosomes. A factor was found in the supernatant...
Yoshimoto, Kohki, Hanaoka, Hideki, Sato, Shusei, Kato, Tomohiko, Tabata, Satoshi, Noda, Takeshi, ...
Autophagy is an intracellular process for vacuolar degradation of cytoplasmic components. Thus far, plant autophagy has been studied primarily using morphological analyses. A recent genome-wide...
Ano, Yoshitaka, Hattori, Takeshi, Oku, Masahide, Mukaiyama, Hiroyuki, Baba, Misuzu, Ohsumi, Yoshinori, ...
Diverse cellular processes such as autophagic protein degradation require phosphoinositide signaling in eukaryotic cells. In the methylotrophic yeast Pichia pastoris, peroxisomes can be selectively...
Beclin–phosphatidylinositol 3-kinase complex functions at the trans-Golgi network
Kihara, Akio, Kabeya, Yukiko, Ohsumi, Yoshinori, Yoshimori, Tamotsu
Autophagy is an intracellular bulk protein degradation system. Beclin is known to be involved in this process; however, its role is unclear. In this study, we showed that Beclin was...
Atg17 Functions in Cooperation with Atg1 and Atg13 in Yeast Autophagy
Kabeya, Yukiko, Kamada, Yoshiaki, Baba, Misuzu, Takikawa, Hirosato, Sasaki, Mitsuru, Ohsumi, Yoshinori
In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes...
Tor2 Directly Phosphorylates the AGC Kinase Ypk2 To Regulate Actin Polarization†
Kamada, Yoshiaki, Fujioka, Yuko, Suzuki, Nobuo N., Inagaki, Fuyuhiko, Wullschleger, Stephan, Loewith, Robbie, ...
The target of rapamycin (TOR) protein kinases, Tor1 and Tor2, form two distinct complexes (TOR complex 1 and 2) in the yeast Saccharomyces cerevisiae. TOR complex 2 (TORC2) contains Tor2 but not Tor1...
Obara, Keisuke, Sekito, Takayuki, Ohsumi, Yoshinori
In the yeast Saccharomyces cerevisiae, two similar phosphatidylinositol 3-kinase complexes (complexes I and II) function in distinct biological processes, complex I in autophagy and complex II in the...
Yoshimori, Tamotsu, Yamagata, Fumi, Yamamoto, Akitsugu, Mizushima, Noboru, Kabeya, Yukiko, Nara, Atsuki, ...
The mouse SKD1 is an AAA-type ATPase homologous to the yeast Vps4p implicated in transport from endosomes to the vacuole. To elucidate a possible role of SKD1 in mammalian endocytosis, we generated a...
George, Michael D., Baba, Misuzu, Scott, Sidney V., Mizushima, Noboru, Garrison, Brian S., Ohsumi, Yoshinori, ...
The cytoplasm-to-vacuole targeting (Cvt) pathway and macroautophagy are dynamic events involving the rearrangement of membrane to form a sequestering vesicle in the cytosol, which subsequently...
Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy
Tanida, Isei, Mizushima, Noboru, Kiyooka, Miho, Ohsumi, Mariko, Ueno, Takashi, Ohsumi, Yoshinori, ...
In the yeast Saccharomyces cerevisiae, the Apg12p–Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate...
Ishihara, Naotada, Hamasaki, Maho, Yokota, Sadaki, Suzuki, Kuninori, Kamada, Yoshiaki, Kihara, Akio, ...
Double membrane structure, autophagosome, is formed de novo in the process of autophagy in the yeast Saccharomyces cerevisiae, and many Apg proteins participate in this process. To further understand...
Suzuki, Kuninori, Kirisako, Takayoshi, Kamada, Yoshiaki, Mizushima, Noboru, Noda, Takeshi, Ohsumi, Yoshinori
Macroautophagy is a bulk degradation process induced by starvation in eukaryotic cells. In yeast, 15 Apg proteins coordinate the formation of autophagosomes. Several key reactions performed by these...
Hanaoka, Hideki, Noda, Takeshi, Shirano, Yumiko, Kato, Tomohiko, Hayashi, Hiroaki, Shibata, Daisuke, ...
Autophagy is an intracellular process for vacuolar bulk degradation of cytoplasmic components. The molecular machinery responsible for yeast and mammalian autophagy has recently begun to be...
Tsugawa, Akiko, Ohsumi, Yoshinori
The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger...
Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene
Qu, Xueping, Yu, Jie, Bhagat, Govind, Furuya, Norihiko, Hibshoosh, Hanina, Troxel, Andrea, ...
Malignant cells often display defects in autophagy, an evolutionarily conserved pathway for degrading long-lived proteins and cytoplasmic organelles. However, as yet, there is no genetic evidence for...
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Kabeya, Yukiko, Mizushima, Noboru, Ueno, Takashi, Yamamoto, Akitsugu, Kirisako, Takayoshi, Noda, Takeshi, ...
Little is known about the protein constituents of autophagosome membranes in mammalian cells. Here we demonstrate that the rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of...
Mukaiyama, Hiroyuki, Baba, Misuzu, Osumi, Masako, Aoyagi, Satoshi, Kato, Nobuo, Ohsumi, Yoshinori, ...
Microautophagy is a versatile process in which vacuolar or lysosomal membranes directly sequester cytosolic targets for degradation. Recent genetic evidence suggested that microautophagy uses...
Mizushima, Noboru, Yamamoto, Akitsugu, Matsui, Makoto, Yoshimori, Tamotsu, Ohsumi, Yoshinori
Macroautophagy mediates the bulk degradation of cytoplasmic components. It accounts for the degradation of most long-lived proteins: cytoplasmic constituents, including organelles, are sequestered...
Studies on a Factor Enhancing Colicin E3 Activity In Vitro
Ohsumi, Yoshinori, Imahori, Kazutomo
The mechanism of action of colicin E3 (E3) was investigated in an in vitro system. Purified ribosomes are less susceptible to E3 than crude or washed ribosomes. A factor was found in the supernatant...
Yoshimoto, Kohki, Hanaoka, Hideki, Sato, Shusei, Kato, Tomohiko, Tabata, Satoshi, Noda, Takeshi, ...
Autophagy is an intracellular process for vacuolar degradation of cytoplasmic components. Thus far, plant autophagy has been studied primarily using morphological analyses. A recent genome-wide...
Ano, Yoshitaka, Hattori, Takeshi, Oku, Masahide, Mukaiyama, Hiroyuki, Baba, Misuzu, Ohsumi, Yoshinori, ...
Diverse cellular processes such as autophagic protein degradation require phosphoinositide signaling in eukaryotic cells. In the methylotrophic yeast Pichia pastoris, peroxisomes can be selectively...
Beclin–phosphatidylinositol 3-kinase complex functions at the trans-Golgi network
Kihara, Akio, Kabeya, Yukiko, Ohsumi, Yoshinori, Yoshimori, Tamotsu
Autophagy is an intracellular bulk protein degradation system. Beclin is known to be involved in this process; however, its role is unclear. In this study, we showed that Beclin was...
Atg17 Functions in Cooperation with Atg1 and Atg13 in Yeast Autophagy
Kabeya, Yukiko, Kamada, Yoshiaki, Baba, Misuzu, Takikawa, Hirosato, Sasaki, Mitsuru, Ohsumi, Yoshinori
In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes...
Tor2 Directly Phosphorylates the AGC Kinase Ypk2 To Regulate Actin Polarization†
Kamada, Yoshiaki, Fujioka, Yuko, Suzuki, Nobuo N., Inagaki, Fuyuhiko, Wullschleger, Stephan, Loewith, Robbie, ...
The target of rapamycin (TOR) protein kinases, Tor1 and Tor2, form two distinct complexes (TOR complex 1 and 2) in the yeast Saccharomyces cerevisiae. TOR complex 2 (TORC2) contains Tor2 but not Tor1...
Obara, Keisuke, Sekito, Takayuki, Ohsumi, Yoshinori
In the yeast Saccharomyces cerevisiae, two similar phosphatidylinositol 3-kinase complexes (complexes I and II) function in distinct biological processes, complex I in autophagy and complex II in the...
Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy
Amar, Nira, Lustig, Gila, Ichimura, Yoshinobu, Ohsumi, Yoshinori, Elazar, Zvulun
Atg8, a member of a novel ubiquitin-like protein family, is an essential component of the autophagic machinery in yeast. This protein undergoes reversible conjugation to phosphatidylethanolamine...
An Arabidopsis Homolog of Yeast ATG6/VPS30 Is Essential for Pollen Germination1[W]
Fujiki, Yuki, Yoshimoto, Kohki, Ohsumi, Yoshinori
Yeast (Saccharomyces cerevisiae) Atg6/Vps30 is required for autophagy and the sorting of vacuolar hydrolases, such as carboxypeptidase Y. In higher eukaryotes, however, roles for ATG6/VPS30 homologs...
Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice
Komatsu, Masaaki, Waguri, Satoshi, Ueno, Takashi, Iwata, Junichi, Murata, Shigeo, Tanida, Isei, ...
Autophagy is a membrane-trafficking mechanism that delivers cytoplasmic constituents into the lysosome/vacuole for bulk protein degradation. This mechanism is involved in the preservation of...
Hu, Guowu, Hacham, Moshe, Waterman, Scott R., Panepinto, John, Shin, Soowan, Liu, Xiaoguang, ...
Autophagy is a process by which cells recycle cytoplasm and defective organelles during stress situations such as nutrient starvation. It can also be used by host cells as an immune defense mechanism...
Two Distinct Pathways for Targeting Proteins from the Cytoplasm to the Vacuole/Lysosome
Baba, Misuzu, Osumi, Masako, Scott, Sidney V., Klionsky, Daniel J., Ohsumi, Yoshinori
Stress conditions lead to a variety of physiological responses at the cellular level. Autophagy is an essential process used by animal, plant, and fungal cells that allows for both recycling of...
Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism
Scott, Sidney V., Baba, Misuzu, Ohsumi, Yoshinori, Klionsky, Daniel J.
The yeast vacuolar protein aminopeptidase I (API) is synthesized as a cytosolic precursor that is transported to the vacuole by a nonclassical targeting mechanism. Recent genetic studies indicate...
Tor-Mediated Induction of Autophagy via an Apg1 Protein Kinase Complex
Kamada, Yoshiaki, Funakoshi, Tomoko, Shintani, Takahiro, Nagano, Kazuya, Ohsumi, Mariko, Ohsumi, Yoshinori
Autophagy is a membrane trafficking to vacuole/lysosome induced by nutrient starvation. In Saccharomyces cerevisiae, Tor protein, a phosphatidylinositol kinase-related kinase, is involved in the...
Cvt9/Gsa9 Functions in Sequestering Selective Cytosolic Cargo Destined for the Vacuole
Kim, John, Kamada, Yoshiaki, Stromhaug, Per E., Guan, Ju, Hefner-Gravink, Ann, Baba, Misuzu, ...
Three overlapping pathways mediate the transport of cytoplasmic material to the vacuole in Saccharomyces cerevisiae. The cytoplasm to vacuole targeting (Cvt) pathway transports the vacuolar...
Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
Kirisako, Takayoshi, Baba, Misuzu, Ishihara, Naotada, Miyazawa, Kouichi, Ohsumi, Mariko, Yoshimori, Tamotsu, ...
We characterized Apg8/Aut7p essential for autophagy in yeast. Apg8p was transcriptionally upregulated in response to starvation and mostly existed as a protein bound to membrane under both growing...
Noda, Takeshi, Kim, John, Huang, Wei-Pang, Baba, Misuzu, Tokunaga, Chikara, Ohsumi, Yoshinori, ...
In nutrient-rich, vegetative conditions, the yeast Saccharomyces cerevisiae transports a resident protease, aminopeptidase I (API), to the vacuole by the cytoplasm to vacuole targeting (Cvt) pathway,...
Geranylgeranylated Snares Are Dominant Inhibitors of Membrane Fusion
Grote, Eric, Baba, Misuzu, Ohsumi, Yoshinori, Novick, Peter J.
Exocytosis in yeast requires the assembly of the secretory vesicle soluble N-ethylmaleimide–sensitive factor attachment protein receptor (v-SNARE) Sncp and the plasma membrane t-SNAREs Ssop and...
Kirisako, Takayoshi, Ichimura, Yoshinobu, Okada, Hisashi, Kabeya, Yukiko, Mizushima, Noboru, Yoshimori, Tamotsu, ...
Autophagy and the Cvt pathway are examples of nonclassical vesicular transport from the cytoplasm to the vacuole via double-membrane vesicles. Apg8/Aut7, which plays an important role in the...
Dissection of Autophagosome Formation Using Apg5-Deficient Mouse Embryonic Stem Cells
Mizushima, Noboru, Yamamoto, Akitsugu, Hatano, Masahiko, Kobayashi, Yoshinori, Kabeya, Yukiko, Suzuki, Kuninori, ...
In macroautophagy, cytoplasmic components are delivered to lysosomes for degradation via autophagosomes that are formed by closure of cup-shaped isolation membranes. However, how the isolation...
Kihara, Akio, Noda, Takeshi, Ishihara, Naotada, Ohsumi, Yoshinori
Vps30p/Apg6p is required for both autophagy and sorting of carboxypeptidase Y (CPY). Although Vps30p is known to interact with Apg14p, its precise role remains unclear. We found that two proteins...
The Yeast Tor Signaling Pathway Is Involved in G2/M Transition via Polo-Kinase
Nakashima, Akio, Maruki, Yoshiko, Imamura, Yuko, Kondo, Chika, Kawamata, Tomoko, Kawanishi, Ippei, ...
The target of rapamycin (Tor) protein plays central roles in cell growth. Rapamycin inhibits cell growth and promotes cell cycle arrest at G1 (G0). However, little is known about whether Tor is...
Organization of the Pre-autophagosomal Structure Responsible for Autophagosome Formation
Kawamata, Tomoko, Kamada, Yoshiaki, Kabeya, Yukiko, Sekito, Takayuki, Ohsumi, Yoshinori
Autophagy induced by nutrient depletion is involved in survival during starvation conditions. In addition to starvation-induced autophagy, the yeast Saccharomyces cerevisiae also has a constitutive...
Crystallization of the Atg12–Atg5 conjugate bound to Atg16 by the free-interface diffusion method
Noda, Nobuo N., Fujioka, Yuko, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
The Atg12–Atg5 conjugate was prepared by in vivo reconstitution and was crystallized with Atg16 using the free-interface diffusion method.
Crystallization and preliminary X-ray analysis of Atg3
Yamada, Yuya, Suzuki, Nobuo N., Fujioka, Yuko, Ichimura, Yoshinobu, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
S. cerevisiae Atg3, an E2-like enzyme that mediates the lipidation of Atg8, was crystallized and diffracted to 2.5 Å resolution.
Expression, purification and crystallization of the Atg5–Atg16 complex essential for autophagy
Matsushita, Minako, Suzuki, Nobuo N., Fujioka, Yuko, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
S. cerevisiae Atg5 in complex with the N-terminal regions of Atg16 was expressed, purified and crystallized in four crystal forms.
Crystallization and preliminary crystallographic analysis of human Atg4B–LC3 complex
Satoo, Kenji, Suzuki, Nobuo N., Fujioka, Yuko, Mizushima, Noboru, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
Human Atg4B and LC3 were expressed, purified and crystallized as a complex. Diffraction data were collected to a resolution of 1.9 Å.
Adachi, Wakana, Suzuki, Nobuo N., Fujioka, Yuko, Suzuki, Kuninori, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
Aminopeptidase 1, a cargo protein in the cytosol-to-vacuole targeting (Cvt) pathway, was expressed, purified and crystallized in two crystal forms.
Crystallization and preliminary X-ray analysis of Atg10
Yamaguti, Masaya, Suzuki, Nobuo N., Fujioka, Yuko, Ohsumi, Yoshinori, Inagaki, Fuyuhiko
S. cerevisiae Atg10, an E2-like enzyme that mediates the conjugation reaction between Atg12 and Atg5, was crystallized and diffracted to 2.3 Å resolution.
Ishida, Hiroyuki, Yoshimoto, Kohki, Izumi, Masanori, Reisen, Daniel, Yano, Yuichi, Makino, Amane, ...
During senescence and at times of stress, plants can mobilize needed nitrogen from chloroplasts in leaves to other organs. Much of the total leaf nitrogen is allocated to the most abundant plant...
Wada, Shinya, Ishida, Hiroyuki, Izumi, Masanori, Yoshimoto, Kohki, Ohsumi, Yoshinori, Mae, Tadahiko, ...
Chloroplasts contain approximately 80% of total leaf nitrogen and represent a major source of recycled nitrogen during leaf senescence. While bulk degradation of the cytosol and organelles in plants...