NMR studies of HAC1 mRNA (2009)
Kawahara, Ikumi, Haruta, Kaichiro, Kojima, Chojiro, Tanaka, Yoshiyuki
HAC1 is a transcription factor related to Unfolded Protein Response (UPR) signaling in yeast. Processing of HAC1 mRNA on Endoplasmic reticulum (ER) plays a key role in UPR signaling pathway, but the...
Preparations of hammerhead ribozymes for investigations of their cleavable sequences (2009)
Tateoka, Hisaaki, Kawahara, Ikumi, Hasegawa, Satomi, Haruta, Kaichiro, Kondo, Yoshinori, Kojima, Chojiro, ...
Recently, in hammerhead ribozymes, newly identified loop-loop interaction was found to be important for their activation. Therefore, we chemically synthesized a hammerhead ribozyme with this extra...
Human Genome, Yoshiyuki Tanaka, Takeshi Nagashima, Akihiko Konagaya
The vast increase of genomic sequence data reveals the limitation of single computer systems in terms of both processing and storage capacity. The only realistic way to cope with this growth in the...
NAKAOKA, MASAHIRO, TANAKA, YOSHIYUKI, MUKAI, HIROSHI, SUZUKI, TAKAO, ARYUTHAKA, CHITTIMA
Evaluation of the impact of a catastrophic disturbance on biodiversity is often difficult due to a lack of sufficient quantitative data on biological communities prior to the disturbance. Since 2001,...
Spectroscopic studies on a novel intramolecular hydrogen bond within the (6 4) photoproduct (2007)
Yamamoto, Junpei, Tanaka, Yoshiyuki, Hitomi, Kenichi, Getzoff, Elizabeth D., Iwai, Shigenori
The (6–4) photoproduct, which is one of major UVinduced lesions formed between adjacent pyrimidine bases in DNA, was characterized by using 15N NMR, fluorescence emission, and UV/VIS absorption at...
Structural and physicochemical features on the metal-mediated base pairs (2007)
Tanaka, Yoshiyuki, Oda, Shuji, Yamaguchi, Hiroshi, Haruta, Kaichiro, Kawamura, Takuya, Kondo, Yoshinori, ...
The chemical structure of the mercury-mediated T-T pair (T-HgII-T) was determined with 15N NMR spectroscopy. In order to determine the chemical structure of the T-HgII-T pair, 15N-15N J-coupling...
Synthesis of an enantiomeric DNA oligomer and its T-HgII-T base-pair formation (2007)
Haruta, Kaichiro, Tanaka, Yoshiyuki, Kawamura, Takuya, Kondo, Yoshinori, Ono, Akira, Yamakoshi, Hiroyuki, ...
Unnatural “L-DNA” containing 2′-deoxy-L-ribose backbone instead of natural 2′-deoxy-D-ribose was synthesized and evaluated by 1H NMR, CD spectra. Proton (1H) NMR spectra of natural DNA...
Spectroscopic analysis of AgI-DNA complex (2006)
Oda, Shuji, Komuro, Tomoyuki, Kondo, Yoshinori, Ono, Akira, Tanaka, Yoshiyuki
It has recently been reoprted that C-C mismatches were able to recognize AgI specifically, and form AgI-mediated cytosine-cytosine base pairs (C-AgI-C pair). In this study, we explored DNA sequences,...
Structural analyses on the mercuryII-mediated T-T base pair (2006)
Tanaka, Yoshiyuki, Oda, Shuji, Yamaguchi, Hiroshi, Kudo, Megumi, Kondo, Yoshinori, Kojima, Chojiro, ...
Recently, it was reported that T-T mismatches can specifically recognize HgII, and form T-HgII-T pairs. In order to understand the structure and properties of the T-HgII-T pair, we measured NMR...
Nakamura, Atsuko, Fukuda, Atsunori, Sakai, Shingo, Tanaka, Yoshiyuki
We isolated two cDNA clones (OsCLC-1 and OsCLC-2) homologous to tobacco CLC-Nt1, which encodes a voltage-gated chloride channel, from rice (Oryza sativa L. ssp. japonica, cv. Nipponbare). The deduced...
Nakamura, Atsuko, Fukuda, Atsunori, Sakai, Shingo, Tanaka, Yoshiyuki
We isolated two cDNA clones (OsCLC-1 and OsCLC-2) homologous to tobacco CLC-Nt1, which encodes a voltage-gated chloride channel, from rice (Oryza sativa L. ssp. japonica, cv. Nipponbare). The deduced...
Spectroscopic analyses of DNA duplexes in the presence of mercury ions (2005)
Yamaguchi, Hiroshi, Oda, Shuji, Kojima, Chojiro, Ono, Akira, Kondo, Yoshinori, Tanaka, Yoshiyuki
The DNA duplex with tandem T•T mismatches in the presence of Hg(II) has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T•T mismatches (TT10)....
Tanaka, Yoshiyuki, Yamaguchi, Hiroshi, Oda, Shuji, Kojima, Chojiro, Ono, Akira, Taira, Kazunari, ...
We have studied complexations of functional nucleic acids and metal ions, by means of NMR spectroscopy. In the case of hammerhead ribozymes, they have a metal ion-binding motif in their core...
Miyagishi, Makoto, Matsumoto, Sahohime, Akashi, Hideo, Kawasaki, Hiroaki, Fukao, Taro, Fukuda, Yoko, ...
Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we...
Nakamura, Atsuko, Fukuda, Atsunori, Sakai, Shingo, Tanaka, Yoshiyuki
We isolated two cDNA clones (OsCLC-1 and OsCLC-2) homologous to tobacco CLC-Nt1, which encodes a voltage-gated chloride channel, from rice (Oryza sativa L. ssp. japonica, cv. Nipponbare). The deduced...
Nakaoka, Masahiro, Tanaka, Yoshiyuki, Watanabe, Masako
Seagrass beds are among the most important components in coastal ecosystem, but are susceptible to human-induced changes in terrestrial ecosystem. As a first step to understand the effects of...
Fukuda, Atsunori, Nakamura, Atsuko, Tagiri, Akemi, Tanaka, Hiroshi, Miyao, Akio, Hirochika, Hirohiko, ...
We examined the function and intracellular localization of the product of the Na+/H+ antiporter gene (OsNHX1) cloned from rice (Oryza sativa). OsNHX1 has the ability to suppress Na+,...
Spectroscopic analysis of DNA duplexes includingT-T mismatches (2004)
Yamaguchi, Hiroshi, Oda, Shuji, Kondo, Yoshinori, Ono, Akira, Tanaka, Yoshiyuki
The DNA duplex with tandem T·T mismatches has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T·T mismatches, TT10:d(CGCGTTGTCC) ·...
Fukuda, Atsunori, Chiba, Kazuhiro, Maeda, Miki, Nakamura, Atsuko, Maeshima, Masayoshi, Tanaka, Yoshiyuki
Two cDNA clones encoding vacuolar H+-inorganic pyrophosphatase (HVP1 and HVP10), one clone encoding the catalytic subunit (68 kDa) of vacuolar H+-ATPase (HvVHA-A), and one clone encoding vacuolar...
Fukuda, Atsunori, Chiba, Kazuhiro, Maeda, Miki, Nakamura, Atsuko, Maeshima, Masayoshi, Tanaka, Yoshiyuki
Two cDNA clones encoding vacuolar H+‐inorganic pyrophosphatase (HVP1 and HVP10), one clone encoding the catalytic subunit (68 kDa) of vacuolar H+‐ATPase (HvVHA‐A), and one...
Tanaka, Yoshiyuki, Kasai, Yasuhiro, Morita, Eugene H., Kojima, Chojiro, Toyozawa, Atsushi, Yamasaki, Kazuhiko, ...
Most hammerhead ribozymes have metal ion-binding sequences which are composed of the sheared type G12-A9 pair and the G10.1-C11.1 base-pair. However, in some hammerhead ribozymes, the G10.1-C1l.1...
Tanaka, Yoshiyuki, Kasai, Yasuhiro, Morita, Eugene H., Kojima, Chojiro, Toyozawa, Atsushi, Yamasaki, Kazuhiko, ...
Physicochemical analysis of nucleobase-metal interactions (2002)
Tanaka, Yoshiyuki, Toyozawa, Atsushi, Kondo, Yoshinori
It is important to know what kinds of chemical bonds are formed between metal ions and nucleic acids. We have studied metal-nucleobase interactions, by using NMR spectroscopy in solution. For the...
Kasai, Yasuhiro, Tanaka, Yoshiyuki, Morita, Eugene H., Tanaka, Yoshimasa, Taira, Kazunari
Hammerhead ribozymes have a metal-ion-binding motif in the conserved core region, and this motif can capture several kinds of metal ions. In order to determine what kind of metal ions can be...
Kachi, Shu, Yamazaki, Matsuyo, Tanaka, Yoshiyuki, Hayashi, Fumio, Miyake, Yozo, Wakabayashi, Takashi, ...
Cyclic GMP phosphodiesterase (PDE), a key enzyme for phototransduction, contains two catalytic subunits, Pα and Pβ, and two identical regulatory subunits, Pγs. Neither the structure of the...
Probing of the secondary structure of maxizymes (1999)
Zhou, Jing-Min, Nakamatsu, Yuka, Kuwabara, Tomoko, Warashina, Masaki, Tanaka, Yoshiyuki, Yoshinari, Koichi, ...
The protein encoded by chimeric BCR-ABL mRNA causes chronic myelogenous leukemia (CML). We showed previously that a novel allosterically controllable ribozyme, of the type known as a maxizyme, can...
Action of metal ions directly involved in the cleavage reaction of hammerhead ribozymes (1999)
Nakamatsu, Yuka, Warashina, Masaki, Kuwabara, Tomoko, Tanaka, Yoshiyuki, Shibata, Atsushi, Yoshinari, Koichi, ...
Recently, hammerhead ribozyme-mediated cleavage was analyzed as a function of the concentration of La3+ ions in the presence of a fixed concentration of Mg2+ ions so that the role could be...
Morita, Miyo Terao, Tanaka, Yoshiyuki, Kodama, Takashi S., Kyogoku, Yoshimasa, Yanagi, Hideki, Yura, Takashi
Ohmiya, Akemi, Tanaka, Yoshiyuki, Kadowaki, Koh-ichi, Hayashi, Tateki
An auxin-binding protein (ABP) was previously isolated from shoot apices of peach trees to homogenity on standard SDS-PAGE. Analysis of low-bis SDS-PAGE and direct peptide sequencing of purified...
Maruyama, Chio, Tanaka, Yoshiyuki, Takeyasu, Kunio, Yoshida, Masasuke, Sato, Masa H.
We determined the amino acid residues of the H+-translocating inorganic pyrophosphatase (H+-PPase) of pumpkin which are covalently labeled by two fluorescent labeling reagents;...
Na+/H+ Antiporter in Tonoplast Vesicles from Rice Roots (1998)
Fukuda, Atsunori, Yazaki, Yoshiaki, Ishikawa, Takayuki, Koike, Setsuo, Tanaka, Yoshiyuki
The Na+/H + antiporter in vacuolar membranes transports Na+ from the cytoplasm to vacuoles using a pH gradient generated by proton pumps; it is considered to be related to salinity tolerance. Rice...
Matsuda, Narumi, Tsuchiya, Tohru, Kishitani, Sachie, Tanaka, Yoshiyuki, Toriyama, Kinya
Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) catalyzes the first step in the synthesis of phenylpropanoids. An antisense or sense PAL cDNA of sweet potato under the control of a tapetum-specific...
Molecular Cloning and Analysis of the cDNA for an Auxin-Regulated Calmodulin Gene (1995)
Okamoto, Hitoshi, Tanaka, Yoshiyuki, Sakai, Shingo
An auxin-regulated calmodulin cDNA (arCaM) were isolated by differential screening from a mung bean (Vigna radiata) cDNA library. The expression of the arCaM transcript in the etiolated mung bean...
Sequential Induction of mRNAs for Phenylalanine Ammonia-lyase in Slices of Potato Tuber (1991)
Ishizuka, Masakatsu, Yamada, Fujiko, Tanaka, Yoshiyuki, Takeuchi, Yasuyoshi, Imaseki, Hidemasa
Induction of the mRNA that encodes for phenylalanine ammonia-lyase (PAL, EC 4.3.1.5), which catalyzes the first reaction in the biosynthesis of a wide variety of phenylpropanoid natural products from...
Matsuoka, Makoto, Kano-Murakami, Yuriko, Tanaka, Yoshiyuki, Ozeki, Yoshihiro, Yamamoto, Naoki
Fifteen cDNA clones for the small subunit of ribulose-1,5-bisphosphate carboxylase from rice were grouped into two classes based on restriction enzyme maps. One cDNA insert hybridized to a few DNA...
MATSUOKA, Makoto, KANO-MURAKAMI, Yuriko, TANAKA, Yoshiyuki, OZEKI, Yoshihiro, YAMAMOTO, Naoki
We have cloned a full length cDNA for the small subunit of ribulose-l,5-bisphosphate carboxylase from C4 mońocot maize, determined the complete nucleotide sequence of this cDNA and deduced its...
OZEKI, Yoshihiro, SAKANO, Katsuhiro, KOMAMINE, Atsushi, TANAKA, Yoshiyuki, NOGUCHI, Hiroshi, SANKAWA, Ushio, ...
Chalcone synthase was purified to homogeneity by polyacrylamide gel electrophoresis from cell suspension cultures of carrot in which anthocyanin synthesis was induced by transferring the cells from a...
TAKABE, Teruhiro, ISHKAWA, Hiroshi, NIWA, Satsuki, TANAKA, Yoshiyuki
Chemically modified spinach plastocyanin, in which negatively charged carboxyl residues are replaced with positively charged amino residues, has been prepared. Four distinct species of chemically...
Specific volume and viscosity of methanol-water mixtures under high pressure (1980)
Kubota, Hironobu, Tsuda, Sadahiro, Murata, Masahiro, Yamamoto, Takeshi, Tanaka, Yoshiyuki, Makita, Tadashi
Dielectric constant and density of cyclohexane-benzene mixtures under high pressure (1980)
Kashiwagi, Hiroshi, Fukunaga, Tomiaki, Tanaka, Yoshiyuki, Kubota, Hironobu, Makita, Tadashi
Some evaluated thermophysical properties of gaseous ethyne (1979)
Takezaki, Yoshimasa, Watanabe, Koichi, Tanaka, Yoshiyuki, Nagashima, Akira
Polar transport and content of indole-3-acetic acid in wounded sweet potato root tissues (1979)
Tanaka, Yoshiyuki, Uritani, Ikuzo
In roots of sweet potato (Ipomoea batatas Lam. cv. Kokei 14), the metabolic response to wounding was remarkable in the proximal side and developed in the acropetal direction. We assumed that the...
Tanaka, Yoshiyuki, Uritani, Ikuzo
In roots of sweet potato (Ipomoea batatas Lam. cv. Kokei 14), the metabolic response to wounding was remarkable only in the proximal side. We assumed that the polarity resulted from a polar movement...
Specific volume and viscosity of ethanol-water mixtures under high pressure (1977)
Tanaka, Yoshiyuki, Yamamoto, Takeshi, Satomi, Yoshimasa, Kubota, Hironobu, Makita, Tadashi
Purification and Properties of Phenylalanine Ammonia-Lyase in Cut-injured Sweet Potato (1977)
TANAKA, Yoshiyuki, URITANI, Ikuzo
L-Phenylalanine ammonia-lyase (PAL) activity was developed in response to cut injury in sweet potato root tissue. The enzyme was purified from tissue incubated for 1 day after slicing by ammonium...
Tanaka, Yoshiyuki, Matsushita, Kazunobu, Uritani, Ikuzo
In sweet potato roots, activity of the phenylalanine ammonia-lyase (PAL)-inactivating system in crude enzyme solution increased markedly in response to cut injury after a lag period of about 10 hr...
TANAKA, Yoshiyuki, URITANI, Ikuzo
Antibody toward phenylalanine ammonia-lyase (PAL) [EC 4.3.1.5] was obtained by immunization of a rabbit with highly purified PAL. The antibody reacted specifically with RAL, as demonstrated by the...
Tanaka, Yoshiyuki, Kojima, Mineo, Uritani, Ikuzo
In sweet potato root tissue, cinnamic acid 4-hydroxylase activity increased markedly in response to cut injury, and reached a maximum after 1 day of incubation. The patterns of development and...
Preparation and chemical properties of cubic boron arsenide, Bas (1966)
Osugi, Jiro, Shimizu, Kiyoshi, Tanaka, Yoshiyuki, Kadono, Kosaku
Morita, Miyo Terao, Tanaka, Yoshiyuki, Kodama, Takashi S., Kyogoku, Yoshimasa, Yanagi, Hideki, Yura, Takashi
Induction of heat shock proteins in Escherichia coli is primarily caused by increased cellular levels of the heat shock σ-factor σ32 encoded by the rpoH gene. Increased σ32 levels result from both...
Tanaka, Yoshiyuki, Uritani, Ikuzo
Investigation of polyphenol production in cut-injured sweet potato (Ipomoea batatas Lam. cv. Kokei 14) roots by histochemical and quantitative methods showed that polyphenols were produced in...
Classification and Structural Comparison of Full-Length cDNAs for Pathogenesis-Related Proteins 1
Matsuoka, Makoto, Yamamoto, Naoki, Kano-Murakami, Yuriko, Tanaka, Yoshiyuki, Ozeki, Yoshihiro, Hirano, Hisashi, ...
Fourteen cDNA clones of pathogenesis-related (PR) proteins, PR1a and PR1b of tobacco were obtained and classified into six groups based on restriction enzyme maps. To assign the groups to different...
Tanaka, Yoshiyuki, Matsuoka, Makoto, Yamanoto, Naoki, Ohashi, Yuko, Kano-Murakami, Yuriko, Ozeki, Yoshihiro
A cDNA clone for phenylalanine ammonia-lyase (PAL) induced in wounded sweet potato (Ipomoea batatas Lam.) root was obtained by immunoscreening a cDNA library. The protein produced in Escherichia coli...
Koizuka, Nobuya, Tanaka, Yoshiyuki, Morohashi, Yukio
A cDNA was isolated that codes for α-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about...
Light-Independent Expression of cab and rbcS Genes in Dark-Grown Pine Seedlings 1
Yamamoto, Naoki, Mukai, Yuzuru, Matsuoka, Makoto, Kano-Murakami, Yuriko, Tanaka, Yoshiyuki, Ohashi, Yuko, ...
In angiosperms, light has been shown to induce the expression of cab and rbcS genes, which encode the apoprotein of light-harvesting chlorophyll a/b binding protein (LHCP) and the small subunit of...
Morita, Miyo Terao, Tanaka, Yoshiyuki, Kodama, Takashi S., Kyogoku, Yoshimasa, Yanagi, Hideki, Yura, Takashi
Induction of heat shock proteins in Escherichia coli is primarily caused by increased cellular levels of the heat shock σ-factor σ32 encoded by the rpoH gene. Increased σ32 levels result from both...
Tanaka, Yoshiyuki, Uritani, Ikuzo
Investigation of polyphenol production in cut-injured sweet potato (Ipomoea batatas Lam. cv. Kokei 14) roots by histochemical and quantitative methods showed that polyphenols were produced in...
Classification and Structural Comparison of Full-Length cDNAs for Pathogenesis-Related Proteins 1
Matsuoka, Makoto, Yamamoto, Naoki, Kano-Murakami, Yuriko, Tanaka, Yoshiyuki, Ozeki, Yoshihiro, Hirano, Hisashi, ...
Fourteen cDNA clones of pathogenesis-related (PR) proteins, PR1a and PR1b of tobacco were obtained and classified into six groups based on restriction enzyme maps. To assign the groups to different...
Tanaka, Yoshiyuki, Matsuoka, Makoto, Yamanoto, Naoki, Ohashi, Yuko, Kano-Murakami, Yuriko, Ozeki, Yoshihiro
A cDNA clone for phenylalanine ammonia-lyase (PAL) induced in wounded sweet potato (Ipomoea batatas Lam.) root was obtained by immunoscreening a cDNA library. The protein produced in Escherichia coli...
Koizuka, Nobuya, Tanaka, Yoshiyuki, Morohashi, Yukio
A cDNA was isolated that codes for α-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about...
Light-Independent Expression of cab and rbcS Genes in Dark-Grown Pine Seedlings 1
Yamamoto, Naoki, Mukai, Yuzuru, Matsuoka, Makoto, Kano-Murakami, Yuriko, Tanaka, Yoshiyuki, Ohashi, Yuko, ...
In angiosperms, light has been shown to induce the expression of cab and rbcS genes, which encode the apoprotein of light-harvesting chlorophyll a/b binding protein (LHCP) and the small subunit of...
Obata, Toshihiro, Kitamoto, Hiroko K., Nakamura, Atsuko, Fukuda, Atsunori, Tanaka, Yoshiyuki
We screened a rice (Oryza sativa L. ‘Nipponbare’) full-length cDNA expression library through functional complementation in yeast (Saccharomyces cerevisiae) to find novel cation transporters...