Perlova, Olena, Gerth, Klaus, Kuhlmann, Silvia, Zhang, Youming, Müller, Rolf
Abstract Although many secondary metabolites with diverse biological activities have been isolated from myxobacteria, most strains of these biotechnologically important gliding prokaryotes remain...
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals (2008)
Poser, Ina, Sarov, Mihail, Hutchins, James R A, Heriche, Jean-Karim, Toyoda, Yusuke, Pozniakovsky, Andrei, ...
The interpretation of genome sequences requires reliable and standardized methods to assess protein function at high throughput. Here we describe a fast and reliable pipeline to study protein...
Fu, Jun, Wenzel, Silke C., Perlova, Olena, Wang, Junping, Gross, Frank, Tang, Zhiru, ...
Horizontal gene transfer by transposition has been widely used for transgenesis in prokaryotes. However, conjugation has been preferred for transfer of large transgenes, despite greater restrictions...
A recombineering pipeline for functional genomics applied to Caenorhabditis elegans (2006)
Sarov, Mihail, Schneider, Susan, Pozniakovski, Andrei, Roguev, Assen, Ernst, Susanne, Zhang, Youming, ...
We present a new concept in DNA engineering based on a pipeline of serial recombineering steps in liquid culture. This approach is fast, straightforward and facilitates simultaneous processing of...
Leah C. Solberg, William Valdar, Dominique Gauguier, Graciela Nunez, Amy Taylor, Stephanie Burnett, ...
in mice
NOD1 variation, immunoglobulin E and asthma (2005)
Hysi, Pirro, Kabesch, Michael, Moffatt, Miriam F., Schedel, Michaela, Carr, David, Zhang, Youming, ...
Asthma is a familial inflammatory disease of the airways of the lung. Microbial exposures in childhood protect against asthma through unknown mechanisms. The innate immune system is able to identify...
NOD1 variation, Immunoglobulin E, and asthma (2005)
Hysi, Pirro, Kabesch, Michael, Moffatt, Miriam F., Schedel, Michaela, Carr, David, Zhang, Youming, ...
Asthma is a familial inflammatory disease of the airways of the lung. Microbial exposures in childhood protect against asthma through unknown mechanisms. The innate immune system is able to identify...
Zhang, Youming, Muyrers, Joep PP, Rientjes, Jeanette, Stewart, A Francis
Abstract Background The phage protein pairs, RecE/RecT from Rac or Redα/Redβ from λ, initiate efficient double strand break repair (DSBR) in Escherichia coli that has proven very useful for DNA...
Zhang, Youming, Lefort, Jean, Kearsey, Virginia, Lapa E Silva, José Roberto, Vargaftig, B. Boris
Asthma is the most common illness of childhood, affecting one child in seven in the UK. Asthma has a genetic basis, but genetic studies of asthma in humans are confounded by uncontrolled...
Muyrers, Joep P.P., Zhang, Youming, Buchholz, Fraenk, Stewart, A. Francis
The initial steps of double-stranded break (DSB) repair by homologous recombination mediated by the 5′–3′ exonuclease/annealing protein pairs, RecE/RecT and Redα/Redβ, were analyzed....
Point mutation of bacterial artificial chromosomes by ET recombination
Muyrers, Joep P.P., Zhang, Youming, Benes, Vladimir, Testa, Giuseppe, Ansorge, Wilhelm, Stewart, A. Francis
Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow...
Extent and Distribution of Linkage Disequilibrium in Three Genomic Regions
Abecasis, Gonçalo R., Noguchi, Emiko, Heinzmann, Andrea, Traherne, James A., Bhattacharyya, Sumit, Leaves, Nicholas I., ...
The positional cloning of genes underlying common complex diseases relies on the identification of linkage disequilibrium (LD) between genetic markers and disease. We have examined 127 polymorphisms...
Muyrers, Joep P.P., Zhang, Youming, Buchholz, Fraenk, Stewart, A. Francis
The initial steps of double-stranded break (DSB) repair by homologous recombination mediated by the 5′–3′ exonuclease/annealing protein pairs, RecE/RecT and Redα/Redβ, were analyzed....
Point mutation of bacterial artificial chromosomes by ET recombination
Muyrers, Joep P.P., Zhang, Youming, Benes, Vladimir, Testa, Giuseppe, Ansorge, Wilhelm, Stewart, A. Francis
Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow...
Extent and Distribution of Linkage Disequilibrium in Three Genomic Regions
Abecasis, Gonçalo R., Noguchi, Emiko, Heinzmann, Andrea, Traherne, James A., Bhattacharyya, Sumit, Leaves, Nicholas I., ...
The positional cloning of genes underlying common complex diseases relies on the identification of linkage disequilibrium (LD) between genetic markers and disease. We have examined 127 polymorphisms...
Perlova, Olena, Fu, Jun, Kuhlmann, Silvia, Krug, Daniel, Stewart, A. Francis, Zhang, Youming, ...
Although many secondary metabolites exhibiting important pharmaceutical and agrochemical activities have been isolated from myxobacteria, most of these microorganisms remain difficult to handle...
Fu, Jun, Wenzel, Silke C., Perlova, Olena, Wang, Junping, Gross, Frank, Tang, Zhiru, ...
Horizontal gene transfer by transposition has been widely used for transgenesis in prokaryotes. However, conjugation has been preferred for transfer of large transgenes, despite greater restrictions...
Perlova, Olena, Gerth, Klaus, Kuhlmann, Silvia, Zhang, Youming, Müller, Rolf
Although many secondary metabolites with diverse biological activities have been isolated from myxobacteria, most strains of these biotechnologically important gliding prokaryotes remain difficult to...